Issue highlights
IUBMB Life, Volume 72, Issue 5, Page 813-813, May 2020. (Source: IUBMB Life)
Source: IUBMB Life - April 3, 2020 Category: Research Tags: ISSUE HIGHLIGHTS Source Type: research

Issue Information
IUBMB Life, Volume 72, Issue 5, Page 805-812, May 2020. (Source: IUBMB Life)
Source: IUBMB Life - April 3, 2020 Category: Research Tags: ISSUE INFORMATION Source Type: research

Autophagy regulation by microRNAs: Novel insights into osteosarcoma therapy
AbstractOsteosarcoma (OS) is a kind of primary bone cancer that is considered as the leading cause of children death. Surgery and chemotherapy are considered as common treatment approaches for OS; the rate of survival for patients is almost 60 –70%. Besides the used therapeutic approaches, it seems that there is a crucial need to launch new treatments for OS. In this regard, more understanding about cellular and molecular pathways involved in OS can contribute to recovery and develop new therapeutic platforms. Autophagy is a cellular ma chinery that digests and degrades dysfunctional proteins and organelles, so it ca...
Source: IUBMB Life - March 31, 2020 Category: Research Authors: Zeinab Jamali, Mortaza Taheri ‐Anganeh, Zahra Shabaninejad, Abdolkhalegh Keshavarzi, Hajar Taghizadeh, Zahra Sadat Razavi, Reza Mottaghi, Mohammadreza Abolhassan, Ahmad Movahedpour, Hamed Mirzaei Tags: CRITICAL REVIEW Source Type: research

R ‐spondin‐mediated WNT signaling potentiation in mammary and breast cancer development
AbstractThe mammary gland is a secretory organ, which develops as a network of growing epithelial ducts composed of luminal and basal cells that invade the surrounding adipose tissue through a series of developmental cycles. Mammary stem cells (MaSCs) maintain an accurate tissue homeostasis, and their proliferation and cell fate determination are regulated by multiple hormones and local factors. The WNT pathway plays a critical role in controlling the enormous tissue expansion and remodeling during mammary gland development through the maintenance and differentiation of MaSCs, and its deregulation has been implicated in br...
Source: IUBMB Life - March 31, 2020 Category: Research Authors: Johanna M. Tocci, Carla M. Felcher, Mart ín E. García Solá, Edith C. Kordon Tags: CRITICAL REVIEW Source Type: research

Autophagy regulation by microRNAs: Novel insights into osteosarcoma therapy
AbstractOsteosarcoma (OS) is a kind of primary bone cancer that is considered as the leading cause of children death. Surgery and chemotherapy are considered as common treatment approaches for OS; the rate of survival for patients is almost 60 –70%. Besides the used therapeutic approaches, it seems that there is a crucial need to launch new treatments for OS. In this regard, more understanding about cellular and molecular pathways involved in OS can contribute to recovery and develop new therapeutic platforms. Autophagy is a cellular ma chinery that digests and degrades dysfunctional proteins and organelles, so it ca...
Source: IUBMB Life - March 31, 2020 Category: Research Authors: Zeinab Jamali, Mortaza Taheri ‐Anganeh, Zahra Shabaninejad, Abdolkhalegh Keshavarzi, Hajar Taghizadeh, Zahra Sadat Razavi, Reza Mottaghi, Mohammadreza Abolhassan, Ahmad Movahedpour, Hamed Mirzaei Tags: CRITICAL REVIEW Source Type: research

R ‐spondin‐mediated WNT signaling potentiation in mammary and breast cancer development
AbstractThe mammary gland is a secretory organ, which develops as a network of growing epithelial ducts composed of luminal and basal cells that invade the surrounding adipose tissue through a series of developmental cycles. Mammary stem cells (MaSCs) maintain an accurate tissue homeostasis, and their proliferation and cell fate determination are regulated by multiple hormones and local factors. The WNT pathway plays a critical role in controlling the enormous tissue expansion and remodeling during mammary gland development through the maintenance and differentiation of MaSCs, and its deregulation has been implicated in br...
Source: IUBMB Life - March 31, 2020 Category: Research Authors: Johanna M. Tocci, Carla M. Felcher, Mart ín E. García Solá, Edith C. Kordon Tags: CRITICAL REVIEW Source Type: research

microRNA ‐495 reduces visceral sensitivity in mice with diarrhea‐predominant irritable bowel syndrome through suppression of the PI3K/AKT signaling pathway via PKIB
AbstractDiarrhea ‐predominant irritable bowel syndrome (IBS‐D) is one of the most common gastrointestinal disorders in the world, lacking effective therapies. The crucial roles of microRNAs (miRNAs) in IBS‐D have attracted increasing attention. The aim of this study is to investigate the effects of miR‐495 o n the visceral sensitivity of the IBS‐D through the PI3K/AKT signaling pathway by targeting PKIB. Microarray data analysis was employed to screen the differentially expressed genes related to IBS‐D and regulatory miRNAs. Then, mice were perfused with acetic acid into the rectum to establish the IBS‐D mode...
Source: IUBMB Life - March 30, 2020 Category: Research Authors: Lifeng Fei, Yanjing Wang Tags: RESEARCH COMMUNICATION Source Type: research

