Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
Genes, Vol. 14, Pages 962: Alternative Splicing of NAC Transcription Factor Gene CmNST1 Is Associated with Naked Seed Mutation in Pumpkin, Cucurbita moschata
In this study, we report genetic mapping, identification, and characterization of a candidate gene for this mutation. We showed that the naked seed phenotype is controlled by a single recessive gene (N). The bulked segregant analysis identified a 2.4 Mb region on Chromosome 17 with 15 predicted genes. Multiple lines of evidence suggested that CmoCh17G004790 is the most probable candidate gene for the N locus which encodes a NAC transcription factor WALL THICKENING PROMOTING FACTOR 1 (CmNST1). No nucleotide polymorphism or structural variation was found in the genomic DNA sequences of CmNST1 between the mutant and the wildt...
Source: Genes - April 23, 2023 Category: Genetics & Stem Cells Authors: Qiong Shen Yiqun Weng Tags: Article Source Type: research
Pro ‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts
Int J Mol Med. 2023 May;51(5):39. doi: 10.3892/ijmm.2023.5242. Epub 2023 Apr 7.ABSTRACTIntervertebral disc (IVD) degeneration (IDD) is closely associated with inflammation, oxidative stress and loss of the discogenic phenotype, which current therapies are unable to reverse. In the present study, the effects of acetone extract from Violina pumpkin (Cucurbita moschata) leaves on degenerated IVD cells were investigated. IVD cells were isolated from the degenerated disc tissue of patients undergoing spinal surgery and were exposed to acetone extract and three major thin layer chromatography subfractions. The results revealed t...
Source: International Journal of Molecular Medicine - April 7, 2023 Category: Molecular Biology Authors: Elisabetta Lambertini Letizia Penolazzi Maria Pina Notarangelo Serena Fiorito Francesco Epifano Assunta Pandolfi Roberta Piva Source Type: research
Pro ‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts
Int J Mol Med. 2023 May;51(5):39. doi: 10.3892/ijmm.2023.5242. Epub 2023 Apr 7.ABSTRACTIntervertebral disc (IVD) degeneration (IDD) is closely associated with inflammation, oxidative stress and loss of the discogenic phenotype, which current therapies are unable to reverse. In the present study, the effects of acetone extract from Violina pumpkin (Cucurbita moschata) leaves on degenerated IVD cells were investigated. IVD cells were isolated from the degenerated disc tissue of patients undergoing spinal surgery and were exposed to acetone extract and three major thin layer chromatography subfractions. The results revealed t...
Source: Molecular Medicine - April 7, 2023 Category: Molecular Biology Authors: Elisabetta Lambertini Letizia Penolazzi Maria Pina Notarangelo Serena Fiorito Francesco Epifano Assunta Pandolfi Roberta Piva Source Type: research
Pro ‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts
Int J Mol Med. 2023 May;51(5):39. doi: 10.3892/ijmm.2023.5242. Epub 2023 Apr 7.ABSTRACTIntervertebral disc (IVD) degeneration (IDD) is closely associated with inflammation, oxidative stress and loss of the discogenic phenotype, which current therapies are unable to reverse. In the present study, the effects of acetone extract from Violina pumpkin (Cucurbita moschata) leaves on degenerated IVD cells were investigated. IVD cells were isolated from the degenerated disc tissue of patients undergoing spinal surgery and were exposed to acetone extract and three major thin layer chromatography subfractions. The results revealed t...
Source: International Journal of Molecular Medicine - April 7, 2023 Category: Molecular Biology Authors: Elisabetta Lambertini Letizia Penolazzi Maria Pina Notarangelo Serena Fiorito Francesco Epifano Assunta Pandolfi Roberta Piva Source Type: research
Pro ‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts
Int J Mol Med. 2023 May;51(5):39. doi: 10.3892/ijmm.2023.5242. Epub 2023 Apr 7.ABSTRACTIntervertebral disc (IVD) degeneration (IDD) is closely associated with inflammation, oxidative stress and loss of the discogenic phenotype, which current therapies are unable to reverse. In the present study, the effects of acetone extract from Violina pumpkin (Cucurbita moschata) leaves on degenerated IVD cells were investigated. IVD cells were isolated from the degenerated disc tissue of patients undergoing spinal surgery and were exposed to acetone extract and three major thin layer chromatography subfractions. The results revealed t...
Source: International Journal of Molecular Medicine - April 7, 2023 Category: Molecular Biology Authors: Elisabetta Lambertini Letizia Penolazzi Maria Pina Notarangelo Serena Fiorito Francesco Epifano Assunta Pandolfi Roberta Piva Source Type: research
Pro ‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts
Int J Mol Med. 2023 May;51(5):39. doi: 10.3892/ijmm.2023.5242. Epub 2023 Apr 7.ABSTRACTIntervertebral disc (IVD) degeneration (IDD) is closely associated with inflammation, oxidative stress and loss of the discogenic phenotype, which current therapies are unable to reverse. In the present study, the effects of acetone extract from Violina pumpkin (Cucurbita moschata) leaves on degenerated IVD cells were investigated. IVD cells were isolated from the degenerated disc tissue of patients undergoing spinal surgery and were exposed to acetone extract and three major thin layer chromatography subfractions. The results revealed t...
Source: International Journal of Molecular Medicine - April 7, 2023 Category: Molecular Biology Authors: Elisabetta Lambertini Letizia Penolazzi Maria Pina Notarangelo Serena Fiorito Francesco Epifano Assunta Pandolfi Roberta Piva Source Type: research
Pro ‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts
Int J Mol Med. 2023 May;51(5):39. doi: 10.3892/ijmm.2023.5242. Epub 2023 Apr 7.ABSTRACTIntervertebral disc (IVD) degeneration (IDD) is closely associated with inflammation, oxidative stress and loss of the discogenic phenotype, which current therapies are unable to reverse. In the present study, the effects of acetone extract from Violina pumpkin (Cucurbita moschata) leaves on degenerated IVD cells were investigated. IVD cells were isolated from the degenerated disc tissue of patients undergoing spinal surgery and were exposed to acetone extract and three major thin layer chromatography subfractions. The results revealed t...
Source: International Journal of Molecular Medicine - April 7, 2023 Category: Molecular Biology Authors: Elisabetta Lambertini Letizia Penolazzi Maria Pina Notarangelo Serena Fiorito Francesco Epifano Assunta Pandolfi Roberta Piva Source Type: research
Effects of different planting distances and fertilizer use on the remediation of farmland contaminated with Cd by intercropping Cucurbita moschata and Amaranthus hypochondriacus L
In this study, the effects of different planting densities and fertilizer applications on the crop growth and Cd absorption of a pumpkin (Cucurbita moschata)-Amaranthus hypochondriacus L. intercropping system was determined. The goal was to provide enhanced means and a scientific basis for the promotion and application of this intercropping system. The Cd content of pumpkin in different planting systems was lower than the national food safety standard (0.05 mg kg-1). In the IN-1 (4 pumpkin plants intercropped with 200 A. hypochondriacus plants) and IN-2 (4 pumpkin plants intercropped with 400 A. hypochondriacus plants) int...
Source: Environmental Science and Pollution Research International - March 1, 2023 Category: Environmental Health Authors: Weizhen Chen Mengya Zhou Yanan Yang Dele Meng Jidong Ying Yinshi Li Zhiming Kang Huashou Li Source Type: research
