Characterization of a plant-growth-promoting non-nodulating endophytic bacterium (Stenotrophomonas maltophilia) from the root nodules of Mucuna utilis var. capitata L. (Safed Kaunch)
In this study, a nonrhizobial endophytic bacterial strain was isolated from the root nodules of the medicinal legume Mucuna utilis var. capitata L.; phenotypic, genotypic, and agricultural characterization was performed using a HiMedia kit and 16S rRNA sequencing. This strain showed tremendous seedling growth potential (30%), compared with the control, as well as a strong antagonistic nature against the plant pathogenic fungus Fusarium udum when plant growth parameters were analyzed. The strain, identified by 16S rRNA as Stenotrophomonas maltophilia, showed a multitude of plant-growth-promoting attributes both direct (IAA,...
Source: Canadian Journal of Microbiology - July 7, 2020 Category: Microbiology Authors: Abhinav Aeron Ramesh Chandra Dubey Dinesh Kumar Maheshwari Source Type: research

Potential impact of SARS-CoV-2 infection on the thymus
Canadian Journal of Microbiology, Ahead of Print. Understanding the pathogenesis of certain viral agents is essential for developing new treatments and obtaining a clinical cure. With the onset of the new coronavirus (SARS-CoV-2) pandemic in the beginning of 2020, a rush to conduct studies and develop drugs has led to the publication of articles that seek to address knowledge gaps and contribute to the global scientific research community. There are still no reports on the infectivity or repercussions of SARS-CoV-2 infection on the central lymphoid organ, the thymus, nor on thymocytes or thymic epithelial cells. In this br...
Source: Canadian Journal of Microbiology - July 7, 2020 Category: Microbiology Authors: Marvin Paulo Lins Salete Smaniotto Source Type: research

Characterization of a plant-growth-promoting non-nodulating endophytic bacterium (Stenotrophomonas maltophilia) from the root nodules of Mucuna utilis var. capitata L. (Safed Kaunch)
In this study, a nonrhizobial endophytic bacterial strain was isolated from the root nodules of the medicinal legume Mucuna utilis var. capitata L.; phenotypic, genotypic, and agricultural characterization was performed using a HiMedia kit and 16S rRNA sequencing. This strain showed tremendous seedling growth potential (30%), compared with the control, as well as a strong antagonistic nature against the plant pathogenic fungus Fusarium udum when plant growth parameters were analyzed. The strain, identified by 16S rRNA as Stenotrophomonas maltophilia, showed a multitude of plant-growth-promoting attributes both direct (IAA,...
Source: Canadian Journal of Microbiology - July 7, 2020 Category: Microbiology Authors: Abhinav Aeron Ramesh Chandra Dubey Dinesh Kumar Maheshwari Source Type: research

Potential impact of SARS-CoV-2 infection on the thymus
Canadian Journal of Microbiology, Ahead of Print. Understanding the pathogenesis of certain viral agents is essential for developing new treatments and obtaining a clinical cure. With the onset of the new coronavirus (SARS-CoV-2) pandemic in the beginning of 2020, a rush to conduct studies and develop drugs has led to the publication of articles that seek to address knowledge gaps and contribute to the global scientific research community. There are still no reports on the infectivity or repercussions of SARS-CoV-2 infection on the central lymphoid organ, the thymus, nor on thymocytes or thymic epithelial cells. In this br...
Source: Canadian Journal of Microbiology - July 7, 2020 Category: Microbiology Authors: Marvin Paulo Lins Salete Smaniotto Source Type: research

Potential impact of SARS-CoV-2 infection on the thymus
Canadian Journal of Microbiology, e-First Articles. Understanding the pathogenesis of certain viral agents is essential for developing new treatments and obtaining a clinical cure. With the onset of the new coronavirus (SARS-CoV-2) pandemic in the beginning of 2020, a rush to conduct studies and develop drugs has led to the publication of articles that seek to address knowledge gaps and contribute to the global scientific research community. There are still no reports on the infectivity or repercussions of SARS-CoV-2 infection on the central lymphoid organ, the thymus, nor on thymocytes or thymic epithelial cells. In this ...
Source: Canadian Journal of Microbiology - July 7, 2020 Category: Microbiology Authors: Marvin Paulo Lins Salete Smaniotto Source Type: research

Characterization of a plant-growth-promoting non-nodulating endophytic bacterium (Stenotrophomonas maltophilia) from the root nodules of Mucuna utilis var. capitata L. (Safed Kaunch)
In this study, a nonrhizobial endophytic bacterial strain was isolated from the root nodules of the medicinal legume Mucuna utilis var. capitata L.; phenotypic, genotypic, and agricultural characterization was performed using a HiMedia kit and 16S rRNA sequencing. This strain showed tremendous seedling growth potential (30%), compared with the control, as well as a strong antagonistic nature against the plant pathogenic fungus Fusarium udum when plant growth parameters were analyzed. The strain, identified by 16S rRNA as Stenotrophomonas maltophilia, showed a multitude of plant-growth-promoting attributes both direct (IAA,...
Source: Canadian Journal of Microbiology - July 7, 2020 Category: Microbiology Authors: Abhinav Aeron Ramesh Chandra Dubey Dinesh Kumar Maheshwari Source Type: research

