Synergy of a virulent phage ( φAB182) with antibiotics leading to successful elimination of biofilms formed by MDR Acinetobacter baumannii
In this report, we have described the synergy assessment of a virulent Acinetobacter baumannii phage φAB182 with a wide range of antibiotics. Myophage φAB182 was isolated from sewage against MDR A. baumannii and exhibited maximum stability at 25 °C and pH 7. It also had a short latent period of 9 min with a large burst size of 287. The phylogenetic analysis of its major capsid protein gene indi cated an 84.15% similarity to the lytic A. baumannii phage Acj9. In the presence of antibiotics, phage φAB182 showed the highest synergy (p < 0.0001) with colistin, followed by polymixin B, ceftazidime and cefotaxime and...
Source: Canadian Journal of Microbiology - September 29, 2022 Category: Microbiology Authors: Medhavi Vashisth Shikha Yashveer Anu Bala Jaglan Nitin Virmani Bidhan Chand Bera Rajesh Kumar Vaid Taruna Anand Source Type: research
Correlation between SAP2 and CAP1 in clinical strains of Candida albicans at planktonic and biofilm states
This study aimed to explore the influences of SAP2 and CAP1 on itraconazole (ITR) resistance of Candida albicans at different states. A total of 10 ITR-resistant strains and 10 ITR-sensitive strains were used for SAP2 sequencing and CAP1 sequencing. SAP2 sequencing showed no missense mutation, and three synonymous mutations. CAP1 gene sequencing identified two missense mutations M140I (8) and K191Q (4), and 14 synonymous mutations G201A (1), A246C (5), C282T (6), G288A (6), C321T (7), A399C (16), C432T (16), C465T (11), G552A (16), G669T (1), G672A (1), G681T (2), T783C (1), and T819A (2). The biofilm formation capacity of...
Source: Canadian Journal of Microbiology - September 26, 2022 Category: Microbiology Authors: Wenli Feng Jing Yang Yan Ma Yinglu Liu Rong Yin Zusha Qiao Ying Ji Yong'an Zhou Source Type: research
Comparative lipidomics of Pichia pastoris using constitutive promoter reveals lipid diversity and variability at different growth phases
Canadian Journal of Microbiology, Ahead of Print. Pichia pastoris is an expression platform widely used for foreign protein expression, while it is unknown how the global lipid profiles changed during the cultivation process, which is crucial for fermentation optimization and chassis design. Therefore, this study aimed to reveal the diverse lipid profiles of P. pastoris controlled by constitutive promoter of glyceraldehyde-3-phosphate dehydrogenase gene and to unravel their change in the lag, logarithmic, stationary, and death phases, using ultra-performance liquid chromatography/nano-electrospray ionization-tandem mass sp...
Source: Canadian Journal of Microbiology - September 21, 2022 Category: Microbiology Authors: Keyang Yu Zhaojun Liu Shuyan Wu Shuanghua Luo Xing Weng Zhou Wang Maomao Zeng Xiaoyuan Wang Xiaoqing Hu Source Type: research
The effect of Escherichia coli ŽP strain with a conjugation-based colicin E7 delivery on growth performance, hematological, biochemical, and histological parameters, gut microbiota, and nonspecific immunity of broilers
Canadian Journal of Microbiology, Ahead of Print. The Escherichia coli ŽP strain (ŽP) was constructed based on the known probiotic E. coli strain Nissle 1917. It was genetically modified to carry the colicin E7 synthesis gene encoding DNase on a conjugative plasmid and the colicin E7 immunity gene in the chromosome. The aim of this study was to evaluate the effects of the daily ŽP per oral administration (5 × 108 or 5 × 1010 CFU per bird) on the growth performance, hematological, biochemical, histological parameters, gut microbiota, and nonspecific immunity of the 4–24 days old broilers. The ŽP administra...
Source: Canadian Journal of Microbiology - September 19, 2022 Category: Microbiology Authors: E.G. Orlova I.L. Maslennikova J.S. Pospelova M. Star čič Erjavec N.P. Loginova Y.N. Troinich M.V. Kuznetsova Source Type: research
Analysis of microbial community diversity and physicochemical factors in pit mud of different ages based on high-throughput sequencing
In this study, Illumina MiSeq/NovaSeq high-throughput sequencing technology was used to sequence the terminal DNA fragments of microbial communities in Wuliangye pit mud. The results showed that there were 5 dominant bacterial phyla and 13 dominant bacterial genera in the pit mud, which belonged to 4 phyla, mainly Firmicutes. There were 3 dominant fungal phyla and 5 dominant fungal genera in cellar mud, which belonged to 2 phyla and concentrated in Ascomycota. According to the statistical data, the low pH value cellar pool is more conducive to the enrichment of acid-resistant or acid-biased bacteria, which is the key to fl...
