The involvement of translationally controlled tumor protein during lamb rumen epithelium development
Acta Histochem. 2021 Jun 8;123(5):151737. doi: 10.1016/j.acthis.2021.151737. Online ahead of print.ABSTRACTEarly weaning is usually applied to improve the reproductive efficiency of sheep in mutton production, while the development of rumen is of vital importance for sheep weaning age. Translationally controlled tumor protein (TCTP) is a highly conserved protein which participates in multiple tissue and organ development. Thus, we hypothesized that TCTP was involved in sheep rumen development. Histological analyses of sheep rumen epithelium showed that the epithelium formed tough shaped papillae without growing from birth ...
Source: Acta Histochemica - June 11, 2021 Category: Biochemistry Authors: Kaizhi Zheng Jianliang Wu Liangyong Guo Yingyu Ying Peng Li Yang Cao Junfang Jiang Xin Huang Chunhua Meng Yongqing Jiang Source Type: research

The involvement of translationally controlled tumor protein during lamb rumen epithelium development
Acta Histochem. 2021 Jun 8;123(5):151737. doi: 10.1016/j.acthis.2021.151737. Online ahead of print.ABSTRACTEarly weaning is usually applied to improve the reproductive efficiency of sheep in mutton production, while the development of rumen is of vital importance for sheep weaning age. Translationally controlled tumor protein (TCTP) is a highly conserved protein which participates in multiple tissue and organ development. Thus, we hypothesized that TCTP was involved in sheep rumen development. Histological analyses of sheep rumen epithelium showed that the epithelium formed tough shaped papillae without growing from birth ...
Source: Acta Histochemica - June 11, 2021 Category: Biochemistry Authors: Kaizhi Zheng Jianliang Wu Liangyong Guo Yingyu Ying Peng Li Yang Cao Junfang Jiang Xin Huang Chunhua Meng Yongqing Jiang Source Type: research

Expression of renal vitamin D receptors and metabolizing enzymes in IgA nephropathy
Acta Histochem. 2021 Jun 7;123(5):151740. doi: 10.1016/j.acthis.2021.151740. Online ahead of print.ABSTRACTAIM: One of the main causes of end-stage renal disease (ESRD) in the world is IgA nephropathy (IgAN). Since kidney is a key player in vitamin D metabolism, we investigated the expression of renal vitamin D receptors (VDR) and metabolizing enzymes in IgA nephropathy patients (IgAN-P).METHODS: The sample included twelve IgAN-P who underwent ultrasound-guided renal biopsies and five controls who underwent nephrectomy due to clear renal carcinoma. Immunofluorescent staining was used to determine the expression of VDR, 25-...
Source: Acta Histochemica - June 10, 2021 Category: Biochemistry Authors: Adela Arapovi ć Katarina Vukojevi ć Merica Glavina Durdov Benjamin Benzon Ivana Šolić Anita Racetin Marija Juri ć Tanja Čujić Ivona Kosovi ć Mirela Lozi ć Dijana Bori ć Škaro Dragan Ljuti ć Mirna Saraga-Babi ć Ana Simi čić Majce Marijan Sar Source Type: research

Expression of renal vitamin D receptors and metabolizing enzymes in IgA nephropathy
Acta Histochem. 2021 Jun 7;123(5):151740. doi: 10.1016/j.acthis.2021.151740. Online ahead of print.ABSTRACTAIM: One of the main causes of end-stage renal disease (ESRD) in the world is IgA nephropathy (IgAN). Since kidney is a key player in vitamin D metabolism, we investigated the expression of renal vitamin D receptors (VDR) and metabolizing enzymes in IgA nephropathy patients (IgAN-P).METHODS: The sample included twelve IgAN-P who underwent ultrasound-guided renal biopsies and five controls who underwent nephrectomy due to clear renal carcinoma. Immunofluorescent staining was used to determine the expression of VDR, 25-...
Source: Acta Histochemica - June 10, 2021 Category: Biochemistry Authors: Adela Arapovi ć Katarina Vukojevi ć Merica Glavina Durdov Benjamin Benzon Ivana Šolić Anita Racetin Marija Juri ć Tanja Čujić Ivona Kosovi ć Mirela Lozi ć Dijana Bori ć Škaro Dragan Ljuti ć Mirna Saraga-Babi ć Ana Simi čić Majce Marijan Sar Source Type: research

Resveratrol activates PI3K/AKT to reduce myocardial cell apoptosis and mitochondrial oxidative damage caused by myocardial ischemia/reperfusion injury
Acta Histochem. 2021 Jun 6;123(5):151739. doi: 10.1016/j.acthis.2021.151739. Online ahead of print.ABSTRACTResveratrol is a kind of iPolyphenols widely existing in herbal medicine. Here we aim to investigate whether resveratrol can reduce the degree of myocardial ischemia/reperfusion (IR) injury and inhibit the development of oxidative stress, and elucidate the molecular mechanism of resveratrol in protecting myocardial cells. The primary rat cardiomyocytes were used to establish an ischemia/reperfusion model in vitro, and a series of routine biochemical experiments were conducted to explore the antioxidant and anti-apopto...
Source: Acta Histochemica - June 9, 2021 Category: Biochemistry Authors: Dongmin Yu Jianxian Xiong Yizhou Gao Jinghang Li Dawei Zhu Xiaowei Shen Lifu Sun Xiaowei Wang Source Type: research

