Comparative resistome analysis of Aeromonas species in aquaculture reveals antibiotic resistance patterns and phylogeographic distribution
Environ Res. 2023 Oct 5:117273. doi: 10.1016/j.envres.2023.117273. Online ahead of print.ABSTRACTThe overuse of antibiotics in aquaculture drives the emergence of multi-drug-resistant bacteria, and antibiotic-resistant genes (ARGs) can be disseminated to other bacteria through vertical- and horizontal gene transfer (VGT and HGT) under selective pressure. Profiling the antibiotic resistome and understanding the global distribution of ARGs constitutes the first step in developing a control strategy. Hence, this study utilized extensive genomic data from hundreds of Aeromonas strains in aquaculture to profile resistome patter...
Source: Environmental Research - October 7, 2023 Category: Environmental Health Authors: HyeongJin Roh Dhamotharan Kannimuthu Source Type: research
The antivirulence activity, transcriptomics of EGCG and its protective effects on zebrafish infected by Aeromonas hydrophila
ConclusionEGCG would be a potential alternative drug for the prevention and treatment of A. hydrophila infections by anti-virulence mechanism. (Source: Frontiers in cellular and infection microbiology)
Source: Frontiers in cellular and infection microbiology - October 6, 2023 Category: Microbiology Source Type: research
DNA vaccine incorporated poly (lactic-co-glycolic) acid (PLGA) microspheres offer enhanced protection against Aeromonas hydrophila infection
Int J Biol Macromol. 2023 Oct 2:127182. doi: 10.1016/j.ijbiomac.2023.127182. Online ahead of print.ABSTRACTEncapsulation of DNA vaccines onto carriers enhances the immunogenicity of an antigen. Specifically, biodegradable polymers offer sustained release of vaccines which is crucial for any targeted delivery approach. Poly (lactic-co-glycolic) acid (PLGA) microspheres were used to load a DNA vaccine having a targeted gene of outer membrane protein (OMP) of Aeromonas hydrophila to clone and construct a DNA vaccine using a eukaryotic expression vector system (pVAX1-OMP DNA) of A. hydrophila infection in fish. PLGA microspher...
Source: International Journal of Biological Macromolecules - October 4, 2023 Category: Biochemistry Authors: Eswaramoorthy Thirumalaikumar S Vimal Ramamoorthy Sathishkumar M Ravi V Karthick S Ramya John Thomas Vinay Kumar Chinnaperumal Kamaraj Thavasimuthu Citarasu Source Type: research
DNA vaccine incorporated poly (lactic-co-glycolic) acid (PLGA) microspheres offer enhanced protection against Aeromonas hydrophila infection
Int J Biol Macromol. 2023 Oct 2:127182. doi: 10.1016/j.ijbiomac.2023.127182. Online ahead of print.ABSTRACTEncapsulation of DNA vaccines onto carriers enhances the immunogenicity of an antigen. Specifically, biodegradable polymers offer sustained release of vaccines which is crucial for any targeted delivery approach. Poly (lactic-co-glycolic) acid (PLGA) microspheres were used to load a DNA vaccine having a targeted gene of outer membrane protein (OMP) of Aeromonas hydrophila to clone and construct a DNA vaccine using a eukaryotic expression vector system (pVAX1-OMP DNA) of A. hydrophila infection in fish. PLGA microspher...
Source: International Journal of Biological Macromolecules - October 4, 2023 Category: Biochemistry Authors: Eswaramoorthy Thirumalaikumar S Vimal Ramamoorthy Sathishkumar M Ravi V Karthick S Ramya John Thomas Vinay Kumar Chinnaperumal Kamaraj Thavasimuthu Citarasu Source Type: research
Effect of glyphosate-based herbicide roundup on hemato-biochemistry of Labeo rohita (Hamilton, 1822) and susceptibility to Aeromonas hydrophila infection
Environ Sci Pollut Res Int. 2023 Oct 2. doi: 10.1007/s11356-023-29967-8. Online ahead of print.ABSTRACTIn the present study, comprehensive research was executed to investigate the salient toxic effects of glyphosate herbicide in static water system by evaluating the haemato-biochemical profiles of Labio rohita. A challenge study against Aeromonas hydrophila was conducted to determine disease susceptibility of the fish, treated to varying concentrations of commercial-grade glyphosate herbicide. A static range finding bioassay and definitive test revealed that the 96-h LC50 value of glyphosate was 10.16 mg L-1. The experimen...
