Tissue-Targeted Transcriptomics Reveals SEMA3D Control of Hypoglossal Nerve Projection to Mouse Tongue Primordia
Acta Histochem Cytochem. 2024 Feb 29;57(1):35-46. doi: 10.1267/ahc.23-00073. Epub 2024 Feb 23.ABSTRACTThe mouse hypoglossal nerve originates in the occipital motor nuclei at embryonic day (E)10.5 and projects a long distance, reaching the vicinity of the tongue primordia, the lateral lingual swellings, at E11.5. However, the details of how the hypoglossal nerve correctly projects to the primordia are poorly understood. To investigate the molecular basis of hypoglossal nerve elongation, we used a novel transcriptomic approach using the ROKU method. The ROKU algorithm identified 3825 genes specific for lateral lingual swelli...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Taisuke Hani Kazuya Fujita Tomoo Kudo Yuji Taya Kaori Sato Yuuichi Soeno Source Type: research
Aquaporin-5 Protein Is Selectively Reduced in Rat Parotid Glands under Conditions of Fasting or a Liquid Diet
We examined their expression changes in rat parotid glands under reduced mastication. Rats were either fed regular chow as a control group, fasted for 48 hr or fed a liquid diet for 48 hr or 1 week to reduce mastication. The parotid glands were then resected to analyze the protein and mRNA levels by immunofluorescence, immunoblotting, and reverse-transcription quantitative PCR (RT-qPCR). AQP5 protein was significantly decreased in both liquid diet groups and the fasting group but its mRNA levels showed no apparent changes compared with the control group. The protein and mRNA levels of TMEM16A and NKCC1 showed no significan...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Yoshie Fujita Akie Taniguchi Hanako Yamamoto Hideru Obinata Hiroshi Kogo Akiko Iizuka-Kogo Maiko Ikezawa Yukiko Tajika Satoshi Yokoo Toshiyuki Matsuzaki Source Type: research
Glucose Induces ER Stress Response-Mediated Peritoneal Mesothelial Cell Death
Acta Histochem Cytochem. 2024 Feb 29;57(1):7-14. doi: 10.1267/ahc.23-00050. Epub 2024 Feb 23.ABSTRACTPeritoneal dialysis (PD) fluid, which contains a high concentration of glucose, is involved in peritoneal damage after long-term use. The mechanisms through which glucose induces damage to the mesothelium have not been clearly elucidated. Although, endoplasmic reticulum (ER) stress response is associated with several diseases, the involvement of ER stress in peritoneal damage has not yet been demonstrated. Primary-cultured rat peritoneal mesothelial cells (RPMCs) and rat PD model were used to investigate the influence of gl...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Junichi Nakamata Hiroyuki Morimoto Ryoko Baba Keiji Kokubu Tetsu Miyamoto Source Type: research
The Effects of Corticosteroid Administration and Treadmill Exercise on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in Rats
Acta Histochem Cytochem. 2024 Feb 29;57(1):47-55. doi: 10.1267/ahc.23-00068. Epub 2024 Feb 23.ABSTRACTWe aimed to investigate the effects of short-term corticosteroid administration after anterior cruciate ligament (ACL) reconstruction on marrow adipose tissue (MAT) and trabecular bone mass, as well as to examine whether treadmill exercise can mitigate MAT increase and trabecular bone deterioration caused by corticosteroid. ACL-reconstructed rats were divided into groups: no intervention, daily treadmill exercise (60 min/day), administration of the steroidal drug dexamethasone (250 μg/kg on days 0-5, 7, and 9 post-operati...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Akinori Kaneguchi Kaoru Yamaoka Junya Ozawa Source Type: research
The Mouse Model of Internal Capsule Demyelination: A Novel Tool for Investigating Motor Functional Changes Caused by Demyelination and for Evaluating Drugs That Promote Remyelination
Acta Histochem Cytochem. 2024 Feb 29;57(1):1-5. doi: 10.1267/ahc.24-00005. Epub 2024 Feb 23.ABSTRACTMultiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system, characterized by remyelination failure and axonal dysfunction. Remyelination by oligodendrocytes is critical for improvement of neurological deficits associated with demyelination. Rodent models of demyelination are frequently used to develop and evaluate therapies for MS. However, a suitable mouse model for assessing remyelination-associated recovery of motor functions is currently unavailable. In this review, we describe the dev...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Reiji Yamazaki Nobuhiko Ohno Source Type: research
