Uneven genotypic diversity of Escherichia coli in fecal sources limits the performance of a library-dependent method of microbial source tracking on the southwestern French Atlantic coast
Canadian Journal of Microbiology, e-First Articles. To develop a library-dependent method of tracking fecal sources of contamination of beaches on the Atlantic coast of southwestern France, a library of 6368 Escherichia coli isolates was constructed from samples of feces, from 40 known human or animal sources collected in the vicinity of Arcachon Bay in 2010, and in French Basque Country, Landes, and B éarn, between 2017 and 2018. Different schemes of source identification were tested: use of the complete or filtered reference library; characterization of the isolates by genotypic or proteomic profiling based on ERIC-PCR ...
Source: Canadian Journal of Microbiology - July 29, 2020 Category: Microbiology Authors: Fr édéric Garabetian Isabelle Vitte Antoine Sabourin H élène Moussard Adeline Jouanillou Line Mornet M élanie Lesne Emilie Lyautey Source Type: research
Aluminum induces oxidative damage in Saccharomyces cerevisiae
Canadian Journal of Microbiology, e-First Articles. The mechanism of aluminum toxicity was studied in the model cells of Saccharomyces cerevisiae. Cell growth of yeast was inhibited by aluminum. The spot assay showed that the mechanism of aluminum detoxification in yeast cells was different from that of heavy metal cadmium. After treatment with aluminum, intracellular levels of reactive oxygen species, protein carbonyl, and thiobarbituric acid reactive substances were dramatically increased. Meanwhile, the percentage of aluminum-treated cells permeable to propidium iodide was augmented significantly. These data demonstrate...
Source: Canadian Journal of Microbiology - July 29, 2020 Category: Microbiology Authors: Ranran Chen Qian Zhu Zhijia Fang Zhiwei Huang Jing Sun Min Peng Ping Shi Source Type: research
Finding the silver lining during a global pandemic: opportunities for curriculum innovation in microbiology education
Canadian Journal of Microbiology, e-First Articles. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - July 29, 2020 Category: Microbiology Authors: Maria C. Davis Josie Libertucci Yanelis Acebo Guerrero Heather Dietz Tanya C. Noel Joseph E. Rubin Nicole Sukdeo Source Type: research
Exposure of Agrobacterium tumefaciens to agroinfiltration medium demonstrates cellular remodelling and may promote enhanced adaptability for molecular pharming
Canadian Journal of Microbiology, Ahead of Print. Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for industrial-scale protein production exposes bacteria (harboring genes of interest) to agroinfiltration medium that is devoid of nutrients and carbon sources for prolonged periods of time (possibly upwards of 24 h). Such conditions may negatively influence bact...
Source: Canadian Journal of Microbiology - July 27, 2020 Category: Microbiology Authors: N. Prudhomme R. Pastora B. Muselius M.D. McLean D. Cossar J. Geddes-McAlister Source Type: research
Exposure of Agrobacterium tumefaciens to agroinfiltration medium demonstrates cellular remodelling and may promote enhanced adaptability for molecular pharming
Canadian Journal of Microbiology, e-First Articles. Agroinfiltration is used to treat plants with modified strains of Agrobacterium tumefaciens for the purpose of transient in planta expression of genes transferred from the bacterium. These genes encode valuable recombinant proteins for therapeutic or industrial applications. Treatment of large quantities of plants for industrial-scale protein production exposes bacteria (harboring genes of interest) to agroinfiltration medium that is devoid of nutrients and carbon sources for prolonged periods of time (possibly upwards of 24 h). Such conditions may negatively influence ba...
Source: Canadian Journal of Microbiology - July 27, 2020 Category: Microbiology Authors: N. Prudhomme R. Pastora B. Muselius M.D. McLean D. Cossar J. Geddes-McAlister Source Type: research
The Canadian Fungal Research Network: current challenges and future opportunities
Canadian Journal of Microbiology, Ahead of Print. Fungi critically impact the health and function of global ecosystems and economies. In Canada, fungal researchers often work within silos defined by subdiscipline and institutional type, complicating the collaborations necessary to understand the impacts fungi have on the environment, economy, and plant and animal health. Here, we announce the establishment of the Canadian Fungal Research Network (CanFunNet, https://fungalresearch.ca), whose mission is to strengthen and promote fungal research in Canada by facilitating dialogue among scientists. We summarize the challenges ...
Source: Canadian Journal of Microbiology - July 26, 2020 Category: Microbiology Authors: Linda C. Horianopoulos Emile Gluck-Thaler Isabelle Benoit Gelber Leah E. Cowen Jennifer Geddes-McAlister Christian R. Landry Ilan S. Schwartz James A. Scott Adnane Sellam Donald C. Sheppard Toby Spribille Rajagopal Subramaniam Allison K. Walker Steven D. Source Type: research
Functional potential differences between Firmicutes and Proteobacteria in response to manure amendment in a reclaimed soil
Canadian Journal of Microbiology, Ahead of Print. Manure amendment generally bolsters soil organisms but not all bacteria equally. To understand why different taxa respond differently, we used shotgun metagenomic approaches to profile functional potentials and correlate them with taxon abundances. A soil originally unproductive was reclaimed using commercial manure and finally became productive. The abundance of Firmicutes in the soil decreased, whereas that of Bacteroidetes and Proteobacteria increased after manure addition. Thirty-nine KEGG modules were significantly different across fertilizer treatments. These modules ...