Resveratrol activates PI3K/AKT to reduce myocardial cell apoptosis and mitochondrial oxidative damage caused by myocardial ischemia/reperfusion injury
Acta Histochem. 2021 Jun 6;123(5):151739. doi: 10.1016/j.acthis.2021.151739. Online ahead of print.ABSTRACTResveratrol is a kind of iPolyphenols widely existing in herbal medicine. Here we aim to investigate whether resveratrol can reduce the degree of myocardial ischemia/reperfusion (IR) injury and inhibit the development of oxidative stress, and elucidate the molecular mechanism of resveratrol in protecting myocardial cells. The primary rat cardiomyocytes were used to establish an ischemia/reperfusion model in vitro, and a series of routine biochemical experiments were conducted to explore the antioxidant and anti-apopto...
Source: Acta Histochemica - June 9, 2021 Category: Biochemistry Authors: Dongmin Yu Jianxian Xiong Yizhou Gao Jinghang Li Dawei Zhu Xiaowei Shen Lifu Sun Xiaowei Wang Source Type: research

Replacement of mitochondrion-rich cells during regeneration of the gills and opercular epithelium in zebrafish (Danio rerio)
We report regeneration of the operculum and proliferation of MRCs in regenerating tissue in adult zebrafish. These studies may contribute to our understanding of how MRC populations are regulated during the regenerative process, which may occur following exposure to environmental stressors, chemical toxicity or disease.PMID:34091038 | DOI:10.1016/j.acthis.2021.151738 (Source: Acta Histochemica)
Source: Acta Histochemica - June 6, 2021 Category: Biochemistry Authors: Frederic Nguyen Michael G Jonz Source Type: research

Replacement of mitochondrion-rich cells during regeneration of the gills and opercular epithelium in zebrafish (Danio rerio)
We report regeneration of the operculum and proliferation of MRCs in regenerating tissue in adult zebrafish. These studies may contribute to our understanding of how MRC populations are regulated during the regenerative process, which may occur following exposure to environmental stressors, chemical toxicity or disease.PMID:34091038 | DOI:10.1016/j.acthis.2021.151738 (Source: Acta Histochemica)
Source: Acta Histochemica - June 6, 2021 Category: Biochemistry Authors: Frederic Nguyen Michael G Jonz Source Type: research

Replacement of mitochondrion-rich cells during regeneration of the gills and opercular epithelium in zebrafish (Danio rerio)
We report regeneration of the operculum and proliferation of MRCs in regenerating tissue in adult zebrafish. These studies may contribute to our understanding of how MRC populations are regulated during the regenerative process, which may occur following exposure to environmental stressors, chemical toxicity or disease.PMID:34091038 | DOI:10.1016/j.acthis.2021.151738 (Source: Acta Histochemica)
Source: Acta Histochemica - June 6, 2021 Category: Biochemistry Authors: Frederic Nguyen Michael G Jonz Source Type: research

Spinal cord injury repair using mesenchymal stem cells derived from bone marrow in mice: A stereological study
Acta Histochem. 2021 May 31:151720. doi: 10.1016/j.acthis.2021.151720. Online ahead of print.ABSTRACTTransplantation of bone marrow stem cells (BMSCs) has shown to have a vital role in promoting nerve regeneration after SCI. The aim of this study was to investigate the effect of BMSCs transplantation in healing of spinal cord injury (SCI) in mice based on morphologic parameters. Forty two male mice were randomly divided into 3 groups of control with no intervention, experimental SCI without treatment, and experimental SCI transplanted with 2 × 105 BMSCs intravenously. To induce SCI bilaterally, T10 was compressed for...
Source: Acta Histochemica - June 4, 2021 Category: Biochemistry Authors: Zahra Khodabandeh Davood Mehrabani Farzaneh Dehghani Nooshin Gashmardi Mahboobeh Erfanizadeh Shahrokh Zare Farzaneh Bozorg-Ghalati Source Type: research

Spinal cord injury repair using mesenchymal stem cells derived from bone marrow in mice: A stereological study
Acta Histochem. 2021 May 31:151720. doi: 10.1016/j.acthis.2021.151720. Online ahead of print.ABSTRACTTransplantation of bone marrow stem cells (BMSCs) has shown to have a vital role in promoting nerve regeneration after SCI. The aim of this study was to investigate the effect of BMSCs transplantation in healing of spinal cord injury (SCI) in mice based on morphologic parameters. Forty two male mice were randomly divided into 3 groups of control with no intervention, experimental SCI without treatment, and experimental SCI transplanted with 2 × 105 BMSCs intravenously. To induce SCI bilaterally, T10 was compressed for...
Source: Acta Histochemica - June 4, 2021 Category: Biochemistry Authors: Zahra Khodabandeh Davood Mehrabani Farzaneh Dehghani Nooshin Gashmardi Mahboobeh Erfanizadeh Shahrokh Zare Farzaneh Bozorg-Ghalati Source Type: research

