Screening and characterization of an anti-inflammatory pectic polysaccharide from Cucurbita moschata Duch
This study aims to extract, characterize and evaluate the effects of pumpkin pectin polysaccharide on lipopolysaccharide (LPS)-induced inflammatory response in RAW264.7 cells and dextran sulfate sodium (DSS)-induced colitis in mice, along with its underlying mechanism of action. Initially, we extracted three fractions of pectin polysaccharides from pumpkin and screened them for anti-inflammatory activity in LPS-induced macrophages, identifying CMDP-3a as the most potent anti-inflammatory fraction. Subsequently, CMDP-3a underwent comprehensive characterization through chromatography and spectroscopic analysis, revealing CMD...
Source: International Journal of Biological Macromolecules - March 6, 2024 Category: Biochemistry Authors: Linlin Huang Qi Sun Quanhong Li Xin Li Source Type: research
Screening and characterization of an anti-inflammatory pectic polysaccharide from Cucurbita moschata Duch
This study aims to extract, characterize and evaluate the effects of pumpkin pectin polysaccharide on lipopolysaccharide (LPS)-induced inflammatory response in RAW264.7 cells and dextran sulfate sodium (DSS)-induced colitis in mice, along with its underlying mechanism of action. Initially, we extracted three fractions of pectin polysaccharides from pumpkin and screened them for anti-inflammatory activity in LPS-induced macrophages, identifying CMDP-3a as the most potent anti-inflammatory fraction. Subsequently, CMDP-3a underwent comprehensive characterization through chromatography and spectroscopic analysis, revealing CMD...
Source: International Journal of Biological Macromolecules - March 6, 2024 Category: Biochemistry Authors: Linlin Huang Qi Sun Quanhong Li Xin Li Source Type: research
Gut microbiota-derived 5-hydroxyindoleacetic acid from pumpkin polysaccharides supplementation alleviates colitis via MAPKs-PPAR γ/NF-κB inhibition
This study preliminarily confirmed its significant anti-UC activity superior to Salicylazosulfapyridine. The average molecular weight of PPs was 3.10 × 105 Da, and PPs mainly comprised Mannose, Rhamnose, Galacturonic acid, Galactosamine, Glucose, and Xylose with molar ratios of 1.58:3.51:34.54:1.00:3.25:3.02. PPs (50, 100 mg/kg) could significantly resist dextran sodium sulfate induced UC on C57BL/6 mice by improving gut microbiota dysbiosis, such as the changes of relative abundance of Bacteroides, Culturomica, Mucispirillum, Escherichia-Shigella, Alistipes and Helicobacter. PPs also reverse the abnormal inflammatory rea...
Source: International Journal of Biological Macromolecules - February 23, 2024 Category: Biochemistry Authors: Minglan Wu Qi Wang Xiaodong Li Songxia Yu Fan Zhao Xia Wu Li Fan Xueling Liu Qingwei Zhao Xuelin He Weifen Li Qiao Zhang Xingjiang Hu Source Type: research
Gut microbiota-derived 5-hydroxyindoleacetic acid from pumpkin polysaccharides supplementation alleviates colitis via MAPKs-PPAR γ/NF-κB inhibition
This study preliminarily confirmed its significant anti-UC activity superior to Salicylazosulfapyridine. The average molecular weight of PPs was 3.10 × 105 Da, and PPs mainly comprised Mannose, Rhamnose, Galacturonic acid, Galactosamine, Glucose, and Xylose with molar ratios of 1.58:3.51:34.54:1.00:3.25:3.02. PPs (50, 100 mg/kg) could significantly resist dextran sodium sulfate induced UC on C57BL/6 mice by improving gut microbiota dysbiosis, such as the changes of relative abundance of Bacteroides, Culturomica, Mucispirillum, Escherichia-Shigella, Alistipes and Helicobacter. PPs also reverse the abnormal inflammatory rea...
