Catalytic activity of OGG1 is impaired by Zinc deficiency
DNA Repair (Amst). 2024 Feb;134:103628. doi: 10.1016/j.dnarep.2024.103628. Epub 2024 Jan 10.ABSTRACTOxidative stress-induced DNA base modifications, if unrepaired, can increase mutagenesis and genomic instability, ultimately leading to cell death. Cells predominantly use the base excision repair (BER) pathway to repair oxidatively-induced non-helix distorting lesions. BER is initiated by DNA glycosylases, such as 8-oxoguanine DNA glycosylase (OGG1), which repairs oxidatively modified guanine bases, including 7,8-dihydro-8-oxoguanine (8-oxoG) and ring-opened formamidopyrimidine lesions, 2,6-diamino-4-hydroxy-5-formamidopyri...
Source: DNA Repair - January 16, 2024 Category: Genetics & Stem Cells Authors: Priyanka Sharma Carmen P Wong Emily Ho Harini Sampath Source Type: research
Ku80 is indispensable for repairing DNA double-strand breaks at highly methylated sites in human HCT116 cells
In this study, we used CRISPR to generate Ku80-/- human cells and arrested the cells in G1 phase to accumulate DSBs before conducting END-seq and Nanopore analysis. Our analysis revealed that DNA with high methylation level accumulates DSB hotspots in Ku80-/- human cells. Furthermore, we identified chromosome structural variants (SVs) using Nanopore sequencing and observed a higher number of SVs in Ku80-/- human cells. Based on our findings, we suggest that the high efficiency of Ku80 knockout in human HCT116 cells makes it a promising model for characterizing SVs in the context of 3D chromatin structure and studying the a...
Source: DNA Repair - January 14, 2024 Category: Genetics & Stem Cells Authors: Mengtan Xing Yanhong Xiong Yong Zhang Source Type: research
Corrigendum to "Over-expression of miR-100 is responsible for the low-expression of ATM in the human glioma cell line: M059J" [DNA Repair 9 (2010) 1170-1175]
DNA Repair (Amst). 2024 Feb;134:103631. doi: 10.1016/j.dnarep.2024.103631. Epub 2024 Jan 13.NO ABSTRACTPMID:38217905 | DOI:10.1016/j.dnarep.2024.103631 (Source: DNA Repair)
Source: DNA Repair - January 13, 2024 Category: Genetics & Stem Cells Authors: Wooi Loon Ng Dan Yan Xiangming Zhang Yin-Yuan Mo Ya Wang Source Type: research
DONSON: Slding in 2 the limelight
DNA Repair (Amst). 2024 Feb;134:103616. doi: 10.1016/j.dnarep.2023.103616. Epub 2023 Dec 23.ABSTRACTFor over a decade, it has been known that yeast Sld2, Dpb11, GINS and Polε form the pre-loading complex (pre-LC), which is recruited to a CDC45-bound MCM2-7 complex by the Sld3/Sld7 heterodimer in a phospho-dependent manner. Whilst functional orthologs of Dbp11 (TOPBP1), Sld3 (TICRR) and Sld7 (MTBP) have been identified in metazoans, controversy has surrounded the identity of the Sld2 ortholog. It was originally proposed that the RECQ helicase, RECQL4, which is mutated in Rothmund-Thomson syndrome, represented the closest v...
Source: DNA Repair - December 30, 2023 Category: Genetics & Stem Cells Authors: Grant S Stewart Source Type: research
DONSON: Slding in 2 the limelight
DNA Repair (Amst). 2023 Dec 23;134:103616. doi: 10.1016/j.dnarep.2023.103616. Online ahead of print.ABSTRACTFor over a decade, it has been known that yeast Sld2, Dpb11, GINS and Polε form the pre-loading complex (pre-LC), which is recruited to a CDC45-bound MCM2-7 complex by the Sld3/Sld7 heterodimer in a phospho-dependent manner. Whilst functional orthologs of Dbp11 (TOPBP1), Sld3 (TICRR) and Sld7 (MTBP) have been identified in metazoans, controversy has surrounded the identity of the Sld2 ortholog. It was originally proposed that the RECQ helicase, RECQL4, which is mutated in Rothmund-Thomson syndrome, represented the c...
