AT-rich interactive domain 1A (ARID1A) cannot be considered a morphological marker for prostate cancer progression: A pilot study
Acta Histochem. 2022 Jan 14;124(2):151847. doi: 10.1016/j.acthis.2022.151847. Online ahead of print.ABSTRACTProstate cancer (PCa) is one of the most common cancers worldwide but it presents many subtypes and patient heterogeneity. It is necessary to discriminate localised not aggressive PCa and metastatic cancer in order to better define the personalised treatment. The identification of an appropriate biomarker to combine with Gleason grading system, that is one of the most important prognostic factors in prostate cancer outcome, remains a major clinical issue. We have tested AT-rich interactive domain 1A (ARID1A) in prost...
Source: Acta Histochemica - January 17, 2022 Category: Biochemistry Authors: Giovanni Tossetta Sonia Fantone Rosaria Gesuita Rodolfo Montironi Daniela Marzioni Roberta Mazzucchelli Source Type: research

Corrigendum to "Protective action of ultrasound-guided intraparenchymal transplantation of BMSCs in adriamycin nephropathy rats through the RIPK3/MLKL and NLRP3 pathways" [Acta Histochem. 123 (2021) 151773]
Acta Histochem. 2022 Jan 12:151846. doi: 10.1016/j.acthis.2022.151846. Online ahead of print.NO ABSTRACTPMID:35033378 | DOI:10.1016/j.acthis.2022.151846 (Source: Acta Histochemica)
Source: Acta Histochemica - January 16, 2022 Category: Biochemistry Authors: Chunjuan Xia Lishi Shao Yiqun Ma Xinghong Wang Ya Zhang Cheng Shi Jiaqi Li Weihu Zhang Hongjun Li Jiaping Wang Source Type: research

Markers to sensibility and relapse on IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models cisplatin-treated
Acta Histochem. 2022 Jan 13;124(2):151849. doi: 10.1016/j.acthis.2022.151849. Online ahead of print.ABSTRACTThe complexity of different components of tumor stroma poses huge challenges for therapies targeting the neuroblastoma (NB) microenvironment. The present study aimed to evaluate platinum-based response in IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models. For this, we evaluated mRNA expression of heat shock proteins HSPA1A, HSPB1, TRAP1, HSPA1AL, HSPD1, and DNA damage repair gene ERCC1. After treatment, residual cells were grafted on CAM (chicken chorioallantoic membrane) to evalua...
Source: Acta Histochemica - January 16, 2022 Category: Biochemistry Authors: Bryan Ôrtero Perez Gonçalves Warne Pedro de Andrade S ílvia Ligório Fialho Luciana Maria Silva Source Type: research

Toxicopathological impact of sub-lethal concentrations of lead nitrate on the gill of the catfish Heteropneustes fossilis
Acta Histochem. 2022 Jan 13;124(2):151848. doi: 10.1016/j.acthis.2022.151848. Online ahead of print.ABSTRACTIn recent studies, fish are heavily used as biomarkers of aquatic pollution, and heavy metals are among the main contributors to water pollution. In the present study, we investigated histopathological changes along with alterations in localization and activity of enzymes alkaline phosphatase (ALP), acid phosphatase (ACP), catalase (CAT), peroxidase (PER) and Na+/K+-ATPase in the gill tissues of Indian stinging catfish Heteropneustes fossilis exposed to two different concentrations (0.4 and 4 mg/L) of lead nitrate fo...
Source: Acta Histochemica - January 16, 2022 Category: Biochemistry Authors: Archisman Mahapatra Arup Mistri Priya Gupta Sumanta Kar Swati Mittal Rahul K Singh Source Type: research

Corrigendum to "Protective action of ultrasound-guided intraparenchymal transplantation of BMSCs in adriamycin nephropathy rats through the RIPK3/MLKL and NLRP3 pathways" [Acta Histochem. 123 (2021) 151773]
Acta Histochem. 2022 Jan 12:151846. doi: 10.1016/j.acthis.2022.151846. Online ahead of print.NO ABSTRACTPMID:35033378 | DOI:10.1016/j.acthis.2022.151846 (Source: Acta Histochemica)
Source: Acta Histochemica - January 16, 2022 Category: Biochemistry Authors: Chunjuan Xia Lishi Shao Yiqun Ma Xinghong Wang Ya Zhang Cheng Shi Jiaqi Li Weihu Zhang Hongjun Li Jiaping Wang Source Type: research

Markers to sensibility and relapse on IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models cisplatin-treated
Acta Histochem. 2022 Jan 13;124(2):151849. doi: 10.1016/j.acthis.2022.151849. Online ahead of print.ABSTRACTThe complexity of different components of tumor stroma poses huge challenges for therapies targeting the neuroblastoma (NB) microenvironment. The present study aimed to evaluate platinum-based response in IMR-32 neuroblastoma cell line cultured in monolayer (2D) and neurosphere (3D) models. For this, we evaluated mRNA expression of heat shock proteins HSPA1A, HSPB1, TRAP1, HSPA1AL, HSPD1, and DNA damage repair gene ERCC1. After treatment, residual cells were grafted on CAM (chicken chorioallantoic membrane) to evalua...
Source: Acta Histochemica - January 16, 2022 Category: Biochemistry Authors: Bryan Ôrtero Perez Gonçalves Warne Pedro de Andrade S ílvia Ligório Fialho Luciana Maria Silva Source Type: research