Source: International Journal of Biological Macromolecules - February 23, 2024 Category: Biochemistry Authors: Minglan Wu Qi Wang Xiaodong Li Songxia Yu Fan Zhao Xia Wu Li Fan Xueling Liu Qingwei Zhao Xuelin He Weifen Li Qiao Zhang Xingjiang Hu Source Type: research
Molecules, Vol. 29, Pages 923: Biosynthesis, Characterization, and Biomedical Applications of Gold Nanoparticles with Cucurbita moschata Duchesne Ex Poiret Peel Aqueous Extracts
In this study, AuNPs were biosynthesized from Cucurbita moschata fruit peel extracts. Biosynthesized AuNPs exhibited maximum absorbance at a 555 nm wavelength, and XRD analysis indicated that the CM-AuNPs had a particle size of less than 100 nm and a cubic crystal structure. TEM scans revealed that the gold particles exhibited a spherical morphology, with an average size of 18.10 nm. FTIR analysis revealed strong peaks indicating the presence of functional groups involved in the reduction reactions. The surface charge of the biosynthesized AuNPs was determined to be −19.7 mV. The antibacterial and antifungal ...
Source: Molecules - February 20, 2024 Category: Chemistry Authors: U ğur Kaval H ülya Hoşgören Tags: Article Source Type: research
Transcriptomic analysis reveals the molecular basis of photoperiod-regulated sex differentiation in tropical pumpkins (Cucurbita moschata Duch.)
Photoperiod, or the length of the day, has a significant impact on the flowering and sex differentiation of photoperiod-sensitive crops. The “miben” pumpkin (the main type of Cucurbita moschata Duch.) is well-kno... (Source: BMC Plant Biology)
Source: BMC Plant Biology - February 6, 2024 Category: Biology Authors: Shudan Xue, Hexun Huang, Yingchao Xu, Ling Liu, Qitao Meng, Jitong Zhu, Meijiang Zhou, Hu Du, Chunpeng Yao, Qingmin Jin, Chengrong Nie and Yujuan Zhong Tags: Research Source Type: research
Genes, Vol. 14, Pages 1877: A Genome-Wide Identification and Comparative Analysis of the Heavy-Metal-Associated Gene Family in Cucurbitaceae Species and Their Role in Cucurbita pepo under Arsenic Stress
Balagurusamy
The heavy-metal-associated (HMA) proteins are a class of PB1-type ATPases related to the intracellular transport and detoxification of metals. However, due to a lack of information regarding the HMA gene family in the Cucurbitaceae family, a comprehensive genome-wide analysis of the HMA family was performed in ten Cucurbitaceae species: Citrullus amarus, Citrullus colocynthis, Citrullus lanatus, Citrullus mucosospermus, Cucumis melo, Cucumis sativus, Cucurbita maxima, Cucurbita moschata, Cucurbita pepo, and Legenaria siceraria. We identified 103 Cucurbit HMA proteins with various members, ranging from 8 (...
Source: Genes - September 27, 2023 Category: Genetics & Stem Cells Authors: Gerardo Flores-Iga Carlos Lopez-Ortiz Celeste Gracia-Rodriguez Aldo Almeida Padma Nimmakayala Umesh K. Reddy Nagamani Balagurusamy Tags: Article Source Type: research
Impact of pumpkin seeds supplementation on anemia in antenatal mothers at Andhra Pradesh, India
Bioinformation. 2022 Nov 30;18(11):1092-1097. doi: 10.6026/973206300181092. eCollection 2022.ABSTRACTAnemia during pregnancy causes 40% of maternal fatalities in underdeveloped nations, according to statistics from the WHO. Pregnant women should meet the requirements for various nutrients, notably micronutrients, to ensure a better pregnancy outcome in the future. Cucurbita moschata (Pumpkin) seeds contain a variety of compounds, such as m-carboxyphenylalanine, amino butyrate, and citrulline, as well as a number of other amino acids. It has a wide variety of biological activity such as antioxidant, anticancer, anti-inflamm...
Source: Bioinformation - September 11, 2023 Category: Bioinformatics Authors: Swapna Kumari Andugula Veerraju Andugula Mani Vadivel Rajapandian Rajapandian Chaitra Jinka Shyamaladevi Babu Babu A Gouri Shankar Source Type: research
Impact of pumpkin seeds supplementation on anemia in antenatal mothers at Andhra Pradesh, India
Bioinformation. 2022 Nov 30;18(11):1092-1097. doi: 10.6026/973206300181092. eCollection 2022.ABSTRACTAnemia during pregnancy causes 40% of maternal fatalities in underdeveloped nations, according to statistics from the WHO. Pregnant women should meet the requirements for various nutrients, notably micronutrients, to ensure a better pregnancy outcome in the future. Cucurbita moschata (Pumpkin) seeds contain a variety of compounds, such as m-carboxyphenylalanine, amino butyrate, and citrulline, as well as a number of other amino acids. It has a wide variety of biological activity such as antioxidant, anticancer, anti-inflamm...
