Wild fish from a highly urbanized river (Orge, France) as vectors of culturable Enterobacterales resistant to antibiotics
This study shows how wild fishes from urbanized rivers could be involved in the spread of antibiotic-resistant Enterobacterales. Antibiotic resistance profiles and molecular detection of clinical integron (IntI1) were carried out on 105 Enterobacterales isolated from 89 wildfish (skin or gut) belonging to 8 species. The proportion of isolates resistant to at least one antibiotic was independent of fish species and reached 28.3% within the Escherichia coli (E. coli) population and 84.7% in the non-E.coli Enterobacterales. Bacteria involved in nosocomial infections were isolated, such as E. coli, Klebsiella, and Enterobacter...
Source: Canadian Journal of Microbiology - December 8, 2023 Category: Microbiology Authors: Anne-Laure Vivant Etienne Marchand Benjamin Janvier Thierry Berthe Elodie Guigon Nathalie Grall Fabrice Alliot Aur élie Goutte Fabienne Petit Source Type: research
Genomic characterization of three bacterial isolates antagonistic to the pea root rot pathogen Aphanomyces euteiches
In this study, the genomes of three isolates —Pseudomonas sp. rhizo 66 (PD-S66), Pseudomonas synxantha rhizo 25 (Ps-S25), and Serratia sp. root 2 (TS-R2)—were sequenced, assembled, and annotated. Genome size of PD-S66 was 6 279 416 bp with 65 contigs, 59.32% GC content, and 5653 predicted coding sequences (CDS). Genome size of Ps-S25 was 6 058 437 bp with 66 contigs, a GC content of 60.08%, and 5575 predicted CDS. The genome size of TS-R2 was 5 282 152 bp, containing 26 contigs, a GC content of 56.17%, and 4956 predicted CDS. For the identification of the isolates, digital DNA–DNA hybridization (dDDH) ...
Source: Canadian Journal of Microbiology - December 7, 2023 Category: Microbiology Authors: Zakir Hossain Michelle Hubbard Source Type: research
Time to treat the climate and nature crisis as one indivisible global health emergency
Canadian Journal of Microbiology,Volume 69, Issue 12, Page i-iii, December 2023. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - December 1, 2023 Category: Microbiology Source Type: research
Role of an antagonistic bacterium, Bacillus subtilis PE7, in growth promotion of netted melon (Cucumis melo L. var. reticulatus Naud.)
Canadian Journal of Microbiology, Ahead of Print. The aim of this study was to determine the plant growth-promoting effect of Bacillus subtilis PE7 on growth of melon plants. B. subtilis PE7 isolated from kimchi was identified based on colonial and microscopic morphology along with analyses of 16S rRNA and pycA gene sequences. Strain PE7 showed different levels of inhibition on phytopathogens and was able to grow at variable temperatures and pH values. Strain PE7 had the ability to produce siderophores, indole-3-acetic acid (IAA), ammonia, exopolysaccharides, and 1-aminocyclopropane-1-carboxylic acid deaminase, as well as ...
Source: Canadian Journal of Microbiology - November 2, 2023 Category: Microbiology Authors: Seong Eun Han Jeong-Yong Cho Kil Yong Kim Chaw Ei Htwe Maung Source Type: research
Proteomic profile of Toxoplasma gondii stress granules by high-resolution mass spectrometry
Canadian Journal of Microbiology, Ahead of Print. Ribonucleoprotein granules are bio-condensates that form a diverse group of dynamic membrane-less organelles implicated in several cellular functions, including stress response and cellular survival. In Toxoplasma gondii, a type of bio-condensates referred to as stress granules (SGs) are formed prior to the parasites ’ egress from the host cell and are implicated in the survival and invasion competency of extracellular tachyzoites. We used paraformaldehyde to fix and cross-link SG proteins to allow purification by centrifugation and analysis by mass spectrometry. We profi...
Source: Canadian Journal of Microbiology - October 12, 2023 Category: Microbiology Authors: Scott Roscoe Yue Guo Panayiotis O. Vacratsis Sirinart Ananvoranich Source Type: research
Virulence-associated genes and antimicrobial resistance patterns in bacteria isolated from pregnant and nonpregnant women with urinary tract infections: the risk of neonatal sepsis
In conclusion, UPEC isolated from nonpregnant women carried more virulence factors than those isolated from pregnant women, and maternal UTIs were associated with neonatal sepsis risk. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - October 10, 2023 Category: Microbiology Authors: Uriel A. Angulo-Zamudio Hector Flores-Villase ñor Nidia Leon-Sicairos Dina Zazueta-Armenta Francisco A. Mart ínez-Villa Gabriela Tapia-Pastrana Jorge Angulo-Rocha Joel Murillo-Llanes Mario Francisco Barajas-Olivas Adrian Canizalez-Roman Source Type: research
Influence of central metabolism disruption on Escherichia coli biofilm formation
Canadian Journal of Microbiology, Ahead of Print. Biofilms are widely recognized as a prominent mode of microbial growth and strategy of antimicrobial tolerance in many environments. Characteristics that are often overlooked in biofilm investigations include the examination of metabolic pathways as the assumption might be that interference with central pathways such as glycolysis would only reduce growth and thus not be meaningful. Using the Keio collection of Escherichia coli mutants, we investigated the influence of biofilm formation and planktonic growth in full-strength and diluted Luria –Bertani (LB) broths using st...
