Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Pluripotency and embryonic lineage genes expression in the presence of small molecule inhibitors of FGF, TGF β and GSK3 during pre-implantation development of goat embryos
In this study, we investigated embryo development and gene expression of the core pluripotency triad (OCT4, NANOG, SOX2) and cell lineage commitment markers (REX1, CDX2, GATA4) in the presence of three small molecules and their combination [PD0325901 (FGF inhibitor), SB431542 (TGFβ inhibitor), and CHIR99021 (GSK3B inhibitor)] from day 2-7 post-insemination in goat. Significant reduction in rate of blastocyst formation was observed when SB was used along with PD or CHIR and their three combinations had more sever effect. SB and CHIR decreased the expression of SOX2 while increasing the GATA4 expression. PD decrease the rel...
Source: Gene Expression Patterns : GEP - September 7, 2023 Category: Genetics & Stem Cells Authors: Mehdi Hajian Shiva Rouhollahi Varnosfaderani Farnoosh Jafarpour Nima Tanhaei Vash Mohammad Hossein Nasr-Esfahani Source Type: research
Editorial Note
Gene Expr Patterns. 2023 Sep 2:119335. doi: 10.1016/j.gep.2023.119335. Online ahead of print.NO ABSTRACTPMID:37666315 | DOI:10.1016/j.gep.2023.119335 (Source: Gene Expression Patterns : GEP)
Source: Gene Expression Patterns : GEP - September 4, 2023 Category: Genetics & Stem Cells Source Type: research
Editorial Note
Gene Expr Patterns. 2023 Sep 2:119335. doi: 10.1016/j.gep.2023.119335. Online ahead of print.NO ABSTRACTPMID:37666315 | DOI:10.1016/j.gep.2023.119335 (Source: Gene Expression Patterns : GEP)
Source: Gene Expression Patterns : GEP - September 4, 2023 Category: Genetics & Stem Cells Source Type: research
Editorial Note
Gene Expr Patterns. 2023 Sep 2:119335. doi: 10.1016/j.gep.2023.119335. Online ahead of print.NO ABSTRACTPMID:37666315 | DOI:10.1016/j.gep.2023.119335 (Source: Gene Expression Patterns : GEP)
Source: Gene Expression Patterns : GEP - September 4, 2023 Category: Genetics & Stem Cells Source Type: research
Editorial Note
Gene Expr Patterns. 2023 Sep 2:119335. doi: 10.1016/j.gep.2023.119335. Online ahead of print.NO ABSTRACTPMID:37666315 | DOI:10.1016/j.gep.2023.119335 (Source: Gene Expression Patterns : GEP)
Source: Gene Expression Patterns : GEP - September 4, 2023 Category: Genetics & Stem Cells Source Type: research
Spatiotemporal expression patterns of R-spondins and their receptors, Lgrs, in the developing mouse telencephalon
In this study, we examined the detailed spatiotemporal expression patterns of Rspo1-4 and Lgr4-6 in the embryonic and postnatal telencephalon to elucidate their functions. In the embryonic day (E) 10.5-14.5 telencephalon, Rspo1-3 were prominently expressed in the cortical hem. Among their receptors, Lgr4 was observed in the ventral telencephalon, and Lgr6 was highly expressed throughout the telencephalon at the same stages. This suggests that Rspo1-3 and Lgr4 initially regulate telencephalic development in restricted regions, whereas Lgr6 functions broadly. From the late embryonic stage, the expression areas of Rspo1-3 and...
Source: Gene Expression Patterns : GEP - August 31, 2023 Category: Genetics & Stem Cells Authors: Keisuke Watanabe Masao Horie Manabu Hayatsu Yoshikazu Mikami Noboru Sato Source Type: research