Toxicopathological impact of sub-lethal concentrations of lead nitrate on the gill of the catfish Heteropneustes fossilis
Acta Histochem. 2022 Jan 13;124(2):151848. doi: 10.1016/j.acthis.2022.151848. Online ahead of print.ABSTRACTIn recent studies, fish are heavily used as biomarkers of aquatic pollution, and heavy metals are among the main contributors to water pollution. In the present study, we investigated histopathological changes along with alterations in localization and activity of enzymes alkaline phosphatase (ALP), acid phosphatase (ACP), catalase (CAT), peroxidase (PER) and Na+/K+-ATPase in the gill tissues of Indian stinging catfish Heteropneustes fossilis exposed to two different concentrations (0.4 and 4 mg/L) of lead nitrate fo...
Source: Acta Histochemica - January 16, 2022 Category: Biochemistry Authors: Archisman Mahapatra Arup Mistri Priya Gupta Sumanta Kar Swati Mittal Rahul K Singh Source Type: research

The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination
Acta Histochem Cytochem. 2021 Dec 24;54(6):177-183. doi: 10.1267/ahc.21-00084. Epub 2021 Nov 18.ABSTRACTThe therapeutic strategy is determined by protein expression using immunohistochemistry of estrogen receptor (ER), progesterone receptor, and human epidermal growth factor receptor 2 (HER2) in formalin-fixed paraffin-embedded (FFPE) breast cancer tissues. However, few proteins function independently, and many of them functions due to protein-protein interactions (PPIs) with other proteins. Therefore, it is important to focus on PPIs. This review summarizes the PPIs of ER and HER2 in breast cancer, especially those using ...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Erina Iwabuchi Yasuhiro Miki Hironobu Sasano Source Type: research

Differences in Regression Patterns of Complete and Incomplete Intestinal Metaplasia at Ten Years after < em > Helicobacter pylori < /em > Eradication
This study was conducted to reveal the reversibility of subtype of intestinal metaplasia (IM) and Paneth cells after H. pylori eradication (HPE). Among 75 patients, we retrospectively examined the proportions of patients with complete type of IM (CIM), incomplete type of IM (IIM) and Paneth cells in their biopsy specimens obtained from the greater curvature of the antrum (A2) and the greater curvature of the middle corpus (B2) before and during a follow-up period of 10 years after HPE. Immunohistochemistry was used to determine IM type. Compared to before HPE, the proportion of patients with CIM did not decrease significan...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Yasuhiro Wada Masaaki Kodama Kazuhiro Mizukami Tadayoshi Okimoto Takafumi Fuchino Koshiro Tsutsumi Masahide Fukuda Yuka Hirashita Kensuke Fukuda Kazuhisa Okamoto Ryo Ogawa Ryoji Kushima Kazunari Murakami Source Type: research

Nuclear Expression of Pygo2 Correlates with Poorly Differentiated State Involving c-Myc, PCNA and Bcl9 in Myanmar Hepatocellular Carcinoma
Acta Histochem Cytochem. 2021 Dec 24;54(6):195-206. doi: 10.1267/ahc.21-00090. Epub 2021 Dec 21.ABSTRACTIn Myanmar, hepatocellular carcinoma (HCC) is commonly seen in young adult and associated with poor prognosis, while the molecular mechanisms that characterize HCC in Myanmar are unknown. As co-activation of Wnt/β-catenin signaling and c-Myc (Myc) are reported to associate with malignancy of HCC, we immunohistochemically investigated the expression of Pygo2 and Bcl9, the co-activators of the Wnt/β-catenin signaling, Myc and PCNA in 60 cases of Myanmar HCC. Pygo2 expression was confirmed by in situ hybridization...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Myo Win Htun Yasuaki Shibata Kyaw Soe Takehiko Koji Source Type: research

The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination
Acta Histochem Cytochem. 2021 Dec 24;54(6):177-183. doi: 10.1267/ahc.21-00084. Epub 2021 Nov 18.ABSTRACTThe therapeutic strategy is determined by protein expression using immunohistochemistry of estrogen receptor (ER), progesterone receptor, and human epidermal growth factor receptor 2 (HER2) in formalin-fixed paraffin-embedded (FFPE) breast cancer tissues. However, few proteins function independently, and many of them functions due to protein-protein interactions (PPIs) with other proteins. Therefore, it is important to focus on PPIs. This review summarizes the PPIs of ER and HER2 in breast cancer, especially those using ...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Erina Iwabuchi Yasuhiro Miki Hironobu Sasano Source Type: research

Differences in Regression Patterns of Complete and Incomplete Intestinal Metaplasia at Ten Years after < em > Helicobacter pylori < /em > Eradication
This study was conducted to reveal the reversibility of subtype of intestinal metaplasia (IM) and Paneth cells after H. pylori eradication (HPE). Among 75 patients, we retrospectively examined the proportions of patients with complete type of IM (CIM), incomplete type of IM (IIM) and Paneth cells in their biopsy specimens obtained from the greater curvature of the antrum (A2) and the greater curvature of the middle corpus (B2) before and during a follow-up period of 10 years after HPE. Immunohistochemistry was used to determine IM type. Compared to before HPE, the proportion of patients with CIM did not decrease significan...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Yasuhiro Wada Masaaki Kodama Kazuhiro Mizukami Tadayoshi Okimoto Takafumi Fuchino Koshiro Tsutsumi Masahide Fukuda Yuka Hirashita Kensuke Fukuda Kazuhisa Okamoto Ryo Ogawa Ryoji Kushima Kazunari Murakami Source Type: research

