Spatio-temporal dynamics of the DNA glycosylase OGG1 in finding and processing 8-oxoguanine
DNA Repair (Amst). 2023 Jul 31;129:103550. doi: 10.1016/j.dnarep.2023.103550. Online ahead of print.ABSTRACTOGG1 is the DNA glycosylase responsible for the removal of the oxidative lesion 8-oxoguanine (8-oxoG) from DNA. The recognition of this lesion by OGG1 is a complex process that involves scanning the DNA for the presence of 8-oxoG, followed by recognition and lesion removal. Structural data have shown that OGG1 evolves through different stages of conformation onto the DNA, corresponding to elementary steps of the 8-oxoG recognition and extrusion from the double helix. Single-molecule studies of OGG1 on naked DNA have ...
Source: DNA Repair - August 5, 2023 Category: Genetics & Stem Cells Authors: Luana Cintori Anne-Marie Di Guilmi Yvan Canitrot Sebastien Huet Anna Campalans Source Type: research
Spatio-temporal dynamics of the DNA glycosylase OGG1 in finding and processing 8-oxoguanine
DNA Repair (Amst). 2023 Jul 31;129:103550. doi: 10.1016/j.dnarep.2023.103550. Online ahead of print.ABSTRACTOGG1 is the DNA glycosylase responsible for the removal of the oxidative lesion 8-oxoguanine (8-oxoG) from DNA. The recognition of this lesion by OGG1 is a complex process that involves scanning the DNA for the presence of 8-oxoG, followed by recognition and lesion removal. Structural data have shown that OGG1 evolves through different stages of conformation onto the DNA, corresponding to elementary steps of the 8-oxoG recognition and extrusion from the double helix. Single-molecule studies of OGG1 on naked DNA have ...
Source: DNA Repair - August 5, 2023 Category: Genetics & Stem Cells Authors: Luana Cintori Anne-Marie Di Guilmi Yvan Canitrot Sebastien Huet Anna Campalans Source Type: research
Spatio-temporal dynamics of the DNA glycosylase OGG1 in finding and processing 8-oxoguanine
DNA Repair (Amst). 2023 Jul 31;129:103550. doi: 10.1016/j.dnarep.2023.103550. Online ahead of print.ABSTRACTOGG1 is the DNA glycosylase responsible for the removal of the oxidative lesion 8-oxoguanine (8-oxoG) from DNA. The recognition of this lesion by OGG1 is a complex process that involves scanning the DNA for the presence of 8-oxoG, followed by recognition and lesion removal. Structural data have shown that OGG1 evolves through different stages of conformation onto the DNA, corresponding to elementary steps of the 8-oxoG recognition and extrusion from the double helix. Single-molecule studies of OGG1 on naked DNA have ...
Source: DNA Repair - August 5, 2023 Category: Genetics & Stem Cells Authors: Luana Cintori Anne-Marie Di Guilmi Yvan Canitrot Sebastien Huet Anna Campalans Source Type: research
The multifaceted functions of homologous recombination in dealing with replication-associated DNA damages
DNA Repair (Amst). 2023 Aug 1;129:103548. doi: 10.1016/j.dnarep.2023.103548. Online ahead of print.ABSTRACTThe perturbation of DNA replication, a phenomena termed "replication stress", is a driving force of genome instability and a hallmark of cancer cells. Among the DNA repair mechanisms that contribute to tolerating replication stress, the homologous recombination pathway is central to the alteration of replication fork progression. In many organisms, defects in the homologous recombination machinery result in increased cell sensitivity to replication-blocking agents and a higher risk of cancer in humans. Moreover, the s...
Source: DNA Repair - August 4, 2023 Category: Genetics & Stem Cells Authors: Shrena Chakraborty Kamila Schirmeisen Sarah Ae Lambert Source Type: research
The multifaceted functions of homologous recombination in dealing with replication-associated DNA damages
DNA Repair (Amst). 2023 Aug 1;129:103548. doi: 10.1016/j.dnarep.2023.103548. Online ahead of print.ABSTRACTThe perturbation of DNA replication, a phenomena termed "replication stress", is a driving force of genome instability and a hallmark of cancer cells. Among the DNA repair mechanisms that contribute to tolerating replication stress, the homologous recombination pathway is central to the alteration of replication fork progression. In many organisms, defects in the homologous recombination machinery result in increased cell sensitivity to replication-blocking agents and a higher risk of cancer in humans. Moreover, the s...
