Antimicrobial resistance profile of Aeromonas spp. isolated from asymptomatic Colossoma macropomum cultured in the Amazonas State, Brazil
The objectives of this study were to isolate and identify the main putative pathogenic bacterial species in tambaqui (Colossoma macropomum), establish the profile of resistance to antimicrobials by the methods of disc diffusion, and determine the minimum inhibitory concentration (MIC) values. Two hundred and ninety asymptomatic fish were collected between March and November 2015 from ten fish farms in the Amazonas state (Brazil). Of the total strains recovered from tambaqui, seven were identified as Aeromonas spp. by sequencing the 16S rRNA gene. These seven isolates showed resistance to ampicillin, 28% to erythromycin, an...
Source: Braz J Biol - January 11, 2023 Category: Biology Authors: F A Sebasti ão C Majolo V F S Martins C L Boijink F R Brand ão S L A Pereira R Y Fujimoto E C Chagas Source Type: research

Antimicrobial resistance profile of Aeromonas spp. isolated from asymptomatic Colossoma macropomum cultured in the Amazonas State, Brazil
The objectives of this study were to isolate and identify the main putative pathogenic bacterial species in tambaqui (Colossoma macropomum), establish the profile of resistance to antimicrobials by the methods of disc diffusion, and determine the minimum inhibitory concentration (MIC) values. Two hundred and ninety asymptomatic fish were collected between March and November 2015 from ten fish farms in the Amazonas state (Brazil). Of the total strains recovered from tambaqui, seven were identified as Aeromonas spp. by sequencing the 16S rRNA gene. These seven isolates showed resistance to ampicillin, 28% to erythromycin, an...
Source: Braz J Biol - January 11, 2023 Category: Biology Authors: F A Sebasti ão C Majolo V F S Martins C L Boijink F R Brand ão S L A Pereira R Y Fujimoto E C Chagas Source Type: research

Antimicrobial resistance profile of Aeromonas spp. isolated from asymptomatic Colossoma macropomum cultured in the Amazonas State, Brazil
The objectives of this study were to isolate and identify the main putative pathogenic bacterial species in tambaqui (Colossoma macropomum), establish the profile of resistance to antimicrobials by the methods of disc diffusion, and determine the minimum inhibitory concentration (MIC) values. Two hundred and ninety asymptomatic fish were collected between March and November 2015 from ten fish farms in the Amazonas state (Brazil). Of the total strains recovered from tambaqui, seven were identified as Aeromonas spp. by sequencing the 16S rRNA gene. These seven isolates showed resistance to ampicillin, 28% to erythromycin, an...
Source: Braz J Biol - January 11, 2023 Category: Biology Authors: F A Sebasti ão C Majolo V F S Martins C L Boijink F R Brand ão S L A Pereira R Y Fujimoto E C Chagas Source Type: research

Antimicrobial resistance profile of Aeromonas spp. isolated from asymptomatic Colossoma macropomum cultured in the Amazonas State, Brazil
The objectives of this study were to isolate and identify the main putative pathogenic bacterial species in tambaqui (Colossoma macropomum), establish the profile of resistance to antimicrobials by the methods of disc diffusion, and determine the minimum inhibitory concentration (MIC) values. Two hundred and ninety asymptomatic fish were collected between March and November 2015 from ten fish farms in the Amazonas state (Brazil). Of the total strains recovered from tambaqui, seven were identified as Aeromonas spp. by sequencing the 16S rRNA gene. These seven isolates showed resistance to ampicillin, 28% to erythromycin, an...
Source: Braz J Biol - January 11, 2023 Category: Biology Authors: F A Sebasti ão C Majolo V F S Martins C L Boijink F R Brand ão S L A Pereira R Y Fujimoto E C Chagas Source Type: research

The Meteorin-like cytokine is upregulated in grass carp after infection with Aeromonas hydrophila
In this study, monoclonal antibodies were prepared against recombinant grass carp (Ctenopharyngodon idella, Ci) Metrnl-a in mice and characterized by Western blotting, flow cytometry and immunofluorescent microscopy. In grass carp infected with Aeromonus hydrophila (A. hydrophila), the cells expressing CiMetrnl-a markedly increased in the gills, head kidney and intestine. In the inflamed intestine caused by A. hydrophila infection, the CiMetrnl-a producing cells were detected mainly in the mucosal layer of anterior, middle and posterior segments. Consistently, qRT-PCR analysis showed that the mRNA expression of CiMetrnl-a ...
Source: Developmental and Comparative Immunology - January 7, 2023 Category: Allergy & Immunology Authors: Zhao Jia Jianhua Feng Gaoliang Yuan Hehe Xiao Huifeng Dang Yanwei Zhang Kangyong Chen Jun Zou Junya Wang Source Type: research