Nuclear Expression of Pygo2 Correlates with Poorly Differentiated State Involving c-Myc, PCNA and Bcl9 in Myanmar Hepatocellular Carcinoma
Acta Histochem Cytochem. 2021 Dec 24;54(6):195-206. doi: 10.1267/ahc.21-00090. Epub 2021 Dec 21.ABSTRACTIn Myanmar, hepatocellular carcinoma (HCC) is commonly seen in young adult and associated with poor prognosis, while the molecular mechanisms that characterize HCC in Myanmar are unknown. As co-activation of Wnt/β-catenin signaling and c-Myc (Myc) are reported to associate with malignancy of HCC, we immunohistochemically investigated the expression of Pygo2 and Bcl9, the co-activators of the Wnt/β-catenin signaling, Myc and PCNA in 60 cases of Myanmar HCC. Pygo2 expression was confirmed by in situ hybridization...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Myo Win Htun Yasuaki Shibata Kyaw Soe Takehiko Koji Source Type: research

The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination
Acta Histochem Cytochem. 2021 Dec 24;54(6):177-183. doi: 10.1267/ahc.21-00084. Epub 2021 Nov 18.ABSTRACTThe therapeutic strategy is determined by protein expression using immunohistochemistry of estrogen receptor (ER), progesterone receptor, and human epidermal growth factor receptor 2 (HER2) in formalin-fixed paraffin-embedded (FFPE) breast cancer tissues. However, few proteins function independently, and many of them functions due to protein-protein interactions (PPIs) with other proteins. Therefore, it is important to focus on PPIs. This review summarizes the PPIs of ER and HER2 in breast cancer, especially those using ...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Erina Iwabuchi Yasuhiro Miki Hironobu Sasano Source Type: research

Differences in Regression Patterns of Complete and Incomplete Intestinal Metaplasia at Ten Years after < em > Helicobacter pylori < /em > Eradication
This study was conducted to reveal the reversibility of subtype of intestinal metaplasia (IM) and Paneth cells after H. pylori eradication (HPE). Among 75 patients, we retrospectively examined the proportions of patients with complete type of IM (CIM), incomplete type of IM (IIM) and Paneth cells in their biopsy specimens obtained from the greater curvature of the antrum (A2) and the greater curvature of the middle corpus (B2) before and during a follow-up period of 10 years after HPE. Immunohistochemistry was used to determine IM type. Compared to before HPE, the proportion of patients with CIM did not decrease significan...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Yasuhiro Wada Masaaki Kodama Kazuhiro Mizukami Tadayoshi Okimoto Takafumi Fuchino Koshiro Tsutsumi Masahide Fukuda Yuka Hirashita Kensuke Fukuda Kazuhisa Okamoto Ryo Ogawa Ryoji Kushima Kazunari Murakami Source Type: research

Nuclear Expression of Pygo2 Correlates with Poorly Differentiated State Involving c-Myc, PCNA and Bcl9 in Myanmar Hepatocellular Carcinoma
Acta Histochem Cytochem. 2021 Dec 24;54(6):195-206. doi: 10.1267/ahc.21-00090. Epub 2021 Dec 21.ABSTRACTIn Myanmar, hepatocellular carcinoma (HCC) is commonly seen in young adult and associated with poor prognosis, while the molecular mechanisms that characterize HCC in Myanmar are unknown. As co-activation of Wnt/β-catenin signaling and c-Myc (Myc) are reported to associate with malignancy of HCC, we immunohistochemically investigated the expression of Pygo2 and Bcl9, the co-activators of the Wnt/β-catenin signaling, Myc and PCNA in 60 cases of Myanmar HCC. Pygo2 expression was confirmed by in situ hybridization...
Source: Acta Histochemica - January 13, 2022 Category: Biochemistry Authors: Myo Win Htun Yasuaki Shibata Kyaw Soe Takehiko Koji Source Type: research

Exposure to aluminium chloride during the peripuberal period induces prostate damage in male rats
In conclusion, the exposure to aluminium chloride during the peripubertal period impairs the prostatic development.PMID:35021147 | DOI:10.1016/j.acthis.2022.151843 (Source: Acta Histochemica)
Source: Acta Histochemica - January 12, 2022 Category: Biochemistry Authors: Franciely A V D Leal Gessica D Gon çalves Jo ão Gabriel M Soncini Larissa Staurengo-Ferrari Victor Fattori Waldiceu A Verri Wellerson R Scarano Glaura S A Fernandes Source Type: research

Revisiting 40 years ago
Acta Histochem. 2022 Jan 7:151845. doi: 10.1016/j.acthis.2022.151845. Online ahead of print.NO ABSTRACTPMID:35012756 | DOI:10.1016/j.acthis.2022.151845 (Source: Acta Histochemica)
Source: Acta Histochemica - January 11, 2022 Category: Biochemistry Authors: Steven B Oppenheimer Source Type: research

