A de novo mutation in CACNA1A is associated with autosomal dominant bovine familial convulsions and ataxia in Angus cattle
The objective of this investigation was to identify hypothesized dominant genetic variation underlying the condition. Whole-genome sequencing was performed on the sire, six affected and seven unaffected paternal half-sibling calves and combined with data from 135 unrelated controls. The sire and five of the six affected calves were heterozygous for a nonsense variant (Chr7 g.12367906C>T, c.5073C>T, p.Arg1681*) in CACNA1A. The other affected calves (N = 8) were heterozygous for the variant but it was absent in the other unaffected calves (N = 7) and parents of the sire. This variant was also absent in sequence data fr...
Source: Animal Genetics - March 1, 2024 Category: Genetics & Stem Cells Authors: Rachel R Reith Jonathan E Beever Joe C Paschal Jason Banta Brian F Porter David J Steffen Thomas B Hairgrove Jessica L Petersen Source Type: research
The role of genetic variability of the host on the resistance to Neospora caninum infection in cattle
Anim Genet. 2024 Feb 28. doi: 10.1111/age.13410. Online ahead of print.ABSTRACTNeospora caninum is one of the most frequently diagnosed abortifacient pathogens in cattle. There is abundant genomic information about the parasite itself, but very little is known about the genetic variability of resistance in the most common intermediate host. The aim of this review was to compile all the available information about the genetic variability associated with the resistance to N. caninum both between and within cattle breeds. We systematically searched for published studies that investigated the influence of genetics of the host ...
Source: Animal Genetics - February 29, 2024 Category: Genetics & Stem Cells Authors: Anabella Dinon Franco Fiorani Luc ía María Campero Dad ín Prando Moore Pablo Marcelo Corva Source Type: research
The role of genetic variability of the host on the resistance to Neospora caninum infection in cattle
Anim Genet. 2024 Feb 28. doi: 10.1111/age.13410. Online ahead of print.ABSTRACTNeospora caninum is one of the most frequently diagnosed abortifacient pathogens in cattle. There is abundant genomic information about the parasite itself, but very little is known about the genetic variability of resistance in the most common intermediate host. The aim of this review was to compile all the available information about the genetic variability associated with the resistance to N. caninum both between and within cattle breeds. We systematically searched for published studies that investigated the influence of genetics of the host ...
Source: Animal Genetics - February 29, 2024 Category: Genetics & Stem Cells Authors: Anabella Dinon Franco Fiorani Luc ía María Campero Dad ín Prando Moore Pablo Marcelo Corva Source Type: research
Evaluation of the effectiveness of single nucleotide polymorphisms compared to microsatellite markers for parentage verification in Moroccan horses
In this study, 1074 horses were genotyped using the 144 SNPs panel, including 432 foals, 414 mares, and 228 stallions, from five different breeds: 293 Arabians, 167 Barbs, 189 Thoroughbreds, 73 Anglo-Arabians, and 352 Arabian-Barbs. As a result, two SNPs markers were eliminated from the panel system due to inconsistent amplification across all examined individuals leaving 142 SNPs markers for analysis. A comparative analysis between SNPs and STRs markers revealed that the mean expected heterozygosity was 0.457 for SNPs and 0.76 for STRs, while the mean observed heterozygosity stood at 0.472 for SNPs and 0.72 for STRs. Furt...
Source: Animal Genetics - February 22, 2024 Category: Genetics & Stem Cells Authors: O Aminou B Badaoui M Machmoum M Piro Source Type: research
Evaluation of the effectiveness of single nucleotide polymorphisms compared to microsatellite markers for parentage verification in Moroccan horses
In this study, 1074 horses were genotyped using the 144 SNPs panel, including 432 foals, 414 mares, and 228 stallions, from five different breeds: 293 Arabians, 167 Barbs, 189 Thoroughbreds, 73 Anglo-Arabians, and 352 Arabian-Barbs. As a result, two SNPs markers were eliminated from the panel system due to inconsistent amplification across all examined individuals leaving 142 SNPs markers for analysis. A comparative analysis between SNPs and STRs markers revealed that the mean expected heterozygosity was 0.457 for SNPs and 0.76 for STRs, while the mean observed heterozygosity stood at 0.472 for SNPs and 0.72 for STRs. Furt...