Source: DNA Repair - December 30, 2023 Category: Genetics & Stem Cells Authors: Grant S Stewart Source Type: research
DONSON: Slding in 2 the limelight
DNA Repair (Amst). 2023 Dec 23;134:103616. doi: 10.1016/j.dnarep.2023.103616. Online ahead of print.ABSTRACTFor over a decade, it has been known that yeast Sld2, Dpb11, GINS and Polε form the pre-loading complex (pre-LC), which is recruited to a CDC45-bound MCM2-7 complex by the Sld3/Sld7 heterodimer in a phospho-dependent manner. Whilst functional orthologs of Dbp11 (TOPBP1), Sld3 (TICRR) and Sld7 (MTBP) have been identified in metazoans, controversy has surrounded the identity of the Sld2 ortholog. It was originally proposed that the RECQ helicase, RECQL4, which is mutated in Rothmund-Thomson syndrome, represented the c...
Source: DNA Repair - December 30, 2023 Category: Genetics & Stem Cells Authors: Grant S Stewart Source Type: research
DONSON: Slding in 2 the limelight
DNA Repair (Amst). 2023 Dec 23;134:103616. doi: 10.1016/j.dnarep.2023.103616. Online ahead of print.ABSTRACTFor over a decade, it has been known that yeast Sld2, Dpb11, GINS and Polε form the pre-loading complex (pre-LC), which is recruited to a CDC45-bound MCM2-7 complex by the Sld3/Sld7 heterodimer in a phospho-dependent manner. Whilst functional orthologs of Dbp11 (TOPBP1), Sld3 (TICRR) and Sld7 (MTBP) have been identified in metazoans, controversy has surrounded the identity of the Sld2 ortholog. It was originally proposed that the RECQ helicase, RECQL4, which is mutated in Rothmund-Thomson syndrome, represented the c...
Source: DNA Repair - December 30, 2023 Category: Genetics & Stem Cells Authors: Grant S Stewart Source Type: research
DONSON: Slding in 2 the limelight
DNA Repair (Amst). 2023 Dec 23;134:103616. doi: 10.1016/j.dnarep.2023.103616. Online ahead of print.ABSTRACTFor over a decade, it has been known that yeast Sld2, Dpb11, GINS and Polε form the pre-loading complex (pre-LC), which is recruited to a CDC45-bound MCM2-7 complex by the Sld3/Sld7 heterodimer in a phospho-dependent manner. Whilst functional orthologs of Dbp11 (TOPBP1), Sld3 (TICRR) and Sld7 (MTBP) have been identified in metazoans, controversy has surrounded the identity of the Sld2 ortholog. It was originally proposed that the RECQ helicase, RECQL4, which is mutated in Rothmund-Thomson syndrome, represented the c...
Source: DNA Repair - December 30, 2023 Category: Genetics & Stem Cells Authors: Grant S Stewart Source Type: research
DONSON: Slding in 2 the limelight
DNA Repair (Amst). 2023 Dec 23;134:103616. doi: 10.1016/j.dnarep.2023.103616. Online ahead of print.ABSTRACTFor over a decade, it has been known that yeast Sld2, Dpb11, GINS and Polε form the pre-loading complex (pre-LC), which is recruited to a CDC45-bound MCM2-7 complex by the Sld3/Sld7 heterodimer in a phospho-dependent manner. Whilst functional orthologs of Dbp11 (TOPBP1), Sld3 (TICRR) and Sld7 (MTBP) have been identified in metazoans, controversy has surrounded the identity of the Sld2 ortholog. It was originally proposed that the RECQ helicase, RECQL4, which is mutated in Rothmund-Thomson syndrome, represented the c...
Source: DNA Repair - December 30, 2023 Category: Genetics & Stem Cells Authors: Grant S Stewart Source Type: research
DONSON: Slding in 2 the limelight
DNA Repair (Amst). 2023 Dec 23;134:103616. doi: 10.1016/j.dnarep.2023.103616. Online ahead of print.ABSTRACTFor over a decade, it has been known that yeast Sld2, Dpb11, GINS and Polε form the pre-loading complex (pre-LC), which is recruited to a CDC45-bound MCM2-7 complex by the Sld3/Sld7 heterodimer in a phospho-dependent manner. Whilst functional orthologs of Dbp11 (TOPBP1), Sld3 (TICRR) and Sld7 (MTBP) have been identified in metazoans, controversy has surrounded the identity of the Sld2 ortholog. It was originally proposed that the RECQ helicase, RECQL4, which is mutated in Rothmund-Thomson syndrome, represented the c...