Revisiting 40 years ago
Acta Histochem. 2022 Jan 7:151845. doi: 10.1016/j.acthis.2022.151845. Online ahead of print.NO ABSTRACTPMID:35012756 | DOI:10.1016/j.acthis.2022.151845 (Source: Acta Histochemica)
Source: Acta Histochemica - January 11, 2022 Category: Biochemistry Authors: Steven B Oppenheimer Source Type: research

The role of KLF4 in melanogenesis and homeostasis in sheep melanocytes
Acta Histochem. 2022 Jan 5;124(1):151839. doi: 10.1016/j.acthis.2021.151839. Online ahead of print.ABSTRACTKLF4 expression has been associated with hair color in mammals and has also been found to regulate melanoma cell growth. Here, we assessed the influence of KLF4 on coat color formation and melanocytes. We found that KLF4 was highly expressed in the black skin of sheep both at the mRNA and protein levels compared with white skin. KLF4 immunostaining further showed that KLF4 protein was mainly expressed in epidermal, outer root, and hair bulb regions. In sheep melanocytes, the proliferation of melanocytes was inhibited ...
Source: Acta Histochemica - January 8, 2022 Category: Biochemistry Authors: Hong Qin Yuxiao Chen Shuai Wang Shihao Ge Quanhai Pang Source Type: research

The role of KLF4 in melanogenesis and homeostasis in sheep melanocytes
Acta Histochem. 2022 Jan 5;124(1):151839. doi: 10.1016/j.acthis.2021.151839. Online ahead of print.ABSTRACTKLF4 expression has been associated with hair color in mammals and has also been found to regulate melanoma cell growth. Here, we assessed the influence of KLF4 on coat color formation and melanocytes. We found that KLF4 was highly expressed in the black skin of sheep both at the mRNA and protein levels compared with white skin. KLF4 immunostaining further showed that KLF4 protein was mainly expressed in epidermal, outer root, and hair bulb regions. In sheep melanocytes, the proliferation of melanocytes was inhibited ...
Source: Acta Histochemica - January 8, 2022 Category: Biochemistry Authors: Hong Qin Yuxiao Chen Shuai Wang Shihao Ge Quanhai Pang Source Type: research

The role of KLF4 in melanogenesis and homeostasis in sheep melanocytes
Acta Histochem. 2022 Jan 5;124(1):151839. doi: 10.1016/j.acthis.2021.151839. Online ahead of print.ABSTRACTKLF4 expression has been associated with hair color in mammals and has also been found to regulate melanoma cell growth. Here, we assessed the influence of KLF4 on coat color formation and melanocytes. We found that KLF4 was highly expressed in the black skin of sheep both at the mRNA and protein levels compared with white skin. KLF4 immunostaining further showed that KLF4 protein was mainly expressed in epidermal, outer root, and hair bulb regions. In sheep melanocytes, the proliferation of melanocytes was inhibited ...
Source: Acta Histochemica - January 8, 2022 Category: Biochemistry Authors: Hong Qin Yuxiao Chen Shuai Wang Shihao Ge Quanhai Pang Source Type: research

Dual VEGF/PDGF knockdown suppresses vasculogenic mimicry formation in choroidal melanoma cells via the Wnt5a/ β-catenin/AKT signaling pathway
CONCLUSIONS: Dual VEGF/PDGF knockdown suppressed cell growth and metastasis in OCM-1 cells, and blocked the Wnt5a/β-catenin/AKT signaling pathway thereby inhibiting VM formation.PMID:34995928 | DOI:10.1016/j.acthis.2021.151842 (Source: Acta Histochemica)
Source: Acta Histochemica - January 7, 2022 Category: Biochemistry Authors: Yingying Yuan Bochao Geng Xiaoyan Xu Han Zhao Jingyi Bai Zhizhi Dou Shaoyou Jia Xiaoling Yu Wenjuan Luo Source Type: research

A new insight into cell biological and biochemical changes through aging
Acta Histochem. 2022 Jan 4;124(1):151841. doi: 10.1016/j.acthis.2021.151841. Online ahead of print.ABSTRACTAfter several years of extensive research, the main cause of aging is yet elusive. There are some theories about aging, such as stem cell aging, senescent cells accumulation, and neuro-endocrine theories. None of them is able to explain all changes that happen in cells and body through aging. By finding out the main cause of aging, it will be much easier to control, prevent and even reverse the aging process. Our cells, regardless of their replicative capacity, get old through aging and they have almost the same epige...
Source: Acta Histochemica - January 7, 2022 Category: Biochemistry Authors: Somayeh Sahabi Davoud Jafari-Gharabaghlou Nosratollah Zarghami Source Type: research

Expression pattern of Ptch2 in mouse embryonic maxillofacial development
Acta Histochem. 2021 Dec 31;124(1):151835. doi: 10.1016/j.acthis.2021.151835. Online ahead of print.ABSTRACTEmbryogenesis is modulated by numerous complex signaling cascades, which are essential for normal development. The Hedgehog (Hh) signaling pathway is part of these central cascades. As a homolog of Patched (Ptch)-1, Ptch2 initially did not appear to be as important as Ptch1. Recent reports have revealed that Ptch2 plays a crucial role in ligand-dependent feedback inhibition of Hh signaling in vertebrates. The role of Ptch2 in facial development remains unclear. Here, we investigated the detailed expression pattern of...
Source: Acta Histochemica - January 3, 2022 Category: Biochemistry Authors: Xiaotong Wang Zhihong Ma Yan Wu Jing Chen Xia Peng Yijia Wang Ming Fan Juan Du Source Type: research

