Comparing Mfd- and UvrD-dependent models of transcription coupled DNA repair in live Escherichia coli using single-molecule tracking
DNA Repair (Amst). 2024 Mar 7;137:103665. doi: 10.1016/j.dnarep.2024.103665. Online ahead of print.ABSTRACTDuring transcription-coupled DNA repair (TCR) the detection of DNA damage and initiation of nucleotide excision repair (NER) is performed by translocating RNA polymerases (RNAP), which are arrested upon encountering bulky DNA lesions. Two opposing models of the subsequent steps of TCR in bacteria exist. In the first model, stalled RNAPs are removed from the damage site by recruitment of Mfd which dislodges RNAP by pushing it forwards before recruitment of UvrA and UvrB. In the second model, UvrD helicase backtracks RN...
Source: DNA Repair - March 21, 2024 Category: Genetics & Stem Cells Authors: El żbieta Kaja Donata Vijande Justyna Kowalczyk Micha ł Michalak Jacek Gapi ński Carolin Kobras Philippa Rolfe Mathew Stracy Source Type: research
Comparing Mfd- and UvrD-dependent models of transcription coupled DNA repair in live Escherichia coli using single-molecule tracking
DNA Repair (Amst). 2024 Mar 7;137:103665. doi: 10.1016/j.dnarep.2024.103665. Online ahead of print.ABSTRACTDuring transcription-coupled DNA repair (TCR) the detection of DNA damage and initiation of nucleotide excision repair (NER) is performed by translocating RNA polymerases (RNAP), which are arrested upon encountering bulky DNA lesions. Two opposing models of the subsequent steps of TCR in bacteria exist. In the first model, stalled RNAPs are removed from the damage site by recruitment of Mfd which dislodges RNAP by pushing it forwards before recruitment of UvrA and UvrB. In the second model, UvrD helicase backtracks RN...
Source: DNA Repair - March 21, 2024 Category: Genetics & Stem Cells Authors: El żbieta Kaja Donata Vijande Justyna Kowalczyk Micha ł Michalak Jacek Gapi ński Carolin Kobras Philippa Rolfe Mathew Stracy Source Type: research
Comparing Mfd- and UvrD-dependent models of transcription coupled DNA repair in live Escherichia coli using single-molecule tracking
DNA Repair (Amst). 2024 Mar 7;137:103665. doi: 10.1016/j.dnarep.2024.103665. Online ahead of print.ABSTRACTDuring transcription-coupled DNA repair (TCR) the detection of DNA damage and initiation of nucleotide excision repair (NER) is performed by translocating RNA polymerases (RNAP), which are arrested upon encountering bulky DNA lesions. Two opposing models of the subsequent steps of TCR in bacteria exist. In the first model, stalled RNAPs are removed from the damage site by recruitment of Mfd which dislodges RNAP by pushing it forwards before recruitment of UvrA and UvrB. In the second model, UvrD helicase backtracks RN...
Source: DNA Repair - March 21, 2024 Category: Genetics & Stem Cells Authors: El żbieta Kaja Donata Vijande Justyna Kowalczyk Micha ł Michalak Jacek Gapi ński Carolin Kobras Philippa Rolfe Mathew Stracy Source Type: research
Comparing Mfd- and UvrD-dependent models of transcription coupled DNA repair in live Escherichia coli using single-molecule tracking
DNA Repair (Amst). 2024 Mar 7;137:103665. doi: 10.1016/j.dnarep.2024.103665. Online ahead of print.ABSTRACTDuring transcription-coupled DNA repair (TCR) the detection of DNA damage and initiation of nucleotide excision repair (NER) is performed by translocating RNA polymerases (RNAP), which are arrested upon encountering bulky DNA lesions. Two opposing models of the subsequent steps of TCR in bacteria exist. In the first model, stalled RNAPs are removed from the damage site by recruitment of Mfd which dislodges RNAP by pushing it forwards before recruitment of UvrA and UvrB. In the second model, UvrD helicase backtracks RN...
Source: DNA Repair - March 21, 2024 Category: Genetics & Stem Cells Authors: El żbieta Kaja Donata Vijande Justyna Kowalczyk Micha ł Michalak Jacek Gapi ński Carolin Kobras Philippa Rolfe Mathew Stracy Source Type: research
Comparing Mfd- and UvrD-dependent models of transcription coupled DNA repair in live Escherichia coli using single-molecule tracking
DNA Repair (Amst). 2024 Mar 7;137:103665. doi: 10.1016/j.dnarep.2024.103665. Online ahead of print.ABSTRACTDuring transcription-coupled DNA repair (TCR) the detection of DNA damage and initiation of nucleotide excision repair (NER) is performed by translocating RNA polymerases (RNAP), which are arrested upon encountering bulky DNA lesions. Two opposing models of the subsequent steps of TCR in bacteria exist. In the first model, stalled RNAPs are removed from the damage site by recruitment of Mfd which dislodges RNAP by pushing it forwards before recruitment of UvrA and UvrB. In the second model, UvrD helicase backtracks RN...