Lack of miR ‐1246 in small extracellular vesicle blunts tumorigenesis of laryngeal carcinoma cells by regulating Cyclin G2
AbstractSmall extracellular vesicle (sEV) has precise impacts on tumor microenvironment and play vital functions in intercellular interaction. However, the functional role of sEV miRNA on laryngeal squamous cell carcinoma (LSCC) is largely unresolved. Here, the expression of miR ‐1246 in LSCC tissues and plasma sEV was examined. The internalization ability of sEV was determined by uptake assay. Then, the source and purity of sEV were checked through RNase and/or pharmacological inhibitors application. The invasion, migration, proliferation, and cell cycle assays were used to determine the altered abilities of miR‐1246 ...
Source: IUBMB Life - March 30, 2020 Category: Research Authors: Qiang Huang, Chi ‐Yao Hsueh, Yang Guo, Xiu‐Fa Wu, Jiao‐Yu Li, Liang Zhou Tags: RESEARCH COMMUNICATION Source Type: research

How hypoxia regulate exosomes in ischemic diseases and cancer microenvironment?
AbstractExosomes, as natural occurring vesicles, play highly important roles in the behavior and fate of ischemic diseases and different tumors. Secretion, composition, and function of exosomes are remarkably influenced by hypoxia in ischemic diseases and tumor microenvironment. Exosomes secreted from hypoxic cells affect development, growth, angiogenesis, and progression in ischemic diseases and tumors through a variety of signaling pathways. In this review article, we discuss how hypoxia affects the quantity and quality of exosomes, and review the mechanisms by which hypoxic cell ‐derived exosomes regulate ischemic cel...
Source: IUBMB Life - March 30, 2020 Category: Research Authors: Sajad Yaghoubi, Hamid Najminejad, Mehran Dabaghian, Mohammad Hossein Karimi, Meghdad Abdollahpour ‐Alitappeh, Fariba Rad, Motahareh Mahi‐Birjand, Shiva Mohammadi, Fatemeh Mohseni, Mohammad Sobhani Lari, Gholam Hossein Teymouri, Esmat Rigi Y Tags: CRITICAL REVIEW Source Type: research

Human and Arabidopsis alpha ‐ketoglutarate‐dependent dioxygenase homolog proteins—New players in important regulatory processes
AbstractThe family of AlkB homolog (ALKBH) proteins, the homologs ofEscherichia coli AlkB 2 ‐oxoglutarate (2OG), and Fe(II)‐dependent dioxygenase are involved in a number of important regulatory processes in eukaryotic cells including repair of alkylation lesions in DNA, RNA, and nucleoprotein complexes. There are nine human and thirteenArabidopsis thaliana ALKBH proteins described, which exhibit diversified functions. Among them, human ALKBH5 and FaT mass and Obesity ‐associated (FTO) protein and Arabidopsis ALKBH9B and ALKBH10B have been recognized as N6 methyladenine (N6meA) demethylases, the most abundant posttra...
Source: IUBMB Life - March 30, 2020 Category: Research Authors: Micha ł Marcinkowski, Tomaš Pilžys, Damian Garbicz, Jaroslaw Steciuk, Dorota Zugaj, Damian Mielecki, Tomasz J. Sarnowski, Elżbieta Grzesiuk Tags: SPECIAL ISSUE Source Type: research

Lack of miR ‐1246 in small extracellular vesicle blunts tumorigenesis of laryngeal carcinoma cells by regulating Cyclin G2
AbstractSmall extracellular vesicle (sEV) has precise impacts on tumor microenvironment and play vital functions in intercellular interaction. However, the functional role of sEV miRNA on laryngeal squamous cell carcinoma (LSCC) is largely unresolved. Here, the expression of miR ‐1246 in LSCC tissues and plasma sEV was examined. The internalization ability of sEV was determined by uptake assay. Then, the source and purity of sEV were checked through RNase and/or pharmacological inhibitors application. The invasion, migration, proliferation, and cell cycle assays were used to determine the altered abilities of miR‐1246 ...
Source: IUBMB Life - March 21, 2020 Category: Research Authors: Qiang Huang, Chi ‐Yao Hsueh, Yang Guo, Xiu‐Fa Wu, Jiao‐Yu Li, Liang Zhou Tags: RESEARCH COMMUNICATION Source Type: research

