Inhibitory relationships of resident bacteria isolated from the mantle fluids of Crassostrea virginica
Canadian Journal of Microbiology, Ahead of Print. Thirteen aerobic, halotolerant marine bacterial strains were isolated from the mantle fluids and associated mucus of the eastern oyster Crassostrea virginica harvested from the highly impacted Black Rock Harbor in western Long Island Sound. All isolated strains were Gram negative and had previously been identified using 16S RNA gene sequence analysis. These 13 strains were examined for their ability to inhibit the growth of each other employing a diffusion agar method used by antibiotic assays. All challenger strains were able to inhibit at least one of the indicator isolat...
Source: Canadian Journal of Microbiology - June 27, 2022 Category: Microbiology Authors: Phyllis C. Braun Ryan D. Bongo Source Type: research
The compact mitogenome of Ceratocystiopsis pallidobrunnea
Canadian Journal of Microbiology, Ahead of Print. Ceratocystiopsis is a fungal genus that has been assigned to the Ophiostomatales, fungi known for their association with various bark beetles and other arthropods. The mitochondrial genome of Ceratocystiopsis pallidobrunnea has been characterized and compared with other members of the genus Ceratocystiopsis and Ophiostomatales. At 29 022 bp, the mitogenome of C. pallidobrunnea is the smallest reported so far for this genus. Gene arrangement was observed to be conserved for this group of fungi, and mitogenome variation appears to be mostly due to the absence and presen...
Source: Canadian Journal of Microbiology - June 8, 2022 Category: Microbiology Authors: Alvan Wai Georg Hausner Source Type: research
Overexpression of the global regulator FnVeA upregulates antitumor substances in endophytic Fusarium nematophilum
Canadian Journal of Microbiology, Ahead of Print. The special niche of endophytic fungi promotes their potential to produce antitumor compounds with novel structure and significant bioactivity for screening of new antitumor drugs. In our previous studies, we isolated a Fusarium strain from the roots of the medicinal plant Nothapodytes pittosporoides and identified it as Fusarium nematophilum. We found that the crude extract of F. nematophilum had significant antitumor activity on A549 cancer cells, and overexpressing the global regulatory factor FnVeA (the VeA gene of the fungus F. nematophilum) resulted in a significant i...
Source: Canadian Journal of Microbiology - June 1, 2022 Category: Microbiology Authors: Min Qin Yongjie Li Lu Cai Xuemin Yin Zhangjiang He Jichuan Kang Source Type: research
Chitin degradation and its effect on natural transformation: a systematic genetic study in Vibrio parahaemolyticus
Canadian Journal of Microbiology, Ahead of Print. The degradation of polymeric chitin by chitinase liberates soluble N-acetyl glucosamine oligosaccharides (GlcNAcn ≥2), a source of nutrition that can also induce a state of natural genetic competence in Vibrio parahaemolyticus. This analysis revealed that among seven predicted chitinases, the synergistic action of VPA0055 (ChiA2), VP0619 (ChiB), and VPA0832 (Cdx) were essential for the robust growth and high transformation frequency on chitin. The endochitinase, ChiA2, and periplasmic chitinase, Cdx were indispensable for chitin degradation. ChiB was not essential for gro...
Source: Canadian Journal of Microbiology - May 27, 2022 Category: Microbiology Authors: Anusuya Debnath Shin-Ichi Miyoshi Source Type: research
The mitogenome of Urnula craterium
In this study, we investigated the mitochondrial genome of U. craterium. The biology and taxonomy of this fungus is poorly studied and there are no mitogenomes currently available for any member of the Sarcosomataceae (Order Pezizales). The complete mitogenome of U. craterium comprises 43 967 bps and encodes 14 protein-coding genes, a complete set of tRNAs and rRNA genes. A novel feature of the mitogenome is the presence of a single subunit DNA polymerase-coding region that is typically associated with linear invertron-type plasmids. The mitogenome may offer insights into the evo lution of mitogenomes among members of ...
Source: Canadian Journal of Microbiology - May 27, 2022 Category: Microbiology Authors: Jigeesha Mukhopadhyay Alvan Wai Leonard J. Hutchison Georg Hausner Source Type: research
Sequential markerless genetic manipulations of species from the Neisseria genus
Canadian Journal of Microbiology, Ahead of Print. The development of simple and highly efficient strategies for genetic modifications is essential for postgenetic studies aimed at characterizing gene functions for various applications. We sought to develop a reliable system for Neisseria species that allows for both unmarked and accumulation of multiple genetic modifications in a single strain. In this work, we developed and validated three-gene cassettes named RPLK and RPCC, comprising of an antibiotic resistance marker for positive selection, the phenotypic selection marker lacZ or mCherry, and the counterselection gene ...
Source: Canadian Journal of Microbiology - May 5, 2022 Category: Microbiology Authors: Sammy Nyongesa Martin Chenal Ève Bernet Florian Coudray Fr édéric J. Veyrier Source Type: research
A metagenomic study of the bacteria in snow algae microbiomes
Canadian Journal of Microbiology, Ahead of Print. The bacterial communities found in snow algae blooms have been described in terms of their 16S rRNA gene community profiles, but little information exists on their metabolic potential. Previously, we reported that several bacterial taxa are common across snow algae blooms in the southwestern mountains of the Coast Range in British Columbia, Canada. Here, we further this work by reporting a partial bacterial metagenome from the same snow algal microbiomes. Using shotgun metagenomic data, we constructed metagenomically assembled bacterial genomes (MAGs). Of the total 54 binne...
