Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate
In industrial microbial biotechnology, fed-batch processes are frequently used to avoid undesirable biological phenomena, such as substrate inhibition or overflow metabolism. For targeted process development, ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - March 2, 2023 Category: Biotechnology Authors: Sarah Sparviero, Laura Barth, Timm Keil, Carl Dinter, Christoph Berg, Clemens Lattermann and Jochen B üchs Tags: Research Source Type: research
In vitro culture of bovine fibroblasts using select serum-free media supplemented with Chlorella vulgaris extract
Standard cell culture practices require the addition of animal-derived serum to culture media to achieve adequate cell growth. Typically, 5 –10% by volume of fetal bovine serum (FBS) is used, which accounts for... (Source: BMC Biotechnology)
Source: BMC Biotechnology - February 8, 2023 Category: Biotechnology Authors: Galileo Defendi-Cho and Timothy M. Gould Tags: Research Source Type: research
Biochemical characterization and insights into the potency of the acidic Aspergillus niger NRC114 purified α-galactosidase in removing raffinose family oligosaccharides from soymilk yogurt
Because humans lack α-galactosidase, foods containing certain oligosaccharides from the raffinose family, such as soybeans and other legumes, may disrupt digestion and cause flatulence. (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 31, 2023 Category: Biotechnology Authors: Abdelmageed M. Othman, Ali M. Elshafei, Maysa A. Elsayed, Gamil E. Ibrahim, Mohamed M. Hassan and Nayra S. Mehanna Tags: Research Source Type: research
Production of rhamnolipid biosurfactants in solid-state fermentation: process optimization and characterization studies
Rhamnolipids are a group of the extracellular microbial surface-active molecules produced by certain Pseudomonas species with various environmental and industrial applications. The goal of the present research wa... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 24, 2023 Category: Biotechnology Authors: Shima Dabaghi, Seyed Ahmad Ataei and Ali Taheri Tags: Research Source Type: research
Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts
Chloroplast transformation is a robust technology for the expression of recombinant proteins. Various types of pharmaceutical proteins including growth factors have been reported in chloroplasts via chloroplas... (Source: BMC Biotechnology)
Source: BMC Biotechnology - January 7, 2023 Category: Biotechnology Authors: Yunpeng Wang, Jieying Fan, Zhengyi Wei and Shaochen Xing Tags: Research Source Type: research
Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design
Docosahexaenoic acid (DHA) can improve human and animal health, particularly including anti-inflammatory, antioxidant, anticancer, neurological, and visual functions. Schizochytrium sp. is a marine heterotrophic ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - December 9, 2022 Category: Biotechnology Authors: Jun Ding, Zilin Fu, Yingkun Zhu, Junhao He, Lu Ma and Dengpan Bu Tags: Research Source Type: research
Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene
Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and dev... (Source: BMC Biotechnology)
Source: BMC Biotechnology - December 6, 2022 Category: Biotechnology Authors: Lei He, Hairong Wang, Zuhua Yu, Chengshui Liao, Ke Ding, Cai Zhang, Chuan Yu and Chunjie Zhang Tags: Research Source Type: research
Correction: Identifcation, expression, and purifcation of DNA cytosine 5-methyltransferases with short recognition sequences
(Source: BMC Biotechnology)
Source: BMC Biotechnology - December 1, 2022 Category: Biotechnology Authors: Fumihito Miura, Miki Miura, Yukiko Shibata, Yoshikazu Furuta, Keisuke Miyamura, Yuki Ino, Asmaa M. A. Bayoumi, Utako Oba and Takashi Ito Tags: Correction Source Type: research
Development of a microbial protease for composting swine carcasses, optimization of its production and elucidation of its catalytic hydrolysis mechanism
Dead swine carcass composting is an excellent method for harmless treatment and resource utilization of swine carcass. However, poor biodegradation ability of traditional composting results in poor harmless tr... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 28, 2022 Category: Biotechnology Authors: Wei Zhai, Xintian Li, Xinran Duan, Changlong Gou, Lixia Wang and Yunhang Gao Tags: Research Source Type: research
Toxic effects of trace phenol/guanidine isothiocyanate (P/GI) on cells cultured nearby in covered 96-well plates
A mixture of phenol and guanidine isothiocyanate ( “P/GI”, the principal components of TRIzol™ and similar products) is routinely used to isolate RNA, DNA, and proteins from a single specimen. In time-course ex... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 25, 2022 Category: Biotechnology Authors: Madeline Snedden, Lavisha Singh, Chandrashekara Kyathanahalli and Emmet Hirsch Tags: Research Source Type: research
Modeling and optimizing in vitro percentage and speed callus induction of carrot via Multilayer Perceptron-Single point discrete GA and radial basis function
Callus induction is the first step in optimizing plant regeneration. Fit embryogenesis and shooting rely on callus induction. In addition, using artificial intelligence models in combination with an algorithm ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 5, 2022 Category: Biotechnology Authors: Masoumeh Fallah Ziarani, Masoud Tohidfar and Mohammad Navvabi Tags: Research Source Type: research
Identification, expression, and purification of DNA cytosine 5-methyltransferases with short recognition sequences
DNA methyltransferases (MTases) are enzymes that induce methylation, one of the representative epigenetic modifications of DNA, and are also useful tools for analyzing epigenomes. However, regarding DNA cytosi... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 4, 2022 Category: Biotechnology Authors: Fumihito Miura, Miki Miura, Yukiko Shibata, Yoshikazu Furuta, Keisuke Miyamura, Yuki Ino, Asmaa M. A. Bayoumi, Utako Oba and Takashi Ito Tags: Research article Source Type: research
Preparation of a novel monoclonal antibody against active components of PHA-L from Phaseolus vulgaris and its functional characteristics
Leukocyte phytohemagglutinin (PHA-L), derived from the L4 tetramer of PHA, has been frequently employed as a mitogen to induce T lymphocyte proliferation in vitro. The biological application of PHA-L in cancer... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 29, 2022 Category: Biotechnology Authors: Peipei Wang, Junmei Hu, Jiaqi Duan, Shitong Min, Congliang Chen, Yue Zhu, Yan Pan, Yitian Wang, Dapeng Wei and Xia Wang Tags: Research Source Type: research
Identification of a novel fully human anti-toxic shock syndrome toxin (TSST)-1 single-chain variable fragment antibody averting TSST-1-induced mitogenesis and cytokine secretion
Staphylococcal superantigens are virulence factors that help the pathogen escape the immune system and develop an infection. Toxic shock syndrome toxin (TSST)-1 is one of the most studied superantigens whose r... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 28, 2022 Category: Biotechnology Authors: Mahdieh Soezi, Somayeh Piri-Gavgani, Mostafa Ghanei, Mir Davood Omrani, Behnoush Soltanmohammadi, Kamran Pooshang Bagheri, Reza Ahangari Cohan, Farzam Vaziri, Seyed Davar Siadat, Abolfazl Fateh, Shohreh Khatami, Masoumeh Azizi and Fatemeh Rahimi-Jamnani Tags: Research Source Type: research
Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24
An important conceptual advance in health and the environment has been recognized that enzymes play a key role in the green processing industries. Of particular interest, chitosanase is beneficial for recyclin... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 27, 2022 Category: Biotechnology Authors: Rong-Xian Zhang, Zhong-Wei Wu, Shu-Juan Zhang, Hui-Min Wei, Cheng-Wei Hua, Lan Li and Tian-You Yang Tags: Research Source Type: research