Source: Animal Genetics - February 22, 2024 Category: Genetics & Stem Cells Authors: O Aminou B Badaoui M Machmoum M Piro Source Type: research
Evaluation of the effectiveness of single nucleotide polymorphisms compared to microsatellite markers for parentage verification in Moroccan horses
In this study, 1074 horses were genotyped using the 144 SNPs panel, including 432 foals, 414 mares, and 228 stallions, from five different breeds: 293 Arabians, 167 Barbs, 189 Thoroughbreds, 73 Anglo-Arabians, and 352 Arabian-Barbs. As a result, two SNPs markers were eliminated from the panel system due to inconsistent amplification across all examined individuals leaving 142 SNPs markers for analysis. A comparative analysis between SNPs and STRs markers revealed that the mean expected heterozygosity was 0.457 for SNPs and 0.76 for STRs, while the mean observed heterozygosity stood at 0.472 for SNPs and 0.72 for STRs. Furt...
Source: Animal Genetics - February 22, 2024 Category: Genetics & Stem Cells Authors: O Aminou B Badaoui M Machmoum M Piro Source Type: research
Evaluation of the effectiveness of single nucleotide polymorphisms compared to microsatellite markers for parentage verification in Moroccan horses
In this study, 1074 horses were genotyped using the 144 SNPs panel, including 432 foals, 414 mares, and 228 stallions, from five different breeds: 293 Arabians, 167 Barbs, 189 Thoroughbreds, 73 Anglo-Arabians, and 352 Arabian-Barbs. As a result, two SNPs markers were eliminated from the panel system due to inconsistent amplification across all examined individuals leaving 142 SNPs markers for analysis. A comparative analysis between SNPs and STRs markers revealed that the mean expected heterozygosity was 0.457 for SNPs and 0.76 for STRs, while the mean observed heterozygosity stood at 0.472 for SNPs and 0.72 for STRs. Furt...
Source: Animal Genetics - February 22, 2024 Category: Genetics & Stem Cells Authors: O Aminou B Badaoui M Machmoum M Piro Source Type: research
Evaluation of the effectiveness of single nucleotide polymorphisms compared to microsatellite markers for parentage verification in Moroccan horses
In this study, 1074 horses were genotyped using the 144 SNPs panel, including 432 foals, 414 mares, and 228 stallions, from five different breeds: 293 Arabians, 167 Barbs, 189 Thoroughbreds, 73 Anglo-Arabians, and 352 Arabian-Barbs. As a result, two SNPs markers were eliminated from the panel system due to inconsistent amplification across all examined individuals leaving 142 SNPs markers for analysis. A comparative analysis between SNPs and STRs markers revealed that the mean expected heterozygosity was 0.457 for SNPs and 0.76 for STRs, while the mean observed heterozygosity stood at 0.472 for SNPs and 0.72 for STRs. Furt...
Source: Animal Genetics - February 22, 2024 Category: Genetics & Stem Cells Authors: O Aminou B Badaoui M Machmoum M Piro Source Type: research
Evaluation of the effectiveness of single nucleotide polymorphisms compared to microsatellite markers for parentage verification in Moroccan horses
In this study, 1074 horses were genotyped using the 144 SNPs panel, including 432 foals, 414 mares, and 228 stallions, from five different breeds: 293 Arabians, 167 Barbs, 189 Thoroughbreds, 73 Anglo-Arabians, and 352 Arabian-Barbs. As a result, two SNPs markers were eliminated from the panel system due to inconsistent amplification across all examined individuals leaving 142 SNPs markers for analysis. A comparative analysis between SNPs and STRs markers revealed that the mean expected heterozygosity was 0.457 for SNPs and 0.76 for STRs, while the mean observed heterozygosity stood at 0.472 for SNPs and 0.72 for STRs. Furt...
Source: Animal Genetics - February 22, 2024 Category: Genetics & Stem Cells Authors: O Aminou B Badaoui M Machmoum M Piro Source Type: research
Phenotypic correlations of carpal gland diverticular number with production traits and its genome-wide association analysis in multiple pig populations
In this study, 1028 pigs from six populations were counted for the carpal gland diverticular numbers (CGDNs) on the left (CGDNL ) and right (CGDNR ) legs, and their carcass and meat quality traits were assessed. The CGDNs were significantly different among the populations, and Licha Black pigs had a lower CGDN than the Bama Xiang breed. It was also significantly different between sexes, with males having more diverticula than females (p ≤ 0.0391). Moreover, the number was asymmetric, with CGDNR being significantly higher than CGDNL . Notably, CGDNs was significantly correlated with each other in phenotype and genetics an...