Source: DNA Repair - December 30, 2023 Category: Genetics & Stem Cells Authors: Grant S Stewart Source Type: research
Biochemical analysis of H < sub > 2 < /sub > O < sub > 2 < /sub > -induced mutation spectra revealed that multiple damages were involved in the mutational process
DNA Repair (Amst). 2023 Dec 22;134:103617. doi: 10.1016/j.dnarep.2023.103617. Online ahead of print.ABSTRACTReactive oxygen species (ROS) are a major threat to genomic integrity and believed to be one of the etiologies of cancers. Here we developed a cell-free system to analyze ROS-induced mutagenesis, in which DNA was exposed to H2O2 and then subjected to translesion DNA synthesis by various DNA polymerases. Then, frequencies of mutations on the DNA products were determined by using next-generation sequencing technology. The majority of observed mutations were either C>A or G>A, caused by dAMP insertion at G and C r...
Source: DNA Repair - December 28, 2023 Category: Genetics & Stem Cells Authors: Tomohiko Sugiyama Mahima R Sanyal Source Type: research
Biochemical analysis of H < sub > 2 < /sub > O < sub > 2 < /sub > -induced mutation spectra revealed that multiple damages were involved in the mutational process
DNA Repair (Amst). 2023 Dec 22;134:103617. doi: 10.1016/j.dnarep.2023.103617. Online ahead of print.ABSTRACTReactive oxygen species (ROS) are a major threat to genomic integrity and believed to be one of the etiologies of cancers. Here we developed a cell-free system to analyze ROS-induced mutagenesis, in which DNA was exposed to H2O2 and then subjected to translesion DNA synthesis by various DNA polymerases. Then, frequencies of mutations on the DNA products were determined by using next-generation sequencing technology. The majority of observed mutations were either C>A or G>A, caused by dAMP insertion at G and C r...
Source: DNA Repair - December 28, 2023 Category: Genetics & Stem Cells Authors: Tomohiko Sugiyama Mahima R Sanyal Source Type: research
Biochemical analysis of H < sub > 2 < /sub > O < sub > 2 < /sub > -induced mutation spectra revealed that multiple damages were involved in the mutational process
DNA Repair (Amst). 2023 Dec 22;134:103617. doi: 10.1016/j.dnarep.2023.103617. Online ahead of print.ABSTRACTReactive oxygen species (ROS) are a major threat to genomic integrity and believed to be one of the etiologies of cancers. Here we developed a cell-free system to analyze ROS-induced mutagenesis, in which DNA was exposed to H2O2 and then subjected to translesion DNA synthesis by various DNA polymerases. Then, frequencies of mutations on the DNA products were determined by using next-generation sequencing technology. The majority of observed mutations were either C>A or G>A, caused by dAMP insertion at G and C r...
Source: DNA Repair - December 28, 2023 Category: Genetics & Stem Cells Authors: Tomohiko Sugiyama Mahima R Sanyal Source Type: research
Biochemical analysis of H < sub > 2 < /sub > O < sub > 2 < /sub > -induced mutation spectra revealed that multiple damages were involved in the mutational process
DNA Repair (Amst). 2023 Dec 22;134:103617. doi: 10.1016/j.dnarep.2023.103617. Online ahead of print.ABSTRACTReactive oxygen species (ROS) are a major threat to genomic integrity and believed to be one of the etiologies of cancers. Here we developed a cell-free system to analyze ROS-induced mutagenesis, in which DNA was exposed to H2O2 and then subjected to translesion DNA synthesis by various DNA polymerases. Then, frequencies of mutations on the DNA products were determined by using next-generation sequencing technology. The majority of observed mutations were either C>A or G>A, caused by dAMP insertion at G and C r...
Source: DNA Repair - December 28, 2023 Category: Genetics & Stem Cells Authors: Tomohiko Sugiyama Mahima R Sanyal Source Type: research
Contents of previous 3 special issues in this series of perspectives
DNA Repair (Amst). 2023 Dec 20;134:103615. doi: 10.1016/j.dnarep.2023.103615. Online ahead of print.NO ABSTRACTPMID:38142594 | DOI:10.1016/j.dnarep.2023.103615 (Source: DNA Repair)
Source: DNA Repair - December 24, 2023 Category: Genetics & Stem Cells Authors: Penny Jeggo Source Type: research