Source: DNA Repair - March 21, 2024 Category: Genetics & Stem Cells Authors: El żbieta Kaja Donata Vijande Justyna Kowalczyk Micha ł Michalak Jacek Gapi ński Carolin Kobras Philippa Rolfe Mathew Stracy Source Type: research
SMC-5/6 complex subunit NSE-1 plays a crucial role in meiosis and DNA repair in Caenorhabditis elegans
This study delves into the role of nse-1 in Caenorhabditis elegans, revealing its involvement in meiotic recombination and DNA repair. nse-1 mutants display reduced fertility, increased male incidence, and increased sensitivity to genotoxic chemicals due to defects in meiotic chromosome segregation and DNA repair. These defects manifest as increased accumulation of RAD-51 foci, increased chromosome fragmentation, and susceptibility to MMS, cisplatin, and HU. Furthermore, nse-1 mutation exacerbates germ cell death by upregulating ced-13 and egl-1 genes involved in the CEP-1/p53-mediated apoptotic pathway. NSE-1 is essential...
Source: DNA Repair - March 20, 2024 Category: Genetics & Stem Cells Authors: Arome Solomon Odiba Chiemekam Samuel Ezechukwu Guiyan Liao Ye Hong Wenxia Fang Cheng Jin Anton Gartner Bin Wang Source Type: research
SMC-5/6 complex subunit NSE-1 plays a crucial role in meiosis and DNA repair in Caenorhabditis elegans
This study delves into the role of nse-1 in Caenorhabditis elegans, revealing its involvement in meiotic recombination and DNA repair. nse-1 mutants display reduced fertility, increased male incidence, and increased sensitivity to genotoxic chemicals due to defects in meiotic chromosome segregation and DNA repair. These defects manifest as increased accumulation of RAD-51 foci, increased chromosome fragmentation, and susceptibility to MMS, cisplatin, and HU. Furthermore, nse-1 mutation exacerbates germ cell death by upregulating ced-13 and egl-1 genes involved in the CEP-1/p53-mediated apoptotic pathway. NSE-1 is essential...
Source: DNA Repair - March 20, 2024 Category: Genetics & Stem Cells Authors: Arome Solomon Odiba Chiemekam Samuel Ezechukwu Guiyan Liao Ye Hong Wenxia Fang Cheng Jin Anton Gartner Bin Wang Source Type: research
Mitochondrial transcription factor A (TFAM) has 5'-deoxyribose phosphate lyase activity in vitro
DNA Repair (Amst). 2024 Mar 8;137:103666. doi: 10.1016/j.dnarep.2024.103666. Online ahead of print.ABSTRACTMitochondrial DNA (mtDNA) plays a key role in mitochondrial and cellular functions. mtDNA is maintained by active DNA turnover and base excision repair (BER). In BER, one of the toxic repair intermediates is 5'-deoxyribose phosphate (5'dRp). Human mitochondrial DNA polymerase γ has weak dRp lyase activities, and another known dRp lyase in the nucleus, human DNA polymerase β, can also localize to mitochondria in certain cell and tissue types. Nonetheless, whether additional proteins have the ability to remove 5'dRp i...
Source: DNA Repair - March 16, 2024 Category: Genetics & Stem Cells Authors: Wenxin Zhao Adil S Hussen Bret D Freudenthal Zucai Suo Linlin Zhao Source Type: research
Mitochondrial transcription factor A (TFAM) has 5'-deoxyribose phosphate lyase activity in vitro
DNA Repair (Amst). 2024 Mar 8;137:103666. doi: 10.1016/j.dnarep.2024.103666. Online ahead of print.ABSTRACTMitochondrial DNA (mtDNA) plays a key role in mitochondrial and cellular functions. mtDNA is maintained by active DNA turnover and base excision repair (BER). In BER, one of the toxic repair intermediates is 5'-deoxyribose phosphate (5'dRp). Human mitochondrial DNA polymerase γ has weak dRp lyase activities, and another known dRp lyase in the nucleus, human DNA polymerase β, can also localize to mitochondria in certain cell and tissue types. Nonetheless, whether additional proteins have the ability to remove 5'dRp i...
Source: DNA Repair - March 16, 2024 Category: Genetics & Stem Cells Authors: Wenxin Zhao Adil S Hussen Bret D Freudenthal Zucai Suo Linlin Zhao Source Type: research
Mitochondrial transcription factor A (TFAM) has 5'-deoxyribose phosphate lyase activity in vitro
DNA Repair (Amst). 2024 Mar 8;137:103666. doi: 10.1016/j.dnarep.2024.103666. Online ahead of print.ABSTRACTMitochondrial DNA (mtDNA) plays a key role in mitochondrial and cellular functions. mtDNA is maintained by active DNA turnover and base excision repair (BER). In BER, one of the toxic repair intermediates is 5'-deoxyribose phosphate (5'dRp). Human mitochondrial DNA polymerase γ has weak dRp lyase activities, and another known dRp lyase in the nucleus, human DNA polymerase β, can also localize to mitochondria in certain cell and tissue types. Nonetheless, whether additional proteins have the ability to remove 5'dRp i...
