Investigating the spatiotemporal dynamics of apple tree phyllosphere bacterial and fungal communities across cultivars in orchards
In this study, we investigated the determinants of the spatiotemporal variation in bacterial and fungal communities within the apple tree phyllosphere, employing 16S and ITS amplicon sequencing. Our research assessed the impact of key factors —plant compartment, site, time, and cultivar—on the composition and diversity of leaf and flower microbial communities. Our analyses, based on samples collected from three cultivars in three orchards in 2022, revealed that site and time are the strongest drivers of apple tree phyllosphere microb ial communities. Conversely, plant compartment and cultivar exhibited minor roles in e...
Source: Canadian Journal of Microbiology - April 18, 2024 Category: Microbiology Authors: Sophie Boutin Ema Lussier Isabelle Laforest-Lapointe Source Type: research
Spatial arrangement and density variations in the cell envelope of Deinococcus radiodurans
In this study, we delve into these aspects by integrating the state of the art with structural calculations. This approach provides crucial evidence supporting an evolutive pressure intricately linked to surface phenomena depending on the environmental conditions. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - March 25, 2024 Category: Microbiology Authors: Domenica Farci Dario Piano Source Type: research
Co-inoculation with novel nodule-inhabiting bacteria reduces the benefits of legume –rhizobium symbiosis
This study demonstrates that variation of NREs present in natural populations must be considered to better understand legume –rhizobium dynamics in soil communities. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - March 20, 2024 Category: Microbiology Authors: James C. Kosmopoulos Rebecca T. Batstone-Doyle Katy D. Heath Source Type: research
Biological and genomic characteristics of three novel bacteriophages and a phage-plasmid of Klebsiella pneumoniae
Canadian Journal of Microbiology, Ahead of Print. Bacteriophages have emerged as promising candidates for the treatment of difficult-to-treat bacterial infections. The aim of this study is to isolate and characterize phages infecting carbapenem-resistant and extended-spectrum beta-lactamase producer Klebsiella pneumoniae isolates. Water samples were taken for the isolation of bacteriophages. One-step growth curve, the optimal multiplicity of infection (MOI), thermal and pH stabilities, transmission electron microscopy and whole-genome sequencing of phages were studied. Four phages were isolated and named Klebsiella phage K...
Source: Canadian Journal of Microbiology - March 6, 2024 Category: Microbiology Authors: Aylin Uskudar-Guclu Sezin Unlu Hanife Salih-Dogan Suleyman Yalcin Ahmet Basustaoglu Source Type: research
Plant seedlings of peas, tomatoes, and cucumbers exude compounds that are needed for growth and chemoattraction of Rhizobium leguminosarum bv. viciae 3841 and Azospirillum brasilense Sp7
This study characterizes seedling exudates of peas, tomatoes, and cucumbers at the level of chemical composition and functionality. A plant experiment confirmed that Rhizobium leguminosarum bv. viciae 3841 enhanced growth of pea shoots, while Azospirillum brasilense Sp7 supported growth of pea, tomato, and cucumber roots. Chemical analysis of exudates after 1 day of seedling incubation in water yielded differences between the exudates of the three plants. Most remarkably, cucumber seedling exudate did not contain detectable sugars. All exudates contained amino acids, nucleobases/nucleosides, and organic acids, among other ...
Source: Canadian Journal of Microbiology - March 1, 2024 Category: Microbiology Authors: Fatema A. Nisha Janice N.A. Tagoe Amanda B. Pease Shelley M. Horne Angel Ugrinov Barney A. Geddes Birgit M. Pr üß Source Type: research
High diversity within and low but significant genetic differentiation among geographic and temporal populations of the global Streptococcus pneumoniae
In this study, we investigated the spatial and temporal patterns of genetic variation of S. pneumoniae based on archived multilocus sequence typing data from PubMLST.org. Our analyses demonstrated both shared and unique distributions of sequence types (STs) and allele types among regional populations. Among the 17 915 global STs, 36 representing 15 263 isolates were broadly shared among all six continents, consistent with recent clonal dispersal and expansion of this pathogen. The analysis of molecular variance revealed that >96% genetic variations were found within individual regional populations. However, though...
