Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection
Appl Microbiol Biotechnol. 2024 Mar 13;108(1):261. doi: 10.1007/s00253-024-13102-9.ABSTRACTNon-coding RNA (ncRNA) plays a vital part in the regulation of immune responses, growth, and development in plants and animals. Here, the identification, characteristic analysis, and molecular verification of circRNAs in Apis cerana cerana worker larval guts were conducted, followed by in-depth investigation of the expression pattern of larval circRNAs during Ascosphaera apis infection and exploration of the potential regulatory part of differentially expressed circRNAs (DEcircRNAs) in host immune responses. A total of 3178 circRNAs ...
Source: Applied Microbiology and Biotechnology - March 13, 2024 Category: Microbiology Authors: Rui Guo Kaiyao Zhang He Zang Sijia Guo Xiaoyu Liu Xin Jing Yuxuan Song Kunze Li Ying Wu Haibing Jiang Zhongmin Fu Dafu Chen Source Type: research
Social life results in social stress protection: a novel concept to explain individual life-history patterns in social insects
We describe major categories of stress and how a colonial lifestyle may protect social insects, particularly against temporary peaks of extreme stress. We use the honey bee (Apis mellifera L.) to illustrate how patterns of life expectancy may be explained by social stress protection and how modern beekeeping practices can disrupt social stress protection. We conclude that the broad concept of social stress protection requires rigorous empirical testing because it may have implications for our general understanding of social evolution and specifically for improving honey bee health.PMID:38468146 | DOI:10.1111/brv.13074 (Sou...
Source: Biological Reviews of the Cambridge Philosophical Society - March 12, 2024 Category: Biology Authors: Alexander Walton Jacob J Herman Olav Rueppell Source Type: research
Social life results in social stress protection: a novel concept to explain individual life-history patterns in social insects
We describe major categories of stress and how a colonial lifestyle may protect social insects, particularly against temporary peaks of extreme stress. We use the honey bee (Apis mellifera L.) to illustrate how patterns of life expectancy may be explained by social stress protection and how modern beekeeping practices can disrupt social stress protection. We conclude that the broad concept of social stress protection requires rigorous empirical testing because it may have implications for our general understanding of social evolution and specifically for improving honey bee health.PMID:38468146 | DOI:10.1111/brv.13074 (Sou...
Source: Biological Reviews of the Cambridge Philosophical Society - March 12, 2024 Category: Biology Authors: Alexander Walton Jacob J Herman Olav Rueppell Source Type: research
Exploring honey bee toxicological data as a proxy for assessing dimethoate sensitivity in stingless bees
Chemosphere. 2024 Mar 8:141652. doi: 10.1016/j.chemosphere.2024.141652. Online ahead of print.ABSTRACTThe high diversity and distinctive characteristics of stingless bees pose challenges in utilizing toxicity test results for agrochemical registrations. Toxicity assessments were performed on 15 stingless bee species, along with the honey bee, using the insecticide dimethoate, following adapted OECD protocols. Median lethal doses over 24 h (24 h-LD50) were determined for exposure routes (acute oral or contact) and species. Species sensitivity distribution (SSD) curves were constructed and the 5% hazard doses (HD5) were esti...
Source: Chemosphere - March 10, 2024 Category: Chemistry Authors: Andreza Ribas Lorena Lisbetd Botina Renan Dos Santos Ara újo Mateus Lordelo Vidigal Beatriz Cristina da Silva Alves Gustavo Ferreira Martins Source Type: research
Exploring honey bee toxicological data as a proxy for assessing dimethoate sensitivity in stingless bees
Chemosphere. 2024 Mar 8:141652. doi: 10.1016/j.chemosphere.2024.141652. Online ahead of print.ABSTRACTThe high diversity and distinctive characteristics of stingless bees pose challenges in utilizing toxicity test results for agrochemical registrations. Toxicity assessments were performed on 15 stingless bee species, along with the honey bee, using the insecticide dimethoate, following adapted OECD protocols. Median lethal doses over 24 h (24 h-LD50) were determined for exposure routes (acute oral or contact) and species. Species sensitivity distribution (SSD) curves were constructed and the 5% hazard doses (HD5) were esti...
