Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination
Acta Histochem. 2023 Sep 15;125(7):152092. doi: 10.1016/j.acthis.2023.152092. Online ahead of print.ABSTRACTIn maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase c...
Source: Acta Histochemica - September 17, 2023 Category: Biochemistry Authors: Diana I Romero-S ánchez Sonia V ázquez-Santana Rafael A Alonso-Alvarez Jorge M V ázquez-Ramos Aurora Lara-N úñez Source Type: research

Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination
Acta Histochem. 2023 Sep 15;125(7):152092. doi: 10.1016/j.acthis.2023.152092. Online ahead of print.ABSTRACTIn maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase c...
Source: Acta Histochemica - September 17, 2023 Category: Biochemistry Authors: Diana I Romero-S ánchez Sonia V ázquez-Santana Rafael A Alonso-Alvarez Jorge M V ázquez-Ramos Aurora Lara-N úñez Source Type: research

Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination
Acta Histochem. 2023 Sep 15;125(7):152092. doi: 10.1016/j.acthis.2023.152092. Online ahead of print.ABSTRACTIn maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase c...
Source: Acta Histochemica - September 17, 2023 Category: Biochemistry Authors: Diana I Romero-S ánchez Sonia V ázquez-Santana Rafael A Alonso-Alvarez Jorge M V ázquez-Ramos Aurora Lara-N úñez Source Type: research

Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination
Acta Histochem. 2023 Sep 15;125(7):152092. doi: 10.1016/j.acthis.2023.152092. Online ahead of print.ABSTRACTIn maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase c...
Source: Acta Histochemica - September 17, 2023 Category: Biochemistry Authors: Diana I Romero-S ánchez Sonia V ázquez-Santana Rafael A Alonso-Alvarez Jorge M V ázquez-Ramos Aurora Lara-N úñez Source Type: research

Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination
Acta Histochem. 2023 Sep 15;125(7):152092. doi: 10.1016/j.acthis.2023.152092. Online ahead of print.ABSTRACTIn maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase c...
Source: Acta Histochemica - September 17, 2023 Category: Biochemistry Authors: Diana I Romero-S ánchez Sonia V ázquez-Santana Rafael A Alonso-Alvarez Jorge M V ázquez-Ramos Aurora Lara-N úñez Source Type: research

Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination
Acta Histochem. 2023 Sep 15;125(7):152092. doi: 10.1016/j.acthis.2023.152092. Online ahead of print.ABSTRACTIn maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase c...
Source: Acta Histochemica - September 17, 2023 Category: Biochemistry Authors: Diana I Romero-S ánchez Sonia V ázquez-Santana Rafael A Alonso-Alvarez Jorge M V ázquez-Ramos Aurora Lara-N úñez Source Type: research

Tissue and subcellular localization of CycD2 and KRPs are dissimilarly distributed by glucose and sucrose during early maize germination
Acta Histochem. 2023 Sep 15;125(7):152092. doi: 10.1016/j.acthis.2023.152092. Online ahead of print.ABSTRACTIn maize, immunoprecipitation assays have shown that CycD2;2 interacts with KRPs. However, evidence on CycD2;2 or KRPs localization and their possible interaction in specific tissues is lacking and its physiological consequence is still unknown. This work explores the spatiotemporal presence of CyclinD2s and KRPs, cell cycle regulators, during maize seed germination (18 and 36 h) after soaking on glucose or sucrose (120 mM). CyclinD2s are positive actors driving proliferation; KRPs are inhibitors of the main kinase c...
Source: Acta Histochemica - September 17, 2023 Category: Biochemistry Authors: Diana I Romero-S ánchez Sonia V ázquez-Santana Rafael A Alonso-Alvarez Jorge M V ázquez-Ramos Aurora Lara-N úñez Source Type: research

