Echinocandin Resistance in Candida auris Occurs in the Murine Gastrointestinal Tract Due to < em > FKS1 < /em > Mutations
This study investigated its antifungal susceptibility and explored FKS1 mutations across the isolates from mice enterically colonized with wild-type C. auris and treated with echinocandin. Resistant C. auris with FKS1 mutations, including S639F, S639Y, D642Y, R1354H, or R1354Y, were isolated and found to be micafungin- and caspofungin-resistant in vivo; however, the MICs of isolates with mutation in R1354 remained below the micafungin breakpoint in vitro.PMID:36920237 | DOI:10.1128/aac.01243-22 (Source: Antimicrobial Agents and Chemotherapy)
Source: Antimicrobial Agents and Chemotherapy - March 15, 2023 Category: Microbiology Authors: Tatsuro Hirayama Taiga Miyazaki Makoto Sumiyoshi Yuya Ito Nobuyuki Ashizawa Kazuaki Takeda Naoki Iwanaga Takahiro Takazono Kazuko Yamamoto Koichi Izumikawa Katsunori Yanagihara Koichi Makimura Kazuhiro Tsukamoto Shigeru Kohno Hiroshi Mukae Source Type: research
PK/PD modeling and simulation of the in vitro activity of the combinations of isavuconazole with echinocandins against Candida auris
AbstractIn vitro combination of echinocandins and isavuconazole against the emerging speciesCandida auris is mainly synergistic. However, this combination has not been evaluated in clinical settings. A pharmacokinetic/pharmacodynamic modeling and simulation approach based on in vitro data may be helpful to further study the therapeutic potential of these combinations. Therefore, the aims of this study were to characterize the time course of growth and killing ofC. auris in response to the combination of the three approved echinocandins and isavuconazole using a semimechanistic model and to perform model-based simulations i...
Source: CPT: Pharmacometrics and Systems Pharmacology - March 14, 2023 Category: Drugs & Pharmacology Authors: Unai Caballero,
Elena Eraso,
Guillermo Quind ós,
Valvanera Vozmediano,
Stephan Schmidt,
Nerea Jauregizar Tags: ARTICLE Source Type: research
A cell wall-targeted organic-inorganic hybrid nano-catcher for ultrafast capture and SERS detection of invasive fungi
Biosens Bioelectron. 2023 Feb 23;228:115173. doi: 10.1016/j.bios.2023.115173. Online ahead of print.ABSTRACTDue to the extended culture period and various inconveniences in vitro culture, the detection of invasive fungi is rather difficult, leading to high mortality rates of the diseases caused by them. It is, however, crucial for clinical therapy and lowering patient mortality to quickly identify invasive fungus from clinical specimens. A promising non-destructive method for finding fungi is surface-enhanced Raman scattering (SERS), however, its substrate has a low level of selectivity. Clinical sample components can obst...
Source: Biosensors and Bioelectronics - March 6, 2023 Category: Biotechnology Authors: Yu Xu Feng Gu Shan Hu Yunjian Wu Changyu Wu Yaling Deng Bing Gu Zheng Chen Ying Yang Source Type: research
A cell wall-targeted organic-inorganic hybrid nano-catcher for ultrafast capture and SERS detection of invasive fungi
Biosens Bioelectron. 2023 Feb 23;228:115173. doi: 10.1016/j.bios.2023.115173. Online ahead of print.ABSTRACTDue to the extended culture period and various inconveniences in vitro culture, the detection of invasive fungi is rather difficult, leading to high mortality rates of the diseases caused by them. It is, however, crucial for clinical therapy and lowering patient mortality to quickly identify invasive fungus from clinical specimens. A promising non-destructive method for finding fungi is surface-enhanced Raman scattering (SERS), however, its substrate has a low level of selectivity. Clinical sample components can obst...
Source: Biosensors and Bioelectronics - March 6, 2023 Category: Biotechnology Authors: Yu Xu Feng Gu Shan Hu Yunjian Wu Changyu Wu Yaling Deng Bing Gu Zheng Chen Ying Yang Source Type: research
A cell wall-targeted organic-inorganic hybrid nano-catcher for ultrafast capture and SERS detection of invasive fungi
Biosens Bioelectron. 2023 Feb 23;228:115173. doi: 10.1016/j.bios.2023.115173. Online ahead of print.ABSTRACTDue to the extended culture period and various inconveniences in vitro culture, the detection of invasive fungi is rather difficult, leading to high mortality rates of the diseases caused by them. It is, however, crucial for clinical therapy and lowering patient mortality to quickly identify invasive fungus from clinical specimens. A promising non-destructive method for finding fungi is surface-enhanced Raman scattering (SERS), however, its substrate has a low level of selectivity. Clinical sample components can obst...
