Genes, Vol. 15, Pages 303: Autotetraploid Induction of Three A-Genome Wild Peanut Species, Arachis cardenasii, A. correntina, and A. diogoi
Hsuan Chen A-genome Arachis species (AA; 2n = 2x = 20) are commonly used as secondary germplasm sources in cultivated peanut breeding, Arachis hypogaea L. (AABB; 2n = 4x = 40), for the introgression of various biotic and abiotic stress resistance genes. Genome doubling is critical to overcoming the hybridization barrier of infertility that arises from ploidy-level differences between wild germplasm and cultivated peanuts. To develop improved genome doubling methods, four trials of various concentrations of the mitotic inhibitor treatments colchicine, oryzalin, and trifluralin were tested on the seedlings and seeds of ...
Source: Genes - February 27, 2024 Category: Genetics & Stem Cells Authors: Robert W. Suppa Ryan J. Andres Jeffrey C. Dunne Ramsey F. Arram Thomas B. Morgan Hsuan Chen Tags: Article Source Type: research

Fucoidan-induced reduction of lipid accumulation in foam cells through overexpression of lysosome genes
In this study, three inhibitors, CPZ, EIPA, and colchicine, were used to inhibit endocytosis, macropinocytosis, and the tubulin pathway, respectively, to study the pathways of action. Transcriptomics and proteomics analysis, as well as western blotting and qRT-PCR were used to determine the effects of fucoidan and the inhibitors on lysosomal genes and proteins. Fucoidan could enter foam cells through both endocytosis and via macropinocytosis, and then further undergo intracellular transport via the tubulin pathway. After fucoidan treatment, the expression of lysosomal pathway-related genes and proteins including LAMP2, AP3...
Source: International Journal of Biological Macromolecules - February 26, 2024 Category: Biochemistry Authors: Shuliang Song Yan Wang Hongming Wang Xiao Tian Xiao Zhang Qian Zhang Qiang Wei Kai Ji Source Type: research

Fucoidan-induced reduction of lipid accumulation in foam cells through overexpression of lysosome genes
In this study, three inhibitors, CPZ, EIPA, and colchicine, were used to inhibit endocytosis, macropinocytosis, and the tubulin pathway, respectively, to study the pathways of action. Transcriptomics and proteomics analysis, as well as western blotting and qRT-PCR were used to determine the effects of fucoidan and the inhibitors on lysosomal genes and proteins. Fucoidan could enter foam cells through both endocytosis and via macropinocytosis, and then further undergo intracellular transport via the tubulin pathway. After fucoidan treatment, the expression of lysosomal pathway-related genes and proteins including LAMP2, AP3...
Source: Atherosclerosis - February 26, 2024 Category: Cardiology Authors: Shuliang Song Yan Wang Hongming Wang Xiao Tian Xiao Zhang Qian Zhang Qiang Wei Kai Ji Source Type: research

Effects of colchicine on the prevention of AF recurrence after atrial ablation: a systematic review and meta-analysis
ConclusionProphylactic use of colchicine after atrial ablation was not associated with a reduction in AF recurrence and pericarditis rates. In addition, there was no difference in the rate of all-cause hospitalization between the groups, and colchicine use was associated with gastrointestinal adverse events.Graphical Abstract (Source: Journal of Interventional Cardiac Electrophysiology)
Source: Journal of Interventional Cardiac Electrophysiology - February 24, 2024 Category: Cardiology Source Type: research

Design, synthesis and biological evaluation of novel podophyllotoxin derivatives as tubulin-targeting anticancer agents
CONCLUSIONS: This research lays the groundwork for advancing cancer treatment through developing and using PPT derivatives. The encouraging results associated with E5 call for extended research and clinical validation, leading to novel and more effective cancer therapies.PMID:38393642 | DOI:10.1080/13880209.2024.2318350 (Source: Cancer Control)
Source: Cancer Control - February 23, 2024 Category: Cancer & Oncology Authors: Yujin Guo Beibei Chen Jinxiu Guo Pei Jiang Jianhua Wang Wenxue Sun Source Type: research

Design, synthesis and biological evaluation of novel podophyllotoxin derivatives as tubulin-targeting anticancer agents
CONCLUSIONS: This research lays the groundwork for advancing cancer treatment through developing and using PPT derivatives. The encouraging results associated with E5 call for extended research and clinical validation, leading to novel and more effective cancer therapies.PMID:38393642 | DOI:10.1080/13880209.2024.2318350 (Source: Pharmaceutical Biology)
Source: Pharmaceutical Biology - February 23, 2024 Category: Drugs & Pharmacology Authors: Yujin Guo Beibei Chen Jinxiu Guo Pei Jiang Jianhua Wang Wenxue Sun Source Type: research

Serum endocan, asymmetric dimethylarginine and lipid profile in children with familial Mediterranean fever
CONCLUSION: Serum ADMA and lipid profile might be used as potential markers for endothelial dysfunction and increased cardiovascular risk in FMF patients.IMPACT: Theoretically, serum ADMA may affect lipid profiles and serum endocan represents an intriguing biomarker related to inflammation. Coexistence of dyslipidemia represents an additional risk factor that contributes to the onset of early atherosclerosis. A few studies investigated the role of changes in lipid profile and lipid ratios in accelerated atherosclerosis pathogenesis in FMF patients. The relationship between colchicine and lipid profile is contradictory. Alt...
Source: Atherosclerosis - February 23, 2024 Category: Cardiology Authors: Rania S El Zayat Fahima M Hassan Noran T Aboelkhair Walaa F Abdelhakeem Ahmed S Abo Hola Source Type: research