Source: Environmental Science and Pollution Research International - October 2, 2023 Category: Environmental Health Authors: Khaidem Rabina Chanu Yumnam Abungcha Mangang Sourabh Debbarma Pramod Kumar Pandey Source Type: research
(R/S)-lactate/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by Ralstonia eutropha
In this study, the sequence-regulating PhaCAR was applied in the natural PHA-producing bacterium, R. eutropha. During the investigation, (R/S)-2HB was found to exhibit strong growth inhibitory effects on the cells of R. eutropha. This was probably due to formation of excess 2-ketobutyrate (2KB) from (R/S)-2HB and the consequent L-valine depletion caused by dominant L-isoleucine synthesis attributed to the excess 2KB. Deletion analyses for genes of lactate dehydrogenase homologs identified cytochrome-dependent D-lactate dehydrogenase (Dld) and [Fe-S] protein-dependent L-lactate dehydrogenase as the enzymes responsible for s...
Source: Applied Microbiology and Biotechnology - September 29, 2023 Category: Microbiology Authors: Shizuru Ishihara Izumi Orita Ken'ichiro Matsumoto Toshiaki Fukui Source Type: research
Whole genome sequence analysis of Aeromonas-infecting bacteriophage AHPMCC7, a new species of genus Ahphunavirus and its application in Litopenaeus vannamei culture
In this study, we evaluated the efficacy of bacteriophage AHPMCC7 which was isolated by using A. hydrophila MTCC 1739 as a host. This bacteriophage exhibited 10 min latent period and burst size was 275. In liquid culture, bacteriophage AHPMCC7 could completely lyse A. hydrophila MTCC 1739 after 2 h. AHPMCC7 genome was 42,277 bp long with 58.9% G + C content. The genome consisted of 48 CDSs and no tRNA. The comparative genomic analyses clearly implied that AHPMCC7 might represent a novel species of the genus Aphunavirus under Autographiviridae family. Bacteriophage AHPMCC7 could survive at broad pH (3-10), temperature (4-37...
Source: Virology - September 29, 2023 Category: Virology Authors: Smita Ghosh Priyanka Kar Sudipta Chakrabarti Shrabani Pradhan Keshab Chandra Mondal Kuntal Ghosh Source Type: research
(R/S)-lactate/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by Ralstonia eutropha
In this study, the sequence-regulating PhaCAR was applied in the natural PHA-producing bacterium, R. eutropha. During the investigation, (R/S)-2HB was found to exhibit strong growth inhibitory effects on the cells of R. eutropha. This was probably due to formation of excess 2-ketobutyrate (2KB) from (R/S)-2HB and the consequent L-valine depletion caused by dominant L-isoleucine synthesis attributed to the excess 2KB. Deletion analyses for genes of lactate dehydrogenase homologs identified cytochrome-dependent D-lactate dehydrogenase (Dld) and [Fe-S] protein-dependent L-lactate dehydrogenase as the enzymes responsible for s...
Source: Applied Microbiology and Biotechnology - September 29, 2023 Category: Microbiology Authors: Shizuru Ishihara Izumi Orita Ken'ichiro Matsumoto Toshiaki Fukui Source Type: research
Whole genome sequence analysis of Aeromonas-infecting bacteriophage AHPMCC7, a new species of genus Ahphunavirus and its application in Litopenaeus vannamei culture
In this study, we evaluated the efficacy of bacteriophage AHPMCC7 which was isolated by using A. hydrophila MTCC 1739 as a host. This bacteriophage exhibited 10 min latent period and burst size was 275. In liquid culture, bacteriophage AHPMCC7 could completely lyse A. hydrophila MTCC 1739 after 2 h. AHPMCC7 genome was 42,277 bp long with 58.9% G + C content. The genome consisted of 48 CDSs and no tRNA. The comparative genomic analyses clearly implied that AHPMCC7 might represent a novel species of the genus Aphunavirus under Autographiviridae family. Bacteriophage AHPMCC7 could survive at broad pH (3-10), temperature (4-37...
Source: Virology - September 29, 2023 Category: Virology Authors: Smita Ghosh Priyanka Kar Sudipta Chakrabarti Shrabani Pradhan Keshab Chandra Mondal Kuntal Ghosh Source Type: research
(R/S)-lactate/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by Ralstonia eutropha
In this study, the sequence-regulating PhaCAR was applied in the natural PHA-producing bacterium, R. eutropha. During the investigation, (R/S)-2HB was found to exhibit strong growth inhibitory effects on the cells of R. eutropha. This was probably due to formation of excess 2-ketobutyrate (2KB) from (R/S)-2HB and the consequent L-valine depletion caused by dominant L-isoleucine synthesis attributed to the excess 2KB. Deletion analyses for genes of lactate dehydrogenase homologs identified cytochrome-dependent D-lactate dehydrogenase (Dld) and [Fe-S] protein-dependent L-lactate dehydrogenase as the enzymes responsible for s...
