Discovery of the mechanisms of acupuncture in the treatment of migraine based on functional magnetic resonance imaging and omics
This study aims to provide further clinical evidence for the anti-migraine effects of acupuncture and explore the mechanism involved. A randomized controlled trial was performed among 10 normal controls and 38 migraineurs. The migraineurs were divided into blank control, sham acupuncture, and acupuncture groups. Patients were subjected to two courses of treatment, and each treatment lasted for 5 days, with an interval of 1 day between the two courses. The effectiveness of treatment was evaluated using pain questionnaire. The functional magnetic resonance imaging (fMRI) data were analyzed for investigating brain changes ind...
Source: Frontiers of Medicine - June 30, 2023 Category: General Medicine Source Type: research
Effectiveness of inactivated COVID-19 vaccine against the severity of Omicron variant
(Source: Frontiers of Medicine)
Source: Frontiers of Medicine - June 27, 2023 Category: General Medicine Source Type: research
Dynein axonemal heavy chain 10 deficiency causes primary ciliary dyskinesia in humans and mice
AbstractPrimary ciliary dyskinesia (PCD) is a congenital, motile ciliopathy with pleiotropic symptoms. Although nearly 50 causative genes have been identified, they only account for approximately 70% of definitive PCD cases. Dynein axonemal heavy chain 10 (DNAH10) encodes a subunit of the inner arm dynein heavy chain in motile cilia and sperm flagella. Based on the common axoneme structure of motile cilia and sperm flagella,DNAH10 variants are likely to cause PCD. Using exome sequencing, we identified a novelDNAH10 homozygous variant (c.589C> T, p.R197W) in a patient with PCD from a consanguineous family. The patient ma...
Source: Frontiers of Medicine - June 13, 2023 Category: General Medicine Source Type: research
Distinct mononuclear diploid cardiac subpopulation with minimal cell –cell communications persists in embryonic and adult mammalian heart
In conclusion, a novel non-pMNDCM subpopulation with minimal cell –cell communications was unveiled, highlighting the importance of microenvironment contribution to CM fate during maturation. These findings could improve the understanding of MNDCM heterogeneity and cardiac development, thus providing new clues for approaches to effective cardiac regeneration. (Source: Frontiers of Medicine)
Source: Frontiers of Medicine - June 9, 2023 Category: General Medicine Source Type: research
Hyperosmolarity promotes macrophage pyroptosis by driving the glycolytic reprogramming of corneal epithelial cells in dry eye disease
In this study, the mechanisms involving the hyperosmolar microenvironment, glycolysis mediating metabolic reprogramming, and pyroptosis were explored clinically,in vitro, andin vivo. Data from DED clinical samples indicated that the expression of glycolysis and pyroptosis-related genes, includingPKM2 andGSDMD, was significantly upregulated and that the secretion of IL-1 β significantly increased.In vitro, the indirect coculture of macrophages derived from THP-1 and human corneal epithelial cells (HCECs) was used to discuss the interaction among cells. The hyperosmolar environment was found to greatly induce HCECs ’ meta...
Source: Frontiers of Medicine - June 2, 2023 Category: General Medicine Source Type: research
Progress and challenges in RET-targeted cancer therapy
AbstractThe rearranged during transfection (RET) is a receptor protein tyrosine kinase. Oncogenic RET fusions or mutations are found most often in non-small cell lung cancer (NSCLC) and in thyroid cancer, but also increasingly in various types of cancers at low rates. In the last few years, two potent and selective RET protein tyrosine kinase inhibitors (TKIs), pralsetinib (BLU-667) and selpercatinib (LOXO-292, LY3527723) were developed and received regulatory approval. Although pralsetinib and selpercatinib gave high overall response rates (ORRs),< 10% of patients achieved a complete response (CR). The RET TKI-tolerate...
Source: Frontiers of Medicine - May 3, 2023 Category: General Medicine Source Type: research
ACSL5, a prognostic factor in acute myeloid leukemia, modulates the activity of Wnt/ β-catenin signaling by palmitoylation modification
AbstractAcyl-CoA synthetase long chain family member 5 (ACSL5), is a member of the acyl-CoA synthetases (ACSs) family that activates long chain fatty acids by catalyzing the synthesis of fatty acyl-CoAs. The dysregulation of ACSL5 has been reported in some cancers, such as glioma and colon cancers. However, little is known about the role of ACSL5 in acute myeloid leukemia (AML). We found that the expression of ACSL5 was higher in bone marrow cells from AML patients compared with that from healthy donors. ACSL5 level could serve as an independent prognostic predictor of the overall survival of AML patients. In AML cells, th...
Source: Frontiers of Medicine - May 3, 2023 Category: General Medicine Source Type: research
Zooming in and out of ferroptosis in human disease
AbstractFerroptosis is defined as an iron-dependent regulated form of cell death driven by lipid peroxidation. In the past decade, it has been implicated in the pathogenesis of various diseases that together involve almost every organ of the body, including various cancers, neurodegenerative diseases, cardiovascular diseases, lung diseases, liver diseases, kidney diseases, endocrine metabolic diseases, iron-overload-related diseases, orthopedic diseases and autoimmune diseases. Understanding the underlying molecular mechanisms of ferroptosis and its regulatory pathways could provide additional strategies for the management...