Source: Canadian Journal of Microbiology - September 14, 2022 Category: Microbiology Authors: Yan Gong Na Ma Hui Tang Source Type: research
Assessing dual use research of concern (DURC) —lessons learned from the United States government institutional DURC policy
Canadian Journal of Microbiology, Ahead of Print. Life science research was analyzed for potential misuse in the 2004 report “Biotechnology Research in an Age of Bioterrorism”. However, it was not until 2015 that the United States Government (USG) Institutional Dual Use Research of Concern (DURC) policy went into effect. Institutions receiving USG funding for life science research are required to scan their research p ortfolios for research involving one of 15 agents and subsequent 7 experimental effects described in the policy. In practice, this policy was implemented in a variety of ways with varying outcomes and les...
Source: Canadian Journal of Microbiology - August 30, 2022 Category: Microbiology Authors: Rebecca Moritz Source Type: research
Whole genome sequencing of mesorhizobia isolated from northern Canada
Canadian Journal of Microbiology, Ahead of Print. Rhizobia are soil-dwelling bacteria that can form N2-fixing symbioses with legume plant species (Fabaceae). These bacteria are globally distributed; however, few studies have examined the genomics of rhizobia that live in cold environments. Here, we isolated and characterized three rhizobial strains from legume nodules collected at a pair of distant low Arctic tundra and boreal forest sites in northern Canada. Phylogenetic and average nucleotide identity measurements suggested that the three strains are members of the genus Mesorhizobium, and that each strain represents a n...
Source: Canadian Journal of Microbiology - August 18, 2022 Category: Microbiology Authors: Yi Fan Duan Paul Grogan Virginia K Walker George C diCenzo Source Type: research
Temporal variation in skin microbiota of cohabitating amphibians
Canadian Journal of Microbiology, Ahead of Print. Temporal changes and transmission patterns in host-associated microbial communities have important implications for host health. The diversity of amphibian skin microbial communities is associated with disease outcome in amphibians exposed to the fungal pathogen Batrachochytrium dendrobatidis (Bd). To successfully develop conservation strategies against Bd, we need a comprehensive understanding of how skin microbes are maintained and transmitted over time within populations. We used 16S rRNA sequence analysis to compare Epipedobates anthonyi frogs housed with one conspecifi...
Source: Canadian Journal of Microbiology - August 18, 2022 Category: Microbiology Authors: Ariel Kruger Spencer Roth Source Type: research
Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity
This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin. Litter (27.5 T/ha) was applied to 24 agricultural plots in the Fraser Valley of British Columbia. Nonfertilized plots were used as a negative control. Soil samples collected from the studied plots were used to quantify Escherichia coli by plate counts, and Clostridium perfringens by qPCR. The 16S rRNA gene sequencing was performed for microbiota analysis. Followi...
Source: Canadian Journal of Microbiology - August 9, 2022 Category: Microbiology Authors: Bilal Muhammad Moussa S. Diarra Md Rashedul Islam Dion Lepp Rose E. Mastin-Wood Edward Topp Shabtai Bittman Xin Zhao Source Type: research
Diversity of fungal assemblages in rhizosphere and endosphere of blueberry (Vaccinium spp.) under field conditions revealed by culturing and culture-independent molecular methods
Canadian Journal of Microbiology, Ahead of Print. Mycorrhizae are important to plants in improving nutrient absorption and stress resistance. To study mycorrhizal fungal diversity in blueberry, we combined culture method and culture-independent molecular method to analyze the root endosphere and rhizosphere fungi in three different cultivars. We obtained 212 isolates with a culture method and classified them into 40 types according to their morphological characteristics. Then, we amplified the internal transcribed spacer sequence and found rich species diversity. With high-throughput sequencing, 561 operational taxonomic u...