Source: Canadian Journal of Microbiology - May 5, 2022 Category: Microbiology Authors: Kurt M. Yakimovich Lynne M. Quarmby Source Type: research
Variations in microbial community on different materials in Sanya Marine Environment Experimental Station, China
This study provides basic data for marine material research, marine corrosion, and national defence construction. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - April 12, 2022 Category: Microbiology Authors: Shide Ma Yuhang Li Fang Guan Linlin Zhang Ju Li Yu Tai Haitao Ren Jizhou Duan Source Type: research
Recombinase polymerase amplification in the molecular diagnosis of microbiological targets and its applications
In this study, we present a review of the use of RPA in clinical settings and its implementation in various research areas. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - April 8, 2022 Category: Microbiology Authors: D.S. Mota J.M. Guimar ães A.M.D. Gandarilla J.C.B.S. Filho W.R. Brito L.A.M. Mari úba Source Type: research
Choice of cover crop influences soil fungal and bacterial communities in Prince Edward Island, Canada
In this study, eight cover crops, three mixed crops, and an unmanaged fallow control were evaluated over 2 years for their effects on the soil microbiome. Internal transcribed spacer (ITS) and 16S rRNA amplicon sequencing was performed to characterize fungal and bacterial communities in the soil during the cover crop growing season, and in the subsequent year. Fungal and bacterial alpha diversity significantly increased over time and were influenced in the subsequent growing season by choice of cover crops. Some fungal and bacterial trophic and functional groups were also affected by crop choice. Fungal pathotroph abundanc...
Source: Canadian Journal of Microbiology - March 28, 2022 Category: Microbiology Authors: Harini Aiyer Bourlaye Fofana Tandra Fraser Claude Caldwell Andrew McKenzie-Gopsill Aaron Mills Adam Foster Source Type: research
Effect of rapeseed straw-derived biochar on soil bacterial community structure at tillering stage of Oryza Sativa
Canadian Journal of Microbiology, Ahead of Print. Numerous studies have reported the dynamics of microbes when biochar was applied, whereas the information on the alterations of bacterial community after application of rapeseed straw-derived biochar is limited. A pot experiment with two rapeseed straw-derived biochar application treatments (with biochar application at the rate of 200 g/pot, C1, and without biochar application, 0 g/pot, C0) was conducted. No significant differences were observed in the number of operational taxonomic units, observed species, Shannon index, Simpson index, Chao1, ACE, and phylogenetic dive...
Source: Canadian Journal of Microbiology - March 28, 2022 Category: Microbiology Authors: Chunrong Zhao Xiaohong Yin Jiana Chen Fangbo Cao Salah F. Abou-Elwafa Min Huang Source Type: research
Antifungal activity of [K3]temporin-SHa against medically relevant yeasts and moulds
Canadian Journal of Microbiology, Ahead of Print. Few antifungal agents are currently available for the treatment of fungal infections. Antimicrobial peptides (AMPs), which are natural molecules involved in the innate immune response of many organisms, represent a promising research method because of their broad killing activity. The aim of this study was to assess the activity of a frog AMP, [K3]temporin-SHa, against some species of yeasts and moulds, and to further explore its activity against Candida albicans. MIC determinations were performed according to EUCAST guidelines. Next, the activity of [K3]temporin-SHa agains...
Source: Canadian Journal of Microbiology - March 14, 2022 Category: Microbiology Authors: K évin Brunet Julien Verdon Ali Ladram Simon Arnault Marie-H élène Rodier Estelle Cateau Source Type: research
Abundance of integrons in halophilic bacteria
Canadian Journal of Microbiology, Ahead of Print. Integrons are genetic platforms used for expressing open reading frames (ORFs) arranged in gene cassettes. Excision and integration of gene cassettes are controlled by their associated integron integrase (IntI). Using IntegronFinder software, we analyzed all complete halophilic genomes available in the HaloDom database, along with selected partial halophilic genomes. We identified 18 new complete bacterial integrons and 46 clusters of attC sites lacking a neighboring integron integrase (CALINs). Different classes of insertion sequences (ISs) were also identified within and ...
Source: Canadian Journal of Microbiology - March 3, 2022 Category: Microbiology Authors: Sarah Sonbol Rania Siam Source Type: research
In situ biochemical characterization of Venus cloud particles using a life-signature detection microscope
We report that pH adjustment using alkali was effective for obtaining fluorescence images and that fluorescence attenuation was observed after the adjustment, even when the acidophile suspension in concent rated sulfuric acid was used as a sample. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - March 2, 2022 Category: Microbiology Authors: Satoshi Sasaki Akihiko Yamagishi Yoshitaka Yoshimura Keigo Enya Atsuo Miyakawa Sosuke Ohno Kazuhisa Fujita Tomohiro Usui Sanjay S. Limaye Source Type: research
Proceedings of the Dual Use Research of Concern Panel Discussion: challenges and perspectives
Discussion, organized by Synbio Canada and the Alberta RNA Research and Training Institute (ARRTI), took place on June 23rd, 2021. It brought together six stakeholders from different levels of academic research, administration, governance, and science publishing to explore the current and future challenges in addressing DURC. Technological advancements within the life sciences, especially within the field of omics technology, make it difficult to apply a simple checklist for dual-use assessment and require continuous and integrated effort. Bottom-up approaches from within the scientific community are suggested by all stake...
Source: Canadian Journal of Microbiology - March 2, 2022 Category: Microbiology Authors: Fabian Rohden Christopher J. Nelson Christopher K. Yost Cody Anderson Rebecca Moritz Svenja Vinke Hans-Joachim Wieden Source Type: research
