COVID-19: why not learn from the past?
(Source: Frontiers of Medicine)
Source: Frontiers of Medicine - August 31, 2021 Category: General Medicine Source Type: research
Hybrid deep learning model for risk prediction of fracture in patients with diabetes and osteoporosis
AbstractThe fracture risk of patients with diabetes is higher than those of patients without diabetes due to hyperglycemia, usage of diabetes drugs, changes in insulin levels, and excretion, and this risk begins as early as adolescence. Many factors including demographic data (such as age, height, weight, and gender), medical history (such as smoking, drinking, and menopause), and examination (such as bone mineral density, blood routine, and urine routine) may be related to bone metabolism in patients with diabetes. However, most of the existing methods are qualitative assessments and do not consider the interactions of th...
Source: Frontiers of Medicine - August 26, 2021 Category: General Medicine Source Type: research
Case report of neurofibromatosis type 1 combined with primary ciliary dyskinesia
AbstractNeurofibromatosis (NF) is a genetic disease in which the lungs are rarely involved. However, in NF cases with lung involvement, chest computed tomography may show bilateral basal reticulations, apical bullae, and cysts without bronchiectasis. Herein, we report a patient diagnosed with NF on the basis of the results of genetic testing who presented with early-onset wet cough and bronchiectasis. Considering the differential diagnosis of bronchiectasis combined with his early-onset wet cough, sinusitis, and sperm quality decline, we considered the possibility of primary ciliary dyskinesia (PCD). Further electron micro...
Source: Frontiers of Medicine - August 25, 2021 Category: General Medicine Source Type: research
Clinical characteristics and risk factors of COVID-19 patients with chronic hepatitis B: a multi-center retrospective cohort study
In conclusion, COVID-19 patients with CHB were more likely to develop into severe illness and die. The risk factors that we identified may be helpful for early clinical surveillance of critical progression. (Source: Frontiers of Medicine)
Source: Frontiers of Medicine - August 13, 2021 Category: General Medicine Source Type: research
Clinical significance of CD34+CD117dim/CD34+CD117bri myeloblast-associated gene expression in t(8;21) acute myeloid leukemia
In this study, 85 patients with t(8;21) AML were enrolled. The mRNA expression levels of CD34+CD117dim-associated genes (LGALS1, EMP3, andCRIP1) and CD34+CD117bri-associated genes (TRH, PLAC8, andIGLL1) were measured using quantitative reverse transcription PCR. Associations between gene expression and clinical outcomes were determined using Cox regression models. Results showed that patients with highLGALS1, EMP3, orCRIP1 expression had significantly inferior overall survival (OS), whereas those with highTRH orPLAC8 expression showed relatively favorable prognosis. Univariate analysis revealed that CD19, CD34+CD117dim pro...
Source: Frontiers of Medicine - August 1, 2021 Category: General Medicine Source Type: research
IRF4 and IRF8 expression are associated with clinical phenotype and clinico-hematological response to hydroxyurea in essential thrombocythemia
In this study, we evaluated the expression of IRF4 and IRF8 and the JAK2V617F mutant allele burden in patients with MPNs. Patients with decreased IRF4 expression were correlated with a more developed MPN phenotype in myelofibrosis (MF) and secondary AML (sAML) transformed from MPNs versus essential thrombocythemia (ET). Negative correlations between the JAK2V617F allele burden and the expression of IRF8 (P< 0.05) and IRF4 (P< 0.001) and between white blood cell (WBC) count and IRF4 expression (P< 0.05) were found in ET patients. IRF8 expression was negatively correlated with the JAK2V617F allele burden (P< 0.05...
Source: Frontiers of Medicine - July 31, 2021 Category: General Medicine Source Type: research
Potential of electron transfer and its application in dictating routes of biochemical processes associated with metabolic reprogramming
AbstractMetabolic reprogramming, such as abnormal utilization of glucose, addiction to glutamine, and increasedde-novo lipid synthesis, extensively occurs in proliferating cancer cells, but the underneath rationale has remained to be elucidated. Based on the concept of the degree of reduction of a compound, we have recently proposed a calculation termed as potential of electron transfer (PET), which is used to characterize the degree of electron redistribution coupled with metabolic transformations. When this calculation is combined with the assumed model of electron balance in a cellular context, the enforced selective re...
Source: Frontiers of Medicine - July 24, 2021 Category: General Medicine Source Type: research
Clinical factors associated with composition of lung microbiota and important taxa predicting clinical prognosis in patients with severe community-acquired pneumonia
AbstractFew studies have described the key features and prognostic roles of lung microbiota in patients with severe community-acquired pneumonia (SCAP). We prospectively enrolled consecutive SCAP patients admitted to ICU. Bronchoscopy was performed at bedside within 48 h of ICU admission, and 16S rRNA gene sequencing was applied to the collected bronchoalveolar lavage fluid. The primary outcome was clinical improvements defined as a decrease of 2 categories and above on a 7-category ordinal scale within 14 days following bronchoscopy. Sixty-seven patients were included. Multivariable permutational multivariate analysis of ...
