Immunolocalization patterns of histone-deacetylases in salivary glands of mice during postnatal development
CONCLUSIONS: This study has provided an important reference for understanding the spatiotemporal-specific expression of HDACs in the salivary gland. These results offer new clues for the experimenters and hold promise for developing innovative therapeutic strategies for salivary gland-related diseases.PMID:38382218 | DOI:10.1016/j.acthis.2024.152144 (Source: Acta Histochemica)
Source: Acta Histochemica - February 21, 2024 Category: Biochemistry Authors: Chubo Yang Xuejing Song Jiaqi Kong Huishu Li Yuanbo Zhan Source Type: research
Sialylation status and its relationship with morphofunctional changes in human adult testis during sexually mature life and aging: A narrative review
Acta Histochem. 2024 Feb 20;126(2):152143. doi: 10.1016/j.acthis.2024.152143. Online ahead of print.ABSTRACTSialic acids (Sias) are a family of electronegatively charged nine-carbon monosaccharides containing a carboxylic acid, mostly found as terminal residues in glycans of glycoproteins and glycolipids. They are bound to galactose or N-acetylgalactosamine via α2,3 or α2,6 linkage, or to other Sias especially via α2,8 linkage, which results in monomeric, oligomeric, and polymeric forms. Sias play determinant roles in a multitude of biological processes in human tissues from development to adult life until aging. In thi...
Source: Acta Histochemica - February 21, 2024 Category: Biochemistry Authors: Mirko Manetti Mirca Marini Angelica Perna Alessia Tani Eleonora Sgambati Source Type: research
Analysis of Insulinoma-Associated Protein 1 Expression in Pituitary Neuroendocrine Tumors
This study aimed to examine the expression of INSM1 using immunohistochemistry, in the various cell lineages of PitNET classified by hormone secretion and transcription factor expression. INSM1 expression in PitNETs (different subtypes) and normal pituitary tissues was immunohistochemically assessed. The results were interpreted as scores of 0 (negative), 1 (focally positive), or 2 (frankly positive), depending on the proportion of cell staining. Twenty-eight of 35 PitNET cases (80%) showed INSM1 positivity in their nuclei. The staining in each histological subtype of PitNETs was as follows: somatotroph tumors, score 0 = 3...
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Yu Hirokawa Chie Inomoto Kenichi Oyama Shigeyuki Tahara Robert Y Osamura Takayuki Shiomi Akira Matsuno Source Type: research
Erratum: Histomorphological Changes in a Rat Model of Polycystic Ovary Syndrome and the Contribution of Stevia Leaf Extract in Modulating the Ovarian Fibrosis, VEGF, and TGF-beta Immunoexpressions: Comparison with Metformin [Acta Histochem. Cytochem. 55, 9-23 (2022)]
Acta Histochem Cytochem. 2023 Dec 28;56(6):153. doi: 10.1267/ahc.21-00081E.ABSTRACT[This corrects the article DOI: 10.1267/ahc.21-00081.].PMID:38318100 | PMC:PMC10838631 | DOI:10.1267/ahc.21-00081E (Source: Acta Histochemica)
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Source Type: research
A Single Administration of Progesterone during the Neonatal Period Shows No Structural Changes in Male Reproductive Tracts in Mice
This study suggests that progesterone treatment in neonatal mice does not cause any structural changes in the male reproductive tracts at puberty, unlike the neonatal estrogen treatment.PMID:38318101 | PMC:PMC10838630 | DOI:10.1267/ahc.23-00052 (Source: Acta Histochemica)
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Takuya Omotehara Hiroki Nakata Kenta Nagahori Miyuki Kuramasu Koichiro Ichimura Masahiro Itoh Source Type: research
Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload
Acta Histochem Cytochem. 2023 Dec 28;56(6):111-119. doi: 10.1267/ahc.23-00041. Epub 2023 Dec 20.ABSTRACTMyogenic cell differentiation is modulated by multiple regulatory factors, such as myogenin, p21, and cyclin D3 during myogenesis in vitro. It is also recognized that myogenin and p21 play important roles in regulating muscle satellite cell (SC) differentiation during overload-induced muscle hypertrophy in vivo. However, the expression patterns and functional role of cyclin D3 in the progress of muscle hypertrophy remain unclear. Thus, the present study investigated cyclin D3 expression in skeletal muscles during early-s...