Source: Applied Microbiology and Biotechnology - September 29, 2023 Category: Microbiology Authors: Shizuru Ishihara Izumi Orita Ken'ichiro Matsumoto Toshiaki Fukui Source Type: research
(R/S)-lactate/2-hydroxybutyrate dehydrogenases in and biosynthesis of block copolyesters by Ralstonia eutropha
In this study, the sequence-regulating PhaCAR was applied in the natural PHA-producing bacterium, R. eutropha. During the investigation, (R/S)-2HB was found to exhibit strong growth inhibitory effects on the cells of R. eutropha. This was probably due to formation of excess 2-ketobutyrate (2KB) from (R/S)-2HB and the consequent L-valine depletion caused by dominant L-isoleucine synthesis attributed to the excess 2KB. Deletion analyses for genes of lactate dehydrogenase homologs identified cytochrome-dependent D-lactate dehydrogenase (Dld) and [Fe-S] protein-dependent L-lactate dehydrogenase as the enzymes responsible for s...
Source: Applied Microbiology and Biotechnology - September 29, 2023 Category: Microbiology Authors: Shizuru Ishihara Izumi Orita Ken'ichiro Matsumoto Toshiaki Fukui Source Type: research
Isolation and characterization of a lectin-like chitinase from the hepatopancreas of freshwater prawn, Macrobrachium rosenbergii
This study suggests that the chitinase 1 protein with lectin-like properties is possibly induced at the protein level and can be putatively involved in the innate immune response of M. rosenbergii.PMID:37769935 | DOI:10.1016/j.biochi.2023.09.025 (Source: Biochimie)
Source: Biochimie - September 28, 2023 Category: Biochemistry Authors: Sonalina Sahoo Mohan R Badhe Anirban Paul Pramoda Kumar Sahoo Amol R Suryawanshi Debabrata Panda Bindu R Pillai Snigdha Baliarsingh Bharat Bhusan Patnaik Jyotirmaya Mohanty Source Type: research
Isolation and characterization of a lectin-like chitinase from the hepatopancreas of freshwater prawn, Macrobrachium rosenbergii
This study suggests that the chitinase 1 protein with lectin-like properties is possibly induced at the protein level and can be putatively involved in the innate immune response of M. rosenbergii.PMID:37769935 | DOI:10.1016/j.biochi.2023.09.025 (Source: Biochimie)
Source: Biochimie - September 28, 2023 Category: Biochemistry Authors: Sonalina Sahoo Mohan R Badhe Anirban Paul Pramoda Kumar Sahoo Amol R Suryawanshi Debabrata Panda Bindu R Pillai Snigdha Baliarsingh Bharat Bhusan Patnaik Jyotirmaya Mohanty Source Type: research
Synthesis of Silver Nanoparticles from Aeromonas caviae for Antibacterial Activity and In Vivo Effects in Rats
Biol Trace Elem Res. 2023 Sep 27. doi: 10.1007/s12011-023-03876-w. Online ahead of print.ABSTRACTSilver nanoparticles (AgNPs) have excellent antimicrobial properties, as they can inhibit multidrug-resistant (MDR) pathogens. Furthermore, bio-AgNPs have potential applications in medicine due to their low toxicity and high stability. Here, AgNPs were synthesized from the biomass of Aeromonas caviae isolated from a sediment sample and subsequently characterized. The UV-Vis spectra of AgNPs in aqueous medium peaked at 417 nm, matching their plasmon absorption. The X-ray diffraction analysis (XRD) pattern of AgNPs showed four pe...
Source: Biological Trace Element Research - September 26, 2023 Category: Biology Authors: Safin Hussein Saman Sulaiman Seenaa Ali Rzgar Pirot Karzan Qurbani Haider Hamzah Omed Hassan Treefa Ismail Sirwan Khalid Ahmed Zahra Azizi Source Type: research
16S rRNA and transcriptome analysis of the FOS-mediated alleviation of Aeromonas hydrophila-induced intestinal damage in Megalobrama amblycephala
This study was conducted to elucidate the effects of FOS that alleviate Aeromonas hydrophila-induced intestinal damage. The results showed that A. hydrophila disrupted the intestinal structure and increased intestinal permeability, causing abnormalities in mucosal pathology. Additionally, A. hydrophila induced an imbalance in the intestinal flora and disturbed its stability. Dietary FOS ameliorated the injury to the intestinal structure of fish, but also in part improved the condition of the intestinal tight junction complex. Transcriptomic analysis showed that 120 genes were up-regulated and 320 genes were down-regulated....
Source: International Journal of Biological Macromolecules - September 24, 2023 Category: Biochemistry Authors: Dongxue Jiang Shengnan Li Yuexia Liang Ruiyi Xu Qian Qi Bingke Wang Chunnuan Zhang Source Type: research