The Meteorin-like cytokine is upregulated in grass carp after infection with Aeromonas hydrophila
In this study, monoclonal antibodies were prepared against recombinant grass carp (Ctenopharyngodon idella, Ci) Metrnl-a in mice and characterized by Western blotting, flow cytometry and immunofluorescent microscopy. In grass carp infected with Aeromonus hydrophila (A. hydrophila), the cells expressing CiMetrnl-a markedly increased in the gills, head kidney and intestine. In the inflamed intestine caused by A. hydrophila infection, the CiMetrnl-a producing cells were detected mainly in the mucosal layer of anterior, middle and posterior segments. Consistently, qRT-PCR analysis showed that the mRNA expression of CiMetrnl-a ...
Source: Developmental and Comparative Immunology - January 7, 2023 Category: Allergy & Immunology Authors: Zhao Jia Jianhua Feng Gaoliang Yuan Hehe Xiao Huifeng Dang Yanwei Zhang Kangyong Chen Jun Zou Junya Wang Source Type: research

The Meteorin-like cytokine is upregulated in grass carp after infection with Aeromonas hydrophila
In this study, monoclonal antibodies were prepared against recombinant grass carp (Ctenopharyngodon idella, Ci) Metrnl-a in mice and characterized by Western blotting, flow cytometry and immunofluorescent microscopy. In grass carp infected with Aeromonus hydrophila (A. hydrophila), the cells expressing CiMetrnl-a markedly increased in the gills, head kidney and intestine. In the inflamed intestine caused by A. hydrophila infection, the CiMetrnl-a producing cells were detected mainly in the mucosal layer of anterior, middle and posterior segments. Consistently, qRT-PCR analysis showed that the mRNA expression of CiMetrnl-a ...
Source: Developmental and Comparative Immunology - January 7, 2023 Category: Allergy & Immunology Authors: Zhao Jia Jianhua Feng Gaoliang Yuan Hehe Xiao Huifeng Dang Yanwei Zhang Kangyong Chen Jun Zou Junya Wang Source Type: research

The Meteorin-like cytokine is upregulated in grass carp after infection with Aeromonas hydrophila
In this study, monoclonal antibodies were prepared against recombinant grass carp (Ctenopharyngodon idella, Ci) Metrnl-a in mice and characterized by Western blotting, flow cytometry and immunofluorescent microscopy. In grass carp infected with Aeromonus hydrophila (A. hydrophila), the cells expressing CiMetrnl-a markedly increased in the gills, head kidney and intestine. In the inflamed intestine caused by A. hydrophila infection, the CiMetrnl-a producing cells were detected mainly in the mucosal layer of anterior, middle and posterior segments. Consistently, qRT-PCR analysis showed that the mRNA expression of CiMetrnl-a ...
Source: Developmental and Comparative Immunology - January 7, 2023 Category: Allergy & Immunology Authors: Zhao Jia Jianhua Feng Gaoliang Yuan Hehe Xiao Huifeng Dang Yanwei Zhang Kangyong Chen Jun Zou Junya Wang Source Type: research

CcGSDMEa functions the pore-formation in cytomembrane and the regulation on the secretion of IL-l β in common carp (Cyprinus carpio haematopterus)
GSDME is the only direct executor of caspase-dependent pyroptosis in both canonical and non-canonical inflammasomes known to date in fish, and plays an important role in anti-bacterial infection and inflammatory response. In order to determine the regulation of GSDMEa on antibacterial infection in innate immune response, the CcGSDMEa gene in common carp (Cyprinus carpio haematopterus) was first identified and characterized, and then its function related to immune defense was investigated. Our results showed that the expressions of CcGSDMEa at the mRNA and protein levels were both significantly increased after Aeromonas hyd...
Source: Frontiers in Immunology - January 6, 2023 Category: Allergy & Immunology Source Type: research

New Insights for tracking bacterial community structures in industrial wastewater from textile factories to surface water using phenotypic, 16S rRNA isolates identifications and High-throughput sequencing
In conclusion, the microbial communities in textile wastewaters are similar to those in environmental samples at the phylum level but distinct at the genus and species levels because they are exposed to a wider range of environmental circumstances.PMID:36574894 | DOI:10.1016/j.actatropica.2022.106806 (Source: Acta Tropica)
Source: Acta Tropica - December 27, 2022 Category: Infectious Diseases Authors: Mohamed Azab El-Liethy Bahaa A Hemdan Gamila E El-Taweel Source Type: research

