Development of a method for phycocyanin recovery from filamentous cyanobacteria and evaluation of its stability and antioxidant capacity
Most commercial phycocyanins are extracted from a filamentous cyanobacterium, Arthrospira (Spirulina) platensis. Owing to the expenses of culture and complexities of the physical and chemical methods of phycocyan... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 16, 2021 Category: Biotechnology Authors: Jinichi Aoki, Daisaku Sasaki and Munehiko Asayama Tags: Research Source Type: research

The engineered expression of secreted HSPB5-Fc in CHO cells exhibits cytoprotection in vitro
HSPB5 is an ATP-independent molecular chaperone that is induced by heat shock or other proteotoxic stresses. HSPB5 is cytoprotective against stress both intracellularly and extracellularly. It acts as a potent... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 14, 2021 Category: Biotechnology Authors: Jing Li, Jingjing Yu, Wenxian Xue, Huili Huang, Longjun Yan, Fan Sang, Shuangshuang An, Jing Zhang, Mingli Wang, Jun Zhang, Hui Li, Xiukun Cui, Jiang He and Yanzhong Hu Tags: Research Source Type: research

Insights from molecular docking and molecular dynamics on the potential of vitexin as an antagonist candidate against lipopolysaccharide (LPS) for microglial activation in neuroinflammation
Neuroinflammation has been identified to be the key player in most neurodegenerative diseases. If neuroinflammation is left to be unresolved, chronic neuroinflammation will be establish. Such situation is due ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 5, 2021 Category: Biotechnology Authors: M. A. F. Yahaya, A. R. Abu Bakar, J. Stanslas, N. Nordin, M. Zainol and M. Z. Mehat Tags: Research Source Type: research

Recombinant laccase rPOXA 1B real-time, accelerated and molecular dynamics stability study
Laccases (EC 1.10.3.2) are multi-copper oxidoreductases with great biotechnological importance due to their high oxidative potential and utility for removing synthetic dyes, oxidizing phenolic compounds, and d... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 4, 2021 Category: Biotechnology Authors: Leidy D. Ardila-Leal, Pedro A. Monterey-Guti érrez, Raúl A. Poutou-Piñales, Balkys E. Quevedo-Hidalgo, Johan F. Galindo and Aura M. Pedroza-Rodríguez Tags: Research Source Type: research

Designing, optimization, and validation of whole blood direct T-ARMS PCR for precise and rapid genotyping of complex vertebral malformation in cattle
DNA testing in the cattle industry undergoes multiple hurdles. Successful genotyping involves the transportation of samples from the field to the laboratory in a chilled environment followed by DNA extraction,... (Source: BMC Biotechnology)
Source: BMC Biotechnology - May 22, 2021 Category: Biotechnology Authors: R. R. Alyethodi, U. Singh, S. Kumar, R. Alex, G. S. Sengar, T. V. Raja, R. Deb and B. Prakash Tags: Methodology article Source Type: research

Comparative evaluation of different molecular methods for DNA extraction from individual Teladorsagia circumcincta nematodes
The purpose of this study was to develop a reliable DNA extraction protocol to use on individual Teladorsagia circumcincta nematode specimens to produce high quality DNA for genome sequencing and phylogenetic ana... (Source: BMC Biotechnology)
Source: BMC Biotechnology - May 17, 2021 Category: Biotechnology Authors: S. Sloan, C. J. Jenvey, D. Piedrafita, S. Preston and M. J. Stear Tags: Research article Source Type: research

Production of IgY polyclonal antibody against diphtheria toxin and evaluation of its neutralization effect by Vero cell assay
Diphtheria is a bacterial disease which is caused by Corynebacterium diphtheriae. The symptoms are due to the diphtheria toxin produced by the bacteria. Antibiotic therapy and the use of diphtheria antitoxin is a... (Source: BMC Biotechnology)
Source: BMC Biotechnology - May 12, 2021 Category: Biotechnology Authors: Morteza Rezaeifard, Roya Solhi, Mohammad Mohammadi, Ebrahim Abbasi and Mahdi Aminian Tags: Research article Source Type: research