Source: Chemosphere - March 10, 2024 Category: Chemistry Authors: Andreza Ribas Lorena Lisbetd Botina Renan Dos Santos Ara újo Mateus Lordelo Vidigal Beatriz Cristina da Silva Alves Gustavo Ferreira Martins Source Type: research
Exploring honey bee toxicological data as a proxy for assessing dimethoate sensitivity in stingless bees
Chemosphere. 2024 Mar 8:141652. doi: 10.1016/j.chemosphere.2024.141652. Online ahead of print.ABSTRACTThe high diversity and distinctive characteristics of stingless bees pose challenges in utilizing toxicity test results for agrochemical registrations. Toxicity assessments were performed on 15 stingless bee species, along with the honey bee, using the insecticide dimethoate, following adapted OECD protocols. Median lethal doses over 24 h (24 h-LD50) were determined for exposure routes (acute oral or contact) and species. Species sensitivity distribution (SSD) curves were constructed and the 5% hazard doses (HD5) were esti...
Source: Chemosphere - March 10, 2024 Category: Chemistry Authors: Andreza Ribas Lorena Lisbetd Botina Renan Dos Santos Ara újo Mateus Lordelo Vidigal Beatriz Cristina da Silva Alves Gustavo Ferreira Martins Source Type: research
Microwave-induced combustion for further determination of potentially toxic elements in honey by ICP-MS
Anal Bioanal Chem. 2024 Mar 9. doi: 10.1007/s00216-024-05235-7. Online ahead of print.ABSTRACTMicrowave-induced combustion (MIC) was proposed in this study for honey decomposition aiming for As, Cd, Hg, and Pb determination by inductively coupled plasma mass spectrometry (ICP-MS). Sample mass (up to 1.0 g), absorbing solution (0.5 to 14.4 mol L-1 HNO3, and H2O), heating program, and combustion aids were evaluated. The Eurachem guidelines were used for method validation. The proposed method enabled combustion of a high sample mass (0.8 g of honey, with 0.4 g of microcrystalline cellulose and 100 µL of 6 mol L-1 NH4NO3) usi...
Source: Analytical and Bioanalytical Chemistry - March 9, 2024 Category: Chemistry Authors: Vitoria H Cauduro Camila M A C Alves Mariele S Nascimento Gabriel T Druzian Fernanda P Balbinot Marcia F Mesko Erico M M Flores Source Type: research
Determining pyroxasulfone herbicide in honey samples using liquid-liquid extraction with low temperature purification (LLE-LTP)
Talanta. 2024 Mar 2;273:125870. doi: 10.1016/j.talanta.2024.125870. Online ahead of print.ABSTRACTPyroxasulfone is a selective, systemic, pre-emergence herbicide which acts to inhibit weeds in potato, coffee, sugar cane, eucalyptus, and soybean plantations, among others. This active ingredient was classified by Brazilian legislation as a very dangerous product for the environment, and to date there are no studies involving the development of extraction methods for monitoring this compound in environmental matrices. Therefore, the objective of this study was to optimize and validate liquid-liquid extraction with low tempera...
Source: Talanta - March 9, 2024 Category: Chemistry Authors: Luma Soares Costa Carolina Freitas Schettino L ázaro Chaves Sicupira Gevany Paulino de Pinho Flaviano Oliveira Silv ério Source Type: research
Microwave-induced combustion for further determination of potentially toxic elements in honey by ICP-MS
Anal Bioanal Chem. 2024 Mar 9. doi: 10.1007/s00216-024-05235-7. Online ahead of print.ABSTRACTMicrowave-induced combustion (MIC) was proposed in this study for honey decomposition aiming for As, Cd, Hg, and Pb determination by inductively coupled plasma mass spectrometry (ICP-MS). Sample mass (up to 1.0 g), absorbing solution (0.5 to 14.4 mol L-1 HNO3, and H2O), heating program, and combustion aids were evaluated. The Eurachem guidelines were used for method validation. The proposed method enabled combustion of a high sample mass (0.8 g of honey, with 0.4 g of microcrystalline cellulose and 100 µL of 6 mol L-1 NH4NO3) usi...