How hypoxia regulate exosomes in ischemic diseases and cancer microenvironment?
AbstractExosomes, as natural occurring vesicles, play highly important roles in the behavior and fate of ischemic diseases and different tumors. Secretion, composition, and function of exosomes are remarkably influenced by hypoxia in ischemic diseases and tumor microenvironment. Exosomes secreted from hypoxic cells affect development, growth, angiogenesis, and progression in ischemic diseases and tumors through a variety of signaling pathways. In this review article, we discuss how hypoxia affects the quantity and quality of exosomes, and review the mechanisms by which hypoxic cell ‐derived exosomes regulate ischemic cel...
Source: IUBMB Life - March 21, 2020 Category: Research Authors: Sajad Yaghoubi, Hamid Najminejad, Mehran Dabaghian, Mohammad Hossein Karimi, Meghdad Abdollahpour ‐Alitappeh, Fariba Rad, Motahareh Mahi‐Birjand, Shiva Mohammadi, Fatemeh Mohseni, Mohammad Sobhani Lari, Gholam Hossein Teymouri, Esmat Rigi Y Tags: CRITICAL REVIEW Source Type: research

microRNA ‐495 reduces visceral sensitivity in mice with diarrhea‐predominant irritable bowel syndrome through suppression of the PI3K/AKT signaling pathway via PKIB
AbstractDiarrhea ‐predominant irritable bowel syndrome (IBS‐D) is one of the most common gastrointestinal disorders in the world, lacking effective therapies. The crucial roles of microRNAs (miRNAs) in IBS‐D have attracted increasing attention. The aim of this study is to investigate the effects of miR‐495 o n the visceral sensitivity of the IBS‐D through the PI3K/AKT signaling pathway by targeting PKIB. Microarray data analysis was employed to screen the differentially expressed genes related to IBS‐D and regulatory miRNAs. Then, mice were perfused with acetic acid into the rectum to establish the IBS‐D mode...
Source: IUBMB Life - March 19, 2020 Category: Research Authors: Lifeng Fei, Yanjing Wang Tags: RESEARCH COMMUNICATION Source Type: research

Issue highlights
IUBMB Life, Volume 72, Issue 4, Page 523-523, April 2020. (Source: IUBMB Life)
Source: IUBMB Life - March 18, 2020 Category: Research Tags: ISSUE HIGHLIGHTS Source Type: research

Issue Information
IUBMB Life, Volume 72, Issue 4, Page 515-522, April 2020. (Source: IUBMB Life)
Source: IUBMB Life - March 18, 2020 Category: Research Tags: ISSUE INFORMATION Source Type: research

A competition between hydrophobic and electrostatic interactions in protein –ligand systems. Binding of heterogeneously halogenated benzotriazoles by the catalytic subunit of human protein kinase CK2
AbstractA series of chlorine ‐substituted benzotriazole derivatives, representing all possible substitution patterns of halogen atoms attached to the benzotriazole benzene ring, were synthetized as potential inhibitors of human protein kinase CK2. Basic ADME parameters for the free solutes (hydrophobicity, electronic properti es) together with their binding affinity to the catalytic subunit of protein kinase CK2 were determined with reverse‐phase HPLC, spectrophotometric titration, and Thermal Shift Assay Method, respectively. The analysis of position‐dependent thermodynamic contribution of a chlorine atom attached t...
Source: IUBMB Life - March 18, 2020 Category: Research Authors: S ławomir Kasperowicz, Ewa Marzec, Agnieszka M. Maciejewska, Damian Trzybiński, Maria Bretner, Krzysztof Woźniak, Jarosław Poznański, Kinga Mieczkowska Tags: SPECIAL ISSUE Source Type: research

Circulating levels of C1q/TNF ‐α‐related protein 6 (CTRP6) in polycystic ovary syndrome
AbstractPolycystic ovary syndrome (PCOS) is one of the most common endocrine disorders affecting females of reproductive age. It has been associated with cardiometabolic disorders including diabetes mellitus and cardiovascular disorders, and increases the risk of developing fecundity pathologies including recurrent pregnancy loss (RPL) and infertility. C1q/tumor necrosis factor ‐α‐related protein‐6 (CTRP6) is a novel adipokine involved in glucose and lipid metabolism, host inflammation, and organogenesis. In the present study, we aimed to determine the association of serum CTRP6 levels with some components of m...
Source: IUBMB Life - March 18, 2020 Category: Research Authors: Asie Sadeghi, Reza Fadaei, Nariman Moradi, Fatima Z. Fouani, Maryam Roozbehkia, Zahra Zandieh, Soheila Ansaripour, Akram Vatannejad, Amir H. Doustimotlagh Tags: RESEARCH COMMUNICATION Source Type: research

MicroRNA ‐34a‐mediated death of acute myeloid leukemia stem cells through apoptosis induction and exosome shedding inhibition via histone deacetylase 2 targeting
AbstractWe investigated the role of leukemia stem cells in chemoresistance and recurrence of acute myeloid leukemia. Total RNA was isolated from cells or tissues using TRIzol reagent. Cell viability was assessed with the tetrazolium assay. MicroRNA ‐34a (miR‐34a), which acts on cell death regulation pathways, was noticeably downregulated in non‐M3 acute myeloid leukemia stem cells compared with normal hematopoietic stem cells. Furthermore, inhibition of miR‐34a‐mediated suppression in leukemia stem cells was associated with poor clin ical outcomes and impaired treatment efficacy in acute myeloid leukemia. Transfe...
Source: IUBMB Life - March 18, 2020 Category: Research Authors: Yanning Hu, Xiaotang Ma, Zhimei Wu, Qingwei Nong, Feng Liu, Yue Wang, Min Dong Tags: RESEARCH COMMUNICATION Source Type: research