Serum endocan, asymmetric dimethylarginine and lipid profile in children with familial Mediterranean fever
CONCLUSION: Serum ADMA and lipid profile might be used as potential markers for endothelial dysfunction and increased cardiovascular risk in FMF patients.IMPACT: Theoretically, serum ADMA may affect lipid profiles and serum endocan represents an intriguing biomarker related to inflammation. Coexistence of dyslipidemia represents an additional risk factor that contributes to the onset of early atherosclerosis. A few studies investigated the role of changes in lipid profile and lipid ratios in accelerated atherosclerosis pathogenesis in FMF patients. The relationship between colchicine and lipid profile is contradictory. Alt...
Source: Pediatric Research - February 23, 2024 Category: Pediatrics Authors: Rania S El Zayat Fahima M Hassan Noran T Aboelkhair Walaa F Abdelhakeem Ahmed S Abo Hola Source Type: research

Design, synthesis and biological evaluation of novel podophyllotoxin derivatives as tubulin-targeting anticancer agents
CONCLUSIONS: This research lays the groundwork for advancing cancer treatment through developing and using PPT derivatives. The encouraging results associated with E5 call for extended research and clinical validation, leading to novel and more effective cancer therapies.PMID:38393642 | DOI:10.1080/13880209.2024.2318350 (Source: Pharmaceutical Biology)
Source: Pharmaceutical Biology - February 23, 2024 Category: Drugs & Pharmacology Authors: Yujin Guo Beibei Chen Jinxiu Guo Pei Jiang Jianhua Wang Wenxue Sun Source Type: research

Design, synthesis and biological evaluation of novel podophyllotoxin derivatives as tubulin-targeting anticancer agents
CONCLUSIONS: This research lays the groundwork for advancing cancer treatment through developing and using PPT derivatives. The encouraging results associated with E5 call for extended research and clinical validation, leading to novel and more effective cancer therapies.PMID:38393642 | PMC:PMC10896134 | DOI:10.1080/13880209.2024.2318350 (Source: Pharmaceutical Biology)
Source: Pharmaceutical Biology - February 23, 2024 Category: Drugs & Pharmacology Authors: Yujin Guo Beibei Chen Jinxiu Guo Pei Jiang Jianhua Wang Wenxue Sun Source Type: research

Cluster of Differentiation-44-Targeting Prussian Blue Nanoparticles Onloaded with Colchicine for Atherosclerotic Plaque Regression in a Mice Model
In this study, the cluster of differentiation-44 receptor was verified to be overexpressed in inflammatory macrophages within plaques both in vitro and in vivo. Subsequently, a Prussian blue-based nanomedical loading system with hyaluronic acid (HA) coating was constructed, and its effects were observed on the atherosclerosis regression. Colchicine and Cy5.5 were encapsulated within Prussian blue nanoparticles through self-assembly, followed by conjugation with hyaluronic acid to create col@PBNP@HA. The formulated col@PBNP@HA displayed a cubic shape and scattered distribution. Importantly, col@PBNP@HA demonstrated specific...
Source: Atherosclerosis - February 19, 2024 Category: Cardiology Authors: Yan Zhu Yan Fang Yujia Wang Dong Han Jing Liu Lei Tian Mengqi Xu Yabin Wang Feng Cao Source Type: research

Mesenteric artery smooth muscle cells from hypertensive rats have increased microtubule acetylation
Conclusions:</p> Acetylation of tubulin at Lys40 is elevated in mesenteric arteries from the SHR. Furthermore, this study shows that tubacin has an endothelial-dependent bimodal effect on isoprenaline-mediated vasorelaxation.PMID:38373073 | DOI:10.1042/BCJ20230420 (Source: The Biochemical Journal)
Source: The Biochemical Journal - February 19, 2024 Category: Biochemistry Authors: Anthony M Mozzicato Joakim A Bastrup Jose L Sanchez Alonso Jennifer van der Horst Julia Gorelik Per M årten Hägglund Thomas Jepps Source Type: research

Cluster of Differentiation-44-Targeting Prussian Blue Nanoparticles Onloaded with Colchicine for Atherosclerotic Plaque Regression in a Mice Model
In this study, the cluster of differentiation-44 receptor was verified to be overexpressed in inflammatory macrophages within plaques both in vitro and in vivo. Subsequently, a Prussian blue-based nanomedical loading system with hyaluronic acid (HA) coating was constructed, and its effects were observed on the atherosclerosis regression. Colchicine and Cy5.5 were encapsulated within Prussian blue nanoparticles through self-assembly, followed by conjugation with hyaluronic acid to create col@PBNP@HA. The formulated col@PBNP@HA displayed a cubic shape and scattered distribution. Importantly, col@PBNP@HA demonstrated specific...
Source: Atherosclerosis - February 19, 2024 Category: Cardiology Authors: Yan Zhu Yan Fang Yujia Wang Dong Han Jing Liu Lei Tian Mengqi Xu Yabin Wang Feng Cao Source Type: research