Analysis of biofilm expansion rate of Bacillus subtilis (MTC871) on agar substrates with different stiffness
Canadian Journal of Microbiology, Ahead of Print. The surface morphology of mature biofilms is heterogeneous and can be divided into concentric rings wrinkles (I), labyrinthine networks wrinkles (II), radial ridges wrinkles (III), and branches wrinkles (IV), according to surface wrinkle structure and distribution characteristics. Due to the wrinkle structures, channels are formed between the biofilm and substrate and transport nutrients, water, metabolic products, etc. We find that expansion rate variations of biofilms growing on substrates with high and low agar concentrations (1.5, 2.0, 2.5 wt.%) are not in the same p...
Source: Canadian Journal of Microbiology - June 28, 2023 Category: Microbiology Authors: Jin Wu Xianyong Li Rui Kong Jiankun Wang Xiaoling Wang Source Type: research
Bacterial community structure in the rhizosphere of fungi-infected Amorphophallus titanum
Canadian Journal of Microbiology, Ahead of Print. The rhizosphere is a narrow soil area directly affected by plant root exudates. Microbes inhabiting the rhizosphere have been widely studied for their beneficial effects on plant nutrition, growth, and disease prevention. Many factors affect the rhizosphere microbial composition, including plant pathogen infection. Here, we analyzed the bacterial community structure in the rhizosphere of fungi-infected Amorphophallus titanum. Soil samples were collected from rhizosphere and non-rhizosphere areas of fungi-infected A. titanum. The 16S metagenomic analysis was conducted to inv...
Source: Canadian Journal of Microbiology - June 26, 2023 Category: Microbiology Authors: Sipriyadi Sipriyadi Yeni Khairina Masrukhin Masrukhin Adi Yulandi Risky Hadi Wibowo Dhiatama Tauhida Nisa Source Type: research
Polymerase chain reaction-based typing methods and protein profiling analysis of Acinetobacter baumannii isolated from environmental and clinical sources from South India
Canadian Journal of Microbiology, Ahead of Print. Acinetobacter baumannii is an opportunistic pathogen known for causing hospital-acquired infections. The natural habitat includes soil, water, sewage, and drains, but it is also detected in infected individuals ’ blood, pus, and respiratory pathways. Due to its resilient nature, it is known to be a causative agent for outbreaks. Therefore, it is crucial to understand the genetic similarity between clinical and environmental isolates. The study aimed to find the genetic relationships between clinical and environmental isolates using PCR-based typing methods such as enterob...
Source: Canadian Journal of Microbiology - June 26, 2023 Category: Microbiology Authors: Srajana Nayak D ’Almeida Aanice Dsouza Andria Archana Pai Biswajit Maiti Source Type: research
Characterization of an EPS-producing bifidobacterial strain based on integration of phenotypic and complete genome sequencing data
In this study, 21 strains belonging to Bifidobacterium and Lactobacillus were isolated from healthy infants ’ stool and were screened for EPS-producing ability. Among these strains, Bifidobacterium longum XZM1 showed the highest EPS productivity, which was further confirmed and characterized. The complete genome of strain XZM1 was sequenced, which revealed the presence of a gene cluster for EPS producti on. Furthermore, comparative genome analysis was performed among XZM1 and other strains from B. longum species. Following purification, the molecular weight (Mw) of EPS from XZM1 was determined as 4023 Da (Mw) through ge...
Source: Canadian Journal of Microbiology - June 23, 2023 Category: Microbiology Authors: Dianbin Liu Haohan Huang Jinzhi Han Qiong Wu Yaoyao Xiang Yan Liu Yanxia Wei Source Type: research
The VxrAB two-component system is important for the polymyxin B-dependent activation of the type VI secretion system in Vibrio cholerae O1 strain A1552
Canadian Journal of Microbiology, Ahead of Print. The type VI secretion system (T6SS) is used by bacteria for virulence, resistance to grazing, and competition with other bacteria. We previously demonstrated that the role of the T6SS in interbacterial competition and in resistance to grazing is enhanced in Vibrio cholerae in the presence of subinhibitory concentrations of polymyxin B. Here, we performed a global quantitative proteomic analysis and a targeted transcriptomic analysis of the T6SS-known regulators in V. cholerae grown with and without polymyxin B. The proteome of V. cholerae is greatly modified by polymyxin B ...
Source: Canadian Journal of Microbiology - June 21, 2023 Category: Microbiology Authors: Annabelle Mathieu-Denoncourt Marylise Duperthuy Source Type: research
Potential selection and maintenance of manure-originated multi-drug resistant plasmids at sub-clinical concentrations for tetracycline family antibiotics
This study used two plasmids (pT295A and pT413A) originating from dairy manure in a surrogate Escherichia coli host CV601. The minimum selection concentrations of antibiotics tested in nutrient-rich medium were determined as follows: 0.1 mg/L for oxytetracycline, 0.45 mg/L for chlortetracycline, and 0.13–0.25 mg/L for tetracycline. Mixing oxytetracycline and chlortetracycline had minimum selection concentration values increased 2-fold compared to those in single antibiotic tests. Minimum selection concentrations found in th is study were lower than reported environmental hotspot concentrations, suggesting that tet...