Circulating levels of C1q/TNF ‐α‐related protein 6 (CTRP6) in polycystic ovary syndrome
AbstractPolycystic ovary syndrome (PCOS) is one of the most common endocrine disorders affecting females of reproductive age. It has been associated with cardiometabolic disorders including diabetes mellitus and cardiovascular disorders, and increases the risk of developing fecundity pathologies including recurrent pregnancy loss (RPL) and infertility. C1q/tumor necrosis factor ‐α‐related protein‐6 (CTRP6) is a novel adipokine involved in glucose and lipid metabolism, host inflammation, and organogenesis. In the present study, we aimed to determine the association of serum CTRP6 levels with some components of m...
Source: IUBMB Life - March 15, 2020 Category: Research Authors: Asie Sadeghi, Reza Fadaei, Nariman Moradi, Fatima Z. Fouani, Maryam Roozbehkia, Zahra Zandieh, Soheila Ansaripour, Akram Vatannejad, Amir H. Doustimotlagh Tags: RESEARCH COMMUNICATION Source Type: research

A competition between hydrophobic and electrostatic interactions in protein –ligand systems. Binding of heterogeneously halogenated benzotriazoles by the catalytic subunit of human protein kinase CK2
AbstractA series of chlorine ‐substituted benzotriazole derivatives, representing all possible substitution patterns of halogen atoms attached to the benzotriazole benzene ring, were synthetized as potential inhibitors of human protein kinase CK2. Basic ADME parameters for the free solutes (hydrophobicity, electronic properti es) together with their binding affinity to the catalytic subunit of protein kinase CK2 were determined with reverse‐phase HPLC, spectrophotometric titration, and Thermal Shift Assay Method, respectively. The analysis of position‐dependent thermodynamic contribution of a chlorine atom attached t...
Source: IUBMB Life - March 12, 2020 Category: Research Authors: S ławomir Kasperowicz, Ewa Marzec, Agnieszka M. Maciejewska, Damian Trzybiński, Maria Bretner, Krzysztof Woźniak, Jarosław Poznański, Kinga Mieczkowska Tags: SPECIAL ISSUE Source Type: research

Issue highlights
IUBMB Life, Volume 72, Issue 4, Page 523-523, April 2020. (Source: IUBMB Life)
Source: IUBMB Life - March 11, 2020 Category: Research Tags: ISSUE HIGHLIGHTS Source Type: research

Issue Information
IUBMB Life, Volume 72, Issue 4, Page 515-522, April 2020. (Source: IUBMB Life)
Source: IUBMB Life - March 11, 2020 Category: Research Tags: ISSUE INFORMATION Source Type: research

The biofilm ‐associated bacterial infections unrelated to indwelling devices
AbstractBiofilms are microbial communities established in the self ‐produced extracellular substances that include up to 80% of associated microbial infections. During biofilm formation, bacterial cells shift from the planktonic forms to aggregated forms surrounded by an extracellular polymeric substance. The bacterial biofilm shows resistance against immune reac tions as well as antibiotics and is potentially able to cause disorders by both device‐related and nondevice‐related infections. The nondevice‐related bacterial biofilm infections include dental plaque, urinary tract infections, cystic fibrosis, otitis med...
Source: IUBMB Life - March 11, 2020 Category: Research Authors: Rasoul Mirzaei, Rokhsareh Mohammadzadeh, Mohammad Yousef Alikhani, Mohammad Shokri Moghadam, Sajad Karampoor, Sima Kazemi, Alireza Barfipoursalar, Rasoul Yousefimashouf Tags: CRITICAL REVIEW Source Type: research

The role and possible mechanism of long noncoding RNA PVT1 in modulating 3T3 ‐L1 preadipocyte proliferation and differentiation
AbstractObesity is considered as a high ‐risk susceptibility state for most metabolic disorders and is directly related to preadipocyte differentiation or adipogenesis. Long noncoding RNAs (lncRNAs) are the key factors which have regulatory functions on various critical physiological and biological processes. PVT1 was identified as an o ncogenic lncRNA which could promote angiogenesis in gastric cancer. However, the functions and molecular pathways related to PVT1 in adipogenesis had not been clarified yet. In the current study, the purpose was to identify the effects of lncRNA PVT1 on adipogenesis and the relevant molec...
Source: IUBMB Life - March 11, 2020 Category: Research Authors: Lin Zhang, Dan Zhang, Zhen ‐Ying Qin, Jing Li, Zi‐Yang Shen Tags: RESEARCH COMMUNICATION Source Type: research

