Distribution of integrons and phylogenetic groups among Escherichia coli causing community-acquired urinary tract infection in Upper Egypt
In conclusion, the types and combinations of the gene cassettes in our study may reflect the specific selective pressures to which the isolates were subjected within the study region, therefore, providing valuable data for future intervention strategies that are precisely tailored to prevent the dissemination of the uropathogenic E. coli strains circulating within Upper Egypt. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - October 29, 2020 Category: Microbiology Authors: Eman M. Farahat Noha A. Hassuna Adel M. Hammad Medhat Abdel Fattah Ahmed S. Khairalla Source Type: research
Effects of simulated warming on bacterial diversity and abundance in tropical soils from East Malaysia using open top chambers
This study aims to determine soil bacterial diversity in the tropics and their response towards in situ warming using an open-top chamber (OTC). OTCs were set up in areas exposed to sunlight throughout the year in the tropical region in Malaysia. Soil samples were collected every 3 months to monitor changes in bacterial diversity using V3 –V4 16S rDNA amplicon sequencing inside the OTCs (treatment plots) and outside the OTCs (control plots). After 12 months of simulated warming, an average increase of 0.81 to 1.15 °C was recorded in treatment plots. Significant changes in the relative abundance of bacterial phyla such a...
Source: Canadian Journal of Microbiology - October 21, 2020 Category: Microbiology Authors: Chuen Yang Chua Clemente Michael Vui Ling Wong Source Type: research
Understanding connections and roles of gut microbiome in cardiovascular diseases
Canadian Journal of Microbiology, Ahead of Print. The gut microbiome encompasses trillions of residing microbes, mainly bacteria, which play a crucial role in maintaining the physiological and metabolic health of the host. The gut microbiome has been associated with several diseases, including cardiovascular disease (CVD). A growing body of evidence suggests that an altered gut environment and gut-microbiome-derived metabolites are associated with CVD events. The gut microbiome communicates with host physiology through different mechanisms, including trimethylamine N-oxide generation, primary and secondary bile acid metabo...
Source: Canadian Journal of Microbiology - October 20, 2020 Category: Microbiology Authors: Ethendhar Rajendiran Balamurugan Ramadass Vanu Ramprasath Source Type: research
Evolving the core microbial community in pit mud based on bioturbation of fortified Daqu
In this study, the addition of fortified Daqu to artificial PM (APM) was intended to disturb the microbial community and further affect metabolites. To evaluate the effect of fortified Daqu on culturing APM, the microbial communities of APM with or without the addition of fortified Daqu were investigated by fluorescence in situ hybridization and Illumina MiSeq. The results indicated that microbes (Clostridium sp., Clostridium kluyveri, hydrogenotrophic methanogens, and acetotrophic methanogens) related to the production of key aroma compounds increased notably when fortified Daqu was added. In particular, the hydrogenotrop...
Source: Canadian Journal of Microbiology - October 16, 2020 Category: Microbiology Authors: Suqi Chen Jun Huang Hui Qin Guiqiang He Rongqing Zhou Yan Yang Chuanfeng Qiu Suyi Zhang Source Type: research
CEASE approach for combating COVID-19, antimicrobial resistance, and future microbial threats
Canadian Journal of Microbiology, Ahead of Print. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - October 14, 2020 Category: Microbiology Authors: Teddie O. Rahube Source Type: research
qPCR assay targeting Bradyrhizobium japonicum shows that row spacing and soybean density affects Bradyrhizobium population
Canadian Journal of Microbiology, Ahead of Print. The ability for a soybean plant to be efficiently nodulated when grown as a crop is dependent on the number of effective Bradyrhizobium japonicum that can be found in close proximity to the developing seedling shortly after planting. In Manitoba, the growing of soybean as a crop has increased from less than 500 000 acres in 2008 to over 2.3 million acres in 2017. Since the large increase in soybean production is relatively recent, populations of B. japonicum have not yet developed. In response to this, we developed a primer pair that can identify B. japonicum, and be u...