Effects of Weight Bearing on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in the Rat Proximal Tibial Epiphysis
Acta Histochem Cytochem. 2024 Feb 29;57(1):15-24. doi: 10.1267/ahc.23-00060. Epub 2024 Feb 23.ABSTRACTThe effects of mechanical unloading after anterior cruciate ligament (ACL) reconstruction on bone and marrow adipose tissue (MAT) are unclear. We investigated weight bearing effects on bone and MAT after ACL reconstruction. Rats underwent unilateral knee ACL transection and reconstruction, followed by hindlimb unloading (non-weight bearing), no intervention (low-weight bearing, the hindlimb standing time ratio (STR; operated/contralateral) during treadmill locomotion ranging from 0.55 to 0.91), or sustained morphine admini...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Akinori Kaneguchi Kaoru Yamaoka Junya Ozawa Source Type: research
Tissue-Targeted Transcriptomics Reveals SEMA3D Control of Hypoglossal Nerve Projection to Mouse Tongue Primordia
Acta Histochem Cytochem. 2024 Feb 29;57(1):35-46. doi: 10.1267/ahc.23-00073. Epub 2024 Feb 23.ABSTRACTThe mouse hypoglossal nerve originates in the occipital motor nuclei at embryonic day (E)10.5 and projects a long distance, reaching the vicinity of the tongue primordia, the lateral lingual swellings, at E11.5. However, the details of how the hypoglossal nerve correctly projects to the primordia are poorly understood. To investigate the molecular basis of hypoglossal nerve elongation, we used a novel transcriptomic approach using the ROKU method. The ROKU algorithm identified 3825 genes specific for lateral lingual swelli...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Taisuke Hani Kazuya Fujita Tomoo Kudo Yuji Taya Kaori Sato Yuuichi Soeno Source Type: research
Aquaporin-5 Protein Is Selectively Reduced in Rat Parotid Glands under Conditions of Fasting or a Liquid Diet
We examined their expression changes in rat parotid glands under reduced mastication. Rats were either fed regular chow as a control group, fasted for 48 hr or fed a liquid diet for 48 hr or 1 week to reduce mastication. The parotid glands were then resected to analyze the protein and mRNA levels by immunofluorescence, immunoblotting, and reverse-transcription quantitative PCR (RT-qPCR). AQP5 protein was significantly decreased in both liquid diet groups and the fasting group but its mRNA levels showed no apparent changes compared with the control group. The protein and mRNA levels of TMEM16A and NKCC1 showed no significan...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Yoshie Fujita Akie Taniguchi Hanako Yamamoto Hideru Obinata Hiroshi Kogo Akiko Iizuka-Kogo Maiko Ikezawa Yukiko Tajika Satoshi Yokoo Toshiyuki Matsuzaki Source Type: research
Glucose Induces ER Stress Response-Mediated Peritoneal Mesothelial Cell Death
Acta Histochem Cytochem. 2024 Feb 29;57(1):7-14. doi: 10.1267/ahc.23-00050. Epub 2024 Feb 23.ABSTRACTPeritoneal dialysis (PD) fluid, which contains a high concentration of glucose, is involved in peritoneal damage after long-term use. The mechanisms through which glucose induces damage to the mesothelium have not been clearly elucidated. Although, endoplasmic reticulum (ER) stress response is associated with several diseases, the involvement of ER stress in peritoneal damage has not yet been demonstrated. Primary-cultured rat peritoneal mesothelial cells (RPMCs) and rat PD model were used to investigate the influence of gl...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Junichi Nakamata Hiroyuki Morimoto Ryoko Baba Keiji Kokubu Tetsu Miyamoto Source Type: research
The Effects of Corticosteroid Administration and Treadmill Exercise on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in Rats
Acta Histochem Cytochem. 2024 Feb 29;57(1):47-55. doi: 10.1267/ahc.23-00068. Epub 2024 Feb 23.ABSTRACTWe aimed to investigate the effects of short-term corticosteroid administration after anterior cruciate ligament (ACL) reconstruction on marrow adipose tissue (MAT) and trabecular bone mass, as well as to examine whether treadmill exercise can mitigate MAT increase and trabecular bone deterioration caused by corticosteroid. ACL-reconstructed rats were divided into groups: no intervention, daily treadmill exercise (60 min/day), administration of the steroidal drug dexamethasone (250 μg/kg on days 0-5, 7, and 9 post-operati...