Cancer stem cell enrichment is associated with enhancement of nicotinamide N ‐methyltransferase expression
In this study, a culture system was setup to enrich CSCs from bladder cancer (T24), lung cancer (A549), color ectal cancer (CaCo‐2), and osteosarcoma (MG63) cell lines, through sphere formation. Magnetic‐activated cell sorting was also used to further increase CSC enrichment. Subsequently, molecular characterization of CSC‐enriched cell populations and parental cells was carried out, by exploring the expression levels of stem markers and the enzyme nicotinamide N‐methyltransferase (NNMT). Results obtained showed a significant upregulation of stem cell markers in CSC‐enriched populations, obtained upon sphere form...
Source: IUBMB Life - March 11, 2020 Category: Research Authors: Valentina Pozzi, Eleonora Salvolini, Guendalina Lucarini, Alessia Salvucci, Roberto Campagna, Corrado Rubini, Davide Sartini, Monica Emanuelli Tags: RESEARCH COMMUNICATION Source Type: research

Elevated microRNA ‐20b‐3p and reduced thioredoxin‐interacting protein ameliorate diabetic retinopathy progression by suppressing the NLRP3 inflammasomes
AbstractOver the years, microRNA ‐20b‐3p (miR‐20b‐3p) has been found to play an essential role in human diseases; we aimed to investigate the effect of miR‐20b‐3p on the progression of diabetic retinopathy (DR). The DR rat models were established by streptozotocin injection and treated with miR‐20b‐3p mimics, silenc ed, or overexpressed thioredoxin‐interacting protein (TXNIP). Afterward, the expression of miR‐20b‐3p and TXNIP, visual function, inflammatory factors, microvascular injury, vascular permeability, cell apoptosis, and angiogenesis in rats' retinal tissues were assessed. The target relation ...
Source: IUBMB Life - March 11, 2020 Category: Research Authors: Shuai Wang, Shanshan Du, Yong Lv, Wenzhan Wang, Fengyan Zhang Tags: RESEARCH COMMUNICATION Source Type: research

The biofilm ‐associated bacterial infections unrelated to indwelling devices
AbstractBiofilms are microbial communities established in the self ‐produced extracellular substances that include up to 80% of associated microbial infections. During biofilm formation, bacterial cells shift from the planktonic forms to aggregated forms surrounded by an extracellular polymeric substance. The bacterial biofilm shows resistance against immune reac tions as well as antibiotics and is potentially able to cause disorders by both device‐related and nondevice‐related infections. The nondevice‐related bacterial biofilm infections include dental plaque, urinary tract infections, cystic fibrosis, otitis med...
Source: IUBMB Life - March 9, 2020 Category: Research Authors: Rasoul Mirzaei, Rokhsareh Mohammadzadeh, Mohammad Yousef Alikhani, Mohammad Shokri Moghadam, Sajad Karampoor, Sima Kazemi, Alireza Barfipoursalar, Rasoul Yousefimashouf Tags: CRITICAL REVIEW Source Type: research

The role and possible mechanism of long noncoding RNA PVT1 in modulating 3T3 ‐L1 preadipocyte proliferation and differentiation
AbstractObesity is considered as a high ‐risk susceptibility state for most metabolic disorders and is directly related to preadipocyte differentiation or adipogenesis. Long noncoding RNAs (lncRNAs) are the key factors which have regulatory functions on various critical physiological and biological processes. PVT1 was identified as an o ncogenic lncRNA which could promote angiogenesis in gastric cancer. However, the functions and molecular pathways related to PVT1 in adipogenesis had not been clarified yet. In the current study, the purpose was to identify the effects of lncRNA PVT1 on adipogenesis and the relevant molec...
Source: IUBMB Life - March 9, 2020 Category: Research Authors: Lin Zhang, Dan Zhang, Zhen ‐Ying Qin, Jing Li, Zi‐Yang Shen Tags: RESEARCH COMMUNICATION Source Type: research

Cancer stem cell enrichment is associated with enhancement of nicotinamide N ‐methyltransferase expression
In this study, a culture system was setup to enrich CSCs from bladder cancer (T24), lung cancer (A549), color ectal cancer (CaCo‐2), and osteosarcoma (MG63) cell lines, through sphere formation. Magnetic‐activated cell sorting was also used to further increase CSC enrichment. Subsequently, molecular characterization of CSC‐enriched cell populations and parental cells was carried out, by exploring the expression levels of stem markers and the enzyme nicotinamide N‐methyltransferase (NNMT). Results obtained showed a significant upregulation of stem cell markers in CSC‐enriched populations, obtained upon sphere form...
Source: IUBMB Life - March 9, 2020 Category: Research Authors: Valentina Pozzi, Eleonora Salvolini, Guendalina Lucarini, Alessia Salvucci, Roberto Campagna, Corrado Rubini, Davide Sartini, Monica Emanuelli Tags: RESEARCH COMMUNICATION Source Type: research