Source: DNA Repair - March 16, 2024 Category: Genetics & Stem Cells Authors: Wenxin Zhao Adil S Hussen Bret D Freudenthal Zucai Suo Linlin Zhao Source Type: research
Deletions initiated by the vaccinia virus TopIB protein in yeast
DNA Repair (Amst). 2024 Mar 6;137:103664. doi: 10.1016/j.dnarep.2024.103664. Online ahead of print.ABSTRACTThe type IB topoisomerase of budding yeast (yTop1) generates small deletions in tandem repeats through a sequential cleavage mechanism and larger deletions with random endpoints through the nonhomologous end-joining (NHEJ) pathway. Vaccinia virus Top1 (vTop1) is a minimized version of the eukaryal TopIB enzymes and uniquely has a strong consensus cleavage sequence: the pentanucleotide (T/C)CCTTp↓. To define the relationship between the position of TopIB cleavage and mutagenic outcomes, we expressed vTop1 in yeast to...
Source: DNA Repair - March 14, 2024 Category: Genetics & Stem Cells Authors: Jang Eun Cho Samantha Shaltz Lyudmila Yakovleva Stewart Shuman Sue Jinks-Robertson Source Type: research
Deletions initiated by the vaccinia virus TopIB protein in yeast
DNA Repair (Amst). 2024 Mar 6;137:103664. doi: 10.1016/j.dnarep.2024.103664. Online ahead of print.ABSTRACTThe type IB topoisomerase of budding yeast (yTop1) generates small deletions in tandem repeats through a sequential cleavage mechanism and larger deletions with random endpoints through the nonhomologous end-joining (NHEJ) pathway. Vaccinia virus Top1 (vTop1) is a minimized version of the eukaryal TopIB enzymes and uniquely has a strong consensus cleavage sequence: the pentanucleotide (T/C)CCTTp↓. To define the relationship between the position of TopIB cleavage and mutagenic outcomes, we expressed vTop1 in yeast to...
Source: DNA Repair - March 14, 2024 Category: Genetics & Stem Cells Authors: Jang Eun Cho Samantha Shaltz Lyudmila Yakovleva Stewart Shuman Sue Jinks-Robertson Source Type: research
Deletions initiated by the vaccinia virus TopIB protein in yeast
DNA Repair (Amst). 2024 Mar 6;137:103664. doi: 10.1016/j.dnarep.2024.103664. Online ahead of print.ABSTRACTThe type IB topoisomerase of budding yeast (yTop1) generates small deletions in tandem repeats through a sequential cleavage mechanism and larger deletions with random endpoints through the nonhomologous end-joining (NHEJ) pathway. Vaccinia virus Top1 (vTop1) is a minimized version of the eukaryal TopIB enzymes and uniquely has a strong consensus cleavage sequence: the pentanucleotide (T/C)CCTTp↓. To define the relationship between the position of TopIB cleavage and mutagenic outcomes, we expressed vTop1 in yeast to...
Source: DNA Repair - March 14, 2024 Category: Genetics & Stem Cells Authors: Jang Eun Cho Samantha Shaltz Lyudmila Yakovleva Stewart Shuman Sue Jinks-Robertson Source Type: research
Dominant roles of BRCA1 in cellular tolerance to a chain-terminating nucleoside analog, alovudine
DNA Repair (Amst). 2024 Mar 5;137:103668. doi: 10.1016/j.dnarep.2024.103668. Online ahead of print.ABSTRACTAlovudine is a chain-terminating nucleoside analog (CTNA) that is frequently used as an antiviral and anticancer agent. Generally, CTNAs inhibit DNA replication after their incorporation into nascent DNA during DNA synthesis by suppressing subsequent polymerization, which restricts the proliferation of viruses and cancer cells. Alovudine is a thymidine analog used as an antiviral drug. However, the mechanisms underlying the removal of alovudine and DNA damage tolerance pathways involved in cellular resistance to alovu...
Source: DNA Repair - March 9, 2024 Category: Genetics & Stem Cells Authors: Md Bayejid Hosen Ryotaro Kawasumi Kouji Hirota Source Type: research
Dominant roles of BRCA1 in cellular tolerance to a chain-terminating nucleoside analog, alovudine
DNA Repair (Amst). 2024 Mar 5;137:103668. doi: 10.1016/j.dnarep.2024.103668. Online ahead of print.ABSTRACTAlovudine is a chain-terminating nucleoside analog (CTNA) that is frequently used as an antiviral and anticancer agent. Generally, CTNAs inhibit DNA replication after their incorporation into nascent DNA during DNA synthesis by suppressing subsequent polymerization, which restricts the proliferation of viruses and cancer cells. Alovudine is a thymidine analog used as an antiviral drug. However, the mechanisms underlying the removal of alovudine and DNA damage tolerance pathways involved in cellular resistance to alovu...
Source: DNA Repair - March 9, 2024 Category: Genetics & Stem Cells Authors: Md Bayejid Hosen Ryotaro Kawasumi Kouji Hirota Source Type: research