Lappaconitine hydrochloride inhibits proliferation and induces apoptosis in human colon cancer HCT-116 cells via mitochondrial and MAPK pathway
In conclusion, LH could induce apoptosis in HCT-116 cells through mitochondrial and MAPK signaling pathways. LH may be a promising treatment for CRC.PMID:34058516 | DOI:10.1016/j.acthis.2021.151736 (Source: Acta Histochemica)
Source: Acta Histochemica - May 31, 2021 Category: Biochemistry Authors: Na Song Junyi Ma Wei Hu Yongyue Guo Ling Hui Mohamed Aamer Jun Ma Source Type: research

Lappaconitine hydrochloride inhibits proliferation and induces apoptosis in human colon cancer HCT-116 cells via mitochondrial and MAPK pathway
In conclusion, LH could induce apoptosis in HCT-116 cells through mitochondrial and MAPK signaling pathways. LH may be a promising treatment for CRC.PMID:34058516 | DOI:10.1016/j.acthis.2021.151736 (Source: Acta Histochemica)
Source: Acta Histochemica - May 31, 2021 Category: Biochemistry Authors: Na Song Junyi Ma Wei Hu Yongyue Guo Ling Hui Mohamed Aamer Jun Ma Source Type: research

Lappaconitine hydrochloride inhibits proliferation and induces apoptosis in human colon cancer HCT-116 cells via mitochondrial and MAPK pathway
In conclusion, LH could induce apoptosis in HCT-116 cells through mitochondrial and MAPK signaling pathways. LH may be a promising treatment for CRC.PMID:34058516 | DOI:10.1016/j.acthis.2021.151736 (Source: Acta Histochemica)
Source: Acta Histochemica - May 31, 2021 Category: Biochemistry Authors: Na Song Junyi Ma Wei Hu Yongyue Guo Ling Hui Mohamed Aamer Jun Ma Source Type: research

Lappaconitine hydrochloride inhibits proliferation and induces apoptosis in human colon cancer HCT-116 cells via mitochondrial and MAPK pathway
In conclusion, LH could induce apoptosis in HCT-116 cells through mitochondrial and MAPK signaling pathways. LH may be a promising treatment for CRC.PMID:34058516 | DOI:10.1016/j.acthis.2021.151736 (Source: Acta Histochemica)
Source: Acta Histochemica - May 31, 2021 Category: Biochemistry Authors: Na Song Junyi Ma Wei Hu Yongyue Guo Ling Hui Mohamed Aamer Jun Ma Source Type: research

Inhibitory effect of Salvia coccinea on inflammatory responses through NF- κB signaling pathways in THP-1 cells and acute rat diabetes mellitus
This study was designed to evaluate the antioxidant effect of Aqueous Extract of S.coccinea leaf (AESL) in HG treated THP-1 cells and streptozotocin (STZ)-induced diabetic Wistar rats. AESL and the standard antidiabetic drug glibenclamide were administered orally by intragastric tube for 14 days and pre-treated HG grown THP-1 cells. AESL treatment reduced HG induced increase in ROS production, NF-κB dependent proinflammatory gene expression by influencing NF-κB nuclear translocation in THP-1 cells. Oral administration of AESL inhibited STZ-induced increase in serum lipid peroxidation, aspartate transaminase, al...
Source: Acta Histochemica - May 30, 2021 Category: Biochemistry Authors: Arun Sudaramoorthy Gobinath Shanmugam Narkunaraja Shanmugam Source Type: research

Ultrastructural aberrations, histological disruption and upregulation of the VEGF, CD34 and ASMA immunoexpression in the myocardium of anemic albino rats
Acta Histochem. 2021 May 27;123(5):151731. doi: 10.1016/j.acthis.2021.151731. Online ahead of print.ABSTRACTIron deficiency anemia (IDA) is a global health problem affecting various body systems and tissues including the cardiovascular system. Several literatures described the associated physiological and clinical changes in the cardiovascular system and heart. However, the associated structural changes were poorly investigated. Therefore, the main aim of the present work was to elucidate whether IDA induces structural changes and alterations in the VEGF, CD34 and ASMA immunoexpression in the myocardium of albino rats. Thi...
Source: Acta Histochemica - May 30, 2021 Category: Biochemistry Authors: Eyad M T Ali Sayed M El-Sayed Yasser M Elbastawisy Source Type: research

RN181 regulates the biological behaviors of oral squamous cell carcinoma cells via mediating ERK/MAPK signaling pathway
CONCLUSION: RN181 was down-regulated in OSCC, and it could inhibit the proliferation, invasion and migration, cause the G0/G1 arrest, while promote the apoptosis of OSCC cells via inhibiting ERK/MAPK pathway.PMID:34052676 | DOI:10.1016/j.acthis.2021.151733 (Source: Acta Histochemica)
Source: Acta Histochemica - May 30, 2021 Category: Biochemistry Authors: Ya Li Zhao-Yan Xiang Jie Xiong Zhou-Wen Hou Zhu Zhu Wei-Wei Bao Source Type: research