Elevated microRNA ‐20b‐3p and reduced thioredoxin‐interacting protein ameliorate diabetic retinopathy progression by suppressing the NLRP3 inflammasomes
AbstractOver the years, microRNA ‐20b‐3p (miR‐20b‐3p) has been found to play an essential role in human diseases; we aimed to investigate the effect of miR‐20b‐3p on the progression of diabetic retinopathy (DR). The DR rat models were established by streptozotocin injection and treated with miR‐20b‐3p mimics, silenc ed, or overexpressed thioredoxin‐interacting protein (TXNIP). Afterward, the expression of miR‐20b‐3p and TXNIP, visual function, inflammatory factors, microvascular injury, vascular permeability, cell apoptosis, and angiogenesis in rats' retinal tissues were assessed. The target relation ...
Source: IUBMB Life - March 9, 2020 Category: Research Authors: Shuai Wang, Shanshan Du, Yong Lv, Wenzhan Wang, Fengyan Zhang Tags: RESEARCH COMMUNICATION Source Type: research

Long noncoding RNA LINC00630 promotes radio ‐resistance by regulating BEX1 gene methylation in colorectal cancer cells
This study aimed to analyze the relation between long noncoding RNA (lncRNA) LINCE00630 and radio ‐resistance and elucidate the underlying mechanism. Relative expression of LINC00630,BEX1, and DNMT3B in colorectal cancer (CRC) cells and clinical samples was determined by real ‐time PCR. Prognosis in respect of LINC00630 expression was analyzed by Kaplan–Meier survival curve. LINC00630 andBEX1 were specifically silenced by shRNAs. Cell viability and growth were analyzed by MTT and clonogenic assays, respectively. Cell apoptosis was measure by both caspase ‐3 activity and flow cytometry. Association between EZH2 ...
Source: IUBMB Life - March 7, 2020 Category: Research Authors: Feng Liu, Weifeng Huang, Jinsheng Hong, Chuanshu Cai, Weijian Zhang, Jianrong Zhang, Zhenming Kang Tags: RESEARCH COMMUNICATION Source Type: research

Control of Akt activity and substrate phosphorylation in cells
AbstractProtein kinase B/Akt is a serine/threonine kinase that links receptors coupled to the PI3K lipid kinase to cellular anabolic pathways. Its activity in cells is controlled by reversible phosphorylation and an intramolecular lipid ‐controlled allosteric switch. In this review, I outline the current progress in understanding Akt regulatory mechanisms, define three models of Akt activation in cells, and highlight how intramolecular allosterism cooperates with cell‐autonomous mechanisms to control Akt localization and activi ty and direct it toward specific sets of substrates in cells. (Source: IUBMB Life)
Source: IUBMB Life - March 7, 2020 Category: Research Authors: Ivan Yudushkin Tags: CRITICAL REVIEW Source Type: research

HbA1c in patients with intracranial meningiomas WHO grades I and II: A preliminary study
AbstractMeningiomas are among the most common primary brain tumors. There is a growing need for novel ways of differentiating between benign (World Health Organization [WHO] grade I) and atypical (WHO grade II) meningiomas as well as for novel markers of the tumor's future behavior. A difference between glucose metabolism in atypical and benign meningiomas is well known. However, a significant correlation between the systemic metabolic status of the patient and the meningioma WHO grade has not yet been established. Our aim was to compare the WHO grades of intracranial meningiomas with the patient's HbA1c levels as a more r...
Source: IUBMB Life - March 7, 2020 Category: Research Authors: Darko Ore šković, Fadi Almahariq, Ana Majić, Patricija Sesar, Marcela Živković, Jurica Maraković, Petar Marčinković, Nina Predrijevac, Petra Vuković, Darko Chudy Tags: RESEARCH COMMUNICATION Source Type: research

miR ‐16 enhances miR‐302/367‐induced reprogramming and tumor suppression in breast cancer cells
AbstractOverexpression of either miR ‐302 or miR‐302/367 cluster induces reprogramming of cancer cells and exerts tumor‐suppressive effects by induction of mesenchymal‐to‐epithelial transition, apoptosis and a less proliferative capacity. Several reports have described miR‐16 as a tumor suppressor microRNA (miRNA). Here, we studied the impact of exogenous induction of miR‐16 in MDA‐MB‐231 and SK‐BR‐3 breast cancer cells following overexpression of miR‐302/367 cluster and investigated whether transfection of these cells by a mature miR‐16 mimic could affect the reprogramming state of the cells and ...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Moslem Hoseinbeyki, Masoumeh F. Taha, Arash Javeri Tags: RESEARCH COMMUNICATION Source Type: research

