Effect of long-term temperature stress on the intestinal microbiome of an invasive snail
In this study, the morphological changes and intestinal microbiome of P. canaliculata under long-term stress at low temperature (15°C) and high temperature (35°C) were investigated with laboratory experiments. Compared with control group (25°C), the alpha diversity increased and pathogenic bacteria enriched changed under high and low temperature stress. The effect of high temperature stress on the intestinal microbiome of P. canaliculata was more significant than that of low temperature stress. A sustained high temperature environment led to an increase in the abundance of pathogenic bacteria, such as Aeromonas and Ente...
Source: Frontiers in Microbiology - August 29, 2022 Category: Microbiology Source Type: research

Genome dynamics, codon usage patterns and influencing factors in Aeromonas hydrophila phages
Virus Res. 2022 Aug 24:198900. doi: 10.1016/j.virusres.2022.198900. Online ahead of print.ABSTRACTIn the present study, genome characteristics and codon usage patterns of 44 Aeromonas hydrophila phages were studied. Phage genomes varied from 30.8 to 262.0 kb with mean±SD and median values of 111.3±81.4 kb and 79.4 kb, respectively. Though the great variation in phage GC contents (35.1% - 62.2%) was observed, GC contents of all phages (except two phages) were significantly less than the GC content (62.4±5.6%) of the host. The effective number of codons (ENC) values of phage genes ranged from 27.7 to 61 with a mean±SD va...
Source: Virus Research - August 27, 2022 Category: Virology Authors: Anuj Tyagi Vandan Nagar Source Type: research

Genome dynamics, codon usage patterns and influencing factors in Aeromonas hydrophila phages
Virus Res. 2022 Aug 24:198900. doi: 10.1016/j.virusres.2022.198900. Online ahead of print.ABSTRACTIn the present study, genome characteristics and codon usage patterns of 44 Aeromonas hydrophila phages were studied. Phage genomes varied from 30.8 to 262.0 kb with mean±SD and median values of 111.3±81.4 kb and 79.4 kb, respectively. Though the great variation in phage GC contents (35.1% - 62.2%) was observed, GC contents of all phages (except two phages) were significantly less than the GC content (62.4±5.6%) of the host. The effective number of codons (ENC) values of phage genes ranged from 27.7 to 61 with a mean±SD va...
Source: Virus Research - August 27, 2022 Category: Virology Authors: Anuj Tyagi Vandan Nagar Source Type: research

Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida
The liver is a multitasking organ with essential functions for vertebrate health spanning metabolism and immunity. In contrast to mammals, our understanding of liver cellular heterogeneity and its role in regulating immunological status remains poorly defined in fishes. Addressing this knowledge gap, we generated a transcriptomic atlas of 47,432 nuclei isolated from the liver of Atlantic salmon (Salmo salar L.) contrasting control fish with those challenged with a pathogenic strain of Aeromonas salmonicida, a problematic bacterial pathogen in global aquaculture. We identified the major liver cell types and their sub-popula...
Source: Frontiers in Immunology - August 24, 2022 Category: Allergy & Immunology Source Type: research

Metagenomic Insights into the Gut Microbiota of Eudrilus eugeniae (Kinberg) and Its Potential Roles in Agroecosystem
Curr Microbiol. 2022 Aug 22;79(10):295. doi: 10.1007/s00284-022-02988-1.ABSTRACTGut microbiomes, a consortium of microorganisms that live in the animal gut, are highly engineered microbial communities. It makes a major contribution to digestive health, metabolism management, and the development of a strong immune system in the host. The present study was taken up to answer the long-running question about the existence of truly indigenous microflora of the epigeic earthworm gut. This is due to the general difficulties of culturing many of the microorganisms found in soil or earthworms' gut. Keeping this fact in a view, the ...
Source: Current Microbiology - August 21, 2022 Category: Microbiology Authors: Samrendra Singh Thakur Azhar Rashid Lone Sailu Yellaboina Subodh Tambat Ajar Nath Yadav Subodh Kumar Jain Shweta Yadav Source Type: research