Optimization and scale-up of α-amylase production by Aspergillus oryzae using solid-state fermentation of edible oil cakes
Amylases produced by fungi during solid-state fermentation are the most widely used commercial enzymes to meet the ever-increasing demands of the global enzyme market. The use of low-cost substrates to curtail... (Source: BMC Biotechnology)
Source: BMC Biotechnology - May 4, 2021 Category: Biotechnology Authors: M. Balakrishnan, G. Jeevarathinam, S. Kiran Santhosh Kumar, Iniyakumar Muniraj and Sivakumar Uthandi Tags: Research article Source Type: research

Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability
Ramie degumming is often carried out at high temperatures; therefore, thermostable alkaline pectate lyase (PL) is beneficial for ramie degumming for industrial applications. Thermostable PLs are usually obtain... (Source: BMC Biotechnology)
Source: BMC Biotechnology - May 3, 2021 Category: Biotechnology Authors: Zhanping Zhou and Xiao Wang Tags: Research Source Type: research

Evaluating the biocontrol potential of Canadian strain Bacillus velezensis 1B-23 via its surfactin production at various pHs and temperatures
Microorganisms, including Bacillus species are used to help control plant pathogens, thereby reducing reliance on synthetic pesticides in agriculture. Bacillus velezensis strain 1B-23 has been shown to reduce sym... (Source: BMC Biotechnology)
Source: BMC Biotechnology - April 29, 2021 Category: Biotechnology Authors: Michelle S. M. Li, David A. Piccoli, Tim McDowell, Jacqueline MacDonald, Justin Renaud and Ze-Chun Yuan Tags: Research Source Type: research

Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA
Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is t... (Source: BMC Biotechnology)
Source: BMC Biotechnology - April 23, 2021 Category: Biotechnology Authors: Zahra Heidari, Seyedeh Elham Rezatofighi and Saadat Rastegarzadeh Tags: Research article Source Type: research

Quantifying 35 transcripts in a single tube: model-based calibration of the GeXP multiplex RT-PCR assay
Quantitative analysis of differential gene expression is of central importance in molecular life sciences. The Gene eXpression Profiling technology (GeXP) relies on multiplex RT-PCR and subsequent capillary el... (Source: BMC Biotechnology)
Source: BMC Biotechnology - April 14, 2021 Category: Biotechnology Authors: Pauline Marquardt, Britta Werthmann, Viktoria R ätzel, Markus Haas and Wolfgang Marwan Tags: Methodology article Source Type: research

Cytotoxicity activities and chemical characteristics of exopolysaccharides and intracellular polysaccharides of Physarum polycephalum microplasmodia
Microbial polysaccharides have been reported to possess remarkable bioactivities. Physarum polycephalum is a species of slime mold for which the microplasmodia are capable of rapid growth and can produce a signif... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 27, 2021 Category: Biotechnology Authors: Tuyen T. H. Do, Tran N. B. Lai, Steven L. Stephenson and Hanh T. M. Tran Tags: Research article Source Type: research

Identification and characterization of a new soybean promoter induced by Phakopsora pachyrhizi, the causal agent of Asian soybean rust
Phakopsora pachyrhizi is a biotrophic fungal pathogen responsible for the Asian soybean rust disease causing important yield losses in tropical and subtropical soybean-producing countries. P. pachyrhizi triggers ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 25, 2021 Category: Biotechnology Authors: L. Cabre, S. Peyrard, C. Sirven, L. Gilles, B. Pelissier, S. Ducerf and N. Poussereau Tags: Research article Source Type: research

In vitro assessment of two novel Cellulases from Trabulsiella odontotermitis for agricultural waste utilization
The production of agricultural wastes still growing as a consequence of the population growing. However, the majority of these residues are under-utilized due their chemical composition, which is mainly compos... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 23, 2021 Category: Biotechnology Authors: Martha Mar ía Arevalos-Sánchez, Adrián Omar Maynez-Perez, Felipe A. Rodríguez-Almeida, José Alfredo Martínez-Quintana, Fidel Alejandro Sanchez-Flores, Monserrath Felix-Portillo, América Chavéz-Martínez, Myrna Elena Olvera-García, Oscar Ruiz-Barr Tags: Research article Source Type: research