Source: Frontiers of Medicine - July 24, 2021 Category: General Medicine Source Type: research
Novel mutation c.1210-3C & gt; G in cis with a poly-T tract of 5T affects CFTR mRNA splicing in a Chinese patient with cystic fibrosis
AbstractCystic fibrosis (CF) is a rare autosomal recessive disease with only one pathogenic gene cystic fibrosis transmembrane conductance regulator (CFTR). To identify the potential pathogenic mutations in a Chinese patient with CF, we conducted Sanger sequencing on the genomic DNA of the patient and his parents and detected all 27 coding exons ofCFTR and their flanking intronic regions. The patient is a compound heterozygote of c.2909G> A, p.Gly970Asp in exon 18 and c.1210-3C> G incis with a poly-T of 5T (T5) sequence, 3 bp upstream in intron 9. The splicing effect of c.1210-3C> G was verified via minigene assay...
Source: Frontiers of Medicine - July 24, 2021 Category: General Medicine Source Type: research
Erratum to: Midline2 is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro and in vivo
(Source: Frontiers of Medicine)
Source: Frontiers of Medicine - July 19, 2021 Category: General Medicine Source Type: research
Second unmanipulated allogeneic transplantation could be used as a salvage option for patients with relapsed acute leukemia post-chemotherapy plus modified donor lymphocyte infusion
AbstractRelapse is the main problem after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The outcome of a second allo-HSCT (HSCT2) for relapse post-HSCT has shown promising results in some previous studies. However, little is known about the efficacy of HSCT2 in patients with relapsed/refractory acute leukemia (AL) post-chemotherapy plus modified donor lymphocyte infusion (post-Chemo + m-DLI) after the first allo-HSCT (HSCT1). Therefore, we retrospectively analyzed the efficacy of HSCT2 in 28 patients with relapsed/refractory AL post-Chemo + m-DLI in our center. With a median follow-up of 918 (457 –1732)...
Source: Frontiers of Medicine - July 19, 2021 Category: General Medicine Source Type: research
Quantitative proteomics revealed extensive microenvironmental changes after stem cell transplantation in ischemic stroke
This study aimed to investigate the microenvironmental changes in the subacute and chronic phases of ischemic stroke after stem cell transplantation. Herein, induced pluripotent stem cells (iPSCs) and neural stem cells (NSCs) were transplanted into the ischemic brain established by middle cerebral artery occlusion surgery. Positron emission tomography imaging and neurological tests were applied to evaluate the metabolic and neurofunctional alterations of rats transplanted with stem cells. Quantitative proteomics was employed to investigate the protein expression profiles in iPSCs-transplanted brain in the subacute and chro...
Source: Frontiers of Medicine - July 9, 2021 Category: General Medicine Source Type: research
Proteomics study of Mycoplasma pneumoniae pneumonia reveals the Fc fragment of the IgG-binding protein as a serum biomarker and implicates potential therapeutic targets
In this study, serum samples were collected from patients with general MPP (GMPP) and SMPP to conduct proteomics profiling. The Fc fragment of the IgG-binding protein (FCGBP) was identified as the most promising indicator of SMPP. Biological enrichment analysis indicated uncontrolled inflammation in SMPP. ELISA results proved that the FCGBP level in patients with SMPP was substantially higher than that in patients with GMPP. Furthermore, the FCGBP levels showed a decreasing trend in patients with GMPP but the opposite trend in patients with SMPP during disease progression. Connectivity map analyses identified 25 possible t...
Source: Frontiers of Medicine - July 9, 2021 Category: General Medicine Source Type: research
Renin-angiotensin system inhibitor is associated with the reduced risk of all-cause mortality in COVID-19 among patients with/without hypertension
AbstractConsecutively hospitalized patients with confirmed coronavirus disease 2019 (COVID-19) in Wuhan, China were retrospectively enrolled from January 2020 to March 2020 to investigate the association between the use of renin –angiotensin system inhibitor (RAS-I) and the outcome of this disease. Associations between the use of RAS-I (angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor blocker (ARB)), ACEI, and ARB and in-hospital mortality were analyzed using multivariate Cox proportional hazards reg ression models in overall and subgroup of hypertension status. A total of 2771 patients with COVID-1...
Source: Frontiers of Medicine - July 9, 2021 Category: General Medicine Source Type: research
Particulate matter 2.5 triggers airway inflammation and bronchial hyperresponsiveness in mice by activating the SIRT2-p65 pathway
AbstractExposure to particulate matter 2.5 (PM2.5) potentially triggers airway inflammation by activating nuclear factor- κB (NF-κB). Sirtuin 2 (SIRT2) is a key modulator in inflammation. However, the function and specific mechanisms of SIRT2 in PM2.5-induced airway inflammation are largely understudied. Therefore, this work investigated the mechanisms of SIRT2 in regulating the phosphorylation and acetylation of p65 influenced by PM2.5-induced airway inflammation and bronchial hyperresponsiveness. Results revealed that PM2.5 exposure lowered the expression and activity of SIRT2 in bronchial tissues. Subsequently, SIRT2 ...
Source: Frontiers of Medicine - June 28, 2021 Category: General Medicine Source Type: research