Metagenomic Insights into the Gut Microbiota of Eudrilus eugeniae (Kinberg) and Its Potential Roles in Agroecosystem
Curr Microbiol. 2022 Aug 22;79(10):295. doi: 10.1007/s00284-022-02988-1.ABSTRACTGut microbiomes, a consortium of microorganisms that live in the animal gut, are highly engineered microbial communities. It makes a major contribution to digestive health, metabolism management, and the development of a strong immune system in the host. The present study was taken up to answer the long-running question about the existence of truly indigenous microflora of the epigeic earthworm gut. This is due to the general difficulties of culturing many of the microorganisms found in soil or earthworms' gut. Keeping this fact in a view, the ...
Source: Current Microbiology - August 21, 2022 Category: Microbiology Authors: Samrendra Singh Thakur Azhar Rashid Lone Sailu Yellaboina Subodh Tambat Ajar Nath Yadav Subodh Kumar Jain Shweta Yadav Source Type: research

Disaggregation as an interaction mechanism among intestinal bacteria
In this study, we focus on bacterial species of genera Aeromonas and Enterobacter. Two zebrafish gut derived isolates, Aeromonas ZOR0001 (AE) and Enterobacter ZOR0014 (EN), when mono-associated with the host, are highly aggregated and located primarily in the intestinal midgut. An Aeromonas isolate derived from the commensal strain, Aeromonas-MB4 (AE-MB4), differs from the parental strain in that it is composed mostly of planktonic cells localized to the anterior gut. When challenged by AE-MB4, clusters of EN rapidly fragment into non-motile, slow-growing, dispersed individual cells with overall abundance two orders of mag...
Source: Biophysical Journal - August 19, 2022 Category: Physics Authors: Deepika Sundarraman T Jarrod Smith Jade V Z Kast Karen Guillemin Raghuveer Parthasarathy Source Type: research

Disaggregation as an interaction mechanism among intestinal bacteria
In this study, we focus on bacterial species of genera Aeromonas and Enterobacter. Two zebrafish gut derived isolates, Aeromonas ZOR0001 (AE) and Enterobacter ZOR0014 (EN), when mono-associated with the host, are highly aggregated and located primarily in the intestinal midgut. An Aeromonas isolate derived from the commensal strain, Aeromonas-MB4 (AE-MB4), differs from the parental strain in that it is composed mostly of planktonic cells localized to the anterior gut. When challenged by AE-MB4, clusters of EN rapidly fragment into non-motile, slow-growing, dispersed individual cells with overall abundance two orders of mag...
Source: Biophysical Journal - August 19, 2022 Category: Physics Authors: Deepika Sundarraman T Jarrod Smith Jade V Z Kast Karen Guillemin Raghuveer Parthasarathy Source Type: research

GSE207655 Single cell transcriptomics of Atlantic salmon (Salmo salar L.) liver reveals cellular heterogeneity and immunological responses to challenge by Aeromonas salmonicida
Contributors : R S Taylor ; R R Daniels ; R Dobie ; S Naseer ; Thomas C Clark ; Neil C Henderson ; P Boudinot ; Samuel A Martin ; D J MacqueenSeries Type : Expression profiling by high throughput sequencingOrganism : Salmo salarThe liver is a multitasking organ with essential functions for vertebrate health spanning metabolism and immunity. To explore the multifaceted role played by liver cells in response to bacterial infection in fishes, we generated a transcriptomic atlas of nuclei isolated from the liver of Atlantic salmon (Salmo salar L.), contrasting control fish with those challenged with a pathogenic strain of A...
Source: GEO: Gene Expression Omnibus - August 16, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Salmo salar Source Type: research

Epidemiological Cutoff Values for Standard Broth Microdilution and Disk Diffusion Susceptibility Testing of Aeromonas hydrophila Isolated from Fish
Microbial Drug Resistance,Volume 28, Issue 8, Page 893-903, August 2022. (Source: Microbial Drug Resistance)
Source: Microbial Drug Resistance - August 16, 2022 Category: Microbiology Authors: Charles M. Gieseker Patricia S. Gaunt John P. Hawke Tina C. Crosby Nicholas R. Hasbrouck Dana X. Gao Cynthia B. Stine Eric R. Evans Christopher J. Grim Source Type: research