Source: Biosensors and Bioelectronics - March 6, 2023 Category: Biotechnology Authors: Yu Xu Feng Gu Shan Hu Yunjian Wu Changyu Wu Yaling Deng Bing Gu Zheng Chen Ying Yang Source Type: research
A SARS-CoV-2-specific CAR-T-cell model identifies felodipine, fasudil, imatinib, and caspofungin as potential treatments for lethal COVID-19
Cell Mol Immunol. 2023 Mar 2:1-14. doi: 10.1038/s41423-023-00985-3. Online ahead of print.ABSTRACTSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced cytokine storm is closely associated with coronavirus disease 2019 (COVID-19) severity and lethality. However, drugs that are effective against inflammation to treat lethal COVID-19 are still urgently needed. Here, we constructed a SARS-CoV-2 spike protein-specific CAR, and human T cells infected with this CAR (SARS-CoV-2-S CAR-T) and stimulated with spike protein mimicked the T-cell responses seen in COVID-19 patients, causing cytokine storm and displaying a...
Source: Cellular and Molecular Immunology - March 2, 2023 Category: Molecular Biology Authors: Lin Xia Lun-Zhi Yuan Ya-Hong Hu Jun-Yi Liu Guo-Sheng Hu Ruo-Yao Qi Tian-Ying Zhang Hua-Long Xiong Zao-Zao Zheng Hong-Wei Lin Jia-Mo Zhang Chao Yu Ming Zhou Jian Ma Tong Cheng Ri-Rong Chen Yi Guan Ning-Shao Xia Wen Liu Source Type: research
A SARS-CoV-2-specific CAR-T-cell model identifies felodipine, fasudil, imatinib, and caspofungin as potential treatments for lethal COVID-19
Cell Mol Immunol. 2023 Mar 2:1-14. doi: 10.1038/s41423-023-00985-3. Online ahead of print.ABSTRACTSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced cytokine storm is closely associated with coronavirus disease 2019 (COVID-19) severity and lethality. However, drugs that are effective against inflammation to treat lethal COVID-19 are still urgently needed. Here, we constructed a SARS-CoV-2 spike protein-specific CAR, and human T cells infected with this CAR (SARS-CoV-2-S CAR-T) and stimulated with spike protein mimicked the T-cell responses seen in COVID-19 patients, causing cytokine storm and displaying a...
Source: Cellular and Molecular Immunology - March 2, 2023 Category: Molecular Biology Authors: Lin Xia Lun-Zhi Yuan Ya-Hong Hu Jun-Yi Liu Guo-Sheng Hu Ruo-Yao Qi Tian-Ying Zhang Hua-Long Xiong Zao-Zao Zheng Hong-Wei Lin Jia-Mo Zhang Chao Yu Ming Zhou Jian Ma Tong Cheng Ri-Rong Chen Yi Guan Ning-Shao Xia Wen Liu Source Type: research
AFM evaluation of a humanized recombinant antibody affecting < em > C. auris < /em > cell wall and stability
RSC Adv. 2023 Feb 20;13(9):6130-6142. doi: 10.1039/d2ra07217c. eCollection 2023 Feb 14.ABSTRACTFungal infections are increasingly impacting on the health of the population and particularly on subjects with a compromised immune system. The resistance phenomenon and the rise of new species carrying sometimes intrinsic and multi-drug resistance to the most commonly used antifungal drugs are greatly concerning healthcare organizations. As a result of this situation, there is growing interest in the development of therapeutic agents against pathogenic fungi. In particular, the Candida genus is responsible for severe life-threat...
Source: Adv Data - February 23, 2023 Category: Epidemiology Authors: Tania Vanzolini Tomas Di Mambro Mauro Magnani Michele Menotta Source Type: research
Viruses, Vol. 15, Pages 452: Antiviral Activity of Micafungin and Its Derivatives against SARS-CoV-2 RNA Replication
In this study, we focused on micafungin and its derivatives and analyzed their antiviral activities against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The micafungin derivatives Mi-2 and Mi-5 showed higher antiviral activity than micafungin, with 50% maximal inhibitory concentration (IC50) of 5.25 and 6.51 &micro;M, respectively (3.8 to 4.7-fold stronger than micafungin) and 50% cytotoxic concentration (CC50) of &gt;64 &micro;M in VeroE6/TMPRSS2 cells. This high anti-SARS-CoV-2 activity was also conserved in human lung epithelial cell-derived Calu-3 cells. Micafungin, Mi-2, and Mi...