Source: Canadian Journal of Microbiology - October 13, 2020 Category: Microbiology Authors: Harry Yudistira Barney A. Geddes Charles M. Geddes Robert H. Gulden Ivan J. Oresnik Source Type: research
Endophytic fungus diversity in soybean plants submitted to conditions of elevated atmospheric CO2 and temperature
Canadian Journal of Microbiology, Ahead of Print. Global climatic changes can have drastic impacts on plant species, including severe consequences for the agricultural species productivity. Many of these species present important mutualisms with endophytic fungi that positively influence their performance. The present study evaluated whether the increases in CO2 and temperature predicted for the year 2100 may cause changes in foliar carbon (C) and nitrogen (N) concentrations in soybean (Glycine max) and, consequently, the interactions with its endophytic fungi. The effects of elevated CO2 and temperature were evaluated in ...
Source: Canadian Journal of Microbiology - October 8, 2020 Category: Microbiology Authors: Huberman Valadares Gon çalves Yumi Oki Leandra Bordignon Mariana Costa Ferreira Jos é Eustáquio dos Santos Lucas Barbosa Souza Tameir ão Fabr ício Rodrigues Santos Evanguedes Kalapothakis Geraldo Wilson Fernandes Source Type: research
Proteomics of host –bacterial interactions: new insights from dual perspectives
Canadian Journal of Microbiology, Ahead of Print. Mass-spectrometry (MS)-based proteomics is a powerful and robust platform for studying the interactions between biological systems during health and disease. Bacterial infections represent a significant threat to global health and drive the pursuit of novel therapeutic strategies to combat emerging and resistant pathogens. During infection, the interplay between a host and pathogen determines the ability of the microbe to survive in a hostile environment and promotes an immune response by the host as a protective measure. It is the protein-level changes from either biologic...
Source: Canadian Journal of Microbiology - October 7, 2020 Category: Microbiology Authors: Arjun Sukumaran Elizabeth Woroszchuk Taylor Ross Jennifer Geddes-McAlister Source Type: research
Long-term nitrogen fertilization shaped the nifH, nirK, and nosZ gene community patterns in red paddy soil in south China
Canadian Journal of Microbiology, Ahead of Print. To understand the diversities of diazotrophs and denitrifiers in red paddy soil under long-term fertilization conditions, nifH, nirK, and nosZ libraries were constructed by PCR –RFLP. nirK gene diversity proved to be lower than that of nosZ and nifH, and nirK and nosZ genes were more sensitive to different fertilization treatments than the nifH gene was. The 3 libraries were dominated by diverse microbes, including the Alpha, Beta, Gamma, and Delta subclasses of the Prot eobacteria. Long-term addition of urea with straw mulch and azophoska increased the abundance of nonsy...
Source: Canadian Journal of Microbiology - October 6, 2020 Category: Microbiology Authors: Cunzhi Zhang Kaixun Cao Yue Li Juan Zhao Wentao Peng Hui Cao Xin Xiao Source Type: research
Diversity and community structure of ammonia-oxidizing archaea in rhizosphere soil of four plant groups in Ebinur Lake wetland
Canadian Journal of Microbiology, Ahead of Print. The aim of this study was to reveal the differences in the community structure of ammonia-oxidizing archaea (AOA) between rhizosphere and non-rhizosphere soil, to provide a theoretical basis for further study on the relationship between halophyte rhizosphere soil microorganisms and salt tolerance. The results of diversity and community structure showed that the diversity of the AOA community in rhizosphere soil of Reeds was higher than that in non-rhizosphere soil in spring and lower than that in non-rhizosphere soil in summer and autumn. In summer, the diversity of rhizosp...