Source: Analytical and Bioanalytical Chemistry - March 9, 2024 Category: Chemistry Authors: Vitoria H Cauduro Camila M A C Alves Mariele S Nascimento Gabriel T Druzian Fernanda P Balbinot Marcia F Mesko Erico M M Flores Source Type: research
Determining pyroxasulfone herbicide in honey samples using liquid-liquid extraction with low temperature purification (LLE-LTP)
Talanta. 2024 Mar 2;273:125870. doi: 10.1016/j.talanta.2024.125870. Online ahead of print.ABSTRACTPyroxasulfone is a selective, systemic, pre-emergence herbicide which acts to inhibit weeds in potato, coffee, sugar cane, eucalyptus, and soybean plantations, among others. This active ingredient was classified by Brazilian legislation as a very dangerous product for the environment, and to date there are no studies involving the development of extraction methods for monitoring this compound in environmental matrices. Therefore, the objective of this study was to optimize and validate liquid-liquid extraction with low tempera...
Source: Talanta - March 9, 2024 Category: Chemistry Authors: Luma Soares Costa Carolina Freitas Schettino L ázaro Chaves Sicupira Gevany Paulino de Pinho Flaviano Oliveira Silv ério Source Type: research
Varroa destructor parasitism and Deformed wing virus infection in honey bees are linked to peroxisome ‐induced pathways
This study complements previous research with different study designs and suggests the importance of the peroxisome, which plays a key role in viral infections. (Source: Proteomics)
Source: Proteomics - March 7, 2024 Category: Biochemistry Authors: Tomas Erban,
Dominika Kadleckova,
Bruno Sopko,
Karel Harant,
Pavel Talacko,
Martin Markovic,
Martina Salakova,
Klara Kadlikova,
Ruth Tachezy,
Jan Tachezy Tags: RESEARCH ARTICLE Source Type: research
Clustering honey samples with unsupervised machine learning methods using FTIR data
This study utilizes Fourier transform infrared (FTIR) data from honey samples to cluster and categorize them based on their spectral characteristics. The aim is to group similar samples together, revealing patterns and aiding in classification. The process begins by determining the number of clusters using the elbow method, resulting in five distinct clusters. Principal Component Analysis (PCA) is then applied to reduce the dataset's dimensionality by capturing its significant variances. Hierarchical Cluster Analysis (HCA) further refines the sample clusters. 20% of the data, representing identified clusters, is randomly s...
Source: An Acad Bras Cienc - March 7, 2024 Category: Science Authors: Fatih Mehmet Avcu Source Type: research
Exposure of honey bees to mixtures of microbial biopesticides and their effects on bee survival under laboratory conditions
Environ Sci Pollut Res Int. 2024 Mar 7. doi: 10.1007/s11356-024-32753-9. Online ahead of print.ABSTRACTBiopesticides, having as active ingredients viruses, bacteria, or fungi, are developed to substitute or reduce the use of chemical plant protection products in different agrosystems. Though the application of mixtures containing several products is a common practice, interactions between microbial biopesticides and related effects on bees as non-target organisms have not been studied yet. In the current study, we exposed winter bees to five different microbial-based products and their combinations at the maximum recommend...
Source: Environmental Science and Pollution Research International - March 7, 2024 Category: Environmental Health Authors: Abdulrahim T Alkassab Silvio Erler Michael Steinert Jens Pistorius Source Type: research
Clustering honey samples with unsupervised machine learning methods using FTIR data
This study utilizes Fourier transform infrared (FTIR) data from honey samples to cluster and categorize them based on their spectral characteristics. The aim is to group similar samples together, revealing patterns and aiding in classification. The process begins by determining the number of clusters using the elbow method, resulting in five distinct clusters. Principal Component Analysis (PCA) is then applied to reduce the dataset's dimensionality by capturing its significant variances. Hierarchical Cluster Analysis (HCA) further refines the sample clusters. 20% of the data, representing identified clusters, is randomly s...
Source: An Acad Bras Cienc - March 7, 2024 Category: Science Authors: Fatih Mehmet Avcu Source Type: research
Clustering honey samples with unsupervised machine learning methods using FTIR data
This study utilizes Fourier transform infrared (FTIR) data from honey samples to cluster and categorize them based on their spectral characteristics. The aim is to group similar samples together, revealing patterns and aiding in classification. The process begins by determining the number of clusters using the elbow method, resulting in five distinct clusters. Principal Component Analysis (PCA) is then applied to reduce the dataset's dimensionality by capturing its significant variances. Hierarchical Cluster Analysis (HCA) further refines the sample clusters. 20% of the data, representing identified clusters, is randomly s...
Source: An Acad Bras Cienc - March 7, 2024 Category: Science Authors: Fatih Mehmet Avcu Source Type: research