Formaldehyde-hardened albumin as a non-penetrating embedding matrix for frozen and vibratome sectioning
Acta Histochem. 2021 Dec 31;124(1):151838. doi: 10.1016/j.acthis.2021.151838. Online ahead of print.ABSTRACTIn this paper, we describe a protocol for a non-penetrating embedding matrix that can be used for frozen or vibratome sectioning of various formaldehyde-fixed tissue specimens. In our experiments, we wanted to prepare thin frozen sections from miniature specimens for fluorescent staining. As we could not achieve satisfactory results with any of the previously published methods, we have tried to modify the existing protocols, and systematically evaluated the effect of these modifications on the properties of the embed...
Source: Acta Histochemica - January 3, 2022 Category: Biochemistry Authors: Ivo Vanick ý Juraj Bla ško Jana Kon čeková Zuzana Dzurja šková Zuzana Michalov á Eva Sz ékiová Source Type: research

E3 ubiquitin-protein ligase 2 inhibits cell proliferation, migration, and invasion of non-small cell lung cancer through ubiquitination of Notch1
This study aimed to explore the role of MIB2 in non-small cell lung cancer (NSCLC) and the underlying mechanism. Quantitative real-time PCR (QRT-PCR) and western blot were first performed to detect MIB2 expression in tumor tissues obtained from NSCLC patients (n = 30) and NSCLC cells, respectively. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and transwell assays were then used to examine the effect of MIB2 on the proliferation, migration and invasion of NSCLC cells. Western blot was further performed to examine the effect of Mind bomb 2 (MIB2), an E3 ligase on Notch1 protein and its ubiquitination. M...
Source: Acta Histochemica - December 31, 2021 Category: Biochemistry Authors: Yuening Guo Xiaowen Chi Si'ying Qu Yu Sun Jing Liu Lijuan Zhang Caihong Cao Yansha Wang Wei Zhang Source Type: research

E3 ubiquitin-protein ligase 2 inhibits cell proliferation, migration, and invasion of non-small cell lung cancer through ubiquitination of Notch1
This study aimed to explore the role of MIB2 in non-small cell lung cancer (NSCLC) and the underlying mechanism. Quantitative real-time PCR (QRT-PCR) and western blot were first performed to detect MIB2 expression in tumor tissues obtained from NSCLC patients (n = 30) and NSCLC cells, respectively. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and transwell assays were then used to examine the effect of MIB2 on the proliferation, migration and invasion of NSCLC cells. Western blot was further performed to examine the effect of Mind bomb 2 (MIB2), an E3 ligase on Notch1 protein and its ubiquitination. M...
Source: Acta Histochemica - December 31, 2021 Category: Biochemistry Authors: Yuening Guo Xiaowen Chi Si'ying Qu Yu Sun Jing Liu Lijuan Zhang Caihong Cao Yansha Wang Wei Zhang Source Type: research

E3 ubiquitin-protein ligase 2 inhibits cell proliferation, migration, and invasion of non-small cell lung cancer through ubiquitination of Notch1
This study aimed to explore the role of MIB2 in non-small cell lung cancer (NSCLC) and the underlying mechanism. Quantitative real-time PCR (QRT-PCR) and western blot were first performed to detect MIB2 expression in tumor tissues obtained from NSCLC patients (n = 30) and NSCLC cells, respectively. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and transwell assays were then used to examine the effect of MIB2 on the proliferation, migration and invasion of NSCLC cells. Western blot was further performed to examine the effect of Mind bomb 2 (MIB2), an E3 ligase on Notch1 protein and its ubiquitination. M...
Source: Acta Histochemica - December 31, 2021 Category: Biochemistry Authors: Yuening Guo Xiaowen Chi Si'ying Qu Yu Sun Jing Liu Lijuan Zhang Caihong Cao Yansha Wang Wei Zhang Source Type: research

FGF2 and FGF10 expression patterns in the epidermis and mesenchyme during homeotic transformation of tail into hindlimbs in frog tadpoles
Acta Histochem. 2021 Dec 28;124(1):151836. doi: 10.1016/j.acthis.2021.151836. Online ahead of print.ABSTRACTLimbs are trunk quintessential in tetrapods. Their development relies on the Retinoic acid (RA) gradient in association with the Fibroblast Growth Factors (FGFs). The role of various FGFs have been probed extensively and confirmed during the induction of ectopic limbs in vertebrates. On such factual backdrops, we studied the expression patterns of FGF2 and FGF10 in the epidermis and mesenchyme by immunohistochemical localization in the regenerating tails of tadpoles of the Indian tree frog, Polypedates maculatus. The...
Source: Acta Histochemica - December 30, 2021 Category: Biochemistry Authors: Jutshina Hota Pravati Kumari Mahapatra Source Type: research