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Minenori Ishido Source Type: research
Visualization of Amyloid Oligomers in the Brain of Patients with Alzheimer's Disease
Acta Histochem Cytochem. 2023 Dec 28;56(6):87-94. doi: 10.1267/ahc.23-00058. Epub 2023 Dec 20.ABSTRACTIn the pathogenesis of Alzheimer's disease (AD), highly neurotoxic amyloid-β (Aβ) oligomers appear early, they are thus considered to be deeply involved in the onset of Alzheimer's disease. However, Aβ oligomer visualization is challenging in human tissues due to their multiple forms (e.g., low- and high-molecular-weight oligomers, including protofibrils) as well as their tendency to rapidly change forms and aggregate. In this review, we present two visualization approaches for Aβ oligomers in tissues: an immunohistoch...
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Ikuo Tooyama Tomoko Kato Hiroyasu Taguchi Yusuke Kageyama Kazuhiro Irie Yukie Hirahara Daijiro Yanagisawa Source Type: research
Distribution of Contractile Structures in a Mouse and Human Lymph Node Associated with Lymph Flow
Acta Histochem Cytochem. 2023 Dec 28;56(6):137-144. doi: 10.1267/ahc.23-00054. Epub 2023 Dec 23.ABSTRACTLymph nodes have contractile structures, but their distribution in a lymph node has been less considered in terms of facilitation of lymph flow. Axillary, inguinal, and mesenteric lymph nodes were collected from mice and human cadavers, and their sections were immunostained for alpha-smooth muscle actin (αSMA) and high molecular weight caldesmon (H-caldesmon). The αSMA-positive cells were localized in the capsule beneath the ceiling epithelium on the afferent side in both mice and humans. We found an additional layer o...
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Yasuhisa Ishi Takuya Omotehara Xi Wu Miyuki Kuramasu Shinichi Kawata Masahiro Itoh Source Type: research
High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats
Acta Histochem Cytochem. 2023 Dec 28;56(6):95-104. doi: 10.1267/ahc.23-00029. Epub 2023 Dec 20.ABSTRACTProlonged inactivity in skeletal muscles decreases muscle capillary development because of an imbalance between pro- and antiangiogenic signals, mitochondrial metabolism disorders, and increased oxidative stress. Nucleotides have been shown to exert a dose-dependent effect on disuse-induced muscle atrophy. However, the dose-dependent effect on capillary regression in disused muscles remains unclear. Therefore, this study investigated the dose-dependent effect of nucleotides on capillary regression due to disuse. For this ...
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Ryosuke Nakanishi Nagisa Hashimoto Miho Takuwa Jihao Xing Mikiko Uemura Badur Un Nisa Masayuki Tanaka Takumi Hirabayashi Minoru Tanaka Hidemi Fujino Source Type: research
Immunohistochemical Analyses of Mammalian Target of Rapamycin (mTOR) Expression in Pituitary Neuroendocrine Tumors (PitNETs): mTOR as a Therapeutic Target for Functional PitNETs
Acta Histochem Cytochem. 2023 Dec 28;56(6):121-126. doi: 10.1267/ahc.23-00039. Epub 2023 Dec 20.ABSTRACTCurrent therapeutic modalities for pituitary neuroendocrine tumors (PitNETs) include medication, surgery, and radiotherapy. Some patients have tumors that are refractory to current modalities. Therefore, novel treatment options are needed for patients with intractable diseases. Consequently, we examined the pathological data of PitNETs to study medical therapies. We retrospectively studied 120 patients with histologically diagnosed PitNETs. We used the data for the histopathological examination of hormones, such as growt...