Inhibitory effect of Salvia coccinea on inflammatory responses through NF- κB signaling pathways in THP-1 cells and acute rat diabetes mellitus
This study was designed to evaluate the antioxidant effect of Aqueous Extract of S.coccinea leaf (AESL) in HG treated THP-1 cells and streptozotocin (STZ)-induced diabetic Wistar rats. AESL and the standard antidiabetic drug glibenclamide were administered orally by intragastric tube for 14 days and pre-treated HG grown THP-1 cells. AESL treatment reduced HG induced increase in ROS production, NF-κB dependent proinflammatory gene expression by influencing NF-κB nuclear translocation in THP-1 cells. Oral administration of AESL inhibited STZ-induced increase in serum lipid peroxidation, aspartate transaminase, al...
Source: Acta Histochemica - May 30, 2021 Category: Biochemistry Authors: Arun Sudaramoorthy Gobinath Shanmugam Narkunaraja Shanmugam Source Type: research

Ultrastructural aberrations, histological disruption and upregulation of the VEGF, CD34 and ASMA immunoexpression in the myocardium of anemic albino rats
Acta Histochem. 2021 May 27;123(5):151731. doi: 10.1016/j.acthis.2021.151731. Online ahead of print.ABSTRACTIron deficiency anemia (IDA) is a global health problem affecting various body systems and tissues including the cardiovascular system. Several literatures described the associated physiological and clinical changes in the cardiovascular system and heart. However, the associated structural changes were poorly investigated. Therefore, the main aim of the present work was to elucidate whether IDA induces structural changes and alterations in the VEGF, CD34 and ASMA immunoexpression in the myocardium of albino rats. Thi...
Source: Acta Histochemica - May 30, 2021 Category: Biochemistry Authors: Eyad M T Ali Sayed M El-Sayed Yasser M Elbastawisy Source Type: research

RN181 regulates the biological behaviors of oral squamous cell carcinoma cells via mediating ERK/MAPK signaling pathway
CONCLUSION: RN181 was down-regulated in OSCC, and it could inhibit the proliferation, invasion and migration, cause the G0/G1 arrest, while promote the apoptosis of OSCC cells via inhibiting ERK/MAPK pathway.PMID:34052676 | DOI:10.1016/j.acthis.2021.151733 (Source: Acta Histochemica)
Source: Acta Histochemica - May 30, 2021 Category: Biochemistry Authors: Ya Li Zhao-Yan Xiang Jie Xiong Zhou-Wen Hou Zhu Zhu Wei-Wei Bao Source Type: research

Dexmedetomidine reduces the apoptosis of rat hippocampal neurons via mediating ERK1/2 signal pathway by targeting miR-155
Acta Histochem. 2021 May 25;123(5):151734. doi: 10.1016/j.acthis.2021.151734. Online ahead of print.ABSTRACTRat hippocampal neurons were isolated and divided into Normal, oxygen glucose deprivation/reoxygenation (OGD/R), OGD/R + DEX, OGD/R + NC mimic, OGD/R + miR-155 mimic and OGD/R + DEX + miR-155 mimic groups. In OGD/R group, LDH, ROS and MDA levels and apoptosis rate was increased, with up-regulations of miR-155, Cyt c and Bax/Bcl-2 ratio, but decreases of SOD, GSH-Px and MMP levels, as well as down-regulations of p-ERK1/2/ERK1/2. As compared to the OGD/R group, parameters above in the OGD/R + DEX group were ameliorated...
Source: Acta Histochemica - May 28, 2021 Category: Biochemistry Authors: Yun-Sheng Zhu Zhen Liu Jia Min Ying-Fen Xiong Source Type: research

Propofol suppressed cell proliferation and enhanced apoptosis of bladder cancer cells by regulating the miR-340/CDK2 signal axis
CONCLUSION: In total, our results proved that targeting miR340/CDK2 axis was novel to enhance the anti-tumor effects of propofol in bladder cancer in vitro, and our study provided alternative therapeutic strategies for clinical treatment of bladder cancer.PMID:34048990 | DOI:10.1016/j.acthis.2021.151728 (Source: Acta Histochemica)
Source: Acta Histochemica - May 28, 2021 Category: Biochemistry Authors: Su-Hong Tan Hui-Juan Ding Xi-Ping Mei Ji-Tong Liu Yi-Xun Tang Yuan Li Source Type: research