Source: Canadian Journal of Microbiology - September 20, 2023 Category: Microbiology Authors: Taylor M. Ranson Marilynn E. Barton Robert J.C. McLean Source Type: research
Correction: The scientific revolution that unraveled the astonishing DNA repair capacity of the Deinococcaceae: 40 years on
Canadian Journal of Microbiology, Ahead of Print. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - September 20, 2023 Category: Microbiology Authors: Michael J. Daly Source Type: research
Salicylic acids and pathogenic bacteria: new perspectives on an old compound
Canadian Journal of Microbiology, Ahead of Print. Salicylic acids have been used in human and veterinary medicine for their anti-pyretic, anti-inflammatory, and analgesic properties for centuries. A key role of salicylic acid —immune modulation in response to microbial infection—was first recognized during studies of their botanical origin. The effects of salicylic acid on bacterial physiology are diverse. In many cases, they impose selective pressures leading to development of cross-resistance to antimicrobial compo unds. Initial characterization of these interactions was in Escherichia coli, where salicylic acid acti...
Source: Canadian Journal of Microbiology - September 12, 2023 Category: Microbiology Authors: Ellen M.E. Sykes Dawn White Sydney McLaughlin Ayush Kumar Source Type: research
Pseudomonas mosselii improves cold tolerance of Asian rice (Oryza sativa L.) in a genotype-dependent manner by increasing proline in japonica and reduced glutathione in indica varieties
This study confirmed the host genotype dependent activity of P. mosselii and indicated that there is an interaction between specific plant genes and bacterial genes that causes different plant responses to cold stress. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - September 12, 2023 Category: Microbiology Authors: Nasim Maghboli Balasjin James S. Maki Michael R. Schl äppi Source Type: research
Phosphatidylinositol-specific phospholipase C can decrease M üller cell viability and suppress its phagocytic activity by modulating PI3K/AKT signaling pathway
In conclusion, we showed that PI-PLC proteins contribute to inhibit the viability of and suppress the phagocytosis of Müller c ells, providing new insights into the pathogenic mechanism of B. cereus endophthalmitis. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - September 6, 2023 Category: Microbiology Authors: Bianjin Sun Shudan Lin Mengmeng Zheng Beijia Zheng Liping Mao Yunfeng Gu Jiabei Cai Yiran Dai Meiqin Zheng Yongliang Lou Source Type: research
Update on the genus Robertmurraya: a bacterial genus honoring Dr. Robert G.E. Murray (with some personal reminiscences)
This article also reflects on the serendipitous way I came to know Dr. Murray and his extensive interactions with me and strong support for our work for more tha n 10 years. Dr. Murray also introduced me and our work to his friend and contemporary Dr. Peter Sneath, who like him also contributed extensively to the field of prokaryotic taxonomy. This introduction led to a fruitful collaboration with Dr. Sneath leading to a joint publication describing the use of the Character Compatibility approach to molecular sequence data. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - August 9, 2023 Category: Microbiology Authors: Radhey S. Gupta Source Type: research
DNA repair and oxidative stress defense systems in radiation-resistant Deinococcus murrayi
Canadian Journal of Microbiology, Ahead of Print. Deinococcus murrayi is a bacterium isolated from hot springs in Portugal, and named after Dr. Robert G.E. Murray in recognition of his research on the genus Deinococcus. Like other Deinococcus species, D. murrayi is extremely resistant to ionizing radiation. Repair of massive DNA damage and limitation of oxidative protein damage are two important factors contributing to the robustness of Deinococcus bacteria. Here, we identify, among others, the DNA repair and oxidative stress defense proteins in D. murrayi, and highlight special features of D. murrayi. For DNA repair, D. m...
Source: Canadian Journal of Microbiology - August 8, 2023 Category: Microbiology Authors: Arjan de Groot Laurence Blanchard Source Type: research
Effects of short-term exposure to simulated microgravity on the physiology of Bacillus subtilis and multiomic analysis
Canadian Journal of Microbiology, Ahead of Print. In our study, Bacillus subtilis was disposed to a simulated microgravity (SMG) environment in high-aspect ratio rotating-wall vessel bioreactors for 14 days, while the control group was disposed to the same bioreactors in a normal gravity (NG) environment for 14 days. The B. subtilis strain exposed to the SMG (labeled BSS) show ed an enhanced growth ability, increased biofilm formation ability, increased sensitivity to ampicillin sulbactam and cefotaxime, and some metabolic alterations compared with the B. subtilis strain under NG conditions (labeled BSN) and the origi...
Source: Canadian Journal of Microbiology - July 18, 2023 Category: Microbiology Authors: Xiaolei Su Tingzheng Fang Lin Fang Dapeng Wang Xuege Jiang Changting Liu Honglei Zhang Rui Guo Junfeng Wang Source Type: research
New insights into endophytic fungi diversity and their potential correlation with polyphyllin levels of Paris polyphylla var. yunnanensis
This study provides valuable information for further research of the ecological and bi ological functions of endophytic fungi of P. polyphylla. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - July 10, 2023 Category: Microbiology Authors: Juan Lan Ting Liu Liyun Miao Ting Pei Zhe Gan Aihua Lin Hong Geng Peng Zhang Source Type: research