Immunolocalization of Cytoplasmic ER in ER-negative Breast Carcinoma as a Potent Favorable Prognostic Predictor
Acta Histochem Cytochem. 2023 Aug 30;56(4):59-66. doi: 10.1267/ahc.23-00016. Epub 2023 Aug 23.ABSTRACTIt is known that estrogen receptor (ER) has extranuclear signaling functions in addition to classical genomic pathway, and estrogenic actions have been reported in ER-negative breast carcinoma cells. However, significance of cytoplasmic-ER immunoreactivity has not been reported in ER-negative breast carcinoma tissues. We immunolocalized cytoplasmic ER in 155 ER-negative breast carcinoma tissues and evaluated its clinicopathological significance including the prognosis. As a comparative cohort set, we also used 142 ER-posit...
Source: Acta Histochemica - September 8, 2023 Category: Biochemistry Authors: Akiko Ebata Takashi Suzuki Narumi Shoji-Harada Yohei Hamanaka Minoru Miyashita Erina Iwabuchi Kiyoshi Takagi Yasuhiro Miki Hiroshi Tada Takanori Ishida Source Type: research

Muscarinic Receptor Stimulation Does Not Inhibit Voltage-dependent Ca < sup > 2+ < /sup > Channels in Rat Adrenal Medullary Chromaffin Cells
Acta Histochem Cytochem. 2023 Aug 30;56(4):67-75. doi: 10.1267/ahc.23-00042. Epub 2023 Aug 23.ABSTRACTAdrenal medullary chromaffin (AMC) and sympathetic ganglion cells are derived from the neural crest and show a similar developmental path. Thus, these two cell types have many common properties in membrane excitability and signaling. However, AMC cells function as endocrine cells while sympathetic ganglion cells are neurons. In rat sympathetic ganglion cells, muscarinic M1 and M4 receptors mediate excitation and inhibition via suppression of M-type K+ channels and suppression of voltage-dependent Ca2+ channels, respectivel...
Source: Acta Histochemica - September 8, 2023 Category: Biochemistry Authors: Keita Harada Masumi Inoue Source Type: research

Immunolocalization of Cytoplasmic ER in ER-negative Breast Carcinoma as a Potent Favorable Prognostic Predictor
Acta Histochem Cytochem. 2023 Aug 30;56(4):59-66. doi: 10.1267/ahc.23-00016. Epub 2023 Aug 23.ABSTRACTIt is known that estrogen receptor (ER) has extranuclear signaling functions in addition to classical genomic pathway, and estrogenic actions have been reported in ER-negative breast carcinoma cells. However, significance of cytoplasmic-ER immunoreactivity has not been reported in ER-negative breast carcinoma tissues. We immunolocalized cytoplasmic ER in 155 ER-negative breast carcinoma tissues and evaluated its clinicopathological significance including the prognosis. As a comparative cohort set, we also used 142 ER-posit...
Source: Acta Histochemica - September 8, 2023 Category: Biochemistry Authors: Akiko Ebata Takashi Suzuki Narumi Shoji-Harada Yohei Hamanaka Minoru Miyashita Erina Iwabuchi Kiyoshi Takagi Yasuhiro Miki Hiroshi Tada Takanori Ishida Source Type: research

Muscarinic Receptor Stimulation Does Not Inhibit Voltage-dependent Ca < sup > 2+ < /sup > Channels in Rat Adrenal Medullary Chromaffin Cells
Acta Histochem Cytochem. 2023 Aug 30;56(4):67-75. doi: 10.1267/ahc.23-00042. Epub 2023 Aug 23.ABSTRACTAdrenal medullary chromaffin (AMC) and sympathetic ganglion cells are derived from the neural crest and show a similar developmental path. Thus, these two cell types have many common properties in membrane excitability and signaling. However, AMC cells function as endocrine cells while sympathetic ganglion cells are neurons. In rat sympathetic ganglion cells, muscarinic M1 and M4 receptors mediate excitation and inhibition via suppression of M-type K+ channels and suppression of voltage-dependent Ca2+ channels, respectivel...
Source: Acta Histochemica - September 8, 2023 Category: Biochemistry Authors: Keita Harada Masumi Inoue Source Type: research

