LC-MSMS based screening of emerging pollutant degradation by different peroxidases
The presence of a wide range of bioactive organic pollutants in wastewater and municipal water sources is raising concerns about their potential effects on humans. Not surprisingly, various approaches are bein... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 28, 2019 Category: Biotechnology Authors: Khadega A. Almaqdi, Rana Morsi, Bahia Alhayuti, Farah Alharthi and S. Salman Ashraf Tags: Research article Source Type: research
Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis
In this study, integrative vectors for secret... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 27, 2019 Category: Biotechnology Authors: Innanurdiani Koko, Adelene Ai-Lian Song, Mas Jaffri Masarudin and Raha Abdul Rahim Tags: Research article Source Type: research
A simple method for in vitro preparation of natural killer cells from cord blood
Cord Blood (CB) has been considered a promising source of natural killer (NK) cells for cellular immunotherapy. However, it is difficult to expand the large numbers of highly pure NK cells from CB without cell... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 21, 2019 Category: Biotechnology Authors: Yong Xu Mu, Yu Xia Zhao, Bing Yao Li, Hong Jing Bao, Hui Jiang, Xiao Lei Qi, Li Yun Bai, Yun Hong Wang, Zhi Jie Ma and Xiao Yun Wu Tags: Methodology article Source Type: research
Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein
Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 21, 2019 Category: Biotechnology Authors: Razieh Yazdani, Masoud Shams-Bakhsh, Afshin Hassani-Mehraban, Seyed Shahriar Arab, Nicolas Thelen, Marc Thiry, Jacques Crommen, Marianne Fillet, Nathalie Jacobs, Alain Brans and Anne-Catherine Servais Tags: Research article Source Type: research
Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application
Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When p... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 20, 2019 Category: Biotechnology Authors: Elinea O. Freitas, Bruno P. Melo, Isabela T. Louren ço-Tessutti, Fabrício B. M. Arraes, Regina M. Amorim, Maria E. Lisei-de-Sá, Julia A. Costa, Ana G. B. Leite, Muhammad Faheem, Márcio A. Ferreira, Carolina V. Morgante, Elizabeth P. B. Fontes and Mari Tags: Research article Source Type: research
Isolating promoters from Corynebacterium ammoniagenes ATCC 6871 and application in CoA synthesis
Corynebacterium ammoniagenes is an important industrial organism that is widely used to produce nucleotides and the potential for industrial production of coenzyme A by C. ammoniagenes ATCC 6871 has been shown. H... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 12, 2019 Category: Biotechnology Authors: Yingshuo Hou, Siyu Chen, Jianjun Wang, Guizhen Liu, Sheng Wu and Yong Tao Tags: Research article Source Type: research
Correction to: A new primer construction technique that effectively increases amplification of rare mutant templates in samples
Following publication of the original article [1], the author informed us that the legend for Fig. 2 was incorrect. (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 12, 2019 Category: Biotechnology Authors: Jr-Kai Huang, Ling Fan, Tao-Yeuan Wang and Pao-Shu Wu Tags: Correction Source Type: research
Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element (UCOE)
DNA transposon-based vectors are effective nonviral tools for gene therapy and genetic engineering of cells. However, promoter DNA methylation and a near-random integration profile, which can result in transge... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 9, 2019 Category: Biotechnology Authors: Kristian Alsbjerg Skipper, Anne Kruse Hollensen, Michael N. Antoniou and Jacob Giehm Mikkelsen Tags: Research article Source Type: research
Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects
Genetic mutations cause severe human diseases, and suitable animal models to study the regulatory mechanisms involved are required. The CRISPR/Cas9 system is a powerful, highly efficient and easily manipulated... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 8, 2019 Category: Biotechnology Authors: Guoxing Zheng, Qingqing Zhu, Junchao Dong, Xin Lin and Chengming Zhu Tags: Research article Source Type: research
Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection
Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assa... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 7, 2019 Category: Biotechnology Authors: Aur élie Bak and Joanne B. Emerson Tags: Methodology article Source Type: research
Disruption of a putative mitochondrial oxaloacetate shuttle protein in Aspergillus carbonarius results in secretion of malic acid at the expense of citric acid production
In filamentous fungi, transport of organic acids across the mitochondrial membrane is facilitated by active transport via shuttle proteins. These transporters may transfer different organic acids across the me... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 4, 2019 Category: Biotechnology Authors: Lei Yang, Tore Linde, Abeer H. Hossain, Mette L übeck, Peter J. Punt and Peter S. Lübeck Tags: Research article Source Type: research
gRNA validation for wheat genome editing with the CRISPR-Cas9 system
The CRISPR-Cas9 system is a powerful and versatile tool for crop genome editing. However, achieving highly efficient and specific editing in polyploid species can be a challenge. The efficiency and specificity... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 30, 2019 Category: Biotechnology Authors: Taj Arndell, Niharika Sharma, Peter Langridge, Ute Baumann, Nathan S. Watson-Haigh and Ryan Whitford Tags: Research article Source Type: research
Rapid high throughput template preparation (rHTTP) method: a novel cost effective method of direct PCR for a wide range of plants
Conventional plant DNA isolation methods are complex, time consuming and require technical expertise. These limitations were overcome using the DNA isolation kits which, however significantly add to the resear... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 26, 2019 Category: Biotechnology Authors: Prassan Choudhary, Sudipta Das, Hillol Chakdar, Arjun Singh, Sanjay Kumar Goswami and Anil Kumar Saxena Tags: Methodology article Source Type: research
Use of the kojA promoter, involved in kojic acid biosynthesis, for polyketide production in Aspergillus oryzae: implications for long-term production
Aspergillus oryzae, a useful industrial filamentous fungus, produces limited varieties of secondary metabolites, such as kojic acid. Thus, for the production of valuable secondary metabolites by genetic engineeri... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 26, 2019 Category: Biotechnology Authors: Koichi Tamano, Mahoko Kuninaga, Naoshi Kojima, Myco Umemura, Masayuki Machida and Hideaki Koike Tags: Research article Source Type: research
Standardized mounting method of (zebrafish) embryos using a 3D-printed stamp for high-content, semi-automated confocal imaging
Developmental biology relies to a large extent on the observation and comparison of phenotypic traits through time using high resolution microscopes. In this context, transparent model organisms such as the ze... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 22, 2019 Category: Biotechnology Authors: David Simon Kleinhans and Virginie Lecaudey Tags: Methodology article Source Type: research