Source: Bioinformation - September 11, 2023 Category: Bioinformatics Authors: Swapna Kumari Andugula Veerraju Andugula Mani Vadivel Rajapandian Rajapandian Chaitra Jinka Shyamaladevi Babu Babu A Gouri Shankar Source Type: research
Molecules, Vol. 28, Pages 6573: In Vivo, In Vitro and In Silico Study of Cucurbita moschata Flower Extract: A Promising Source of Natural Analgesic, Anti-Inflammatory, and Antibacterial Agents
In conclusion, the hydro-methanolic extract of Cucurbita moschata was found to be rich in various phytochemicals that may be associated with therapeutic potential, and this study supports the traditional use of Cucurbita moschata flowers in the management of inflammation and painful conditions. (Source: Molecules)
Source: Molecules - September 11, 2023 Category: Chemistry Authors: Md. Rabiul Hossain Rashedul Alam Hea-Jong Chung Taslima Akter Eva Mohammed Fazlul Kabir Husnum Mamurat Seong-Tshool Hong Md. Al Hafiz S. M. Moazzem Hossen Tags: Article Source Type: research
A pathogenesis-related protein 1 of Cucurbita moschata responds to powdery mildew infection
Pumpkin (Cucurbita moschata Duch.) productivity is severely hindered by powdery mildew (PM) worldwide. The causative agent of pumpkin PM is Podosphaera xanthii, a biotrophic fungus. Pathogenesis-related protein 1 (PR1) homolog was previously identified from transcriptomic analysis of a PM-resistant pumpkin. Here, we investigated the effects of CmPR1 gene from pumpkin for resistance to PM. Subcellular localization assay revealed that CmPR1 is a cytoplasmic protein in plants. The expression of CmPR1 gene was strongly induced by P. xanthii inoculation at 48 h and exogenous ethylene (ET), jasmonic acid (JA) and NaCl treatment...
Source: Frontiers in Genetics - August 1, 2023 Category: Genetics & Stem Cells Source Type: research
Evaluation of Antidiabetic and Antioxidant Activities of Fruit Pulp Extracts of < em > Cucurbita moschata < /em > Duchesne and < em > Cucurbita maxima < /em > Duchesne
CONCLUSIONS: According to the obtained results, it is indicated that PCMOS and PCMAX possess antidiabetic and antioxidant activities. PCMAX possesses more potent antidiabetic and antioxidant activities than PCMOS. These are probably due to PCMAX providing polysaccharide and total phenolic and flavonoid contents more than PCMOS.PMID:37398913 | PMC:PMC10310460 | DOI:10.1155/2023/1124606 (Source: The Scientific World Journal)
Source: The Scientific World Journal - July 3, 2023 Category: Science Authors: Apinya Suwannapong Chusri Talubmook Wilawan Promprom Source Type: research
Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
Comprehensive analyses of microtubule-associated protein MAP65 family genes in Cucurbitaceae and CsaMAP65s expression profiles in cucumber
In this study, a total of 40 MAP65s were identified from six Cucurbitaceae species (Cucumis sativus L., Citrullus lanatus, Cucumis melo L., Cucurbita moschata, Lagenaria siceraria, and Benincasa hispida) and classified into five groups by phylogenetic analysis according to gene structures and conserved domains. A conserved domain (MAP65_ASE1) was found in all MAP65 proteins. In cucumber, we isolated six CsaMAP65s with different expression patterns in tissues including root, stem, leaf, female flower, male flower, and fruit. Subcellular localizations of CsaMAP65s verified that all CsaMAP65s were localized in microtubule and...
Source: J Appl Genet - May 23, 2023 Category: Genetics & Stem Cells Authors: Meiting Liang Tingting Ji Xueyun Wang Xingyi Wang Shihui Li Lihong Gao Si Ma Yongqiang Tian Source Type: research