Immunolocalization of Cytoplasmic ER in ER-negative Breast Carcinoma as a Potent Favorable Prognostic Predictor
Acta Histochem Cytochem. 2023 Aug 30;56(4):59-66. doi: 10.1267/ahc.23-00016. Epub 2023 Aug 23.ABSTRACTIt is known that estrogen receptor (ER) has extranuclear signaling functions in addition to classical genomic pathway, and estrogenic actions have been reported in ER-negative breast carcinoma cells. However, significance of cytoplasmic-ER immunoreactivity has not been reported in ER-negative breast carcinoma tissues. We immunolocalized cytoplasmic ER in 155 ER-negative breast carcinoma tissues and evaluated its clinicopathological significance including the prognosis. As a comparative cohort set, we also used 142 ER-posit...
Source: Acta Histochemica - September 8, 2023 Category: Biochemistry Authors: Akiko Ebata Takashi Suzuki Narumi Shoji-Harada Yohei Hamanaka Minoru Miyashita Erina Iwabuchi Kiyoshi Takagi Yasuhiro Miki Hiroshi Tada Takanori Ishida Source Type: research

Muscarinic Receptor Stimulation Does Not Inhibit Voltage-dependent Ca < sup > 2+ < /sup > Channels in Rat Adrenal Medullary Chromaffin Cells
Acta Histochem Cytochem. 2023 Aug 30;56(4):67-75. doi: 10.1267/ahc.23-00042. Epub 2023 Aug 23.ABSTRACTAdrenal medullary chromaffin (AMC) and sympathetic ganglion cells are derived from the neural crest and show a similar developmental path. Thus, these two cell types have many common properties in membrane excitability and signaling. However, AMC cells function as endocrine cells while sympathetic ganglion cells are neurons. In rat sympathetic ganglion cells, muscarinic M1 and M4 receptors mediate excitation and inhibition via suppression of M-type K+ channels and suppression of voltage-dependent Ca2+ channels, respectivel...
Source: Acta Histochemica - September 8, 2023 Category: Biochemistry Authors: Keita Harada Masumi Inoue Source Type: research

Immunolocalization of Cytoplasmic ER in ER-negative Breast Carcinoma as a Potent Favorable Prognostic Predictor
Acta Histochem Cytochem. 2023 Aug 30;56(4):59-66. doi: 10.1267/ahc.23-00016. Epub 2023 Aug 23.ABSTRACTIt is known that estrogen receptor (ER) has extranuclear signaling functions in addition to classical genomic pathway, and estrogenic actions have been reported in ER-negative breast carcinoma cells. However, significance of cytoplasmic-ER immunoreactivity has not been reported in ER-negative breast carcinoma tissues. We immunolocalized cytoplasmic ER in 155 ER-negative breast carcinoma tissues and evaluated its clinicopathological significance including the prognosis. As a comparative cohort set, we also used 142 ER-posit...
Source: Acta Histochemica - September 8, 2023 Category: Biochemistry Authors: Akiko Ebata Takashi Suzuki Narumi Shoji-Harada Yohei Hamanaka Minoru Miyashita Erina Iwabuchi Kiyoshi Takagi Yasuhiro Miki Hiroshi Tada Takanori Ishida Source Type: research

Muscarinic Receptor Stimulation Does Not Inhibit Voltage-dependent Ca < sup > 2+ < /sup > Channels in Rat Adrenal Medullary Chromaffin Cells
Acta Histochem Cytochem. 2023 Aug 30;56(4):67-75. doi: 10.1267/ahc.23-00042. Epub 2023 Aug 23.ABSTRACTAdrenal medullary chromaffin (AMC) and sympathetic ganglion cells are derived from the neural crest and show a similar developmental path. Thus, these two cell types have many common properties in membrane excitability and signaling. However, AMC cells function as endocrine cells while sympathetic ganglion cells are neurons. In rat sympathetic ganglion cells, muscarinic M1 and M4 receptors mediate excitation and inhibition via suppression of M-type K+ channels and suppression of voltage-dependent Ca2+ channels, respectivel...
Source: Acta Histochemica - September 8, 2023 Category: Biochemistry Authors: Keita Harada Masumi Inoue Source Type: research