The potential role of chitosan ‐based nanoparticles as drug delivery systems in pancreatic cancer
AbstractPancreatic cancer (PC) is one of the most lethal cancers and 12th most common cancer in the world. Due to the inaccessible anatomical position of the pancreas and asymptomatic early stages of this disease, PC has a high mortality rate. Therefore, providing reliable diagnostic and therapeutic tools are the keys to increase the PC survival rate. Nanotechnology is an inchoate field of science that previously scientists' tendency to enhance the efficacy of current preventive, diagnostic, and therapeutic methods has oriented them to build a bridge between this science and medicine. In the case of PC, nanotechnology sugg...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Fatemeh Sadoughi, Mohammad Ali Mansournia, Seyyed Mehdi Mirhashemi Tags: CRITICAL REVIEW Source Type: research

MiR ‐182 inhibits kidney fibrosis by regulating transforming growth factor β1/Smad3 pathway in autosomal dominant polycystic kidney disease
AbstractThe aim of the present study was to investigate the molecular mechanism of miR ‐182 in kidney fibrosis in polycystic kidney disease (PKD). We measured the expression of miR‐182 in kidney tissue of autosomal dominant PKD. Additionally, we investigated the relationship between miR‐182 and fibrotic protein by transfecting miR‐182 mimics and miR‐182 inhibitor into polycy stic kidney cyst‐lined epithelial cells, respectively. Furthermore, we observed the interaction between transforming growth factor β1 (TGF‐β1) and miR‐182 and fibrinogen factors of cyst‐lined epithelial cells after TGF‐&be...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Liping Sun, Chaofeng Hu, Zhen Wang, Xinzhou Zhang Tags: RESEARCH COMMUNICATION Source Type: research

SWI/SNF chromatin remodeling complex and glucose metabolism are deregulated in advanced bladder cancer
AbstractBladder cancer (BC) is a frequently diagnosed malignancy affecting predominantly adult and elderly populations. It is expected that due to the longer life time, BC will become even more frequent in the future; thus in consequence, it will represent serious health problem of older society part. The treatment of advanced BC is mostly ineffective due to its very aggressive behavior. So far, no effective targeted therapy is used for BC treatment. Here, we found that BC is characterized by lower protein levels of BRM, INI1, and BAF155 main subunits of SWI/SNF chromatin remodeling complex (CRC) which is involved in globa...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Malgorzata Stachowiak, Michal Szymanski, Anna Ornoch, Iga Jancewicz, Natalia Rusetska, Alicja Chrzan, Tomasz Demkow, Janusz A. Siedlecki, Tomasz J. Sarnowski, Elzbieta Sarnowska Tags: RESEARCH COMMUNICATION Source Type: research

Certain interleukin polymorphisms might influence predisposition to lung cancer: A meta ‐analysis of 35 published studies
AbstractInterleukin polymorphisms might influence predisposition to lung cancer (LC), but the results of already published studies regarding the relationship betweeninterleukin polymorphisms and LC were still controversial and ambiguous. So the authors designed this meta ‐analysis to more precisely estimate relationship betweeninterleukin polymorphisms and LC by pooling the results of already published related studies. The authors searched Pubmed, Embase, Web of Science, and CNKI for already published studies. Thirty ‐five already published studies were pooled and analyzed in this meta‐analysis. The pooled meta‐ana...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Juwei Gao, Yinyin Ying, Jue Wang, Yiyi Cui, Weiping Zhang Tags: RESEARCH COMMUNICATION Source Type: research

Issue Information
IUBMB Life, Volume 72, Issue 3, Page 307-313, March 2020. (Source: IUBMB Life)
Source: IUBMB Life - March 4, 2020 Category: Research Tags: ISSUE INFORMATION Source Type: research

Actin remodeling, the synaptic tag and the maintenance of synaptic plasticity
AbstractActivity ‐dependent plasticity of synaptic connections is a hallmark of the mammalian brain and represents a key mechanism for rewiring neural circuits during development, experience‐dependent plasticity, and brain disorders. Cellular models of memory, such as long‐term potentiation and long‐term dep ression, share common principles to memory consolidation. As for memory, the maintenance of synaptic plasticity is dependent on the synthesis of de novo protein synthesis. The synaptic‐tagging and capture hypothesis states that the maintenance of synaptic plasticity is dependent on the interplay b etween inpu...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: J úlia Pinho, Cristina Marcut, Rosalina Fonseca Tags: CRITICAL REVIEW Source Type: research

Dysregulation of miRNAs as a signature for diagnosis and prognosis of gastric cancer and their involvement in the mechanism underlying gastric carcinogenesis and progression
AbstractGastric cancer (GC) is the second main cause of cancer ‐related mortality worldwide. The poor prognosis and survival of GC are due to diagnosis in an advanced, noncurable stage and with a restricted response to chemotherapy. GC is usually monitored in an advanced stage; therefore, the poor prognosis and lower level of survival rate with a restricted r esponse to chemotherapy can be detected. Valuable and sensible biomarkers are urgently needed to display screen patients with a high risk of GC that can complement endoscopic diagnosis. Such biomarkers will enable the efficient prediction of the therapeutic response...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Alireza Ahadi Tags: CRITICAL REVIEW Source Type: research