Source: Canadian Journal of Microbiology - August 4, 2022 Category: Microbiology Authors: Mei Dong Bowei Wang Youwen Tian Li Chen Yadong Li Haiyue Sun Source Type: research
Spatial variability of bacterial community compositions in the Mariana Trench
Canadian Journal of Microbiology, Ahead of Print. Hadal microorganisms play an important role in the biogeochemical processes in marine ecosystems and act as a valuable resource for industrial applications. This paper presents the bacterial community analysis of samples taken from the Challenger Deep within the Mariana Trench, which is the deepest site in the ocean. High-throughput 16S rRNA gene amplicon sequencing was used to reveal that the vertically sampled bacterial populations at eight stations varied at the surface to 10 km depth. The surface water samples harbored a distinct bacterial assemblage, while the mesopela...
Source: Canadian Journal of Microbiology - August 4, 2022 Category: Microbiology Authors: Wei Wang Jingjing Sun Jianhua Hao Source Type: research
A mutation in the putative CRP binding site of the dctA promoter of Salmonella enterica serovar Typhimurium enables growth with low orotate concentrations
Canadian Journal of Microbiology, Ahead of Print. Salmonella enterica and Escherichia coli use the inner membrane transporter DctA to import the pyrimidine biosynthetic pathway intermediate orotate from the environment. To study the regulation of dctA expression, we used an S. enterica serovar Typhimurium pyrimidine auxotroph to select a mutant that could grow in an otherwise nonpermissive culture medium containing glucose and a low concentration of orotate. Whole genome sequencing revealed a point mutation upstream of dctA in the putative cyclic AMP receptor protein (CRP) binding site. The C →T transition converted the ...
Source: Canadian Journal of Microbiology - August 3, 2022 Category: Microbiology Authors: Mohammad R. Amin Lisa Korchinski Joshua K. Yoneda Rishi Thakkar Carla L.A. Sanson Stephen F. Fitzgerald Rod A. Kelln Andrew D.S. Cameron Source Type: research
Degradation of isoproturon in vitro by a mix of bacterial strains isolated from arable soil
In this study, four IPU-degrading bacterial strains, i.e., Sphingomonas sp. ISP1, Arthrobacter sp. ISP2, Acinetobacter baumannii 4IA, and Pseudomonas sp. ISP3, were isolated from agricultural soil. The mixed culture of four isolates completely degraded the herbicide at 100 mg/L within 10 days. During IPU degradation, several transient accumulations of the metabolites, including 3-(4-isopropylphenyl)-1-methylurea, 3-(4-isopropylphenyl)-urea, 4-isopropylaniline, and 4-toluidine, were also identified. Moreover, the inoculation of the isolated mixed culture into the soi l from a mountain with no previous herbicide applicatio...
Source: Canadian Journal of Microbiology - July 27, 2022 Category: Microbiology Authors: Ha Danh Duc Source Type: research
Diet and ontogeny drastically alter the larval microbiome of the invertebrate model Galleria mellonella
Canadian Journal of Microbiology, Ahead of Print. Larvae of the greater wax moth (Galleria mellonella) are an emerging animal model to study the innate immune response and biodegradation of plastic polymers. Both of these complex biological processes are likely impacted by the plasticity of host –microbe interactions, which remains understudied in lepidopterans. Consequently, we carried out 16S rRNA sequencing to explore the effect diet (natural, artificial) has on the bacterial assemblages of G. mellonella in different tissues (gut, fat bodies, silk glands) throughout development (eggs, six instar stages, adults). The m...
Source: Canadian Journal of Microbiology - July 21, 2022 Category: Microbiology Authors: Patrick Gohl Christophe M.R. LeMoine Bryan J. Cassone Source Type: research
Decline of humoral immune responses after natural SARS-CoV-2 infection can be efficiently reversed by vaccination
Canadian Journal of Microbiology, Ahead of Print. Our aim was to analyze severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific antibody level kinetics after coronavirus disease 2019 (COVID-19) infection and determine the efficiency of vaccination on SARS-CoV-2-specific antibody levels. The study included 50 SARS-CoV-2 infected and 70 uninfected cases. Levels of SARS-CoV-2-specific IgG nucleocapsid protein (IgG-NP), IgG spike protein (IgG-SP), IgM nucleocapsid protein (IgM-NP), and IgA spike protein (IgA-SP) antibodies were evaluated by an enzyme-linked immunosorbent assay in sera obtained at baseline, 1st, ...
Source: Canadian Journal of Microbiology - July 19, 2022 Category: Microbiology Authors: Fatma Betul Oktelik Vuslat Yilmaz Metin Yusuf Gelmez Nilgun Akdeniz Cevriye Pamukcu Tolga Sutlu Murat Kose Erdem Tuzun Gunnur Deniz Source Type: research
