Heterogeneous glycosylation and methylation of the Aeromonas caviae flagellin
Flagellin glycosylation is required for swimming motility inAeromonas. Here, we show that O-glycosylation was heterogeneous with no serine or threonine sites that were constantly glycosylated, and mutation of several sites was found to affect motility. Data herein suggest that hydrophobic amino acids play a role in the glycosylation process. AbstractBacterial swimming is mediated by the rotation of a flagellar filament. Many bacteria are now known to be able toO-glycosylate their flagellins, the proteins that make up the flagellar filament. For bacteria that use nonulosonic acid sugars such as pseudaminic acid, this glycos...
Source: MicrobiologyOpen - July 19, 2022 Category: Microbiology Authors: Rebecca C. Lowry, Laila Allihaybi, Jennifer L. Parker, Narciso A. S. Couto, Graham P. Stafford, Jonathan G. Shaw Tags: ORIGINAL ARTICLE Source Type: research

Effects of four endophytic bacteria on cadmium speciation and remediation efficiency of Sedum plumbizincicola in farmland soil
This study was aimed to improve the remediation effect of S. plumbizincicola on Cd-contaminated farmland soil and provide a theoretical basis for the enhancement of endophytic bacteria in the repair of Cd-contaminated soil with S. plumbizincicola. Four kinds of endophytic bacteria, namely Buttiauxella, Pedobacter, Aeromonas eucrenophila, and Ralstonia pickettii, were used, and soil culture experiments and pot experiments were conducted to explore the effects of endophytic bacteria on soil Cd speciation and phytoremediation efficiency of Cd-contaminated farmland soils. Under the experimental conditions, after inoculation wi...
Source: Environmental Science and Pollution Research International - July 19, 2022 Category: Environmental Health Authors: Xueyu Cheng Xueying Cao Changyin Tan Lulu Liu Jia Bai Yufeng Liang Runzhong Cai Source Type: research

Effects of four endophytic bacteria on cadmium speciation and remediation efficiency of Sedum plumbizincicola in farmland soil
This study was aimed to improve the remediation effect of S. plumbizincicola on Cd-contaminated farmland soil and provide a theoretical basis for the enhancement of endophytic bacteria in the repair of Cd-contaminated soil with S. plumbizincicola. Four kinds of endophytic bacteria, namely Buttiauxella, Pedobacter, Aeromonas eucrenophila, and Ralstonia pickettii, were used, and soil culture experiments and pot experiments were conducted to explore the effects of endophytic bacteria on soil Cd speciation and phytoremediation efficiency of Cd-contaminated farmland soils. Under the experimental conditions, after inoculation wi...
Source: Environmental Science and Pollution Research International - July 19, 2022 Category: Environmental Health Authors: Xueyu Cheng Xueying Cao Changyin Tan Lulu Liu Jia Bai Yufeng Liang Runzhong Cai Source Type: research

Sterile alpha and TIR motif-containing protein 1 is a negative regulator in the anti-bacterial immune responses in nile tilapia (Oreochromis niloticus)
In this study, O. niloticus SARM1 (OnSARM1) was cloned and its evolutionary status was verified using bioinformatic analyses. mRNA expression of OnSARM1 was found at a higher level in the trunk kidney and muscle in healthy fish. The examination of its subcellular location showed that the OnSARM1 was detected only in the cytoplasm of THK cells, and colocalized with OnMyD88, OnTRIF and OnTRIF in small speckle-like condensed granules. The transcript levels of OnMyD88, OnTIRAP, OnTRIF, and downstream effectors, including interleukin (IL)-1β, IL-8, IL-12b and type I interferon (IFN)d2.13, were regulated conversely to the expre...
Source: Frontiers in Immunology - July 19, 2022 Category: Allergy & Immunology Source Type: research

