Optimization of growth regulators on in vitro propagation of Moringa stenopetala from shoot explants
Moringa stenopetala belongs to the flowering family Moringaceae and genus Moringa. It is often referred to as the East African Moringa tree because it is native only to southern Ethiopia and northern Kenya. The e... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 16, 2020 Category: Biotechnology Authors: Alelegne Yeshamebel Adugna, Tileye Feyissa and Fikresilasie Samuel Tasew Tags: Research article Source Type: research
Comparative gene expression profiling of mouse ovaries upon stimulation with natural equine chorionic gonadotropin (N-eCG) and tethered recombinant-eCG (R-eCG)
Equine chorionic gonadotropin (eCG) induces super-ovulation in laboratory animals. Notwithstanding its extensive usage, limited information is available regarding the differences between the in vivo effects of... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 11, 2020 Category: Biotechnology Authors: Kwan-Sik Min, Jong-Ju Park, So-Yun Lee, Munkhzaya Byambaragchaa and Myung-Hwa Kang Tags: Research article Source Type: research
Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures
Development of precise genome editing strategies is a prerequisite for producing edited plants that can aid in the study of gene function and help understand the genetic traits in a cultivar. Citrus embryogeni... (Source: BMC Biotechnology)
Source: BMC Biotechnology - November 10, 2020 Category: Biotechnology Authors: Manjul Dutt, Zhonglin Mou, Xudong Zhang, Sameena E. Tanwir and Jude W. Grosser Tags: Research article Source Type: research
Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination
Precise genetic modifications are preferred products of CRISPR-Cas9 mediated gene editing in mammalian cells but require the repair of induced double-strand breaks (DSB) through homology directed repair (HDR).... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 23, 2020 Category: Biotechnology Authors: Sanum Bashir, Tu Dang, Jana Rossius, Johanna Wolf and Ralf K ühn Tags: Research article Source Type: research
A novel high performance in-silico screened metagenome-derived alkali-thermostable endo- β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions
Lignocellulosic biomass, is a great resource for the production of bio-energy and bio-based material since it is largely abundant, inexpensive and renewable. The requirement of new energy sources has led to a ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 19, 2020 Category: Biotechnology Authors: Shohreh Ariaeenejad, Atefeh Sheykh Abdollahzadeh Mamaghani, Morteza Maleki, Kaveh Kavousi, Mehdi Foroozandeh Shahraki and Ghasem Hosseini Salekdeh Tags: Research article Source Type: research
Gelatin nanoparticles enable water dispersibility and potentialize the antimicrobial activity of Buriti (Mauritia flexuosa) oil
Buriti oil presents numerous health benefits, but due to its lipophilic nature and high oxidation, it is impossible to incorporate it into aqueous food matrices. Thus, the present study evaluated whether powde... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 16, 2020 Category: Biotechnology Authors: Gabrielle Mahara Martins Azevedo Castro, Thais Souza Passos, Sara Sayonara da Cruz Nascimento, Isaiane Medeiros, Nath ália Kelly Araújo, Bruna Leal Lima Maciel, Carlos Eduardo Padilha, Adriana Margarida Zanbotto Ramalho, Francisco Canidé Sousa Júnior Tags: Research article Source Type: research
Creation of Golden Gate constructs for gene doctoring
Gene doctoring is an efficient recombination-based genetic engineering approach to mutagenesis of the bacterial chromosome that combines the λ-Red recombination system with a suicide donor plasmid that is clea... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 7, 2020 Category: Biotechnology Authors: Nicholas M. Thomson, Chuanzhen Zhang, Eleftheria Trampari and Mark J. Pallen Tags: Research article Source Type: research
Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays
Few bead-based multiplex assays have been described that detect antibodies against the protozoan parasite Toxoplasma gondii in large-scale seroepidemiological surveys. Moreover, each multiplex assay has specific ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 6, 2020 Category: Biotechnology Authors: Sandra Klein, Daniel Stern and Frank Seeber Tags: Methodology article Source Type: research
Kojic acid repurposing as a pancreatic lipase inhibitor and the optimization of its production from a local Aspergillus oryzae soil isolate
Obesity and its related diseases are increasing worldwide. One of the best therapeutic strategies for obesity management is through the inhibition of pancreatic lipase (PL) enzyme. So far orlistat is the only ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - October 2, 2020 Category: Biotechnology Authors: Sarah Mohamed El-Korany, Omneya Mohamed Helmy, Ali Mahmoud El-Halawany, Yasser El-Mohammadi Ragab and Hamdallah Hafez Zedan Tags: Research article Source Type: research
Novel kinase platform for the validation of the anti-tubercular activities of Pelargonium sidoides (Geraniaceae)
Pelargonium sidoides is an important traditional medicine in South Africa with a well-defined history of both traditional and documented use of an aqueous-ethanolic formulation of the roots of P. sidoides (EPs 76... (Source: BMC Biotechnology)
Source: BMC Biotechnology - September 29, 2020 Category: Biotechnology Authors: V. Lukman, S. W. Odeyemi, R. L. Roth, L. Mbabala, N. Tshililo, N. M. Vlok, M. J. B. Dewar and C. P. Kenyon Tags: Research article Source Type: research
In vitro propagation of three mosaic disease resistant cassava cultivars
Cassava is a staple food for over 800 million people globally providing a cheap source of carbohydrate. However, the cultivation of cassava in the country is facing to viral diseases, particularly cassava mosa... (Source: BMC Biotechnology)
Source: BMC Biotechnology - September 29, 2020 Category: Biotechnology Authors: Amitchihou é Franck Sessou, Jane W. Kahia, Jerome Anani Houngue, Elijah Miinda Ateka, Colombe Dadjo and Corneille Ahanhanzo Tags: Research article Source Type: research
Preliminary study of the production of metabolites from in vitro cultures of C. ensiformis
Canavalia ensiformis is a legume native to Central and South America that has historically been a source of protein. Its main proteins, urease, and lectin have been extensively studied and are examples of bioacti... (Source: BMC Biotechnology)
Source: BMC Biotechnology - September 10, 2020 Category: Biotechnology Authors: Juan F. Saldarriaga, Yuby Cruz and Juli án E. López Tags: Research article Source Type: research
Establishing a sensitive fluorescence-based quantification method for cyclic nucleotides
Approximately 40% of prescribed drugs exert their activity via GTP-binding protein-coupled receptors (GPCRs). Once activated, these receptors cause transient changes in the concentration of second messengers, ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 27, 2020 Category: Biotechnology Authors: Nadine Gruteser, Viktoria Kohlhas, Sabine Balfanz, Arne Franzen, Anne G ünther, Andreas Offenhäusser, Frank Müller, Viacheslav Nikolaev, Martin J. Lohse and Arnd Baumann Tags: Methodology article Source Type: research
Synergistic interaction of hTGF- β3 with hBMP-6 promotes articular cartilage formation in chitosan scaffolds with hADSCs: implications for regenerative medicine
Human TGF- β3 has been used in many studies to induce genes coding for typical cartilage matrix components and accelerate chondrogenic differentiation, making it the standard constituent in most cultivation media ... (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 27, 2020 Category: Biotechnology Authors: Yijiang Huang, Daniel Seitz, Yan Chevalier, Peter E. M üller, Volkmar Jansson and Roland M. Klar Tags: Research article Source Type: research
Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents
Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) us... (Source: BMC Biotechnology)
Source: BMC Biotechnology - August 26, 2020 Category: Biotechnology Authors: Young-Hoon Jeong, Ukjin Kim, Seul-Gi Lee, Bokyeong Ryu, Jin Kim, Artyuhov Igor, Jong Soo Kim, Cho-Rok Jung, Jae-Hak Park and C-Yoon Kim Tags: Research article Source Type: research
