Beneficial Effect of Erythropoietin on Ameliorating Propionic Acid-Induced Autistic-Like Features in Young Rats
This study investigated the possible beneficial effects of erythropoietin (EPO) on experimental autistic-like behaviors induced by propionic acid (PPA). Twenty-four rats were distributed into three groups: (i) control; (ii) PPA_Gp: daily injected subcutaneously with PPA for five consecutive days; PPA+EPO-Gp: injected with PPA, then received intraperitoneal injection of EPO once daily for two weeks. Behavioral changes in the rats were assessed. Specimens from the cerebellar hemispheres were subjected to histological and ultrastructure examination, immunohistochemistry for glial fibrillary acidic protein (GFAP) and calbindin...
Source: Acta Histochemica - November 16, 2023 Category: Biochemistry Authors: Sara A Hosny Alshaymaa M Abdelmenem Taha Azouz Samaa S Kamar Asmaa M ShamsEldeen Asmaa A El-Shafei Source Type: research
Immunolocalization of lix1 in the regenerating tail of lizard indicates that the protein is mainly present in the nervous tissue
CONCLUSIONS: Since the spinal cord is essential to stimulate tail regeneration it is hypothesized that the lix1 protein is part of the signaling or growing factors produced from the regenerating spinal cord that are needed for tail regeneration of the lizard tail.PMID:37948784 | DOI:10.1016/j.acthis.2023.152113 (Source: Acta Histochemica)
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: Lorenzo Alibardi Source Type: research
Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats
This study evaluated the effects of different concentrations of Kp-10 on in vitro osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs) extracted from the bone marrow (BM) of adult Wistar rats. Two-month-old female rats were euthanized to extract BM from long bones to obtain MSCs. Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL. After induction of osteogenic differentiation, cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase activity, collagen synt...
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: La ís Bitencourt Guimarães Daniel Portela Dias Machado Beatriz Ferreira Carvalho Versiani Caldeira Larissa Tiemi Matuzake Vieira Gabriela Alves Santos Fabiana Rocha Ara újo Leonardo Teot ônio Machado Dawidson Assis Gomes Nat ália de Melo Ocarino Rog Source Type: research
Immunolocalization of lix1 in the regenerating tail of lizard indicates that the protein is mainly present in the nervous tissue
CONCLUSIONS: Since the spinal cord is essential to stimulate tail regeneration it is hypothesized that the lix1 protein is part of the signaling or growing factors produced from the regenerating spinal cord that are needed for tail regeneration of the lizard tail.PMID:37948784 | DOI:10.1016/j.acthis.2023.152113 (Source: Acta Histochemica)
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: Lorenzo Alibardi Source Type: research
Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats
This study evaluated the effects of different concentrations of Kp-10 on in vitro osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs) extracted from the bone marrow (BM) of adult Wistar rats. Two-month-old female rats were euthanized to extract BM from long bones to obtain MSCs. Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL. After induction of osteogenic differentiation, cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase activity, collagen synt...
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: La ís Bitencourt Guimarães Daniel Portela Dias Machado Beatriz Ferreira Carvalho Versiani Caldeira Larissa Tiemi Matuzake Vieira Gabriela Alves Santos Fabiana Rocha Ara újo Leonardo Teot ônio Machado Dawidson Assis Gomes Nat ália de Melo Ocarino Rog Source Type: research
Immunolocalization of lix1 in the regenerating tail of lizard indicates that the protein is mainly present in the nervous tissue
CONCLUSIONS: Since the spinal cord is essential to stimulate tail regeneration it is hypothesized that the lix1 protein is part of the signaling or growing factors produced from the regenerating spinal cord that are needed for tail regeneration of the lizard tail.PMID:37948784 | DOI:10.1016/j.acthis.2023.152113 (Source: Acta Histochemica)
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: Lorenzo Alibardi Source Type: research
Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats
This study evaluated the effects of different concentrations of Kp-10 on in vitro osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs) extracted from the bone marrow (BM) of adult Wistar rats. Two-month-old female rats were euthanized to extract BM from long bones to obtain MSCs. Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL. After induction of osteogenic differentiation, cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase activity, collagen synt...
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: La ís Bitencourt Guimarães Daniel Portela Dias Machado Beatriz Ferreira Carvalho Versiani Caldeira Larissa Tiemi Matuzake Vieira Gabriela Alves Santos Fabiana Rocha Ara újo Leonardo Teot ônio Machado Dawidson Assis Gomes Nat ália de Melo Ocarino Rog Source Type: research
Immunolocalization of lix1 in the regenerating tail of lizard indicates that the protein is mainly present in the nervous tissue
CONCLUSIONS: Since the spinal cord is essential to stimulate tail regeneration it is hypothesized that the lix1 protein is part of the signaling or growing factors produced from the regenerating spinal cord that are needed for tail regeneration of the lizard tail.PMID:37948784 | DOI:10.1016/j.acthis.2023.152113 (Source: Acta Histochemica)
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: Lorenzo Alibardi Source Type: research
Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats
This study evaluated the effects of different concentrations of Kp-10 on in vitro osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs) extracted from the bone marrow (BM) of adult Wistar rats. Two-month-old female rats were euthanized to extract BM from long bones to obtain MSCs. Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL. After induction of osteogenic differentiation, cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase activity, collagen synt...
