Note of appreciation
Canadian Journal of Microbiology,Volume 67, Issue 1, Page iii-iv, January 2021. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - January 4, 2021 Category: Microbiology Source Type: research
In vitro degradation of low-density polyethylene by new bacteria from larvae of the greater wax moth, Galleria mellonella
Canadian Journal of Microbiology, Ahead of Print. Three bacterial species isolated from whole body extracts of the greater wax moth larvae, Galleria mellonella, were evaluated for their ability to utilize low-density polyethylene (LDPE) as a sole carbon source in vitro. These bacteria were identified as Lysinibacillus fusiformis, Bacillus aryabhattai, and Microbacterium oxydans. Their ability to biodegrade LDPE was assessed by growth curves, cell biomass production, polyethylene (PE) weight loss, and the presence of LDPE hydrolysis products in the growth m edia. Consortia of these bacteria with three other bacteria previ...
Source: Canadian Journal of Microbiology - December 11, 2020 Category: Microbiology Authors: Zahra Montazer Mohammad B. Habibi Najafi David B. Levin Source Type: research
Do methanotrophs drive phosphorus mineralization in soil ecosystem?
Canadian Journal of Microbiology, Ahead of Print. Experiments were carried out to elucidate linkage between methane consumption and mineralization of phosphorous (P) from different P sources. The treatments were (i) no CH4 + no P amendment (absolute control), (ii) with CH4 + no P amendment (control), (iii) with CH4 + inorganic P as Ca3(PO4)2, and (iv) with CH4 + organic P as sodium phytate. P sources were added at 25 µg P·(g soil)−1. Soils were incubated to undergo three repeated CH4 feeding cycles , referred to as feeding cycle I, feeding cycle II, and feeding cycle III. CH4 consumption rate k (µg CH4 con...
Source: Canadian Journal of Microbiology - December 10, 2020 Category: Microbiology Authors: Santosh Ranjan Mohanty Adarsh Kumar Rakesh Parmar Garima Dubey Ashok Patra Bharati Kollah Source Type: research
Frontrunners in the race to develop a SARS-CoV-2 vaccine
Canadian Journal of Microbiology, Ahead of Print. Numerous studies continue to be published on the COVID-19 pandemic that is being caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Given the rapidly evolving global response to SARS-CoV-2, here we primarily review the leading COVID-19 vaccine strategies that are currently in Phase III clinical trials. Nonreplicating viral vector strategies, inactivated virus, recombinant protein subunit vaccines, and nucleic acid vaccine platforms are all being pursued in an effort to combat the infection. Preclinical and clinal trial results of these efforts are...
Source: Canadian Journal of Microbiology - December 2, 2020 Category: Microbiology Authors: Raquel L. Russell Peter Pelka Brian L. Mark Source Type: research
Occurrence of extended-spectrum β-lactamase and AmpC-producing Escherichia coli in retail meat products from the Maritime Provinces, Canada
This study was conducted to determine the occurrence of antimicrobial resistance to the extended-spectrum cephalosporins (ESC) in Escherichia coli isolates. The isolates were collected from retail meat products collected in the Maritime Provinces of Canada. Our analyses involved the use of both selective and traditional culture methods; we also conducted genotype analyses using multiplex polymerase chain reactions. ESC-resistant (ESC-R) E. coli were detected in 33 of 559 samples (5.9%) using the traditional culture method, compared with 151 of 557 samples (27.1%) using the selective culture method. We recovered more isolat...
Source: Canadian Journal of Microbiology - November 26, 2020 Category: Microbiology Authors: Babafela Awosile Jessica Eisnor Matthew E. Saab Luke Heider J T. McClure Source Type: research
Comparison of fecal microbiota composition of blue sheep fed Lolium perenne versus Sorghum sudanense
Canadian Journal of Microbiology, Ahead of Print. Lolium perenne L. and Sorghum sudanense (Piper) Stapf. are 2 common forages fed to blue sheep (Pseudois nayaur Hodgson, 1833) in captivity. However, the effect of these 2 forages on the gastrointestinal microbiota is largely unknown. We analyzed the diversity of the microbiota in the feces of captive blue sheep fed with L. perenne (group F1) and S. sudanense (group F2) by 16S rRNA sequencing. A total of 20 major phyla and 29 genera fecal bacterial communities were detected in the 2 groups. The F1 and F2 groups shared common microbiota at the phylum level, which mainly c...
