Redefining pseudokinases: A look at the untapped enzymatic potential of pseudokinases
AbstractCatalytically inactive kinases, known as pseudokinases, are conserved in all three domains of life. Due to the lack of catalytic residues, pseudokinases are considered to act as allosteric regulators and scaffolding proteins with no enzymatic function. However, since these “dead” kinases are conserved along with their active counterparts, a role for pseudokinases may have been overlooked. In this review, we will discuss the recently characterized pseudokinases Selenoprotein O,Legionella effector SidJ, and the SARS-CoV2 protein nsp12 which catalyze AMPylation, glutamylation, and RNAylation, respectively. These s...
Source: IUBMB Life - January 5, 2023 Category: Research Authors: Alex Pon,
Adam Osinski,
Anju Sreelatha Tags: SPECIAL ISSUE Source Type: research
The roles of proteases in prostate cancer
AbstractSince the proposition of the pro-invasive activity of proteolytic enzymes over 70 years ago, several roles for proteases in cancer progression have been established. About half of the 473 active human proteases are expressed in the prostate and many of the most well-characterized members of this enzyme family are regulated by androgens, hormones essential for development of pr ostate cancer. Most notably, several kallikrein-related peptidases, including KLK3 (prostate-specific antigen, PSA), the most well-known prostate cancer marker, and type II transmembrane serine proteases, such as TMPRSS2 and matriptase, ha...
Source: IUBMB Life - January 5, 2023 Category: Research Authors: Hannu Koistinen,
Ruusu ‐Maaria Kovanen,
Morley D. Hollenberg,
Antoine Dufour,
Evette S. Radisky,
Ulf‐Håkan Stenman,
Jyotsna Batra,
Judith Clements,
John D. Hooper,
Eleftherios Diamandis,
Oliver Schilling,
Antti Rannikko,
Tuomas Mirtti Tags: CRITICAL REVIEW Source Type: research
Issue Information
(Source: IUBMB Life)
Source: IUBMB Life - December 27, 2022 Category: Research Tags: ISSUE INFORMATION Source Type: research
The latest achievements in biochemistry, biophysics and biotechnology —50 years of history of the Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Kraków, Poland
(Source: IUBMB Life)
Source: IUBMB Life - December 27, 2022 Category: Research Authors: Dulak Jozef Tags: EDITORIAL Source Type: research
Issue Information
(Source: IUBMB Life)
Source: IUBMB Life - December 27, 2022 Category: Research Tags: ISSUE INFORMATION Source Type: research
The latest achievements in biochemistry, biophysics and biotechnology —50 years of history of the Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Kraków, Poland
(Source: IUBMB Life)
Source: IUBMB Life - December 27, 2022 Category: Research Authors: Dulak Jozef Tags: EDITORIAL Source Type: research
Thiourea derivatives inhibit key diabetes ‐associated enzymes and advanced glycation end‐product formation as a treatment for diabetes mellitus
This study was designed to screen novel thiourea derivatives against different enzymes, such as α-amylase, α-glucosidase, protein tyrosine phosphatase 1 B, and advanced glycated end product (AGEs). A cytotoxicity analysis was performed using rat L6 myotubes and molecular docking analysis was performed to map the binding interactions between the active compounds and α-amylase and α-glucosid ase. The data revealed the potency of five compounds, including E (1-(2,4-difluorophenyl)-3-(3,4-dimethyl phenyl) thiourea), AG (1-(2-methoxy-5-(trifluoromethyl) phenyl)-3-(3-methoxy phenyl) thiourea), AF (1-(2,4-dichlorophenyl)-3-(4...
Source: IUBMB Life - December 25, 2022 Category: Research Authors: Imran Ullah,
Mukhtiar Hassan,
Khalid M. Khan,
Muhammad Sajid,
Muhammad Umar,
Said Hassan,
Amin Ullah,
Hamed A. El ‐Serehy,
Wafa Charifi,
Humaira Yasmin Tags: RESEARCH ARTICLE Source Type: research
Kinetochore ‐catalyzed MCC formation: A structural perspective
AbstractThe spindle assembly checkpoint (SAC) is a cellular surveillance mechanism that functions to ensure accurate chromosome segregation during mitosis. Macromolecular complexes known as kinetochores, act as the interface of sister chromatid attachment to spindle microtubules. In response to unattached kinetochores, the SAC activates its effector, the mitotic checkpoint complex (MCC), which delays mitotic exit until all sister chromatid pairs have achieved successful attachment to the bipolar mitotic spindle. Formation of the MCC (composed of Mad2, BubR1, Bub3 and Cdc20) is regulated by an Mps1 kinase-dependent phosphor...
Source: IUBMB Life - December 17, 2022 Category: Research Authors: Elyse S. Fischer Tags: SPECIAL ISSUE Source Type: research
Thirteen dubious ways to detect conserved structural RNAs
AbstractCovariation induced by compensatory base substitutions in RNA alignments is a great way to deduce conserved RNA structure, in principle. In practice, success depends on many factors, importantly the quality and depth of the alignment and the choice of covariation statistic. Measuring covariation between pairs of aligned positions is easy. However, using covariation to infer evolutionarily conserved RNA structure is complicated by other extraneous sources of covariation such as that resulting from homologous sequences having evolved from a common ancestor. In order to provide evidence of evolutionarily conserved RNA...
Source: IUBMB Life - December 13, 2022 Category: Research Authors: William Gao,
Ann Yang,
Elena Rivas Tags: SPECIAL ISSUE Source Type: research