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: La ís Bitencourt Guimarães Daniel Portela Dias Machado Beatriz Ferreira Carvalho Versiani Caldeira Larissa Tiemi Matuzake Vieira Gabriela Alves Santos Fabiana Rocha Ara újo Leonardo Teot ônio Machado Dawidson Assis Gomes Nat ália de Melo Ocarino Rog Source Type: research
Immunolocalization of lix1 in the regenerating tail of lizard indicates that the protein is mainly present in the nervous tissue
CONCLUSIONS: Since the spinal cord is essential to stimulate tail regeneration it is hypothesized that the lix1 protein is part of the signaling or growing factors produced from the regenerating spinal cord that are needed for tail regeneration of the lizard tail.PMID:37948784 | DOI:10.1016/j.acthis.2023.152113 (Source: Acta Histochemica)
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: Lorenzo Alibardi Source Type: research
Kisspeptin (Kp-10) inhibits in vitro osteogenic differentiation of multipotent mesenchymal stromal cells extracted from the bone marrow of adult rats
This study evaluated the effects of different concentrations of Kp-10 on in vitro osteogenic differentiation of multipotent mesenchymal stromal cells (MSCs) extracted from the bone marrow (BM) of adult Wistar rats. Two-month-old female rats were euthanized to extract BM from long bones to obtain MSCs. Four experimental groups were established in vitro: a control and Kp-10 at concentrations of 0.01, 0.05 and, 0.1 µg/mL. After induction of osteogenic differentiation, cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase activity, collagen synt...
Source: Acta Histochemica - November 10, 2023 Category: Biochemistry Authors: La ís Bitencourt Guimarães Daniel Portela Dias Machado Beatriz Ferreira Carvalho Versiani Caldeira Larissa Tiemi Matuzake Vieira Gabriela Alves Santos Fabiana Rocha Ara újo Leonardo Teot ônio Machado Dawidson Assis Gomes Nat ália de Melo Ocarino Rog Source Type: research
In vitro validation: GLY alleviates UV-induced corneal epithelial damage through the HMGB1-TLR/MyD88-NF- κB signaling pathway
Acta Histochem. 2023 Nov 6;125(8):152111. doi: 10.1016/j.acthis.2023.152111. Online ahead of print.ABSTRACTUV-induced corneal damage is a common ocular surface injury that usually leads to corneal lesions causing persistent inflammation. High mobility group box 1 (HMGB1) is identified as an inflammatory alarm in various tissue injuries. Here, this study first evaluates the repair effect of the HMGB1-selective inhibitor GLY in UV-induced corneal damage; Secondly, the inhibitory effect of GLY on UV-induced corneal damage induced inflammation and the potential therapeutic mechanism of GLY were studied. GLY effectively attenua...
Source: Acta Histochemica - November 8, 2023 Category: Biochemistry Authors: XinYi Chen XiaoXiao Zheng Ting Shen Ting He YangQi Zhao Yi Dong Source Type: research
In vitro validation: GLY alleviates UV-induced corneal epithelial damage through the HMGB1-TLR/MyD88-NF- κB signaling pathway
Acta Histochem. 2023 Nov 6;125(8):152111. doi: 10.1016/j.acthis.2023.152111. Online ahead of print.ABSTRACTUV-induced corneal damage is a common ocular surface injury that usually leads to corneal lesions causing persistent inflammation. High mobility group box 1 (HMGB1) is identified as an inflammatory alarm in various tissue injuries. Here, this study first evaluates the repair effect of the HMGB1-selective inhibitor GLY in UV-induced corneal damage; Secondly, the inhibitory effect of GLY on UV-induced corneal damage induced inflammation and the potential therapeutic mechanism of GLY were studied. GLY effectively attenua...
Source: Acta Histochemica - November 8, 2023 Category: Biochemistry Authors: XinYi Chen XiaoXiao Zheng Ting Shen Ting He YangQi Zhao Yi Dong Source Type: research
The effect on m < sup > 6 < /sup > A methylation writer complex by the reduced MATR3 in pterygium
Acta Histochem. 2023 Oct 30;125(8):152101. doi: 10.1016/j.acthis.2023.152101. Online ahead of print.ABSTRACTPterygium is a common eye surface disease with high recurrence and unclear pathogenesis. In current study, RNA sequencing was conducted in 6 pairs of human pterygium and conjunctival tissues, and Matr3 as a novel candidate gene was significantly reduced in pterygium compared to control tissues. Moreover, immunoprecipitation was performed to pull down MATR3, and WTAP specially interacting with MATR3 in control but not pterygium was identified by mass spectrum. Immunoprecipitation was performed to validate the interact...
Source: Acta Histochemica - November 1, 2023 Category: Biochemistry Authors: Qianqian Guo Shichao Han Source Type: research
The effect on m < sup > 6 < /sup > A methylation writer complex by the reduced MATR3 in pterygium
Acta Histochem. 2023 Oct 30;125(8):152101. doi: 10.1016/j.acthis.2023.152101. Online ahead of print.ABSTRACTPterygium is a common eye surface disease with high recurrence and unclear pathogenesis. In current study, RNA sequencing was conducted in 6 pairs of human pterygium and conjunctival tissues, and Matr3 as a novel candidate gene was significantly reduced in pterygium compared to control tissues. Moreover, immunoprecipitation was performed to pull down MATR3, and WTAP specially interacting with MATR3 in control but not pterygium was identified by mass spectrum. Immunoprecipitation was performed to validate the interact...
Source: Acta Histochemica - November 1, 2023 Category: Biochemistry Authors: Qianqian Guo Shichao Han Source Type: research