Dexmedetomidine reduces the apoptosis of rat hippocampal neurons via mediating ERK1/2 signal pathway by targeting miR-155
Acta Histochem. 2021 May 25;123(5):151734. doi: 10.1016/j.acthis.2021.151734. Online ahead of print.ABSTRACTRat hippocampal neurons were isolated and divided into Normal, oxygen glucose deprivation/reoxygenation (OGD/R), OGD/R + DEX, OGD/R + NC mimic, OGD/R + miR-155 mimic and OGD/R + DEX + miR-155 mimic groups. In OGD/R group, LDH, ROS and MDA levels and apoptosis rate was increased, with up-regulations of miR-155, Cyt c and Bax/Bcl-2 ratio, but decreases of SOD, GSH-Px and MMP levels, as well as down-regulations of p-ERK1/2/ERK1/2. As compared to the OGD/R group, parameters above in the OGD/R + DEX group were ameliorated...
Source: Acta Histochemica - May 28, 2021 Category: Biochemistry Authors: Yun-Sheng Zhu Zhen Liu Jia Min Ying-Fen Xiong Source Type: research

Propofol suppressed cell proliferation and enhanced apoptosis of bladder cancer cells by regulating the miR-340/CDK2 signal axis
CONCLUSION: In total, our results proved that targeting miR340/CDK2 axis was novel to enhance the anti-tumor effects of propofol in bladder cancer in vitro, and our study provided alternative therapeutic strategies for clinical treatment of bladder cancer.PMID:34048990 | DOI:10.1016/j.acthis.2021.151728 (Source: Acta Histochemica)
Source: Acta Histochemica - May 28, 2021 Category: Biochemistry Authors: Su-Hong Tan Hui-Juan Ding Xi-Ping Mei Ji-Tong Liu Yi-Xun Tang Yuan Li Source Type: research

What Cyto- and Histochemistry Can Do to Crack the Sugar Code
Acta Histochem Cytochem. 2021 Apr 28;54(2):31-48. doi: 10.1267/ahc.21-00017. Epub 2021 Apr 17.ABSTRACTAs letters form the vocabulary of a language, biochemical 'symbols' (the building blocks of oligo- and polymers) make writing molecular messages possible. Compared to nucleotides and amino acids, sugars have chemical properties that facilitate to reach an unsurpassed level of oligomer diversity. These glycans are a part of the ubiquitous cellular glycoconjugates. Cyto- and histochemically, the glycans' structural complexity is mapped by glycophenotyping of cells and tissues using receptors ('readers', thus called lectins),...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Felix A Habermann Herbert Kaltner Alonso M Higuero Gabriel Garca Caballero Anna-Kristin Ludwig Joachim C Manning Jos Abad-Rodrguez Hans-Joachim Gabius Source Type: research

Development of Pathological Diagnosis Support System Using Micro-computed Tomography
In this study, we developed a system that supports the determination of the cutting position by visualizing and analyzing the internal structure of pathological material using micro-computed tomography (CT) before cutting. This system consists of a dedicated micro-CT and cutting support software. The micro-CT system has a fixture for fixing the target, enabling the scanning of easily deformable pathological materials. In the cutting support software, a function that interactively selects the extraction plane while displaying the volume rendering image and outputs a pseudo-histological image was implemented. Our results con...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Tomonari Hayakawa Atsushi Teramoto Yuka Kiriyama Tetsuya Tsukamoto Ayumi Yamada Kuniaki Saito Hiroshi Fujita Source Type: research

Altered Immunohistochemical Expression Patterns of HLA Class I during the Clinical Course of Cervical Intraepithelial Neoplasia
In conclusion, the immunohistochemical pattern of HLA class I expression is associated with the CIN grade, and it is alterable during the clinical course, especially in CIN2.PMID:34012177 | PMC:PMC8116618 | DOI:10.1267/ahc.21-00010 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Okuto Koguchi Haruna Nishimaki Yoko Nakanishi Hiroko Kobayashi Sumie Ohni Xiaoyan Tang Yoshiaki Kusumi Shinobu Masuda Source Type: research

Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts
Acta Histochem Cytochem. 2021 Apr 28;54(2):65-72. doi: 10.1267/ahc.21-00016. Epub 2021 Apr 17.ABSTRACTSpontaneous Raman spectroscopy, which senses changes in cellular contents of reduced cytochrome c, could be a powerful tool for label-free evaluation of ischemic hearts. However, undetermined is whether it is applicable to evaluation of myocardial viability in ischemic hearts. To address this issue, we investigated sequential changes in Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart before and during ligation of the left coronary artery and its subsequent release and re-ligation. Under ...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Koki Ikemoto Kosuke Hashimoto Yoshinori Harada Yasuaki Kumamoto Michiyo Hayakawa Kentaro Mochizuki Kazuhiko Matsuo Kenta Yashiro Hitoshi Yaku Tetsuro Takamatsu Hideo Tanaka Source Type: research

Supplementation with Fermented Barley Extract Prevents Mammary Epithelial Cell Invasion in an Early Breast Cancer Model
This study utilized the model system and investigated the effect of fermented barley extract (FBE), a food material. The elastic fiber layer is the outermost layer of the mammary duct. A reduction in the elastic fiber layer was observed in the mammary glands of the model system, whereas supplementation with 8% FBE containing water prevented this reduction. Moreover, we found that FBE supplementation prevented mammary epithelial cell invasion. Based on our findings, FBE might be a candidate material for a diet-based prevention of early breast cancer invasion.PMID:34012179 | PMC:PMC8116617 | DOI:10.1267/ahc.20-00029 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Junji Itou Akihiro Nakamura Hideki Hokazono Masakazu Toi Source Type: research