Incidence, evaluation of detection and identification methods, and antimicrobial resistance of Aeromonas spp. in ready-to-eat foods
This study aimed to investigate the prevalence of Aeromonas spp. in ready-to-eat foods (temakis, cheeses and minimally processed fruits) and to characterize the virulence profile and antimicrobial resistance of the isolates. The species A. hydrophila, A. caviae and A. veronii were identified using polymerase chain reaction (PCR), which was later compared with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The performance of two isolation selective agars (starch-ampicillin agar-SAA and Aeromonas agar-AA) was also evaluated. Aeromonas spp. was isolated in 66.67 % (20/30) of temak...
Source: International Journal of Food Microbiology - August 13, 2022 Category: Food Science Authors: Josie Roberta Teodoro Gabriela Guimar ães Carvalho Murilo Mariz Queiroz Carlos Emilio Levy Dirce Yorika Kabuki Source Type: research

Incidence, evaluation of detection and identification methods, and antimicrobial resistance of Aeromonas spp. in ready-to-eat foods
This study aimed to investigate the prevalence of Aeromonas spp. in ready-to-eat foods (temakis, cheeses and minimally processed fruits) and to characterize the virulence profile and antimicrobial resistance of the isolates. The species A. hydrophila, A. caviae and A. veronii were identified using polymerase chain reaction (PCR), which was later compared with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The performance of two isolation selective agars (starch-ampicillin agar-SAA and Aeromonas agar-AA) was also evaluated. Aeromonas spp. was isolated in 66.67 % (20/30) of temak...
Source: International Journal of Food Microbiology - August 13, 2022 Category: Food Science Authors: Josie Roberta Teodoro Gabriela Guimar ães Carvalho Murilo Mariz Queiroz Carlos Emilio Levy Dirce Yorika Kabuki Source Type: research

Incidence, evaluation of detection and identification methods, and antimicrobial resistance of Aeromonas spp. in ready-to-eat foods
This study aimed to investigate the prevalence of Aeromonas spp. in ready-to-eat foods (temakis, cheeses and minimally processed fruits) and to characterize the virulence profile and antimicrobial resistance of the isolates. The species A. hydrophila, A. caviae and A. veronii were identified using polymerase chain reaction (PCR), which was later compared with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The performance of two isolation selective agars (starch-ampicillin agar-SAA and Aeromonas agar-AA) was also evaluated. Aeromonas spp. was isolated in 66.67 % (20/30) of temak...
Source: International Journal of Food Microbiology - August 13, 2022 Category: Food Science Authors: Josie Roberta Teodoro Gabriela Guimar ães Carvalho Murilo Mariz Queiroz Carlos Emilio Levy Dirce Yorika Kabuki Source Type: research

Antibiotic-induced alternations in gut microflora are associated with the suppression of immune-related pathways in grass carp (Ctenopharyngodon idellus)
Gut microbiota play a vital role in fish health homeostasis. Antibiotics are known to alter microbial community composition and diversity; however, the substantial effects of antibiotics upon the gut microbiome with respect to immune-related pathways in healthy fish remain unclear. Accordingly, here we explored the impact of two antibiotics on the intestinal health, immune response, microbiome dynamics, and transcriptome profiles of grass carp. A two-week feeding trial was carried out in which the basal diet was complemented with enrofloxacin (10 mg/kg) or florfenicol (10 mg/kg). The results showed that: (1) Enrofloxacin a...
Source: Frontiers in Immunology - August 11, 2022 Category: Allergy & Immunology Source Type: research

Characterization and genomic analysis of the novel Aeromonas veronii phage pAEv1812
AbstractBacteria of the speciesAeromonas veronii are generally ubiquitous in different aquatic environments and are capable of causing a variety of diseases in aquatic animals. In this work, the phage isolate pAEv1812, which is lytic toA. veronii strain 1810, was characterized and sequenced. Morphological analysis revealed that pAEv1812 has a symmetrical head and a long non-contractile tail. A one-step growth curve analysis showed that the latent period and burst period of pAEv1812 were approximately 40 min and 80 min, respectively, with a burst size of 190 PFU/infected cell. The genome of phage pAEv1812 is 61,461 bp in le...
Source: Archives of Virology - August 8, 2022 Category: Virology Source Type: research