Source: Canadian Journal of Microbiology - November 26, 2020 Category: Microbiology Authors: Haili Wu Yaohua Yuan Hongjie Pan Source Type: research
Strain improvement of Trichoderma spp. through two-step protoplast fusion for cellulase production enhancement
In conclusion, the results demonstrate that a 3-interspecific hybrid strain was isolated, with improved characteristics for cellulase degradation and showing genetic polymorphisms and d ifferences in the volatile profile, suggesting reorganizations at the genetic level. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - November 23, 2020 Category: Microbiology Authors: Zahra Papzan Mojegan Kowsari Mohammad Javan-Nikkhah Amir Mirzadi Gohari M. Carmen Lim ón Source Type: research
Endophytic bacterial communities and spatiotemporal variations in cotton roots in Xinjiang, China
Canadian Journal of Microbiology, Ahead of Print. Endogenous bacteria are important for maintaining the health and other ecologically relevant functions of cotton plants. However, little is known about the community structures and diversity of endophytic bacteria in cotton plants. In our study, we used the Illumina amplicon sequencing technology to study the endophytic bacteria found in cotton root tissue in Xinjiang, China. A total of 60.84 × 106 effective sequences of the 16S rRNA gene in the V5–V6 variable region revealed a large number of operational taxonomic units (OTUs), namely 81–338 OTUs, at a cut-off level o...
Source: Canadian Journal of Microbiology - November 12, 2020 Category: Microbiology Authors: YingWu Shi HongMei Yang Ming Chu XinXiang Niu XiangDong Huo Yan Gao Qing Lin Jun Zeng Tao Zhang Kai Lou Source Type: research
Homodimerization and heterodimerization requirements of Acinetobacter baumannii SOS response coregulators UmuDAb and DdrR revealed by two-hybrid analyses
Canadian Journal of Microbiology, Ahead of Print. The multidrug-resistant pathogen Acinetobacter baumannii displays unusual control of its SOS mutagenesis genes, as it does not encode a LexA repressor, but instead employs the UmuDAb repressor and a small protein, DdrR, that is uniquely found in Acinetobacter species. We used bacterial adenylate cyclase two-hybrid analyses to determine if UmuDAb and DdrR coregulation might involve physical interactions. Neither quantitative nor qualitative assays showed UmuDAb interaction with DdrR. DdrR hybrid proteins, however, demonstrated modest head-to-tail interactions in a qualitativ...
Source: Canadian Journal of Microbiology - November 12, 2020 Category: Microbiology Authors: Deborah Cook Jordan Carrington Kevin Johnson Janelle Hare Source Type: research
mfsQ encoding an MFS efflux pump mediates adaptive protection of Stenotrophomonas maltophilia against benzalkonium chloride
Canadian Journal of Microbiology, Ahead of Print. The persistence of Stenotrophomonas maltophilia, especially in hospital environments where disinfectants are used intensively, is one of the important factors that allow this opportunistic pathogen to establish nosocomial infections. In the present study, we illustrated that S. maltophilia possesses adaptive resistance to the disinfectant benzalkonium chloride (BAC). This BAC adaptation was abolished in the ΔmfsQ mutant, in which a gene encoding an efflux transporter belonging to the major facilitator superfamily (MFS) was deleted. The ΔmfsQ mutant also showed increase ...