Host Species Influence the Gut Microbiota of Endemic Cold-Water Fish in Upper Yangtze River
In this study, the gut samples of Schizothorax wangchiachii (SW, herbivorous), Schizothorax kozlovi (SK, omnivorous), and Percocypris pingi (PP, carnivorous) in the upper Yangtze River were collected, and we sequenced 16S rRNA amplicon to study the potential relationship between gut microbes and host species. The results showed that gut microbial composition and diversity were significantly different between the three cold-water fishes. These fishes had different key taxa in their gut microbes, including bacteria involved in the breakdown of food (e.g., Cetobacterium, Aeromonas, and Clostridium sensu stricto 10). The highe...
Source: Frontiers in Microbiology - July 18, 2022 Category: Microbiology Source Type: research

Profiling microalgal cultures growing on municipal wastewater and fertilizer media in raceway photobioreactors
Bioresour Technol. 2022 Jul 13:127619. doi: 10.1016/j.biortech.2022.127619. Online ahead of print.ABSTRACTMicroalgae cultivation is proposed as an effective system for pathogens reduction and wastewater depuration, however, a full characterisation of the risks is still needed. Two raceways were inoculated with Scenedesmus, one using wastewater and the other using a fertilizer medium. Microbial community and pathogen presence were explored by next generation sequencing (NGS), commercial qPCR array and plate counts. These methods proved to be complementary for a full characterization of community structure and potential risk...
Source: Bioresource Technology - July 16, 2022 Category: Biotechnology Authors: Elisa Clagnan Giuliana D'Imporzano Marta Dell'Orto Ana Sanchez-Zurano Francisco Gabriel Aci én-Fernandez Biancamaria Pietrangeli Fabrizio Adani Source Type: research

Profiling microalgal cultures growing on municipal wastewater and fertilizer media in raceway photobioreactors
Bioresour Technol. 2022 Jul 13;360:127619. doi: 10.1016/j.biortech.2022.127619. Online ahead of print.ABSTRACTMicroalgae cultivation is proposed as an effective system for pathogens reduction and wastewater depuration, however, a full characterisation of the risks is still needed. Two raceways were inoculated with Scenedesmus, one using wastewater and the other using a fertilizer medium. Microbial community and pathogen presence were explored by next generation sequencing (NGS), commercial qPCR array and plate counts. These methods proved to be complementary for a full characterization of community structure and potential ...
Source: Bioresource Technology - July 16, 2022 Category: Biotechnology Authors: Elisa Clagnan Giuliana D'Imporzano Marta Dell'Orto Ana Sanchez-Zurano Francisco Gabriel Aci én-Fernandez Biancamaria Pietrangeli Fabrizio Adani Source Type: research

Profiling microalgal cultures growing on municipal wastewater and fertilizer media in raceway photobioreactors
Bioresour Technol. 2022 Jul 13;360:127619. doi: 10.1016/j.biortech.2022.127619. Online ahead of print.ABSTRACTMicroalgae cultivation is proposed as an effective system for pathogens reduction and wastewater depuration, however, a full characterisation of the risks is still needed. Two raceways were inoculated with Scenedesmus, one using wastewater and the other using a fertilizer medium. Microbial community and pathogen presence were explored by next generation sequencing (NGS), commercial qPCR array and plate counts. These methods proved to be complementary for a full characterization of community structure and potential ...
Source: Bioresource Technology - July 16, 2022 Category: Biotechnology Authors: Elisa Clagnan Giuliana D'Imporzano Marta Dell'Orto Ana Sanchez-Zurano Francisco Gabriel Aci én-Fernandez Biancamaria Pietrangeli Fabrizio Adani Source Type: research

Dietary chitosan modulates the growth performance, body composition and nonspecific immunity of juvenile yellow catfish (Pelteobagrus fulvidraco)
This study investigated the effects of feeding different concentrations of chitosan on the growth performance, body composition and non-specific immune function of juvenile yellow catfish (Pelteobagrus fulvidraco). Four kinds of experimental diets were respectively prepared by adding 0 (control group), 5, 10 and 15 g/kg of chitosan to the basal feed and fed to juvenile yellow catfish for 8 weeks. Results show that the body weight gain rate, specific growth rate, survival rate, body protein content, serum superoxide dismutase activity, catalase activity, glutathione peroxidise activity, lysozyme activity and disease resista...
Source: International Journal of Biological Macromolecules - July 15, 2022 Category: Biochemistry Authors: Shengfu Li Chengxuan Li Shengjun Wu Source Type: research