Source: Acta Histochemica - March 11, 2024 Category: Biochemistry Authors: Akinori Kaneguchi Kaoru Yamaoka Junya Ozawa Source Type: research
Investigation of the differentiation potential of pericyte cells as an alternative source of mesenchymal stem cells
In this study, therefore, we conducted molecular level examinations to determine whether pericytes, specialized cell communities situated near blood vessels, could serve as a substitute for human bone marrow-derived mesenchymal stem cells (hBM-MSCs). In this context, the potential application of pericytes surrounds the vessels when MSCs are insufficient for functional purposes.METHODS: The pericytes utilized in this investigation were derived from the placenta and characterized at the third passage. Similarly, the hBM-MSCs were also characterized at the third passage. The pluripotent properties of the two cell types were a...
Source: Acta Histochemica - March 3, 2024 Category: Biochemistry Authors: Selen Polat Yusufhan Yazir G ökhan Duruksu Kamil Can Kili ç Serap Mert G ülçin Gacar B üşra Öncel Duman Zehra Seda Halbuto ğullari Source Type: research
Investigation of the differentiation potential of pericyte cells as an alternative source of mesenchymal stem cells
In this study, therefore, we conducted molecular level examinations to determine whether pericytes, specialized cell communities situated near blood vessels, could serve as a substitute for human bone marrow-derived mesenchymal stem cells (hBM-MSCs). In this context, the potential application of pericytes surrounds the vessels when MSCs are insufficient for functional purposes.METHODS: The pericytes utilized in this investigation were derived from the placenta and characterized at the third passage. Similarly, the hBM-MSCs were also characterized at the third passage. The pluripotent properties of the two cell types were a...
Source: Acta Histochemica - March 3, 2024 Category: Biochemistry Authors: Selen Polat Yusufhan Yazir G ökhan Duruksu Kamil Can Kili ç Serap Mert G ülçin Gacar B üşra Öncel Duman Zehra Seda Halbuto ğullari Source Type: research
Investigation of the differentiation potential of pericyte cells as an alternative source of mesenchymal stem cells
In this study, therefore, we conducted molecular level examinations to determine whether pericytes, specialized cell communities situated near blood vessels, could serve as a substitute for human bone marrow-derived mesenchymal stem cells (hBM-MSCs). In this context, the potential application of pericytes surrounds the vessels when MSCs are insufficient for functional purposes.METHODS: The pericytes utilized in this investigation were derived from the placenta and characterized at the third passage. Similarly, the hBM-MSCs were also characterized at the third passage. The pluripotent properties of the two cell types were a...
Source: Acta Histochemica - March 3, 2024 Category: Biochemistry Authors: Selen Polat Yusufhan Yazir G ökhan Duruksu Kamil Can Kili ç Serap Mert G ülçin Gacar B üşra Öncel Duman Zehra Seda Halbuto ğullari Source Type: research
Investigation of the differentiation potential of pericyte cells as an alternative source of mesenchymal stem cells
In this study, therefore, we conducted molecular level examinations to determine whether pericytes, specialized cell communities situated near blood vessels, could serve as a substitute for human bone marrow-derived mesenchymal stem cells (hBM-MSCs). In this context, the potential application of pericytes surrounds the vessels when MSCs are insufficient for functional purposes.METHODS: The pericytes utilized in this investigation were derived from the placenta and characterized at the third passage. Similarly, the hBM-MSCs were also characterized at the third passage. The pluripotent properties of the two cell types were a...
Source: Acta Histochemica - March 3, 2024 Category: Biochemistry Authors: Selen Polat Yusufhan Yazir G ökhan Duruksu Kamil Can Kili ç Serap Mert G ülçin Gacar B üşra Öncel Duman Zehra Seda Halbuto ğullari Source Type: research
Investigation of the differentiation potential of pericyte cells as an alternative source of mesenchymal stem cells
In this study, therefore, we conducted molecular level examinations to determine whether pericytes, specialized cell communities situated near blood vessels, could serve as a substitute for human bone marrow-derived mesenchymal stem cells (hBM-MSCs). In this context, the potential application of pericytes surrounds the vessels when MSCs are insufficient for functional purposes.METHODS: The pericytes utilized in this investigation were derived from the placenta and characterized at the third passage. Similarly, the hBM-MSCs were also characterized at the third passage. The pluripotent properties of the two cell types were a...
Source: Acta Histochemica - March 3, 2024 Category: Biochemistry Authors: Selen Polat Yusufhan Yazir G ökhan Duruksu Kamil Can Kili ç Serap Mert G ülçin Gacar B üşra Öncel Duman Zehra Seda Halbuto ğullari Source Type: research