Source: DNA Repair - August 4, 2023 Category: Genetics & Stem Cells Authors: Shrena Chakraborty Kamila Schirmeisen Sarah Ae Lambert Source Type: research
The analysis of protein recruitment to laser microirradiation-induced DNA damage in live cells: Best practices for data analysis
DNA Repair (Amst). 2023 Jul 27;129:103545. doi: 10.1016/j.dnarep.2023.103545. Online ahead of print.ABSTRACTLaser microirradiation coupled with live-cell fluorescence microscopy is a powerful technique that has been used widely in studying the recruitment and retention of proteins at sites of DNA damage. Results obtained from this technique can be found in published works by both seasoned and infrequent users of microscopy. However, like many other microscopy-based techniques, the presentation of data from laser microirradiation experiments is inconsistent; papers report a wide assortment of analytic techniques, not all of...
Source: DNA Repair - July 31, 2023 Category: Genetics & Stem Cells Authors: Ryan M Bianchini Ebba U Kurz Source Type: research
The analysis of protein recruitment to laser microirradiation-induced DNA damage in live cells: Best practices for data analysis
DNA Repair (Amst). 2023 Jul 27;129:103545. doi: 10.1016/j.dnarep.2023.103545. Online ahead of print.ABSTRACTLaser microirradiation coupled with live-cell fluorescence microscopy is a powerful technique that has been used widely in studying the recruitment and retention of proteins at sites of DNA damage. Results obtained from this technique can be found in published works by both seasoned and infrequent users of microscopy. However, like many other microscopy-based techniques, the presentation of data from laser microirradiation experiments is inconsistent; papers report a wide assortment of analytic techniques, not all of...
Source: DNA Repair - July 31, 2023 Category: Genetics & Stem Cells Authors: Ryan M Bianchini Ebba U Kurz Source Type: research
The analysis of protein recruitment to laser microirradiation-induced DNA damage in live cells: Best practices for data analysis
DNA Repair (Amst). 2023 Jul 27;129:103545. doi: 10.1016/j.dnarep.2023.103545. Online ahead of print.ABSTRACTLaser microirradiation coupled with live-cell fluorescence microscopy is a powerful technique that has been used widely in studying the recruitment and retention of proteins at sites of DNA damage. Results obtained from this technique can be found in published works by both seasoned and infrequent users of microscopy. However, like many other microscopy-based techniques, the presentation of data from laser microirradiation experiments is inconsistent; papers report a wide assortment of analytic techniques, not all of...
Source: DNA Repair - July 31, 2023 Category: Genetics & Stem Cells Authors: Ryan M Bianchini Ebba U Kurz Source Type: research
The analysis of protein recruitment to laser microirradiation-induced DNA damage in live cells: Best practices for data analysis
DNA Repair (Amst). 2023 Jul 27;129:103545. doi: 10.1016/j.dnarep.2023.103545. Online ahead of print.ABSTRACTLaser microirradiation coupled with live-cell fluorescence microscopy is a powerful technique that has been used widely in studying the recruitment and retention of proteins at sites of DNA damage. Results obtained from this technique can be found in published works by both seasoned and infrequent users of microscopy. However, like many other microscopy-based techniques, the presentation of data from laser microirradiation experiments is inconsistent; papers report a wide assortment of analytic techniques, not all of...