Source: Frontiers of Medicine - May 1, 2023 Category: General Medicine Source Type: research
Dihydroartemisinin increased the abundance of Akkermansia muciniphila by YAP1 depression that sensitizes hepatocellular carcinoma to anti-PD-1 immunotherapy
AbstractThe effect of anti-programmed cell death 1 (anti-PD-1) immunotherapy is limited in patients with hepatocellular carcinoma (HCC). Yes-associated protein 1 (YAP1) expression increased in liver tumor cells in early HCC, andAkkermansia muciniphila abundance decreased in the colon. The response to anti-PD-1 treatment is associated withA. muciniphila abundance in many tumors. However, the interaction betweenA. muciniphila abundance and YAP1 expression remains unclear in HCC. Here, anti-PD-1 treatment decreasedA. muciniphila abundance in the colon, but increased YAP1 expression in the tumor cells by mice with liver tumors...
Source: Frontiers of Medicine - May 1, 2023 Category: General Medicine Source Type: research
Machine learning modeling identifies hypertrophic cardiomyopathy subtypes with genetic signature
AbstractPrevious studies have revealed that patients with hypertrophic cardiomyopathy (HCM) exhibit differences in symptom severity and prognosis, indicating potential HCM subtypes among these patients. Here, 793 patients with HCM were recruited at an average follow-up of 32.78 ± 27.58 months to identify potential HCM subtypes by performing consensus clustering on the basis of their echocardiography features. Furthermore, we proposed a systematic method for illustrating the relationship between the phenotype and genotype of each HCM subtype by using machine learning model ing and interactome network detection techniques b...
Source: Frontiers of Medicine - May 1, 2023 Category: General Medicine Source Type: research
Rare tumors: a blue ocean of investigation
AbstractAdvances in novel drugs, therapies, and genetic techniques have revolutionized the diagnosis and treatment of cancers, substantially improving cancer patients ’ prognosis. Although rare tumors account for a non-negligible number, the practice of precision medicine and development of novel therapies are largely hampered by many obstacles. Their low incidence and drastic regional disparities result in the difficulty of informative evidence-based diagnosis and subtyping. Sample exhaustion due to difficulty in diagnosis also leads to a lack of recommended therapeutic strategies in clinical guidelines, insufficient bi...
Source: Frontiers of Medicine - April 26, 2023 Category: General Medicine Source Type: research
FERM domain-containing protein FRMD6 activates the mTOR signaling pathway and promotes lung cancer progression
AbstractFRMD6, a member of the 4.1 ezrin-radixin-moesin domain-containing protein family, has been reported to inhibit tumor progression in multiple cancers. Here, we demonstrate the involvement of FRMD6 in lung cancer progression. We find that FRMD6 is overexpressed in lung cancer tissues relative to in normal lung tissues. In addition, the enhanced expression of FRMD6 is associated with poor outcomes in patients with lung squamous cell carcinoma (n = 75,P = 0.0054) and lung adenocarcinoma (n = 94,P = 0.0330). Cell migration and proliferationin vitro and tumor formationin vivo are promoted by FRMD6 but are suppressed by t...
Source: Frontiers of Medicine - April 15, 2023 Category: General Medicine Source Type: research
Immunosuppressive tumor microenvironment contributes to tumor progression in diffuse large B-cell lymphoma upon anti-CD19 chimeric antigen receptor T therapy
AbstractAnti-CD19 chimeric antigen receptor (CAR)-T cell therapy has achieved 40% –50% long-term complete response in relapsed or refractory diffuse large B-cell lymphoma (DLBCL) patients. However, the underlying mechanism of alterations in the tumor microenvironments resulting in CAR-T cell therapy failure needs further investigation. A multi-center phase I/II trial of anti-CD 19 CD28z CAR-T (FKC876, ChiCTR1800019661) was conducted. Among 22 evaluable DLBCL patients, seven achieved complete remission, 10 experienced partial remissions, while four had stable disease by day 29. Single-cell RNA sequencing results were obta...
Source: Frontiers of Medicine - April 15, 2023 Category: General Medicine Source Type: research
Evaluation of ICUs and weight of quality control indicators: an exploratory study based on Chinese ICU quality data from 2015 to 2020
This study aimed to explore key quality control factors that affected the prognosis of intensive care unit (ICU) patients in Chinese mainland over six years (2015 –2020). The data for this study were from 31 provincial and municipal hospitals (3425 hospital ICUs) and included 2 110 685 ICU patients, for a total of 27 607 376 ICU hospitalization days. We found that 15 initially established quality control indicators were good predictors of patient prognosis, including percentage of ICU patients out of all inpatients (%), percentage of ICU bed occupancy of total inpatient bed occupancy (%), percentage of all ICU inpatients...
Source: Frontiers of Medicine - April 15, 2023 Category: General Medicine Source Type: research
Human menstrual blood-derived stem cells alleviate autoimmune hepatitis via JNK/MAPK signaling pathway in vivo and in vitro
AbstractAutoimmune hepatitis (AIH) is a severe globally distributed liver disease that could occur at any age. Human menstrual blood-derived stem cells (MenSCs) have shown therapeutic effect in acute lung injury and liver failure. However, their role in the curative effect of AIH remains unclear. Here, a classic AIH mouse model was constructed through intravenous injection with concanavalin A (Con A). MenSCs were intravenously injected while Con A injection in the treatment groups. The results showed that the mortality by Con A injection was significantly decreased by MenSCs treatment and liver function tests and histologi...
Source: Frontiers of Medicine - April 3, 2023 Category: General Medicine Source Type: research