Source: Animal Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Dengshuai Cui Longyun Li Naibiao Yu Sanya Xiong Shijun Xiao Hao Zheng Zhiyong Huang Yuanmei Guo Lusheng Huang Source Type: research
Phenotypic correlations of carpal gland diverticular number with production traits and its genome-wide association analysis in multiple pig populations
In this study, 1028 pigs from six populations were counted for the carpal gland diverticular numbers (CGDNs) on the left (CGDNL ) and right (CGDNR ) legs, and their carcass and meat quality traits were assessed. The CGDNs were significantly different among the populations, and Licha Black pigs had a lower CGDN than the Bama Xiang breed. It was also significantly different between sexes, with males having more diverticula than females (p ≤ 0.0391). Moreover, the number was asymmetric, with CGDNR being significantly higher than CGDNL . Notably, CGDNs was significantly correlated with each other in phenotype and genetics an...
Source: Animal Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Dengshuai Cui Longyun Li Naibiao Yu Sanya Xiong Shijun Xiao Hao Zheng Zhiyong Huang Yuanmei Guo Lusheng Huang Source Type: research
LncRNA ENSGALG00000021686 regulates fat metabolism in chicken hepatocytes via miR-146b/AGPAT2 pathway
Anim Genet. 2024 Feb 18. doi: 10.1111/age.13405. Online ahead of print.ABSTRACTThe liver contributes to lipid metabolism as the hub of fat synthesis. Long non-coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes' lipid metabolism. Thirty-two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR-146b along with the lev...
Source: Animal Genetics - February 19, 2024 Category: Genetics & Stem Cells Authors: Wenhao Xing Shijie Li Source Type: research
LncRNA ENSGALG00000021686 regulates fat metabolism in chicken hepatocytes via miR-146b/AGPAT2 pathway
Anim Genet. 2024 Feb 18. doi: 10.1111/age.13405. Online ahead of print.ABSTRACTThe liver contributes to lipid metabolism as the hub of fat synthesis. Long non-coding RNAs (lncRNAs) are considered the regulators of cellular processes. Since LncRNA ENSGALG00000021686 (lncRNA 21 686) has been described as a regulator of lipid metabolism, the present study aimed to clarify the role of lncRNA 21 686 in chicken hepatocytes' lipid metabolism. Thirty-two chickens were divided into four groups and were treated with diets containing different amounts of fat, and the hepatic expression of lncRNA 21 686 and miR-146b along with the lev...
Source: Animal Genetics - February 19, 2024 Category: Genetics & Stem Cells Authors: Wenhao Xing Shijie Li Source Type: research
Comparison of two methods of sperm- and testis-mediated gene transfer in production of transgenic animals: A systematic review
Anim Genet. 2024 Feb 15. doi: 10.1111/age.13404. Online ahead of print.ABSTRACTTransgenic (Tg) animal technology is one of the growing areas in biology. Various Tg technologies, each with its own advantages and disadvantages, are available for generating Tg animals. These include zygote microinjection, electroporation, viral infection, embryonic stem cell or spermatogonial stem cell-mediated production of Tg animals, sperm-mediated gene transfer (SMGT), and testis-mediated gene transfer (TMGT). However, there are currently no comprehensive studies comparing SMGT and TMGT methods, selecting appropriate gene delivery carrier...
Source: Animal Genetics - February 16, 2024 Category: Genetics & Stem Cells Authors: Zeinab Dehghan Gholamhossein Darya Shayesteh Mehdinejadiani Amin Derakhshanfar Source Type: research
Comparison of two methods of sperm- and testis-mediated gene transfer in production of transgenic animals: A systematic review
Anim Genet. 2024 Feb 15. doi: 10.1111/age.13404. Online ahead of print.ABSTRACTTransgenic (Tg) animal technology is one of the growing areas in biology. Various Tg technologies, each with its own advantages and disadvantages, are available for generating Tg animals. These include zygote microinjection, electroporation, viral infection, embryonic stem cell or spermatogonial stem cell-mediated production of Tg animals, sperm-mediated gene transfer (SMGT), and testis-mediated gene transfer (TMGT). However, there are currently no comprehensive studies comparing SMGT and TMGT methods, selecting appropriate gene delivery carrier...
Source: Animal Genetics - February 16, 2024 Category: Genetics & Stem Cells Authors: Zeinab Dehghan Gholamhossein Darya Shayesteh Mehdinejadiani Amin Derakhshanfar Source Type: research