Investigation of antibiotic-resistant vibrios associated with shrimp (Penaeus vannamei) farms
Arch Microbiol. 2022 Dec 26;205(1):41. doi: 10.1007/s00203-022-03376-w.ABSTRACTFor the sustainable farming of disease-free and healthy shrimps, antimicrobial use is frequent nowadays in shrimp-cultured system. Considering the serious impact of global antimicrobial resistance (AMR), the present study was focused to investigate the prevalence of antimicrobial-resistant vibrios among infected shrimps (Penaeus vannamei) from two brackish water-cultured farms. Diverse species of vibrios viz. V. alginolyticus, V. parahaemolyticus, V. cholerae, V. mimicus, and V. fluvialis along with Aeromonas hydrophila, A. salmonicida and Shewa...
Source: Archives of Microbiology - December 26, 2022 Category: Microbiology Authors: Das Prabina T Raja Swaminathan Sowmya P Mohandas J C Anjana K Manjusha P G Preena Source Type: research

Investigation of antibiotic-resistant vibrios associated with shrimp (Penaeus vannamei) farms
Arch Microbiol. 2022 Dec 26;205(1):41. doi: 10.1007/s00203-022-03376-w.ABSTRACTFor the sustainable farming of disease-free and healthy shrimps, antimicrobial use is frequent nowadays in shrimp-cultured system. Considering the serious impact of global antimicrobial resistance (AMR), the present study was focused to investigate the prevalence of antimicrobial-resistant vibrios among infected shrimps (Penaeus vannamei) from two brackish water-cultured farms. Diverse species of vibrios viz. V. alginolyticus, V. parahaemolyticus, V. cholerae, V. mimicus, and V. fluvialis along with Aeromonas hydrophila, A. salmonicida and Shewa...
Source: Archives of Microbiology - December 26, 2022 Category: Microbiology Authors: Das Prabina T Raja Swaminathan Sowmya P Mohandas J C Anjana K Manjusha P G Preena Source Type: research

Biofilm formation of pathogenic bacteria isolated from aquatic animals
Arch Microbiol. 2022 Dec 24;205(1):36. doi: 10.1007/s00203-022-03332-8.ABSTRACTBacterial biofilm formation is one of the dynamic processes, which facilitates bacteria cells to attach to a surface and accumulate as a colony. With the help of biofilm formation, pathogenic bacteria can survive by adapting to their external environment. These bacterial colonies have several resistance properties with a higher survival rate in the environment. Especially, pathogenic bacteria can grow as biofilms and can be protected from antimicrobial compounds and other substances. In aquaculture, biofilm formation by pathogenic bacteria has e...
Source: Archives of Microbiology - December 24, 2022 Category: Microbiology Authors: L A D S De Silva Gang-Joon Heo Source Type: research

Expression and functional characterization of three β-defensins in grass carp (Ctenopharyngodon idella)
In this study, the expression and antibacterial activities of three grass carp (Ctenopharyngodon idella) (Ci) β-defensin (BD) peptides were comparatively investigated. Expression analysis reveals that CiBD1-3 were constitutively expressed in tissues, with the highest expression detected in the skin. The CiBD-1 transcripts were more abundant than CiBD-2 and CiBD-3. In the primary head kidney leukocytes, CiBDs were induced by PHA, LPS, poly(I:C) and cytokines such as IL-1β and IFN-γ. In vivo challenge of fish with Aeromonas hydrophila resulted in the up-regulation of CiBDs in the head kidney and hindgut. To determine the ...
Source: Developmental and Comparative Immunology - December 24, 2022 Category: Allergy & Immunology Authors: Jianhua Feng Zhao Jia Gaoliang Yuan Xiaozhen Zhu Qin Liu Kaizheng Wu Junya Wang Jun Zou Source Type: research

Biofilm formation of pathogenic bacteria isolated from aquatic animals
Arch Microbiol. 2022 Dec 24;205(1):36. doi: 10.1007/s00203-022-03332-8.ABSTRACTBacterial biofilm formation is one of the dynamic processes, which facilitates bacteria cells to attach to a surface and accumulate as a colony. With the help of biofilm formation, pathogenic bacteria can survive by adapting to their external environment. These bacterial colonies have several resistance properties with a higher survival rate in the environment. Especially, pathogenic bacteria can grow as biofilms and can be protected from antimicrobial compounds and other substances. In aquaculture, biofilm formation by pathogenic bacteria has e...
Source: Archives of Microbiology - December 24, 2022 Category: Microbiology Authors: L A D S De Silva Gang-Joon Heo Source Type: research