Optimized culture conditions for bacterial cellulose production by Acetobacter senegalensis MA1
Cellulose, the most versatile biomolecule on earth, is available in large quantities from plants. However, cellulose in plants is accompanied by other polymers like hemicellulose, lignin, and pectin. On the ot... (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 26, 2020 Category: Biotechnology Authors: K. Aswini, N. O. Gopal and Sivakumar Uthandi Tags: Research article Source Type: research
Correction to: CRISPR-Cas9 mediated targeted disruption of FAD2 –2 microsomal omega-6 desaturase in soybean (Glycine max.L)
An amendment to this paper has been published and can be accessed via the original article. (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 20, 2020 Category: Biotechnology Authors: Noor al Amin, Naveed Ahmad, Nan Wu, Xiumin Pu, Tong Ma, Yeyao Du, Xiaoxue Bo, Nan Wang, Rahat Sharif and Piwu Wang Tags: Correction Source Type: research
Isolation of novel citrus and plum fruit promoters and their functional characterization for fruit biotechnology
Promoters that confer expression in fruit tissues are important tools for genetic engineering of fruit quality traits, yet few fruit-specific promoters have been identified, particularly for citrus fruit devel... (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 20, 2020 Category: Biotechnology Authors: Kasturi Dasgupta, Sara Hotton, William Belknap, Yasra Syed, Christopher Dardick, Roger Thilmony and James G. Thomson Tags: Research article Source Type: research
Development of Caco-2 cells expressing four CYPs via a mammalian artificial chromosome
Oral administration is the most common way to deliver drugs to the systemic circulation or target organs. Orally administered drugs are absorbed in the intestine and metabolized in the intestine and liver. In ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 20, 2020 Category: Biotechnology Authors: Yumi Ohta, Kanako Kazuki, Satoshi Abe, Mitsuo Oshimura, Kaoru Kobayashi and Yasuhiro Kazuki Tags: Research article Source Type: research
Highly enhanced ELISA sensitivity using acetylated chitosan surfaces
The enzyme-linked immunosorbent assay (ELISA), is the most widely used and reliable clinical routine method for the detection of important protein markers in healthcare. Improving ELISAs is crucial for detecti... (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 19, 2020 Category: Biotechnology Authors: Tania Garc ía-Maceira, Fé I. García-Maceira, José A. González-Reyes and Elier Paz-Rojas Tags: Methodology article Source Type: research
Efficient generation of GGTA1-deficient pigs by electroporation of the CRISPR/Cas9 system into in vitro-fertilized zygotes
Xenoantigens are a major source of concern with regard to the success of interspecific xenografts. GGTA1 encodes α1,3-galactosyltransferase, which is essential for the biosynthesis of galactosyl-alpha 1,3-galacto... (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 18, 2020 Category: Biotechnology Authors: Fuminori Tanihara, Maki Hirata, Nhien Thi Nguyen, Osamu Sawamoto, Takeshi Kikuchi, Masako Doi and Takeshige Otoi Tags: Research article Source Type: research
Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil
With the high demand for diesel across the world, environmental decontamination from its improper usage, storage and accidental spills becomes necessary. One highly environmentally friendly and cost-effective ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - July 25, 2020 Category: Biotechnology Authors: Margaret T. Ho, Michelle S. M. Li, Tim McDowell, Jacqueline MacDonald and Ze-Chun Yuan Tags: Research article Source Type: research
Attenuation of intestinal inflammation in IL-10 deficient mice by a plasmid carrying Lactococcus lactis strain
Inflammatory bowel diseases (IBD) are intestinal disorders characterized by inflammation in the gastrointestinal tract (GIT) and to date, no efficient treatments exist. Interleukin-10 (IL-10), one of the most ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - July 23, 2020 Category: Biotechnology Authors: Meritxell Zurita-Turk, Bianca Mendes Souza, Camila Pr ósperi de Castro, Vanessa Bastos Pereira, Vanessa Pecini da Cunha, Tatiane Melo Preisser, Ana Maria Caetano de Faria, Denise Carmona Cara Machado and Anderson Miyoshi Tags: Research article Source Type: research