Source: DNA Repair - July 31, 2023 Category: Genetics & Stem Cells Authors: Ryan M Bianchini Ebba U Kurz Source Type: research
Functional analyses of single nucleotide polymorphic variants of the DNA glycosylase NEIL1 present in sub-Saharan African populations
This study provides insight regarding the potential increased risk for early-onset HCC in human populations carrying the NEIL1 I182M variant.PMID:37517321 | DOI:10.1016/j.dnarep.2023.103544 (Source: DNA Repair)
Source: DNA Repair - July 30, 2023 Category: Genetics & Stem Cells Authors: Jamie T Zuckerman Irina G Minko Melis Kant Pawel Jaruga Michael P Stone Miral Dizdaroglu Amanda K McCullough R Stephen Lloyd Source Type: research
Functional analyses of single nucleotide polymorphic variants of the DNA glycosylase NEIL1 present in sub-Saharan African populations
This study provides insight regarding the potential increased risk for early-onset HCC in human populations carrying the NEIL1 I182M variant.PMID:37517321 | DOI:10.1016/j.dnarep.2023.103544 (Source: DNA Repair)
Source: DNA Repair - July 30, 2023 Category: Genetics & Stem Cells Authors: Jamie T Zuckerman Irina G Minko Melis Kant Pawel Jaruga Michael P Stone Miral Dizdaroglu Amanda K McCullough R Stephen Lloyd Source Type: research
Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand
DNA Repair (Amst). 2023 Jul 7;129:103541. doi: 10.1016/j.dnarep.2023.103541. Online ahead of print.ABSTRACTThe generally accepted model assumes that leading strand synthesis is performed by Pol ε, while lagging-strand synthesis is catalyzed by Pol δ. Pol ε has been shown to target the leading strand by interacting with the CMG helicase [Cdc45 Mcm2-7 GINS(Psf1-3, Sld5)]. Proper functioning of the CMG-Pol ɛ, the helicase-polymerase complex is essential for its progression and the fidelity of DNA replication. Dpb2p, the essential non-catalytic subunit of Pol ε plays a key role in maintaining the correct architecture of t...
Source: DNA Repair - July 23, 2023 Category: Genetics & Stem Cells Authors: Michal Dmowski Karolina Makiela-Dzbenska Sushma Sharma Andrei Chabes Iwona J Fijalkowska Source Type: research
Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand
DNA Repair (Amst). 2023 Jul 7;129:103541. doi: 10.1016/j.dnarep.2023.103541. Online ahead of print.ABSTRACTThe generally accepted model assumes that leading strand synthesis is performed by Pol ε, while lagging-strand synthesis is catalyzed by Pol δ. Pol ε has been shown to target the leading strand by interacting with the CMG helicase [Cdc45 Mcm2-7 GINS(Psf1-3, Sld5)]. Proper functioning of the CMG-Pol ɛ, the helicase-polymerase complex is essential for its progression and the fidelity of DNA replication. Dpb2p, the essential non-catalytic subunit of Pol ε plays a key role in maintaining the correct architecture of t...
Source: DNA Repair - July 23, 2023 Category: Genetics & Stem Cells Authors: Michal Dmowski Karolina Makiela-Dzbenska Sushma Sharma Andrei Chabes Iwona J Fijalkowska Source Type: research
Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand
DNA Repair (Amst). 2023 Jul 7;129:103541. doi: 10.1016/j.dnarep.2023.103541. Online ahead of print.ABSTRACTThe generally accepted model assumes that leading strand synthesis is performed by Pol ε, while lagging-strand synthesis is catalyzed by Pol δ. Pol ε has been shown to target the leading strand by interacting with the CMG helicase [Cdc45 Mcm2-7 GINS(Psf1-3, Sld5)]. Proper functioning of the CMG-Pol ɛ, the helicase-polymerase complex is essential for its progression and the fidelity of DNA replication. Dpb2p, the essential non-catalytic subunit of Pol ε plays a key role in maintaining the correct architecture of t...
Source: DNA Repair - July 23, 2023 Category: Genetics & Stem Cells Authors: Michal Dmowski Karolina Makiela-Dzbenska Sushma Sharma Andrei Chabes Iwona J Fijalkowska Source Type: research
Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand
DNA Repair (Amst). 2023 Jul 7;129:103541. doi: 10.1016/j.dnarep.2023.103541. Online ahead of print.ABSTRACTThe generally accepted model assumes that leading strand synthesis is performed by Pol ε, while lagging-strand synthesis is catalyzed by Pol δ. Pol ε has been shown to target the leading strand by interacting with the CMG helicase [Cdc45 Mcm2-7 GINS(Psf1-3, Sld5)]. Proper functioning of the CMG-Pol ɛ, the helicase-polymerase complex is essential for its progression and the fidelity of DNA replication. Dpb2p, the essential non-catalytic subunit of Pol ε plays a key role in maintaining the correct architecture of t...
Source: DNA Repair - July 23, 2023 Category: Genetics & Stem Cells Authors: Michal Dmowski Karolina Makiela-Dzbenska Sushma Sharma Andrei Chabes Iwona J Fijalkowska Source Type: research