FGF2 and FGF10 expression patterns in the epidermis and mesenchyme during homeotic transformation of tail into hindlimbs in frog tadpoles
Acta Histochem. 2021 Dec 27;124(1):151836. doi: 10.1016/j.acthis.2021.151836. Online ahead of print.ABSTRACTLimbs are trunk quintessential in tetrapods. Their development relies on the Retinoic acid (RA) gradient in association with the Fibroblast Growth Factors (FGFs). The role of various FGFs have been probed extensively and confirmed during the induction of ectopic limbs in vertebrates. On such factual backdrops, we studied the expression patterns of FGF2 and FGF10 in the epidermis and mesenchyme by immunohistochemical localization in the regenerating tails of tadpoles of the Indian tree frog, Polypedates maculatus. The...
Source: Acta Histochemica - December 30, 2021 Category: Biochemistry Authors: Jutshina Hota Pravati Kumari Mahapatra Source Type: research

Drp1 knockdown represses apoptosis of rat retinal endothelial cells by inhibiting mitophagy
In conclusion, these data demonstrated that Drp1 knockdown repressed apoptosis of rat retinal endothelial cells by inhibiting mitophagy. Thus, this work suggests that targeted regulation of Drp1 may become a treatment for DR.PMID:34959219 | DOI:10.1016/j.acthis.2021.151837 (Source: Acta Histochemica)
Source: Acta Histochemica - December 27, 2021 Category: Biochemistry Authors: Hongxi Wu Guodong Li Weiwei Chen Wenbin Luo Zhongyi Yang Zhipeng You Yuling Zou Source Type: research

Drp1 knockdown represses apoptosis of rat retinal endothelial cells by inhibiting mitophagy
In conclusion, these data demonstrated that Drp1 knockdown repressed apoptosis of rat retinal endothelial cells by inhibiting mitophagy. Thus, this work suggests that targeted regulation of Drp1 may become a treatment for DR.PMID:34959219 | DOI:10.1016/j.acthis.2021.151837 (Source: Acta Histochemica)
Source: Acta Histochemica - December 27, 2021 Category: Biochemistry Authors: Hongxi Wu Guodong Li Weiwei Chen Wenbin Luo Zhongyi Yang Zhipeng You Yuling Zou Source Type: research

Drp1 knockdown represses apoptosis of rat retinal endothelial cells by inhibiting mitophagy
In conclusion, these data demonstrated that Drp1 knockdown repressed apoptosis of rat retinal endothelial cells by inhibiting mitophagy. Thus, this work suggests that targeted regulation of Drp1 may become a treatment for DR.PMID:34959219 | DOI:10.1016/j.acthis.2021.151837 (Source: Acta Histochemica)
Source: Acta Histochemica - December 27, 2021 Category: Biochemistry Authors: Hongxi Wu Guodong Li Weiwei Chen Wenbin Luo Zhongyi Yang Zhipeng You Yuling Zou Source Type: research

Enhanced carbonic anhydrase expression with calcification and fibrosis in bronchial cartilage during COPD
Acta Histochem. 2021 Dec 23;124(1):151834. doi: 10.1016/j.acthis.2021.151834. Online ahead of print.ABSTRACTPulmonary cartilage plays a crucial structural role determining the physiologic airway compressibility and distensibility, necessary for proper mechanical function. This functionality deteriorates with aging due to increased stiffness of both airway muscle and cartilage, as well as, decreased renewal capacity. Altered airway remodeling has been suggested as a pathogenic driver of chronic obstructive pulmonary disease (COPD) through mechanisms still incompletely understood. Using paraffin-embedded lung tissue sections...
Source: Acta Histochemica - December 26, 2021 Category: Biochemistry Authors: Victor E Nava Rahul Khosla Samuel Shin Federico E Mordini Bidhan C Bandyopadhyay Source Type: research

Enhanced carbonic anhydrase expression with calcification and fibrosis in bronchial cartilage during COPD
Acta Histochem. 2021 Dec 23;124(1):151834. doi: 10.1016/j.acthis.2021.151834. Online ahead of print.ABSTRACTPulmonary cartilage plays a crucial structural role determining the physiologic airway compressibility and distensibility, necessary for proper mechanical function. This functionality deteriorates with aging due to increased stiffness of both airway muscle and cartilage, as well as, decreased renewal capacity. Altered airway remodeling has been suggested as a pathogenic driver of chronic obstructive pulmonary disease (COPD) through mechanisms still incompletely understood. Using paraffin-embedded lung tissue sections...
Source: Acta Histochemica - December 26, 2021 Category: Biochemistry Authors: Victor E Nava Rahul Khosla Samuel Shin Federico E Mordini Bidhan C Bandyopadhyay Source Type: research

Effect of astaxanthin and melatonin on cell viability and DNA damage in human breast cancer cell lines
CONCLUSION: Furthermore, the presence of astaxanthin increased the function of melatonin-induced cell death in breast cancer cells.PMID:34952259 | DOI:10.1016/j.acthis.2021.151832 (Source: Acta Histochemica)
Source: Acta Histochemica - December 24, 2021 Category: Biochemistry Authors: Aida Karimian Fereshteh Mir Mohammadrezaei Akbar Hajizadeh Moghadam Mohammad Hadi Bahadori Marjan Ghorbani-Anarkooli Asadollah Asadi Arash Abdolmaleki Source Type: research