Source: Canadian Journal of Microbiology - February 29, 2024 Category: Microbiology Authors: Jezreel Dalmieda Megan Hitchcock Jianping Xu Source Type: research
QsvR and OpaR coordinately regulate the transcription of cpsS and cpsR in Vibrio parahaemolyticus
In this study, the results of quantitative real-time PCR and LacZ fusion assays demonstrated that deletion of qsvR and/or opaR significantly decreased the expression levels of cpsS and cpsR compared to the wild-type strain. However, the results of two-plasmid lacZ reporter and electrophoretic mobili ty-shift assays showed that both QsvR and OpaR were unable to bind the regulatory DNA regions of cpsS and cpsR. Therefore, transcription of cpsS and cpsR was coordinately and indirectly activated by QsvR and OpaR. This work enriched our knowledge on the regulatory network of biofilm formation in V. parahaemolyticus. (Source: Ca...
Source: Canadian Journal of Microbiology - February 28, 2024 Category: Microbiology Authors: Xue Li Wei Lian Miaomiao Zhang Xi Luo Yiquan Zhang Renfei Lu Source Type: research
Do we still need Illumina sequencing data? Evaluating Oxford Nanopore Technologies R10.4.1 flow cells and the Rapid v14 library prep kit for Gram negative bacteria whole genome assemblies
Canadian Journal of Microbiology, Ahead of Print. The best whole genome assemblies are currently built from a combination of highly accurate short-read sequencing data and long-read sequencing data that can bridge repetitive and problematic regions. Oxford Nanopore Technologies (ONT) produce long-read sequencing platforms and they are continually improving their technology to obtain higher quality read data that is approaching the quality obtained from short-read platforms such as Illumina. As these innovations continue, we evaluated how much ONT read coverage produced by the Rapid Barcoding Kit v14 (SQK-RBK114) is necessa...
Source: Canadian Journal of Microbiology - February 14, 2024 Category: Microbiology Authors: Nicole Lerminiaux Ken Fakharuddin Michael R. Mulvey Laura Mataseje Source Type: research
Characterization of hydrocarbon degraders from Northwest Passage beach sediments and assessment of their ability for bioremediation
In this study, the characterization and genomic analyses of 22 hydrocarbon-biodegradative bacterial isolates revealed that they contained a diverse range of key alkane and aromatic hydrocarbon-degradative genes, as well as cold and salt tolerance genes indicating they are highly adapted to the extreme Arctic environment. Some isolates successfully degraded Ultra Low Sulfur Fuel Oil (ULSFO) at temperatures as low as −5 °C and high salinities (3%–10%). Three isolates were grown in liquid medium containing ULSFO as sole carbon source over 3 months and variation of hydrocarbon concentration was measured at three time poi...
Source: Canadian Journal of Microbiology - February 13, 2024 Category: Microbiology Authors: Antoine-O. Lirette Ya-Jou Chen Nastasia J. Freyria Esteban G óngora Charles W. Greer Lyle G. Whyte Source Type: research
Genome and transcriptome sequencing of Trichoderma harzianum T4, an important biocontrol fungus of Rhizoctonia solani, reveals genes related to mycoparasitism
Canadian Journal of Microbiology, Ahead of Print. Trichoderma harzianum is a well-known biological control strain and a mycoparasite of Rhizoctonia solani. To explore the mechanisms of mycoparasitism, the genome and transcriptome of T. harzianum T4 were both assembled and analyzed in this study. The genome of T. harzianum T4 was assembled into 106 scaffolds, sized 41.25 Mb, and annotated with a total of 8118 predicted genes. We analyzed the transcriptome of T. harzianum T4 against R. solani in a dual culture in three culture periods: before contact (BC), during contact (C), and after contact (AC). Transcriptome sequencing ...
