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Performance of Field's Stain Compared with Conventional Giemsa Stain for the Rapid Detection of Blood Microfilariae in Gabon
Am J Trop Med Hyg. 2022 Jul 5:tpmd220061. doi: 10.4269/ajtmh.22-0061. Online ahead of print.ABSTRACTFilarial infections caused by Loa loa and Mansonella perstans are a considerable public health burden in rural regions of Central Africa. Rapid diagnostic tools for the detection of microfilariae in the blood are needed. Field's stain is a rapid staining technique for microscopic slides originally established for malaria diagnostics. It requires less than 1 minute of staining compared with conventional staining protocols requiring at least 15 to 20 minutes for staining and could thus significantly accelerate diagnostics for ...
Source: The American Journal of Tropical Medicine and Hygiene - July 27, 2022 Category: Tropical Medicine Authors: Franck-A Ekoka Mbassi Ghyslain Mombo-Ngoma Wilfrid Ndzebe Ndoumba Emmanuel K Yovo Kirsten A Eberhardt Dorothea Ekoka Mbassi Ay ôla A Adegnika Selidji T Agnandji Marielle K Bouyou-Akotet Michael Ramharter Rella Zoleko-Manego Source Type: research

Performance of Field's Stain Compared with Conventional Giemsa Stain for the Rapid Detection of Blood Microfilariae in Gabon
Am J Trop Med Hyg. 2022 Jul 5:tpmd220061. doi: 10.4269/ajtmh.22-0061. Online ahead of print.ABSTRACTFilarial infections caused by Loa loa and Mansonella perstans are a considerable public health burden in rural regions of Central Africa. Rapid diagnostic tools for the detection of microfilariae in the blood are needed. Field's stain is a rapid staining technique for microscopic slides originally established for malaria diagnostics. It requires less than 1 minute of staining compared with conventional staining protocols requiring at least 15 to 20 minutes for staining and could thus significantly accelerate diagnostics for ...
Source: Am J Trop Med Hyg - July 27, 2022 Category: Infectious Diseases Authors: Franck-A Ekoka Mbassi Ghyslain Mombo-Ngoma Wilfrid Ndzebe Ndoumba Emmanuel K Yovo Kirsten A Eberhardt Dorothea Ekoka Mbassi Ay ôla A Adegnika Selidji T Agnandji Marielle K Bouyou-Akotet Michael Ramharter Rella Zoleko-Manego Source Type: research

An Adaptable Platform for in-house Hepatitis C Serology
This study provides a thorough description of the development, and validation of a multiepitope ELISA-based diagnostic assay against HCV, which could be implemented at low cost. The described methodology can be readily adapted to develop novel ELISA-based diagnostic assays for other infectious pathogens with well-described immunogenic epitopes. This method could improve the diagnosis of neglected diseases for which affordable diagnostic assays are lacking.PMID:35850366 | DOI:10.1016/j.jviromet.2022.114586 (Source: Journal of Virological Methods)
Source: Journal of Virological Methods - July 19, 2022 Category: Virology Authors: Jannie Pedersen Ir ène Pegha Moukandja Stella Ndidi Anna-Louise S ørensen Isma ël Hervé Koumakpayi Jean-Bernard Lekana-Douki Marie-Louise Vachon Nina Weis Gary Kobinger Hugues Fausther-Bovendo Source Type: research

Can former conservation ‘pirates’ help scientists study the oceans?
Laura Sánchez Alòs had been at sea for less than 20 minutes when the motorboats attacked. In late January 2019, the 24-year-old Spanish conservation biologist was on board the Farley Mowat , a former U.S. Coast Guard cutter, in Mexican waters in the northern Gulf of California. She was the ship’s scientist, part of a campaign by the Sea Shepherd Conservation Society to study and protect the vaquita , the world’s smallest porpoise and most endangered marine mammal. Since 2015, the organization had been patrolling this region—and making local fishers increasingly angry. Sánchez Alòs...
Source: Science of Aging Knowledge Environment - June 30, 2022 Category: Geriatrics Source Type: research