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Ichiro Nakazato Takayuki Shiomi Kenichi Oyama Akira Matsuno Chie Inomoto R Yoshiyuki Osamura Source Type: research
Localization of Sites of Osimertinib Action in Rat Intestine, Skin, and Lung by Immunohistochemistry
This report is the first to elucidate the localization of the sites of action of osimertinib in the rat intestine, skin, and lung and is expected to help clarify the mechanism of osimertinib-induced adverse effects.PMID:38318107 | PMC:PMC10838634 | DOI:10.1267/ahc.23-00055 (Source: Acta Histochemica)
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Yuta Yamamoto Tetsuya Saita Hiroto Kataoka Rintaro Sogawa Sakiko Kimura Shinya Kimura Chisato Shimanoe Masashi Shin Source Type: research
Analysis of Insulinoma-Associated Protein 1 Expression in Pituitary Neuroendocrine Tumors
This study aimed to examine the expression of INSM1 using immunohistochemistry, in the various cell lineages of PitNET classified by hormone secretion and transcription factor expression. INSM1 expression in PitNETs (different subtypes) and normal pituitary tissues was immunohistochemically assessed. The results were interpreted as scores of 0 (negative), 1 (focally positive), or 2 (frankly positive), depending on the proportion of cell staining. Twenty-eight of 35 PitNET cases (80%) showed INSM1 positivity in their nuclei. The staining in each histological subtype of PitNETs was as follows: somatotroph tumors, score 0 = 3...
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Yu Hirokawa Chie Inomoto Kenichi Oyama Shigeyuki Tahara Robert Y Osamura Takayuki Shiomi Akira Matsuno Source Type: research
Erratum: Histomorphological Changes in a Rat Model of Polycystic Ovary Syndrome and the Contribution of Stevia Leaf Extract in Modulating the Ovarian Fibrosis, VEGF, and TGF-beta Immunoexpressions: Comparison with Metformin [Acta Histochem. Cytochem. 55, 9-23 (2022)]
Acta Histochem Cytochem. 2023 Dec 28;56(6):153. doi: 10.1267/ahc.21-00081E.ABSTRACT[This corrects the article DOI: 10.1267/ahc.21-00081.].PMID:38318100 | PMC:PMC10838631 | DOI:10.1267/ahc.21-00081E (Source: Acta Histochemica)
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Source Type: research
A Single Administration of Progesterone during the Neonatal Period Shows No Structural Changes in Male Reproductive Tracts in Mice
This study suggests that progesterone treatment in neonatal mice does not cause any structural changes in the male reproductive tracts at puberty, unlike the neonatal estrogen treatment.PMID:38318101 | PMC:PMC10838630 | DOI:10.1267/ahc.23-00052 (Source: Acta Histochemica)
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Takuya Omotehara Hiroki Nakata Kenta Nagahori Miyuki Kuramasu Koichiro Ichimura Masahiro Itoh Source Type: research
Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload
Acta Histochem Cytochem. 2023 Dec 28;56(6):111-119. doi: 10.1267/ahc.23-00041. Epub 2023 Dec 20.ABSTRACTMyogenic cell differentiation is modulated by multiple regulatory factors, such as myogenin, p21, and cyclin D3 during myogenesis in vitro. It is also recognized that myogenin and p21 play important roles in regulating muscle satellite cell (SC) differentiation during overload-induced muscle hypertrophy in vivo. However, the expression patterns and functional role of cyclin D3 in the progress of muscle hypertrophy remain unclear. Thus, the present study investigated cyclin D3 expression in skeletal muscles during early-s...
Source: Acta Histochemica - February 6, 2024 Category: Biochemistry Authors: Minenori Ishido Source Type: research