Source: Canadian Journal of Microbiology - July 26, 2020 Category: Microbiology Authors: Wenxi Li Yueping Zhang Wei Mao Changsong Wang Shixue Yin Source Type: research
The Canadian Fungal Research Network: current challenges and future opportunities
Canadian Journal of Microbiology, Ahead of Print. Fungi critically impact the health and function of global ecosystems and economies. In Canada, fungal researchers often work within silos defined by subdiscipline and institutional type, complicating the collaborations necessary to understand the impacts fungi have on the environment, economy, and plant and animal health. Here, we announce the establishment of the Canadian Fungal Research Network (CanFunNet, https://fungalresearch.ca), whose mission is to strengthen and promote fungal research in Canada by facilitating dialogue among scientists. We summarize the challenges ...
Source: Canadian Journal of Microbiology - July 26, 2020 Category: Microbiology Authors: Linda C. Horianopoulos Emile Gluck-Thaler Isabelle Benoit Gelber Leah E. Cowen Jennifer Geddes-McAlister Christian R. Landry Ilan S. Schwartz James A. Scott Adnane Sellam Donald C. Sheppard Toby Spribille Rajagopal Subramaniam Allison K. Walker Steven D. Source Type: research
Functional potential differences between Firmicutes and Proteobacteria in response to manure amendment in a reclaimed soil
Canadian Journal of Microbiology, Ahead of Print. Manure amendment generally bolsters soil organisms but not all bacteria equally. To understand why different taxa respond differently, we used shotgun metagenomic approaches to profile functional potentials and correlate them with taxon abundances. A soil originally unproductive was reclaimed using commercial manure and finally became productive. The abundance of Firmicutes in the soil decreased, whereas that of Bacteroidetes and Proteobacteria increased after manure addition. Thirty-nine KEGG modules were significantly different across fertilizer treatments. These modules ...
Source: Canadian Journal of Microbiology - July 26, 2020 Category: Microbiology Authors: Wenxi Li Yueping Zhang Wei Mao Changsong Wang Shixue Yin Source Type: research
The Canadian Fungal Research Network: current challenges and future opportunities
Canadian Journal of Microbiology, e-First Articles. Fungi critically impact the health and function of global ecosystems and economies. In Canada, fungal researchers often work within silos defined by subdiscipline and institutional type, complicating the collaborations necessary to understand the impacts fungi have on the environment, economy, and plant and animal health. Here, we announce the establishment of the Canadian Fungal Research Network (CanFunNet, https://fungalresearch.ca), whose mission is to strengthen and promote fungal research in Canada by facilitating dialogue among scientists. We summarize the challenge...
Source: Canadian Journal of Microbiology - July 26, 2020 Category: Microbiology Authors: Linda C. Horianopoulos Emile Gluck-Thaler Isabelle Benoit Gelber Leah E. Cowen Jennifer Geddes-McAlister Christian R. Landry Ilan S. Schwartz James A. Scott Adnane Sellam Donald C. Sheppard Toby Spribille Rajagopal Subramaniam Allison K. Walker Steven D. Source Type: research
Functional potential differences between Firmicutes and Proteobacteria in response to manure amendment in a reclaimed soil
Canadian Journal of Microbiology, e-First Articles. Manure amendment generally bolsters soil organisms but not all bacteria equally. To understand why different taxa respond differently, we used shotgun metagenomic approaches to profile functional potentials and correlate them with taxon abundances. A soil originally unproductive was reclaimed using commercial manure and finally became productive. The abundance of Firmicutes in the soil decreased, whereas that of Bacteroidetes and Proteobacteria increased after manure addition. Thirty-nine KEGG modules were significantly different across fertilizer treatments. These module...
Source: Canadian Journal of Microbiology - July 26, 2020 Category: Microbiology Authors: Wenxi Li Yueping Zhang Wei Mao Changsong Wang Shixue Yin Source Type: research
Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters
Canadian Journal of Microbiology, Ahead of Print. We investigated the specificity and sensitivity of two horse-associated markers, HoF597 and Horse mtCytb, and 12 mitochondrial and bacterial markers of six animal species (human, cow, pig, bird, dog, chicken) in the faecal samples of 50 individual horses. Both horse markers were detected in 48 (96%) faecal samples. Cross-reactivity with dog (BacCan545) and pig (P23-2) occurred in 88% and 72% of horse faecal samples, respectively. Several other bacterial and mitochondrial markers of non-target hosts were also detected; however, their specificities were>80%. Analyses of sa...
Source: Canadian Journal of Microbiology - July 20, 2020 Category: Microbiology Authors: Jessica Gray Nicole Masters Aaron Wiegand Mohammad Katouli Source Type: research
Field assessment of horse-associated genetic markers HoF597 and mtCytb for detecting the source of contamination in surface waters
Canadian Journal of Microbiology, e-First Articles. We investigated the specificity and sensitivity of two horse-associated markers, HoF597 and Horse mtCytb, and 12 mitochondrial and bacterial markers of six animal species (human, cow, pig, bird, dog, chicken) in the faecal samples of 50 individual horses. Both horse markers were detected in 48 (96%) faecal samples. Cross-reactivity with dog (BacCan545) and pig (P23-2) occurred in 88% and 72% of horse faecal samples, respectively. Several other bacterial and mitochondrial markers of non-target hosts were also detected; however, their specificities were>80%. Analyses of ...
Source: Canadian Journal of Microbiology - July 20, 2020 Category: Microbiology Authors: Jessica Gray Nicole Masters Aaron Wiegand Mohammad Katouli Source Type: research