Source: Canadian Journal of Microbiology - November 5, 2020 Category: Microbiology Authors: Jurairat Chittrakanwong Nisanart Charoenlap Skorn Mongkolsuk Paiboon Vattanaviboon Source Type: research
Growth effects in oregano plants (Origanum vulgare L.) assessment through inoculation of bacteria isolated from crop fields located on desert soils
In conclusion, we were able to determine that the bacterial interactions are not always positive or synergistic, but they also might be neutral or antagonistic. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - November 2, 2020 Category: Microbiology Authors: Abraham Loera-Muro Mar ía Goretty Caamal-Chan Thelma Castellanos Ang élica Luna-Camargo Trinidad Aguilar-D íaz Aar ón Barraza Source Type: research
A green sulfur bacterium from epsomitic Hot Lake, Washington, USA
Canadian Journal of Microbiology, Ahead of Print. Hot Lake is a small heliothermal and hypersaline lake in far north –central Washington State (USA) and is limnologically unusual because MgSO4 rather than NaCl is the dominant salt. In late summer, the Hot Lake metalimnion becomes distinctly green from blooms of planktonic phototrophs. In a study undertaken over 60 years ago, these blooms were predicted to inclu de green sulfur bacteria, but no cultures were obtained. We sampled Hot Lake and established enrichment cultures for phototrophic sulfur bacteria in MgSO4-rich sulfidic media. Most enrichments turned green or red...
Source: Canadian Journal of Microbiology - November 2, 2020 Category: Microbiology Authors: Michael T. Madigan Megan L. Kempher Kelly S. Bender Deborah O. Jung W. Matthew Sattley Stephen R. Lindemann Allan E. Konopka Alice C. Dohnalkova James K. Fredrickson Source Type: research
Staphylococcus aureus grown in anaerobic conditions exhibits elevated glutamine biosynthesis and biofilm units
Canadian Journal of Microbiology, Ahead of Print. The enormous spread of Staphylococcus aureus infections through biofilms is a major concern in hospital-acquired infections. Biofilm formation by S. aureus on any surface is facilitated by adjusting its redox status. This organism is a facultative anaerobe shift more towards reductive conditions by enhancing nitrogen metabolism where glutamine synthesis plays a key role. Glutamine is synthesized by glutamine synthetase (GS) encoded by the glnA gene. The gene was amplified by PCR from the chromosomal DNA of S. aureus, sequenced (HQ329146.1), and cloned. The pure recombinant ...
Source: Canadian Journal of Microbiology - November 2, 2020 Category: Microbiology Authors: Yugandhar Vudhya Gowrisankar Sunitha Manne Mudhu Santhosh Kumar Pasupuleti Subbarayudu Suthi Abhijit Chaudhury Potukuchi Venkata Gurunadha Krishna Sarma Source Type: research
COVID-19 basics and vaccine development with a Canadian perspective
Canadian Journal of Microbiology,Volume 67, Issue 2, Page 112-118, February 2021. The ongoing coronavirus disease 2019 (COVID-19) pandemic is a rapidly evolving situation. New discoveries about COVID-19 and its causative virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continue to deepen the understanding of this novel disease. As there is currently no COVID-19 specific treatment, isolation is the most effective method to prevent transmission. Moreover, development of a safe and effective COVID-19 vaccine will be instrumental in reinstating pre-COVID-19 conditions. As of 31 July 2020, there are at le...
Source: Canadian Journal of Microbiology - November 2, 2020 Category: Microbiology Authors: Marina Liu Xiongbiao Chen Source Type: research
The progress of type II persisters of Escherichia coli O157:H7 to a non-culturable state during prolonged exposure to antibiotic stress with revival being aided through acid-shock treatment and provision of methyl pyruvate
Canadian Journal of Microbiology, Ahead of Print. Persisters are a form of dormancy in bacteria that provide temporary resistance to antibiotics. The following reports on the formation of Escherichia coli O157:H7 E318 type II persisters from a protracted (8 days) challenge with ampicillin. Escherichia coli O157:H7 followed a multiphasic die-off pattern with an initial rapid decline (Phase I) of susceptible cells that transitioned to a slower rate representing tolerant cells (Phase II). After 24 h post-antibiotic challenge, the E. coli O157:H7 leve ls remained relatively constant at 2 log CFU/mL (Phase III), but became...
Source: Canadian Journal of Microbiology - October 30, 2020 Category: Microbiology Authors: Heather Chen Andrew Green Kailey Martz Xueyang Wu Abdulhakeem Alzahrani Keith Warriner Source Type: research
