Biobanked human foreskin epithelial cell sheets reduce inflammation and promote wound healing in a nude mouse model
Human epithelial cell sheets (ECSs) are used to clinically treat epithelial conditions such as burns, corneal blindness, middle ear cholesteatoma and vitiligo. As a widely used material in clinic, there is lit... (Source: BMC Biotechnology)
Source: BMC Biotechnology - February 2, 2021 Category: Biotechnology Authors: Dongliang Zhang, Jialiang Shao, Jingming Zhuang, Shukui Zhou, Shuo Yin, Fuyue Wu, Jiangang Hou and Xiang Wang Tags: Research article Source Type: research
CRISPR/Cas9-mediated knockout of clinically relevant alloantigenes in human primary T cells
The ability of CRISPR/Cas9 to mutate any desired genomic locus is being increasingly explored in the emerging area of cancer immunotherapy. In this respect, current efforts are mostly focused on the use of aut... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 29, 2021 Category: Biotechnology Authors: Elahe Kamali, Fatemeh Rahbarizadeh, Zohreh Hojati and Morten Fr ödin Tags: Research article Source Type: research
Comparing various protocols of human and bovine ovarian tissue decellularization to prepare extracellular matrix-alginate scaffold for better follicle development in vitro
Nowadays, the number of cancer survivors is significantly increasing as a result of efficient chemo/radio therapeutic treatments. Female cancer survivors may suffer from decreased fertility. In this regard, di... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 20, 2021 Category: Biotechnology Authors: Hossein Nikniaz, Zahra Zandieh, Mohammad Nouri, Neda Daei-farshbaf, Reza Aflatoonian, Mazaher Gholipourmalekabadi and Seyed Behnamedin Jameie Tags: Research article Source Type: research
Different genome-wide transcriptome responses of Nocardioides simplex VKM Ac-2033D to phytosterol and cortisone 21-acetate
Bacterial degradation/transformation of steroids is widely investigated to create biotechnologically relevant strains for industrial application. The strain of Nocardioides simplex VKM Ac-2033D is well known main... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 13, 2021 Category: Biotechnology Authors: Victoria Yu Shtratnikova, Mikhail I. S сhelkunov, Victoria V. Fokina, Eugeny Y. Bragin, Andrey A. Shutov and Marina V. Donova Tags: Research article Source Type: research
The osteogenic differentiation of human dental pulp stem cells in alginate-gelatin/Nano-hydroxyapatite microcapsules
Microcapsule is considered as a promising 3D microenvironment for Bone Tissue Engineering (BTE) applications. Microencapsulation of cells in an appropriate scaffold not only protected the cells against excess ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 11, 2021 Category: Biotechnology Authors: Mahdieh Alipour, Nima Firouzi, Zahra Aghazadeh, Mohammad Samiei, Soheila Montazersaheb, Ali Baradar Khoshfetrat and Marziyeh Aghazadeh Tags: Research article Source Type: research
Study on corrosion behavior of X80 steel under stripping coating by sulfate reducing bacteria
At present, microorganism has been considered as important factors that threaten to buried pipelines with disbonded coatings. Aiming at the problem of unknown corrosion mechanism of sulfate-reducing bacteria (... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 9, 2021 Category: Biotechnology Authors: Yan-Yu Cui, Yong-Xiang Qin, Qing-Miao Ding and Yu-Ning Gao Tags: Research article Source Type: research
Effectiveness of recombinant Escherichia coli on the production of (R)-(+)-perillyl alcohol
(R)-(+)-perillyl alcohol is a naturally oxygenated monoterpene widely used as the natural flavor additives, insecticides, jet fuels and anti-cancer therapies. It was also readily available monoterpene precursors.... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 8, 2021 Category: Biotechnology Authors: Chao Sun, Xianjuan Dong, Rubing Zhang and Congxia Xie Tags: Research article Source Type: research
Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch cultures