Source: Canadian Journal of Microbiology - February 5, 2024 Category: Microbiology Authors: Yaping Wang Jian Wang Xiaochong Zhu Wei Wang Source Type: research
Unveiling the fungal color palette: pigment analysis of Fusarium solani species complex and Curvularia verruculosa clinical isolates
Canadian Journal of Microbiology, Ahead of Print. Fungal species in the Nectriaceae, such as Fusarium spp. (Hypocreales: Nectriaceae), are etiologic agents of hyalohyphomycosis capable of producing violaceous or yellowish pigments under certain conditions, while Curvularia spp. (Pleosporales: Pleosporaceae) are agents of phaeohyphomycosis and typically produce melanin in their cell walls. In nectriaceous and pleosporaceous fungi, these pigments are mainly constituted by polyketides (e.g., azaphilones, naphthoquinones, and hydroxyanthraquinones). Considering the importance of pigments synthesized by these genera, this work ...
Source: Canadian Journal of Microbiology - January 17, 2024 Category: Microbiology Authors: Luis Enrique Chaves-Gonz ález Daniela Jaikel-V íquez Stefany Lozada-Alvarado Fabio Granados-Chinchilla Source Type: research
Inhibition of the type IV secretion system from antibiotic-resistant Helicobacter pylori clinical isolates supports the potential of Cag α as an anti-virulence target
Canadian Journal of Microbiology, Ahead of Print. Helicobacter pylori resistance to antibiotics is a growing problem and it increasingly leads to treatment failure. While the bacterium is present worldwide, the severity of clinical outcomes is highly dependent on the geographical origin and genetic characteristics of the strains. One of the major virulence factors identified in H. pylori is the cag pathogenicity island (cagPAI), which encodes a type IV secretion system (T4SS) used to translocate effectors into human cells. Here, we investigated the genetic variability of the cagPAI among 13 antibiotic-resistant H. pylori s...
Source: Canadian Journal of Microbiology - January 4, 2024 Category: Microbiology Authors: Flore Oudouhou Claire Morin Mickael Bouin Christiane Gaudreau Christian Baron Source Type: research
Note of appreciation
Canadian Journal of Microbiology,Volume 70, Issue 1, Page i-i, January 2024. (Source: Canadian Journal of Microbiology)
Source: Canadian Journal of Microbiology - January 1, 2024 Category: Microbiology Source Type: research
Enterococcus faecium inhibits NF- κB/NLRP3/Caspase-1 signaling pathway to antagonize enterotoxigenic Escherichia coli-mediated inflammatory response
This study aimed to investigate the protective effect of Enterococcus faecium (E. faecium) against ETEC infection in mice through oral gavage. Morphological changes were examined through light microscopy. The expressions of inflammatory cytokines (IL-1 β, IL-6, TNF-α, IL-10, NF-κB, and NLRP3), tight junction protein (ZO-1, Claudin-1), and pyroptosis (Caspase-1, Caspase-4, and gasdermin D (GSDMD)) were detected using immunohistochemistry and quantitative real-time PCR. The results indicate that ETEC infection triggers the activation of inflammat ion-related pathways (NF-κB) and NLRP3 inflammasome, leading to the express...
Source: Canadian Journal of Microbiology - December 22, 2023 Category: Microbiology Authors: Huibin Zheng Sicheng Pu Jiahao Liu Falong Yang Dechun Chen Source Type: research
Hydrogen sulfide regulates arsenic-induced cell death in yeast cells by modulating the antioxidative system
In this study, the role of NaHS (exogenous physiological H2S) in alleviating As-induced yeast cell death was investigated. Yeast cells in the logarithmic phase were pretreated with 0.05 mmol/L NaHS for 6 h, and then incubated in the YPD medium with or without 1 mmol/L As. After 12 h of treatment, relative survival rate, H2S content, oxidative stress biomarkers, and antioxidant machinery were measured. Our results showed that sodium arsenite-induced yeast cell death and pretrea tment with 0.05 mmol/L NaHS significantly alleviated sodium arsenite-induced cell death. Under sodium arsenite conditions, the levels of intra...
Source: Canadian Journal of Microbiology - December 14, 2023 Category: Microbiology Authors: Lihua Wu Xia Yao Haiyan Li Yanfei Chen Source Type: research