Source: Viruses - February 6, 2023 Category: Virology Authors: Shogo Nakajima Hirofumi Ohashi Daisuke Akazawa Shiho Torii Rigel Suzuki Takasuke Fukuhara Koichi Watashi Tags: Brief Report Source Type: research
A six-year hospital-based surveillance study on burden of esophageal candidiasis in Gangtok, Sikkim
CONCLUSION: Upper gastrointestinal symptoms even in non-immunocompromised patients need to be screened by endoscopy to rule out esophageal candidiasis. With the emergence of drug resistant non albicans Candida species diagnostic testing laboratories should include Candida species identification and antifungal susceptibility testing facility to provide effective patient care.PMID:36721503 | PMC:PMC9867645 | DOI:10.18502/ijm.v14i4.10247 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - February 1, 2023 Category: Microbiology Authors: Shrijana Gurung Tara Devi Sharma Suresh Madan Rasaily Raju Singh Peralam Yegneswaran Prakash Source Type: research
Saprochaete clavata invasive infection: characterization, antifungal susceptibility, and biofilm evaluation of a rare yeast isolated in Brazil
The objective of this study was to describe a new case of severe S. clavata infection in a patient diagnosed at a referral children's hospital in Brazil, including antifungal minimal inhibitory concentration, S. clavata biofilm characterization, and molecular characterization. The S. clavata isolated from an immunocompromised 11-year-old male patient was characterized using MALDI-TOF, Gram staining, scanning electron microscopy (SEM), and next generation sequencing (NGS) of genomic DNA. Biofilm production was also evaluated in parallel with determining minimal inhibitory concentration (MIC) and biofilm sensitivity to antif...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 1, 2023 Category: Tropical Medicine Authors: Let ícia Kraft Victoria Stadler Tasca Ribeiro Luiz Pedro Petroski Roberto Hirochi Herai Kamila Chagas Peronni David Livingstone Alves Figueiredo F ábio Araujo Motta Felipe Francisco Tuon Source Type: research
A six-year hospital-based surveillance study on burden of esophageal candidiasis in Gangtok, Sikkim
CONCLUSION: Upper gastrointestinal symptoms even in non-immunocompromised patients need to be screened by endoscopy to rule out esophageal candidiasis. With the emergence of drug resistant non albicans Candida species diagnostic testing laboratories should include Candida species identification and antifungal susceptibility testing facility to provide effective patient care.PMID:36721503 | PMC:PMC9867645 | DOI:10.18502/ijm.v14i4.10247 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - February 1, 2023 Category: Microbiology Authors: Shrijana Gurung Tara Devi Sharma Suresh Madan Rasaily Raju Singh Peralam Yegneswaran Prakash Source Type: research
Saprochaete clavata invasive infection: characterization, antifungal susceptibility, and biofilm evaluation of a rare yeast isolated in Brazil
The objective of this study was to describe a new case of severe S. clavata infection in a patient diagnosed at a referral children's hospital in Brazil, including antifungal minimal inhibitory concentration, S. clavata biofilm characterization, and molecular characterization. The S. clavata isolated from an immunocompromised 11-year-old male patient was characterized using MALDI-TOF, Gram staining, scanning electron microscopy (SEM), and next generation sequencing (NGS) of genomic DNA. Biofilm production was also evaluated in parallel with determining minimal inhibitory concentration (MIC) and biofilm sensitivity to antif...
Source: Revista do Instituto de Medicina Tropical de Sao Paulo - February 1, 2023 Category: Tropical Medicine Authors: Let ícia Kraft Victoria Stadler Tasca Ribeiro Luiz Pedro Petroski Roberto Hirochi Herai Kamila Chagas Peronni David Livingstone Alves Figueiredo F ábio Araujo Motta Felipe Francisco Tuon Source Type: research
A six-year hospital-based surveillance study on burden of esophageal candidiasis in Gangtok, Sikkim
CONCLUSION: Upper gastrointestinal symptoms even in non-immunocompromised patients need to be screened by endoscopy to rule out esophageal candidiasis. With the emergence of drug resistant non albicans Candida species diagnostic testing laboratories should include Candida species identification and antifungal susceptibility testing facility to provide effective patient care.PMID:36721503 | PMC:PMC9867645 | DOI:10.18502/ijm.v14i4.10247 (Source: Iranian Journal of Microbiology)
Source: Iranian Journal of Microbiology - February 1, 2023 Category: Microbiology Authors: Shrijana Gurung Tara Devi Sharma Suresh Madan Rasaily Raju Singh Peralam Yegneswaran Prakash Source Type: research