Source: Canadian Journal of Microbiology - June 2, 2023 Category: Microbiology Authors: Tam T. Tran Marlena Cole Emily Tomas Andrew Scott Edward Topp Source Type: research
The scientific revolution that unraveled the astonishing DNA repair capacity of the Deinococcaceae: 40 years on
Canadian Journal of Microbiology, Ahead of Print. The family Deinococcaceae exhibits exceptional radiation resistance and possesses all the necessary traits for surviving in radiation-exposed environments. Their survival strategy involves the coupling of metabolic and DNA repair functions, resulting in an extraordinarily efficient homologous repair of DNA double-strand breaks (DSBs) caused by radiation or desiccation. The keys to their survival lie in the hyperaccumulation of manganous (Mn2+)-metabolite antioxidants that protect their DNA repair proteins under extreme oxidative stress and the persistent structural linkage ...
Source: Canadian Journal of Microbiology - June 2, 2023 Category: Microbiology Authors: Michael J. Daly Source Type: research
New insights into the composition and diversity of endophytic bacteria in cultivated Huperzia serrata
In this study, the composition and diversity of endophytic bacteria in the roots, stems, and leaves from 3-year-old artificially cultivated Huperzia serrata were investigated using Illumina HiSeq sequencing technology. Total effective reads were assigned to 936 operational taxonomic units (OTUs), belonging to 12 phyla and 289 genera. A total of 28, 3, and 2 OTUs were exclusive to the roots, stems, and leaves, respectively. The bacterial richness and diversity in the roots were significantly lower than those in the leaves and stems. The dominant genera with significant distribution differences among these plant tissue sampl...
Source: Canadian Journal of Microbiology - May 30, 2023 Category: Microbiology Authors: Wen-Jing Pan Li-Yun Miao Shi-Peng Fan Pin-Wei Lv Ai-Hua Lin Hong Geng Fa-Jun Song Peng Zhang Source Type: research
Biodegradation of 2-methylpentane in fluid fine tailings amended with a mixture of iso-alkanes under sulfate-reducing conditions
This study underlines the limited capability of the microbial community indigenous to CNUL FFT in degrading recalcitrant iso-alkanes under sulfa te-reducing conditions. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - May 26, 2023 Category: Microbiology Authors: Mohd Faidz Mohamad Shahimin Tariq Siddique Source Type: research
Gentamicin resistance to Escherichia coli related to fatty acid metabolism based on transcriptome analysis
In this study, we explored the mechanism of gentamicin resistance by comparing the transcriptomes of antibiotic-sensitive and -resistant Escherichia coli. A total of 410 differentially expressed genes were identified, of which 233 (56.83%) were up-regulated and 177 (43.17%) were down-regulated in the resistant strain compared with the sensitive strain. Gene Ontology (GO) analysis classifies differential gene expression into three main categories: biological processes, cellular components, and molecular functions. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the up-regulated genes were enri...
Source: Canadian Journal of Microbiology - May 24, 2023 Category: Microbiology Authors: Zhiyu Pan Juan Guo Yilin Zhong Lvyuan Fan Yubin Su Source Type: research
Distribution of corrosive microbial communities in rust layers of steel immersed in seawater
Canadian Journal of Microbiology, Ahead of Print. In this paper, high-throughput sequencing technology was adopted to visualize the microbial communities on the surfaces of two types of carbon steel immersed in Sea Area Ⅰ. The results showed that different microbial communities were formed on different carbon steel surfaces, in which the genus with the highest abundance on the surface of Q235 was Escherichia–Shigella, while anaerobic Desulfovibrio on the surface of 921a was the most abundant, and the dominant g enus varied with the depth of the rust layer. In addition, the distribution of sulfate-reducing bacteria (SRB...
Source: Canadian Journal of Microbiology - May 8, 2023 Category: Microbiology Authors: Qijing Ren Yimeng Zhang Jizhou Duan Wangqiang Li Xucheng Dong Yingying Pei Ruiyong Zhang Qingjun Zhu Source Type: research
A graduate student's tribute to Dr. R.G.E. Murray
Canadian Journal of Microbiology, Ahead of Print. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - April 28, 2023 Category: Microbiology Authors: Ronald S. Flannagan Source Type: research
An endophytic fungus Schizophyllum commune isolated from Panax ginseng enhances hairy roots growth and ginsenoside biosynthesis
In conclusion, this study is the first to show that the EM of endophytic fungus S. commune can be considered as an effective endophytic fungal elicitor for increasing the biosynthesis of ginsenosides in hairy root cultures of P. ginseng. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - April 21, 2023 Category: Microbiology Authors: Xing-Guang Xie Zhen-Zhen Zhang Ling Chen Qian-Liang Ming Ke-Xin Sheng Xi Chen Khalid Rahman Kun-Miao Feng Juan Su Ting Han Source Type: research
Dr. RGE Murray: scientist, teacher, and mentor
Canadian Journal of Microbiology, Ahead of Print. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - April 14, 2023 Category: Microbiology Authors: Sharon Walmsley Source Type: research
Analysis of bacterioplankton genes in an impaired Great Lakes harbour reveals seasonal metabolic shifts and a previously undetected cyanobacterium
Canadian Journal of Microbiology, Ahead of Print. Hamilton Harbour is an impaired embayment of Lake Ontario that experiences seasonal algal blooms despite decades of remedial efforts. To study the harbour ’s cyanobacterial and heterotrophic bacterial communities, we extracted and sequenced community DNA from surface water samples collected biweekly from different sites during summer and fall. Assembled contigs were annotated at the phylum level, and Cyanobacteria were further characterized at order and species levels. Actinobacteria were most abundant in early summer, while Cyanobacteria were dominant in mid-summer. Micr...
Source: Canadian Journal of Microbiology - April 13, 2023 Category: Microbiology Authors: Christine N. Palermo Roberta R. Fulthorpe Rosemary Saati Steven M. Short Source Type: research