GLP-1 receptor - Do we really know what we're looking at?
Acta Histochem. 2021 May 18;123(5):151732. doi: 10.1016/j.acthis.2021.151732. Online ahead of print.NO ABSTRACTPMID:34015646 | DOI:10.1016/j.acthis.2021.151732 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Jan Homolak Ana Babic Perhoc Ana Knezovic Jelena Osmanovic Barilar Melita Salkovic-Petrisic Source Type: research

What Cyto- and Histochemistry Can Do to Crack the Sugar Code
Acta Histochem Cytochem. 2021 Apr 28;54(2):31-48. doi: 10.1267/ahc.21-00017. Epub 2021 Apr 17.ABSTRACTAs letters form the vocabulary of a language, biochemical 'symbols' (the building blocks of oligo- and polymers) make writing molecular messages possible. Compared to nucleotides and amino acids, sugars have chemical properties that facilitate to reach an unsurpassed level of oligomer diversity. These glycans are a part of the ubiquitous cellular glycoconjugates. Cyto- and histochemically, the glycans' structural complexity is mapped by glycophenotyping of cells and tissues using receptors ('readers', thus called lectins),...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Felix A Habermann Herbert Kaltner Alonso M Higuero Gabriel Garca Caballero Anna-Kristin Ludwig Joachim C Manning Jos Abad-Rodrguez Hans-Joachim Gabius Source Type: research

Development of Pathological Diagnosis Support System Using Micro-computed Tomography
In this study, we developed a system that supports the determination of the cutting position by visualizing and analyzing the internal structure of pathological material using micro-computed tomography (CT) before cutting. This system consists of a dedicated micro-CT and cutting support software. The micro-CT system has a fixture for fixing the target, enabling the scanning of easily deformable pathological materials. In the cutting support software, a function that interactively selects the extraction plane while displaying the volume rendering image and outputs a pseudo-histological image was implemented. Our results con...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Tomonari Hayakawa Atsushi Teramoto Yuka Kiriyama Tetsuya Tsukamoto Ayumi Yamada Kuniaki Saito Hiroshi Fujita Source Type: research

Altered Immunohistochemical Expression Patterns of HLA Class I during the Clinical Course of Cervical Intraepithelial Neoplasia
In conclusion, the immunohistochemical pattern of HLA class I expression is associated with the CIN grade, and it is alterable during the clinical course, especially in CIN2.PMID:34012177 | PMC:PMC8116618 | DOI:10.1267/ahc.21-00010 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Okuto Koguchi Haruna Nishimaki Yoko Nakanishi Hiroko Kobayashi Sumie Ohni Xiaoyan Tang Yoshiaki Kusumi Shinobu Masuda Source Type: research

Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts
Acta Histochem Cytochem. 2021 Apr 28;54(2):65-72. doi: 10.1267/ahc.21-00016. Epub 2021 Apr 17.ABSTRACTSpontaneous Raman spectroscopy, which senses changes in cellular contents of reduced cytochrome c, could be a powerful tool for label-free evaluation of ischemic hearts. However, undetermined is whether it is applicable to evaluation of myocardial viability in ischemic hearts. To address this issue, we investigated sequential changes in Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart before and during ligation of the left coronary artery and its subsequent release and re-ligation. Under ...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Koki Ikemoto Kosuke Hashimoto Yoshinori Harada Yasuaki Kumamoto Michiyo Hayakawa Kentaro Mochizuki Kazuhiko Matsuo Kenta Yashiro Hitoshi Yaku Tetsuro Takamatsu Hideo Tanaka Source Type: research

Supplementation with Fermented Barley Extract Prevents Mammary Epithelial Cell Invasion in an Early Breast Cancer Model
This study utilized the model system and investigated the effect of fermented barley extract (FBE), a food material. The elastic fiber layer is the outermost layer of the mammary duct. A reduction in the elastic fiber layer was observed in the mammary glands of the model system, whereas supplementation with 8% FBE containing water prevented this reduction. Moreover, we found that FBE supplementation prevented mammary epithelial cell invasion. Based on our findings, FBE might be a candidate material for a diet-based prevention of early breast cancer invasion.PMID:34012179 | PMC:PMC8116617 | DOI:10.1267/ahc.20-00029 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Junji Itou Akihiro Nakamura Hideki Hokazono Masakazu Toi Source Type: research

GLP-1 receptor - Do we really know what we're looking at?
Acta Histochem. 2021 May 17;123(5):151732. doi: 10.1016/j.acthis.2021.151732. Online ahead of print.NO ABSTRACTPMID:34015646 | DOI:10.1016/j.acthis.2021.151732 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Jan Homolak Ana Babic Perhoc Ana Knezovic Jelena Osmanovic Barilar Melita Salkovic-Petrisic Source Type: research