Screening of thermosensitive autolytic mutant brewer ’s yeast and transcriptomic analysis of heat stress response
In this study, a thermosensitive autolytic brewer’s yeast P-510 was obtained with atmospheric and room temperature plasma mutagenesis plus 5-bromo-chloro-3-indolyl phosphate screening. The mutant rapidly autolyzed at 37 °C and the autolysates contained more active components and showed higher antioxidant activities compared with that of the parental strain, which i ndicated that the mutant’s autolysates can potentially be used as functional food and nutritional ingredients. Transcriptomic analysis of the mutant and parental strains at 28 and 37 °C suggested that thermosensitive autolysis of P-510 was probably caused ...
Source: Canadian Journal of Microbiology - July 2, 2020 Category: Microbiology Authors: Mingfang Zhang Jinjing Wang Chengtuo Niu Feiyun Zheng Chunfeng Liu Qi Li Source Type: research

Screening of thermosensitive autolytic mutant brewer ’s yeast and transcriptomic analysis of heat stress response
In this study, a thermosensitive autolytic brewer’s yeast P-510 was obtained with atmospheric and room temperature plasma mutagenesis plus 5-bromo-chloro-3-indolyl phosphate screening. The mutant rapidly autolyzed at 37 °C and the autolysates contained more active components and showed higher antioxidant activities compared with that of the parental strain, which i ndicated that the mutant’s autolysates can potentially be used as functional food and nutritional ingredients. Transcriptomic analysis of the mutant and parental strains at 28 and 37 °C suggested that thermosensitive autolysis of P-510 was probably caused ...
Source: Canadian Journal of Microbiology - July 2, 2020 Category: Microbiology Authors: Mingfang Zhang Jinjing Wang Chengtuo Niu Feiyun Zheng Chunfeng Liu Qi Li Source Type: research

Bioprospection of native psychrotolerant plant-growth-promoting rhizobacteria from Peruvian Andean Plateau soils associated with Chenopodium quinoa
Canadian Journal of Microbiology, Ahead of Print. The Peruvian Andean Plateau, one of the main production areas of native varieties of Chenopodium quinoa, is exposed to abrupt decreases in environmental temperature, affecting crop production. Plant-growth-promoting rhizobacteria that tolerate low temperatures could be used as organic biofertilizers in this region. We aimed to bioprospect the native psychrotolerant bacteria of the quinoa rhizosphere in this region that show plant-growth-promoting traits. Fifty-one strains belonging to the quinoa rhizosphere were characterised; 73% of the total could grow at low temperatures...
Source: Canadian Journal of Microbiology - June 22, 2020 Category: Microbiology Authors: Carolina Chumpitaz-Segovia D ébora Alvarado Katty Ogata-Guti érrez Doris Z úñiga-Dávila Source Type: research

Bioprospection of native psychrotolerant plant-growth-promoting rhizobacteria from Peruvian Andean Plateau soils associated with Chenopodium quinoa
Canadian Journal of Microbiology, e-First Articles. The Peruvian Andean Plateau, one of the main production areas of native varieties of Chenopodium quinoa, is exposed to abrupt decreases in environmental temperature, affecting crop production. Plant-growth-promoting rhizobacteria that tolerate low temperatures could be used as organic biofertilizers in this region. We aimed to bioprospect the native psychrotolerant bacteria of the quinoa rhizosphere in this region that show plant-growth-promoting traits. Fifty-one strains belonging to the quinoa rhizosphere were characterised; 73% of the total could grow at low temperatur...
Source: Canadian Journal of Microbiology - June 22, 2020 Category: Microbiology Authors: Carolina Chumpitaz-Segovia D ébora Alvarado Katty Ogata-Guti érrez Doris Z úñiga-Dávila Source Type: research

A short overview of the CRISPR-Cas adaptation stage
Canadian Journal of Microbiology, Ahead of Print. CRISPR research began over 30 years ago with the incidental discovery of an unusual nucleotide arrangement in the Escherichia coli genome. It took 20 years to find the main function of CRISPR-Cas systems as an adaptive defence mechanism against invading nucleic acids, and our knowledge of their biology has steadily increased ever since. In parallel, the number of applications derived from CRISPR-Cas systems has risen spectacularly. The CRISPR-based genome editing tool is arguably the most exciting application in both basic and applied research. Lately, CRISPR-Cas research h...
Source: Canadian Journal of Microbiology - June 18, 2020 Category: Microbiology Authors: Cas Mosterd Genevi ève M. Rousseau Sylvain Moineau Source Type: research

A short overview of the CRISPR-Cas adaptation stage
Canadian Journal of Microbiology, e-First Articles. CRISPR research began over 30 years ago with the incidental discovery of an unusual nucleotide arrangement in the Escherichia coli genome. It took 20 years to find the main function of CRISPR-Cas systems as an adaptive defence mechanism against invading nucleic acids, and our knowledge of their biology has steadily increased ever since. In parallel, the number of applications derived from CRISPR-Cas systems has risen spectacularly. The CRISPR-based genome editing tool is arguably the most exciting application in both basic and applied research. Lately, CRISPR-Cas research...
Source: Canadian Journal of Microbiology - June 18, 2020 Category: Microbiology Authors: Cas Mosterd Genevi ève M. Rousseau Sylvain Moineau Source Type: research