Source: Canadian Journal of Microbiology - September 29, 2020 Category: Microbiology Authors: Ting Chen Wenge Hu Shuaibing He Xue Zhang Yanhui Niu Source Type: research
Inactivation of Salmonella Enteritidis on cherry tomatoes by ultrasound, lactic acid, detergent, and silver nanoparticles
This study aimed to evaluate the use of US (40 kHz for 5 min) alone or with 1% lactic acid (LA), 1% commercial detergent (DET), or 6 mg/L silver nanoparticles (AgNP, average diameter 100 nm) as an alternative treatment to 200 mg/L sodium dichloroisocyanurate for inactivating Salmonella enterica serovar Enteritidis present on cherry tomatoe s. The interfacial tension between sanitizing solutions and bacterial adhesion was investigated. Sanitizers in solutions with DET and AgNP had lower surface tension. All treatments, except that with DET, reduced Salmonella Enteritidis by more than one logarithmic cycle. There was n...
Source: Canadian Journal of Microbiology - September 21, 2020 Category: Microbiology Authors: Jackline Freitas Brilhante de S ão José Afonso Mota Ramos Maria Cristina Dantas Vanetti N élio José de Andrade Source Type: research
Delineating the key virulence factors and intraspecies divergence of Vibrio harveyi via whole-genome sequencing
In conclusion, the results indicated that the virulence level of V. harveyi is mainly determined by the above virulence genes, which may play vital roles in environmental adaptation for V. harveyi. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - September 17, 2020 Category: Microbiology Authors: Songzhe Fu Ping Ni Qian Yang Huizhi Hu Qingyao Wang Shigen Ye Ying Liu Source Type: research
Crop, genotype, and field environmental conditions shape bacterial and fungal seed epiphytic microbiomes
In this study, wheat, canola, and lentil seeds were analyzed to characterize diversity, structure, and persistence of seed-associated microbial communities. Five lines and 2 generations of each crop were subjected to high-throughput amplicon sequencing of the 16S rRNA and internal transcribed spacer (ITS) regions. Bacterial and fungal communities differed most by crop type (30% and 47% of the variance), while generation explained an additional 10% and 15% of the variance. The offspring (i.e., generation harvested in 2016 at the same location) exhibited a h igher number of common amplicon sequence variants (ASVs) and less...
Source: Canadian Journal of Microbiology - September 15, 2020 Category: Microbiology Authors: Zayda P. Morales Moreira Bobbi L. Helgason James J. Germida Source Type: research
Development of droplet digital PCR assays to quantify genes involved in nitrification and denitrification, comparison with quantitative real-time PCR and validation of assays in vineyard soil
This study optimized eight ddPCR assays to quantify total bacteria and archaea as well as the nitrification (bacterial and archaeal amoA) and denitrification (nirS, nirK, nosZI, nosZII) genes involved in the generation or reduction of the greenhouse gas nitrous oxide. Detection and quantification thresholds were compared with those of quantitative real-time PCR and were equal to, or improved, in ddPCR. To validate the assays using environmental samples, soil DNA was isolated from two vineyards in the Okanagan valley in British Columbia, Canada, over the 2017 growing season. Soil properties related to the observed gene abun...
Source: Canadian Journal of Microbiology - September 10, 2020 Category: Microbiology Authors: Tanja M. Voegel Melissa M. Larrabee Louise M. Nelson Source Type: research
Phosphate efflux as a test of plasma membrane leakage in Saccharomyces cerevisiae cells
Canadian Journal of Microbiology, Ahead of Print. Plasma membrane integrity is a key to cell viability. Currently, the main approach to assessing plasma membrane integrity is the detection of penetration of special dyes, such as trypan blue and propidium iodide, into the cells. However, this method needs expensive equipment: a fluorescent microscope or a flow cytometer. Besides, staining with propidium iodide occasionally gives false-positive results. Here, we suggest the phosphate (Pi) leakage assay as an approach to assess the increase in permeability of the plasma membrane of yeast cells. We studied the dependence of ph...
Source: Canadian Journal of Microbiology - September 10, 2020 Category: Microbiology Authors: Ludmila Trilisenko Airat Valiakhmetov Tatiana Kulakovskaya Source Type: research