As bioprocess intensification has increased over the last 30 years, yields from mammalian cell processes have increased from 10’s of milligrams to over 10’s of grams per liter. Most of these gains in productiv... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 8, 2021 Category: Biotechnology Authors: Dylan G. Chitwood, Qinghua Wang, Kathryn Elliott, Aiyana Bullock, Dwon Jordana, Zhigang Li, Cathy Wu, Sarah W. Harcum and Christopher A. Saski Tags: Research article Source Type: research
Failure to maintain full-term pregnancies in pig carrying klotho monoallelic knockout fetuses
Small animals that show a deficiency in klotho exhibit extremely shortened life span with multiple aging-like phenotypes. However, limited information is available on the function of klotho in large animals su... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 7, 2021 Category: Biotechnology Authors: Sanghoon Lee, Min Hee Jung, Kilyoung Song, Jun-Xue Jin, Anukul Taweechaipaisankul, Geon A. Kim, Hyun Ju Oh, Ok Jae Koo, Se Chang Park and Byeong Chun Lee Tags: Research article Source Type: research
Establishment of a genetically engineered chicken DF-1 cell line for efficient amplification of influenza viruses in the absence of trypsin
The initial step of influenza infection is binding of the virus to specific sialic acid receptors expressed by host cells. This is followed by cell entry via endocytosis. Cleavage of the influenza virus hemagg... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 7, 2021 Category: Biotechnology Authors: Kelly Chungu, Young Hyun Park, Seung Je Woo, Su Bin Lee, Deivendran Rengaraj, Hong Jo Lee and Jae Yong Han Tags: Research article Source Type: research
Bacillus sp. CSK2 produced thermostable alkaline keratinase using agro-wastes: keratinolytic enzyme characterization
Chicken feathers are the most abundant agro-wastes emanating from the poultry processing farms and present major concerns to environmentalists. Bioutilization of intractable feather wastes for the production o... (Source: BMC Biotechnology)
Source: BMC Biotechnology - December 14, 2020 Category: Biotechnology Authors: Nonso E. Nnolim and Uchechukwu U. Nwodo Tags: Research article Source Type: research
Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production
Lignin in sugarcane bagasse (SB) hinders its utilization by microorganism, therefore, pretreatment methods are employed to make fermentable components accessible to the microbes. Multivariate analysis of diffe... (Source: BMC Biotechnology)
Source: BMC Biotechnology - December 9, 2020 Category: Biotechnology Authors: Rozina Rashid, Uroosa Ejaz, Firdous Imran Ali, Imran Ali Hashmi, Ahmed Bari, Jing Liu, Li Wang, Pengcheng Fu and Muhammad Sohail Tags: Research article Source Type: research
An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibrinolytic enzyme in Komagataella phaffii (Pichia pastoris)
This study aimed to achieve high and efficient pr... (Source: BMC Biotechnology)
Source: BMC Biotechnology - December 4, 2020 Category: Biotechnology Authors: Zhiqun Che, Xiaoyan Cao, Guiguang Chen and Zhiqun Liang Tags: Research article Source Type: research
Reconstitution of Drosophila and human chromatins by wheat germ cell-free co-expression system
Elaboration of the epigenetic regulation of chromatin is a long-standing aim in molecular and cellular biology. Hence, there is a great demand for the development of in vitro methods to reconstitute chromatin ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - December 1, 2020 Category: Biotechnology Authors: Kei-ichi Okimune, Szilvia K. Nagy, Shogo Hataya, Yaeta Endo and Taichi E. Takasuka Tags: Methodology article Source Type: research
Isolation of axenic cyanobacterium and the promoting effect of associated bacterium on axenic cyanobacterium
Harmful cyanobacterial blooms have attracted wide attention all over the world as they cause water quality deterioration and ecosystem health issues. Microcystis aeruginosa associated with a large number of bacte... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 30, 2020 Category: Biotechnology Authors: Suqin Gao, Yun Kong, Jing Yu, Lihong Miao, Lipeng Ji, Lirong Song and Chi Zeng Tags: Research article Source Type: research