Effect of astaxanthin and melatonin on cell viability and DNA damage in human breast cancer cell lines
CONCLUSION: Furthermore, the presence of astaxanthin increased the function of melatonin-induced cell death in breast cancer cells.PMID:34952259 | DOI:10.1016/j.acthis.2021.151832 (Source: Acta Histochemica)
Source: Acta Histochemica - December 24, 2021 Category: Biochemistry Authors: Aida Karimian Fereshteh Mir Mohammadrezaei Akbar Hajizadeh Moghadam Mohammad Hadi Bahadori Marjan Ghorbani-Anarkooli Asadollah Asadi Arash Abdolmaleki Source Type: research

Paracrine mechanisms of endothelial progenitor cells in vascular repair
Acta Histochem. 2021 Dec 17;124(1):151833. doi: 10.1016/j.acthis.2021.151833. Online ahead of print.ABSTRACTEndothelial progenitor cells (EPCs) play an important role in repairing damaged blood vessels and promoting neovascularization. However, the specific mechanism of EPCs promoting vascular repair is still unclear. Currently, there are two different views on the repair of damaged vessels by EPCs, one is that EPCs can directly differentiate into endothelial cells (ECs) and integrate into injured vessels, the other is that EPCs act on cells and blood vessels by releasing paracrine substances. But more evidence now support...
Source: Acta Histochemica - December 20, 2021 Category: Biochemistry Authors: Fanchen Yan Xiaodan Liu Huang Ding Wei Zhang Source Type: research

Paracrine mechanisms of endothelial progenitor cells in vascular repair
Acta Histochem. 2021 Dec 17;124(1):151833. doi: 10.1016/j.acthis.2021.151833. Online ahead of print.ABSTRACTEndothelial progenitor cells (EPCs) play an important role in repairing damaged blood vessels and promoting neovascularization. However, the specific mechanism of EPCs promoting vascular repair is still unclear. Currently, there are two different views on the repair of damaged vessels by EPCs, one is that EPCs can directly differentiate into endothelial cells (ECs) and integrate into injured vessels, the other is that EPCs act on cells and blood vessels by releasing paracrine substances. But more evidence now support...
Source: Acta Histochemica - December 20, 2021 Category: Biochemistry Authors: Fanchen Yan Xiaodan Liu Huang Ding Wei Zhang Source Type: research

Paracrine mechanisms of endothelial progenitor cells in vascular repair
Acta Histochem. 2021 Dec 17;124(1):151833. doi: 10.1016/j.acthis.2021.151833. Online ahead of print.ABSTRACTEndothelial progenitor cells (EPCs) play an important role in repairing damaged blood vessels and promoting neovascularization. However, the specific mechanism of EPCs promoting vascular repair is still unclear. Currently, there are two different views on the repair of damaged vessels by EPCs, one is that EPCs can directly differentiate into endothelial cells (ECs) and integrate into injured vessels, the other is that EPCs act on cells and blood vessels by releasing paracrine substances. But more evidence now support...
Source: Acta Histochemica - December 20, 2021 Category: Biochemistry Authors: Fanchen Yan Xiaodan Liu Huang Ding Wei Zhang Source Type: research

Paracrine mechanisms of endothelial progenitor cells in vascular repair
Acta Histochem. 2021 Dec 17;124(1):151833. doi: 10.1016/j.acthis.2021.151833. Online ahead of print.ABSTRACTEndothelial progenitor cells (EPCs) play an important role in repairing damaged blood vessels and promoting neovascularization. However, the specific mechanism of EPCs promoting vascular repair is still unclear. Currently, there are two different views on the repair of damaged vessels by EPCs, one is that EPCs can directly differentiate into endothelial cells (ECs) and integrate into injured vessels, the other is that EPCs act on cells and blood vessels by releasing paracrine substances. But more evidence now support...
Source: Acta Histochemica - December 20, 2021 Category: Biochemistry Authors: Fanchen Yan Xiaodan Liu Huang Ding Wei Zhang Source Type: research

Guanylin ligand protects the intestinal immune barrier by activating the guanylate cyclase-C signaling pathway
Acta Histochem. 2021 Dec 15;124(1):151811. doi: 10.1016/j.acthis.2021.151811. Online ahead of print.ABSTRACTInflammatory bowel disease (IBD) impacts patient quality of life significantly. The dysfunction of intestinal immune barrier is closely associated with IBD. The guanylate cyclase-C (GC-C) signaling pathway activated by the guanylin (Gn) ligand is involved in the occurrence and development of IBD. However, how it regulates the intestinal immune barrier is still unclear. To investigate the effect of the GC-C pathway on intestinal mucosal immunity and provide experimental basis for seeking new therapeutic strategies for...
Source: Acta Histochemica - December 17, 2021 Category: Biochemistry Authors: Chengfeng Xing Tao Zhang Xiaolin Liu Canmei Li Gang Yang Hairong Zhang Rui Wang Kunhua Wang Yinglei Miao Danfeng Lan Source Type: research