One-step heating strategy for efficient solubilization of recombinant spider silk protein from inclusion bodies
Spider silk is a proteinaceous fiber with remarkable mechanical properties spun from spider silk proteins (spidroins). Engineering spidroins have been successfully produced in a variety of heterologous hosts a... (Source: BMC Biotechnology)
Source: BMC Biotechnology - July 10, 2020 Category: Biotechnology Authors: Hui Cai, Gefei Chen, Hairui Yu, Ying Tang, Sidong Xiong and Xingmei Qi Tags: Research article Source Type: research
Functional expression of a novel methanol-stable esterase from Geobacillus subterraneus DSM13552 for biocatalytic synthesis of cinnamyl acetate in a solvent-free system
Esterases are widely distributed in nature and have important applications in medical, industrial and physiological. Recently, the increased demand for flavor esters has prompted the search of catalysts like l... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 29, 2020 Category: Biotechnology Authors: Xianghai Cai, Lin Lin, Yaling Shen, Wei Wei and Dong-zhi Wei Tags: Research article Source Type: research
Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system
Genome editing is transforming bioscience research, but its application to non-model organisms, such as farmed animal species, requires optimisation. Salmonids are the most important aquaculture species by val... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 23, 2020 Category: Biotechnology Authors: Remi L. Gratacap, Tim Regan, Carola E. Dehler, Samuel A. M. Martin, Pierre Boudinot, Bertrand Collet and Ross D. Houston Tags: Methodology article Source Type: research
An improved quantitative real-time polymerase chain reaction technology for Helicobacter pylori detection in stomach tissue and its application value in clinical precision testing
Helicobacter pylori (H. pylori) infection is a serious human health threat. The empiric H. pylori treatment paradigm guided by traditional testing technologies has led to antibiotic resistance. Here, we improved ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 22, 2020 Category: Biotechnology Authors: Ling Deng, Xiao-Yi He, Bin Tang, Yang Xiang and Juan-Juan Yue Tags: Methodology article Source Type: research
Loop-mediated isothermal amplification (LAMP) reaction as viable PCR substitute for diagnostic applications: a comparative analysis study of LAMP, conventional PCR, nested PCR (nPCR) and real-time PCR (qPCR) based on Entamoeba histolytica DNA derived from faecal sample
This study reports the analytical sensitivity and specificity of a Loop-mediated isothermal amplification (LAMP) and compares its amplification performance with conventional PCR, nested PCR (nPCR) and real-tim... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 22, 2020 Category: Biotechnology Authors: Phiaw Chong Foo, A. B. Nurul Najian, Nuramin A. Muhamad, Mariana Ahamad, Maizan Mohamed, Chan Yean Yean and Boon Huat Lim Tags: Research article Source Type: research
Evaluation of Hydra HALT-1 as a toxin moiety for recombinant immunotoxin
Immunotoxin is a hybrid protein consisting of a toxin moiety that is linked to a targeting moiety for the purpose of specific elimination of target cells. Toxins used in traditional immunotoxins are practicall... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 17, 2020 Category: Biotechnology Authors: William F. Jiemy, Lih Fhung Hiew, Hong Xi Sha, Lionel L. A. In and Jung Shan Hwang Tags: Research article Source Type: research
Interference chromatography: a novel approach to optimizing chromatographic selectivity and separation performance for virus purification
Oncolytic viruses are playing an increasingly important role in cancer immunotherapy applications. Given the preclinical and clinical efficacy of these virus-based therapeutics, there is a need for fast, simpl... (Source: BMC Biotechnology)
Source: BMC Biotechnology - June 17, 2020 Category: Biotechnology Authors: Lisa A. Santry, Renaud Jacquemart, Melissa Vandersluis, Mochao Zhao, Jake M. Domm, Thomas M. McAusland, Xiaojiao Shang, Pierre M. Major, James G. Stout and Sarah K. Wootton Tags: Methodology article Source Type: research