What Cyto- and Histochemistry Can Do to Crack the Sugar Code
Acta Histochem Cytochem. 2021 Apr 28;54(2):31-48. doi: 10.1267/ahc.21-00017. Epub 2021 Apr 17.ABSTRACTAs letters form the vocabulary of a language, biochemical 'symbols' (the building blocks of oligo- and polymers) make writing molecular messages possible. Compared to nucleotides and amino acids, sugars have chemical properties that facilitate to reach an unsurpassed level of oligomer diversity. These glycans are a part of the ubiquitous cellular glycoconjugates. Cyto- and histochemically, the glycans' structural complexity is mapped by glycophenotyping of cells and tissues using receptors ('readers', thus called lectins),...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Felix A Habermann Herbert Kaltner Alonso M Higuero Gabriel Garca Caballero Anna-Kristin Ludwig Joachim C Manning Jos Abad-Rodrguez Hans-Joachim Gabius Source Type: research

Development of Pathological Diagnosis Support System Using Micro-computed Tomography
In this study, we developed a system that supports the determination of the cutting position by visualizing and analyzing the internal structure of pathological material using micro-computed tomography (CT) before cutting. This system consists of a dedicated micro-CT and cutting support software. The micro-CT system has a fixture for fixing the target, enabling the scanning of easily deformable pathological materials. In the cutting support software, a function that interactively selects the extraction plane while displaying the volume rendering image and outputs a pseudo-histological image was implemented. Our results con...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Tomonari Hayakawa Atsushi Teramoto Yuka Kiriyama Tetsuya Tsukamoto Ayumi Yamada Kuniaki Saito Hiroshi Fujita Source Type: research

Altered Immunohistochemical Expression Patterns of HLA Class I during the Clinical Course of Cervical Intraepithelial Neoplasia
In conclusion, the immunohistochemical pattern of HLA class I expression is associated with the CIN grade, and it is alterable during the clinical course, especially in CIN2.PMID:34012177 | PMC:PMC8116618 | DOI:10.1267/ahc.21-00010 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Okuto Koguchi Haruna Nishimaki Yoko Nakanishi Hiroko Kobayashi Sumie Ohni Xiaoyan Tang Yoshiaki Kusumi Shinobu Masuda Source Type: research

Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts
Acta Histochem Cytochem. 2021 Apr 28;54(2):65-72. doi: 10.1267/ahc.21-00016. Epub 2021 Apr 17.ABSTRACTSpontaneous Raman spectroscopy, which senses changes in cellular contents of reduced cytochrome c, could be a powerful tool for label-free evaluation of ischemic hearts. However, undetermined is whether it is applicable to evaluation of myocardial viability in ischemic hearts. To address this issue, we investigated sequential changes in Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart before and during ligation of the left coronary artery and its subsequent release and re-ligation. Under ...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Koki Ikemoto Kosuke Hashimoto Yoshinori Harada Yasuaki Kumamoto Michiyo Hayakawa Kentaro Mochizuki Kazuhiko Matsuo Kenta Yashiro Hitoshi Yaku Tetsuro Takamatsu Hideo Tanaka Source Type: research

Supplementation with Fermented Barley Extract Prevents Mammary Epithelial Cell Invasion in an Early Breast Cancer Model
This study utilized the model system and investigated the effect of fermented barley extract (FBE), a food material. The elastic fiber layer is the outermost layer of the mammary duct. A reduction in the elastic fiber layer was observed in the mammary glands of the model system, whereas supplementation with 8% FBE containing water prevented this reduction. Moreover, we found that FBE supplementation prevented mammary epithelial cell invasion. Based on our findings, FBE might be a candidate material for a diet-based prevention of early breast cancer invasion.PMID:34012179 | PMC:PMC8116617 | DOI:10.1267/ahc.20-00029 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Junji Itou Akihiro Nakamura Hideki Hokazono Masakazu Toi Source Type: research

GLP-1 receptor - Do we really know what we're looking at?
Acta Histochem. 2021 May 17;123(5):151732. doi: 10.1016/j.acthis.2021.151732. Online ahead of print.NO ABSTRACTPMID:34015646 | DOI:10.1016/j.acthis.2021.151732 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Jan Homolak Ana Babic Perhoc Ana Knezovic Jelena Osmanovic Barilar Melita Salkovic-Petrisic Source Type: research

What Cyto- and Histochemistry Can Do to Crack the Sugar Code
Acta Histochem Cytochem. 2021 Apr 28;54(2):31-48. doi: 10.1267/ahc.21-00017. Epub 2021 Apr 17.ABSTRACTAs letters form the vocabulary of a language, biochemical 'symbols' (the building blocks of oligo- and polymers) make writing molecular messages possible. Compared to nucleotides and amino acids, sugars have chemical properties that facilitate to reach an unsurpassed level of oligomer diversity. These glycans are a part of the ubiquitous cellular glycoconjugates. Cyto- and histochemically, the glycans' structural complexity is mapped by glycophenotyping of cells and tissues using receptors ('readers', thus called lectins),...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Felix A Habermann Herbert Kaltner Alonso M Higuero Gabriel Garca Caballero Anna-Kristin Ludwig Joachim C Manning Jos Abad-Rodrguez Hans-Joachim Gabius Source Type: research