Development and validation of autophagy ‐related‐gene biomarker and nomogram for predicting the survival of cutaneous melanoma
AbstractAutophagy plays a critical role in cutaneous melanoma, but the prognostic research of differentially expressed autophagy ‐related genes (DEARGs) in melanoma is lacking. Therefore, autophagy‐related gene expression data of 630 melanoma patients were obtained from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas database. The DEARGs were identified by “limma” package in R software. Best survival analysi s and the least absolute shrinkage and selection operator method were performed to identify a robust 7‐DEARG signature as well as construct nomogram associated with the clinical characterist...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Qi Wan, Lin Jin, Yaru Su, Ying liu, Chaoyang Li, Zhichong Wang Tags: RESEARCH COMMUNICATION Source Type: research

Atorvastatin inhibits renal inflammatory response induced by calcium oxalate crystals via inhibiting the activation of TLR4/NF ‐κB and NLRP3 inflammasome
This study aimed to investigate the renal protective effect of atorvastatin (ATV) on the kidney inflammation induced by calcium oxalate (CaOx) crystals. A cell model of cell ‐crystal interactions and a rat model of CaOx kidney stone were established. The expressions of TLR4, NF‐κB, NLRP3, and cleaved caspase‐1 in cells and rat kidney tissues were detected using Western blot, immunohistochemical, and/or immunofluorescence. The concentrations of malondialdehyde (MD A), superoxide dismutase (SOD), reactive oxygen species (ROS) in cells, and lactic acid dehydrogenase (LDH) in the culture medium were measured. The s...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Yan Sun, Yunlong Liu, Xiaofeng Guan, Juening Kang, Xiang Wang, Quan Liu, Derong Li, Hua Xu, Zhiwei Tao, Yaoliang Deng Tags: RESEARCH COMMUNICATION Source Type: research

Thermodynamic contribution of iodine atom to the binding of heterogeneously polyhalogenated benzotriazoles by the catalytic subunit of human protein kinase CK2
AbstractA series of novel benzotriazole derivatives containing iodine atom(s) were synthesized. The binding of these compounds to the catalytic subunit of human protein kinase CK2 was evaluated using differential scanning fluorimetry. The obtained thermodynamic data were compared with those determined previously for the brominated and chlorinated benzotriazole analogues to get a deeper insight into the thermodynamic contribution of iodine substitution to the free energy of ligand binding. We have shown that iodine atom(s) attached to the benzene ring of benzotriazole enhance(s) its binding by the target protein. This effec...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Ewa Marzec, Jaros ław Poznański, Daniel Paprocki Tags: RESEARCH COMMUNICATION Source Type: research

Emerging roles of the αC‐β4 loop in protein kinase structure, function, evolution, and disease
AbstractThe faithful propagation of cellular signals in most organisms relies on the coordinated functions of a large family of protein kinases that share a conserved catalytic domain. The catalytic domain is a dynamic scaffold that undergoes large conformational changes upon activation. Most of these conformational changes, such as movement of the regulatory αC‐helix from an “out” to “in” conformation, hinge on a conserved, but understudied, loop termed the αC‐β4 loop, which mediates conserved interactions to tether flexible structural elements to the kinase core. We previously...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Wayland Yeung, Zheng Ruan, Natarajan Kannan Tags: CRITICAL REVIEW Source Type: research

Up ‐regulation of microRNA‐30b/30d cluster represses hepatocyte apoptosis in mice with fulminant hepatic failure by inhibiting CEACAM1
This study highlights that up‐regulated miR‐30b/30d attenuates the progression of FHF by targeting CEACAM1, which may be helpful to FHF treatment. (Source: IUBMB Life)
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Changpu Zhao, Zhongqin Dang, Junbo Sun, Shuaiqiang Yuan, Li Xie Tags: RESEARCH COMMUNICATION Source Type: research

LRIG3 represses cell motility by inhibiting slug via inactivating ERK signaling in human colorectal cancer
AbstractMetastasis is responsible for 90% of colorectal cancer (CRC) ‐related deaths. In the present study, we identified a novel key regulator of CRC metastasis, leucine‐rich repeats and immunoglobulin‐like domains protein 3 (LRIG3), which was significantly decreased in CRC tissues and cell lines. Downregulation of LRIG3 was attributed to copy number loss and promoter hypermethylation. Low LRIG3 expression was positively correlated with metastatic clinical features and shorter survival time. Functional experiments showed that knockout of LRIG3 markedly enhanced CRC cell migration and invasion ability, whereas reintr...
Source: IUBMB Life - March 4, 2020 Category: Research Authors: Kaixuan Zeng, Xiaoxiang Chen, Mu Xu, Xiangxiang Liu, Chenmeng Li, Xueni Xu, Bei Pan, Jian Qin, Bangshun He, Yuqin Pan, Sun Huiling, Tao Xu, Shukui Wang Tags: RESEARCH COMMUNICATION Source Type: research