CRISPR/Cas9-induced asap1a and asap1b co-knockout mutant zebrafish displayed abnormal embryonic development and impaired neutrophil migration
Gene Expr Patterns. 2023 Jun 28:119331. doi: 10.1016/j.gep.2023.119331. Online ahead of print.ABSTRACTASAP1 (Arf-GAP with SH3 domain, the ankyrin repeat and the PH domain) is the GTPase activating protein of the small G protein Arf. To understand more about the physiological functions of ASAP1 in vivo, we chose to use the zebrafish as an animal model, and analyzed the characterization of asap1 using loss-of-function studies. Here, two isoforms in zebrafish, asap1a and asap1b, were found to be homologous to human ASAP1, and the gene knockout zebrafish lines for asap1a and asap1b were established using the CRISPR/Cas9 techni...
Source: Gene Expression Patterns : GEP - June 30, 2023 Category: Genetics & Stem Cells Authors: Cui Jia Da Wen Liqing Wang Chaoqun Meng Yuhuan Wang Zhonghua Zhao Changxin Wu Source Type: research
CRISPR/Cas9-induced asap1a and asap1b co-knockout mutant zebrafish displayed abnormal embryonic development and impaired neutrophil migration
Gene Expr Patterns. 2023 Jun 28:119331. doi: 10.1016/j.gep.2023.119331. Online ahead of print.ABSTRACTASAP1 (Arf-GAP with SH3 domain, the ankyrin repeat and the PH domain) is the GTPase activating protein of the small G protein Arf. To understand more about the physiological functions of ASAP1 in vivo, we chose to use the zebrafish as an animal model, and analyzed the characterization of asap1 using loss-of-function studies. Here, two isoforms in zebrafish, asap1a and asap1b, were found to be homologous to human ASAP1, and the gene knockout zebrafish lines for asap1a and asap1b were established using the CRISPR/Cas9 techni...
Source: Gene Expression Patterns : GEP - June 30, 2023 Category: Genetics & Stem Cells Authors: Cui Jia Da Wen Liqing Wang Chaoqun Meng Yuhuan Wang Zhonghua Zhao Changxin Wu Source Type: research
CRISPR/Cas9-induced asap1a and asap1b co-knockout mutant zebrafish displayed abnormal embryonic development and impaired neutrophil migration
Gene Expr Patterns. 2023 Jun 28:119331. doi: 10.1016/j.gep.2023.119331. Online ahead of print.ABSTRACTASAP1 (Arf-GAP with SH3 domain, the ankyrin repeat and the PH domain) is the GTPase activating protein of the small G protein Arf. To understand more about the physiological functions of ASAP1 in vivo, we chose to use the zebrafish as an animal model, and analyzed the characterization of asap1 using loss-of-function studies. Here, two isoforms in zebrafish, asap1a and asap1b, were found to be homologous to human ASAP1, and the gene knockout zebrafish lines for asap1a and asap1b were established using the CRISPR/Cas9 techni...
Source: Gene Expression Patterns : GEP - June 30, 2023 Category: Genetics & Stem Cells Authors: Cui Jia Da Wen Liqing Wang Chaoqun Meng Yuhuan Wang Zhonghua Zhao Changxin Wu Source Type: research
The role of mip in the development of lens in zebrafish
In this study, a zebrafish model with the mipb mutant was produced. The expression of mipb mRNA and protein was dramatically reduced in the mutant. Immunological analysis reveals that loss function of mip leads to the diffuse distribution of ZL-1 in the mutant lens. Furthermore, in situ hybridization reveals that mip knockout results in a decrease in the transcripts of beaded filament structural protein 2 (Bfsp2) in the lens. Histology study shows that lens fibers in the mutants are less uniform in shape and the fiber arrangement is disrupted. The presented data provides evidence for the essential role of mipb in the devel...
Source: Gene Expression Patterns : GEP - June 27, 2023 Category: Genetics & Stem Cells Authors: Mingyan He Guangkai Zhou Qinghong Lin Nan Zhou Source Type: research
The role of mip in the development of lens in zebrafish
In this study, a zebrafish model with the mipb mutant was produced. The expression of mipb mRNA and protein was dramatically reduced in the mutant. Immunological analysis reveals that loss function of mip leads to the diffuse distribution of ZL-1 in the mutant lens. Furthermore, in situ hybridization reveals that mip knockout results in a decrease in the transcripts of beaded filament structural protein 2 (Bfsp2) in the lens. Histology study shows that lens fibers in the mutants are less uniform in shape and the fiber arrangement is disrupted. The presented data provides evidence for the essential role of mipb in the devel...
Source: Gene Expression Patterns : GEP - June 27, 2023 Category: Genetics & Stem Cells Authors: Mingyan He Guangkai Zhou Qinghong Lin Nan Zhou Source Type: research
The role of mip in the development of lens in zebrafish
In this study, a zebrafish model with the mipb mutant was produced. The expression of mipb mRNA and protein was dramatically reduced in the mutant. Immunological analysis reveals that loss function of mip leads to the diffuse distribution of ZL-1 in the mutant lens. Furthermore, in situ hybridization reveals that mip knockout results in a decrease in the transcripts of beaded filament structural protein 2 (Bfsp2) in the lens. Histology study shows that lens fibers in the mutants are less uniform in shape and the fiber arrangement is disrupted. The presented data provides evidence for the essential role of mipb in the devel...
Source: Gene Expression Patterns : GEP - June 27, 2023 Category: Genetics & Stem Cells Authors: Mingyan He Guangkai Zhou Qinghong Lin Nan Zhou Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
hnRNPL expression dynamics in the embryo and placenta
Gene Expr Patterns. 2023 Jun;48:119319. doi: 10.1016/j.gep.2023.119319. Epub 2023 May 4.ABSTRACTHeterogeneous nuclear ribonucleoprotein L (hnRNPL) is a conserved RNA binding protein (RBP) that plays an important role in the alternative splicing of gene transcripts, and thus in the generation of specific protein isoforms. Global deficiency in hnRNPL in mice results in preimplantation embryonic lethality at embryonic day (E) 3.5. To begin to understand the contribution of hnRNPL-regulated pathways in the normal development of the embryo and placenta, we determined hnRNPL expression profile and subcellular localization throug...
Source: Gene Expression Patterns : GEP - May 6, 2023 Category: Genetics & Stem Cells Authors: Vineetha Mathew Ariel Mei Hamida Giwa Agnes Cheong Ashmita Chander Aaron Zou Robert M Blanton Olga Kashpur Wei Cui Donna Slonim Taysir Mahmoud Perrie O'Tierney-Ginn Jesse Mager Isabelle Draper Mary C Wallingford Source Type: research