Development of Pathological Diagnosis Support System Using Micro-computed Tomography
In this study, we developed a system that supports the determination of the cutting position by visualizing and analyzing the internal structure of pathological material using micro-computed tomography (CT) before cutting. This system consists of a dedicated micro-CT and cutting support software. The micro-CT system has a fixture for fixing the target, enabling the scanning of easily deformable pathological materials. In the cutting support software, a function that interactively selects the extraction plane while displaying the volume rendering image and outputs a pseudo-histological image was implemented. Our results con...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Tomonari Hayakawa Atsushi Teramoto Yuka Kiriyama Tetsuya Tsukamoto Ayumi Yamada Kuniaki Saito Hiroshi Fujita Source Type: research

Altered Immunohistochemical Expression Patterns of HLA Class I during the Clinical Course of Cervical Intraepithelial Neoplasia
In conclusion, the immunohistochemical pattern of HLA class I expression is associated with the CIN grade, and it is alterable during the clinical course, especially in CIN2.PMID:34012177 | PMC:PMC8116618 | DOI:10.1267/ahc.21-00010 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Okuto Koguchi Haruna Nishimaki Yoko Nakanishi Hiroko Kobayashi Sumie Ohni Xiaoyan Tang Yoshiaki Kusumi Shinobu Masuda Source Type: research

Raman Spectroscopic Assessment of Myocardial Viability in Langendorff-Perfused Ischemic Rat Hearts
Acta Histochem Cytochem. 2021 Apr 28;54(2):65-72. doi: 10.1267/ahc.21-00016. Epub 2021 Apr 17.ABSTRACTSpontaneous Raman spectroscopy, which senses changes in cellular contents of reduced cytochrome c, could be a powerful tool for label-free evaluation of ischemic hearts. However, undetermined is whether it is applicable to evaluation of myocardial viability in ischemic hearts. To address this issue, we investigated sequential changes in Raman spectra of the subepicardial myocardium in the Langendorff-perfused rat heart before and during ligation of the left coronary artery and its subsequent release and re-ligation. Under ...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Koki Ikemoto Kosuke Hashimoto Yoshinori Harada Yasuaki Kumamoto Michiyo Hayakawa Kentaro Mochizuki Kazuhiko Matsuo Kenta Yashiro Hitoshi Yaku Tetsuro Takamatsu Hideo Tanaka Source Type: research

Supplementation with Fermented Barley Extract Prevents Mammary Epithelial Cell Invasion in an Early Breast Cancer Model
This study utilized the model system and investigated the effect of fermented barley extract (FBE), a food material. The elastic fiber layer is the outermost layer of the mammary duct. A reduction in the elastic fiber layer was observed in the mammary glands of the model system, whereas supplementation with 8% FBE containing water prevented this reduction. Moreover, we found that FBE supplementation prevented mammary epithelial cell invasion. Based on our findings, FBE might be a candidate material for a diet-based prevention of early breast cancer invasion.PMID:34012179 | PMC:PMC8116617 | DOI:10.1267/ahc.20-00029 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Junji Itou Akihiro Nakamura Hideki Hokazono Masakazu Toi Source Type: research

GLP-1 receptor - Do we really know what we're looking at?
Acta Histochem. 2021 May 17;123(5):151732. doi: 10.1016/j.acthis.2021.151732. Online ahead of print.NO ABSTRACTPMID:34015646 | DOI:10.1016/j.acthis.2021.151732 (Source: Acta Histochemica)
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Jan Homolak Ana Babic Perhoc Ana Knezovic Jelena Osmanovic Barilar Melita Salkovic-Petrisic Source Type: research

What Cyto- and Histochemistry Can Do to Crack the Sugar Code
Acta Histochem Cytochem. 2021 Apr 28;54(2):31-48. doi: 10.1267/ahc.21-00017. Epub 2021 Apr 17.ABSTRACTAs letters form the vocabulary of a language, biochemical 'symbols' (the building blocks of oligo- and polymers) make writing molecular messages possible. Compared to nucleotides and amino acids, sugars have chemical properties that facilitate to reach an unsurpassed level of oligomer diversity. These glycans are a part of the ubiquitous cellular glycoconjugates. Cyto- and histochemically, the glycans' structural complexity is mapped by glycophenotyping of cells and tissues using receptors ('readers', thus called lectins),...
Source: Acta Histochemica - May 20, 2021 Category: Biochemistry Authors: Felix A Habermann Herbert Kaltner Alonso M Higuero Gabriel Garca Caballero Anna-Kristin Ludwig Joachim C Manning Jos Abad-Rodrguez Hans-Joachim Gabius Source Type: research