Guanylin ligand protects the intestinal immune barrier by activating the guanylate cyclase-C signaling pathway
Acta Histochem. 2021 Dec 14;124(1):151811. doi: 10.1016/j.acthis.2021.151811. Online ahead of print.ABSTRACTInflammatory bowel disease (IBD) impacts patient quality of life significantly. The dysfunction of intestinal immune barrier is closely associated with IBD. The guanylate cyclase-C (GC-C) signaling pathway activated by the guanylin (Gn) ligand is involved in the occurrence and development of IBD. However, how it regulates the intestinal immune barrier is still unclear. To investigate the effect of the GC-C pathway on intestinal mucosal immunity and provide experimental basis for seeking new therapeutic strategies for...
Source: Acta Histochemica - December 17, 2021 Category: Biochemistry Authors: Chengfeng Xing Tao Zhang Xiaolin Liu Canmei Li Gang Yang Hairong Zhang Rui Wang Kunhua Wang Yinglei Miao Danfeng Lan Source Type: research

Guanylin ligand protects the intestinal immune barrier by activating the guanylate cyclase-C signaling pathway
Acta Histochem. 2021 Dec 14;124(1):151811. doi: 10.1016/j.acthis.2021.151811. Online ahead of print.ABSTRACTInflammatory bowel disease (IBD) impacts patient quality of life significantly. The dysfunction of intestinal immune barrier is closely associated with IBD. The guanylate cyclase-C (GC-C) signaling pathway activated by the guanylin (Gn) ligand is involved in the occurrence and development of IBD. However, how it regulates the intestinal immune barrier is still unclear. To investigate the effect of the GC-C pathway on intestinal mucosal immunity and provide experimental basis for seeking new therapeutic strategies for...
Source: Acta Histochemica - December 17, 2021 Category: Biochemistry Authors: Chengfeng Xing Tao Zhang Xiaolin Liu Canmei Li Gang Yang Hairong Zhang Rui Wang Kunhua Wang Yinglei Miao Danfeng Lan Source Type: research

L-Methionine prevents β-cell damage by modulating the expression of Arx, MafA and regulation of FOXO1 in type 1 diabetic rats
In conclusion, we report the involvement of L-Met in the modulation of α-cell identity marker (Arx), β-cell identity marker (MafA) and regulation of FOXO1 by histone methylation marks for the first time. We are of the opinion that this employed as a novel therapeutic approach for mitigating diabetes-induced β-cell death.PMID:34871948 | DOI:10.1016/j.acthis.2021.151820 (Source: Acta Histochemica)
Source: Acta Histochemica - December 6, 2021 Category: Biochemistry Authors: Umashanker Navik Kajal Rawat Kulbhushan Tikoo Source Type: research

L-Methionine prevents β-cell damage by modulating the expression of Arx, MafA and regulation of FOXO1 in type 1 diabetic rats
In conclusion, we report the involvement of L-Met in the modulation of α-cell identity marker (Arx), β-cell identity marker (MafA) and regulation of FOXO1 by histone methylation marks for the first time. We are of the opinion that this employed as a novel therapeutic approach for mitigating diabetes-induced β-cell death.PMID:34871948 | DOI:10.1016/j.acthis.2021.151820 (Source: Acta Histochemica)
Source: Acta Histochemica - December 6, 2021 Category: Biochemistry Authors: Umashanker Navik Kajal Rawat Kulbhushan Tikoo Source Type: research

L-Methionine prevents β-cell damage by modulating the expression of Arx, MafA and regulation of FOXO1 in type 1 diabetic rats
In conclusion, we report the involvement of L-Met in the modulation of α-cell identity marker (Arx), β-cell identity marker (MafA) and regulation of FOXO1 by histone methylation marks for the first time. We are of the opinion that this employed as a novel therapeutic approach for mitigating diabetes-induced β-cell death.PMID:34871948 | DOI:10.1016/j.acthis.2021.151820 (Source: Acta Histochemica)
Source: Acta Histochemica - December 6, 2021 Category: Biochemistry Authors: Umashanker Navik Kajal Rawat Kulbhushan Tikoo Source Type: research

L-Methionine prevents β-cell damage by modulating the expression of Arx, MafA and regulation of FOXO1 in type 1 diabetic rats
In conclusion, we report the involvement of L-Met in the modulation of α-cell identity marker (Arx), β-cell identity marker (MafA) and regulation of FOXO1 by histone methylation marks for the first time. We are of the opinion that this employed as a novel therapeutic approach for mitigating diabetes-induced β-cell death.PMID:34871948 | DOI:10.1016/j.acthis.2021.151820 (Source: Acta Histochemica)
Source: Acta Histochemica - December 6, 2021 Category: Biochemistry Authors: Umashanker Navik Kajal Rawat Kulbhushan Tikoo Source Type: research

L-Methionine prevents β-cell damage by modulating the expression of Arx, MafA and regulation of FOXO1 in type 1 diabetic rats
In conclusion, we report the involvement of L-Met in the modulation of α-cell identity marker (Arx), β-cell identity marker (MafA) and regulation of FOXO1 by histone methylation marks for the first time. We are of the opinion that this employed as a novel therapeutic approach for mitigating diabetes-induced β-cell death.PMID:34871948 | DOI:10.1016/j.acthis.2021.151820 (Source: Acta Histochemica)
Source: Acta Histochemica - December 6, 2021 Category: Biochemistry Authors: Umashanker Navik Kajal Rawat Kulbhushan Tikoo Source Type: research