A novel portable label-free electrochemical immunosensor for ultrasensitive detection of Aeromonas salmonicida in aquaculture seawater
Anal Bioanal Chem. 2022 Aug 2. doi: 10.1007/s00216-022-04219-9. Online ahead of print.ABSTRACTInfectious diseases caused by Aeromonas salmonicida (A. salmonicida) have a huge impact and produce significant losses in aquaculture and fish farming. Fish pathogen early detection is a critical step for the rapid identification and prevention of these problems. This work presents a novel portable label-free ultrasensitive electrochemical immunosensor for A. salmonicida detection in seawater. It consists of a fluidic integrated electrochemical-cell-chip (ECC) with independent chambers enclosing three electrochemical cells (ECs). ...
Source: Analytical and Bioanalytical Chemistry - August 1, 2022 Category: Chemistry Authors: Najib Ben Messaoud Mar ília Barreiros Dos Santos Ana Vieira Alejandro Garrido-Maestu Bego ña Espiña Raquel B Queir ós Source Type: research

A novel portable label-free electrochemical immunosensor for ultrasensitive detection of Aeromonas salmonicida in aquaculture seawater
Anal Bioanal Chem. 2022 Aug 2. doi: 10.1007/s00216-022-04219-9. Online ahead of print.ABSTRACTInfectious diseases caused by Aeromonas salmonicida (A. salmonicida) have a huge impact and produce significant losses in aquaculture and fish farming. Fish pathogen early detection is a critical step for the rapid identification and prevention of these problems. This work presents a novel portable label-free ultrasensitive electrochemical immunosensor for A. salmonicida detection in seawater. It consists of a fluidic integrated electrochemical-cell-chip (ECC) with independent chambers enclosing three electrochemical cells (ECs). ...
Source: Analytical and Bioanalytical Chemistry - August 1, 2022 Category: Chemistry Authors: Najib Ben Messaoud Mar ília Barreiros Dos Santos Ana Vieira Alejandro Garrido-Maestu Bego ña Espiña Raquel B Queir ós Source Type: research

A novel portable label-free electrochemical immunosensor for ultrasensitive detection of Aeromonas salmonicida in aquaculture seawater
Anal Bioanal Chem. 2022 Aug 2. doi: 10.1007/s00216-022-04219-9. Online ahead of print.ABSTRACTInfectious diseases caused by Aeromonas salmonicida (A. salmonicida) have a huge impact and produce significant losses in aquaculture and fish farming. Fish pathogen early detection is a critical step for the rapid identification and prevention of these problems. This work presents a novel portable label-free ultrasensitive electrochemical immunosensor for A. salmonicida detection in seawater. It consists of a fluidic integrated electrochemical-cell-chip (ECC) with independent chambers enclosing three electrochemical cells (ECs). ...
Source: Analytical and Bioanalytical Chemistry - August 1, 2022 Category: Chemistry Authors: Najib Ben Messaoud Mar ília Barreiros Dos Santos Ana Vieira Alejandro Garrido-Maestu Bego ña Espiña Raquel B Queir ós Source Type: research

The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilic Aeromonas salmonida SRW-OG1
In this study, by constructing the mutants of tatA, tatB, and tatC, we investigated the mechanisms underlying temperature-dependent virulence regulation in mesophilic A. salmonida SRW-OG1. According to our results, tatA, tatB, and tatC mutants presented a distinct reduction in adhesion, hemolysis, biofilm formation and motility. Compared to wild-type strain, inhibition of the expression of tatA, tatB, and tatC resulted in a decrease in biofilm formation by about 23.66%, 19.63% and 40.13%, and a decrease in adhesion ability by approximately 77.69%, 80.41% and 62.14% compared with that of the wild-type strain. Furthermore, t...
Source: Frontiers in cellular and infection microbiology - August 1, 2022 Category: Microbiology Source Type: research

Nile tilapia TLR3 recruits MyD88 and TRIF as adaptors and is involved in the NF- κB pathway in the immune response
Int J Biol Macromol. 2022 Jul 28:S0141-8130(22)01639-7. doi: 10.1016/j.ijbiomac.2022.07.201. Online ahead of print.ABSTRACTTLR3 plays a crucial role in innate immunity. In the present study, OnTLR3 was identified in the Nile tilapia Oreochromis niloticus, with a conserved LRR domain and a C-terminal TIR domain. OnTLR3 was broadly expressed in all tissues tested, with the highest expression levels in the blood and the lowest in the kidney. TLR3 mRNA could be detected from pharyngula (2.5 dpf) to late larva (8.5 dpf) during embryonic and larval development. Moreover, the expression level of OnTLR3 was clearly altered in all ...
Source: International Journal of Biological Macromolecules - July 31, 2022 Category: Biochemistry Authors: Fengying Gao Jicai Pang Maixin Lu Zhigang Liu Miao Wang Xiaoli Ke Mengmeng Yi Jianmeng Cao Source Type: research

Metagenomic insights to understand the role of polluted river Yamuna in shaping the gut microbial communities of two invasive fish species
Arch Microbiol. 2022 Jul 20;204(8):509. doi: 10.1007/s00203-022-03127-x.ABSTRACTThe gastrointestinal microbial community plays a crucial role in host health, immunity, protection, development and provides nutrients to the host. The rising human-induced pollution and heavy metal contamination in all aquatic systems globally has led us to explore the gut microbial diversity of two exotic invasive fish Cyprinus carpio (Linnaeus, 1858) and Oreochromis niloticus (Linnaeus,1857) from river Yamuna, India. These fishes are aquatic bioindicators with high demographic resilience. Exploring these associations would pave the way for a...
Source: Archives of Microbiology - July 21, 2022 Category: Microbiology Authors: Meghali Bharti Shekhar Nagar Himani Khurana Ram Krishan Negi Source Type: research

High-fat diet blunts T-cell responsiveness in Nile tilapia
In this study, using Nile tilapia (Oreochromis niloticus) as a model, we investigated the effects of HFD on the primordial T-cell response in fish. Tilapia fed with an HFD for 8 weeks showed impaired lymphocyte homeostasis in the spleen, as indicated by the decreased number of both T and B lymphocytes and increased transcription of proinflammatory cytokines interferon-γ and interleukin-6. Moreover, lymphocytes isolated from HFD-fed fish or cultured in lipid-supplemented medium exhibited diminished T-cell activation in response to CD3ε monoclonal antibody stimulation. Moreover, HFD-fed tilapia infected by Aeromonas hydrop...
Source: Developmental and Comparative Immunology - July 21, 2022 Category: Allergy & Immunology Authors: Kunming Li Jiahua Zhu Kang Li Wei Liang Jiansong Zhang Qian Zhang Xinying Jiao Xiaodan Wang Xiumei Wei Jialong Yang Source Type: research

Metagenomic insights to understand the role of polluted river Yamuna in shaping the gut microbial communities of two invasive fish species
Arch Microbiol. 2022 Jul 20;204(8):509. doi: 10.1007/s00203-022-03127-x.ABSTRACTThe gastrointestinal microbial community plays a crucial role in host health, immunity, protection, development and provides nutrients to the host. The rising human-induced pollution and heavy metal contamination in all aquatic systems globally has led us to explore the gut microbial diversity of two exotic invasive fish Cyprinus carpio (Linnaeus, 1858) and Oreochromis niloticus (Linnaeus,1857) from river Yamuna, India. These fishes are aquatic bioindicators with high demographic resilience. Exploring these associations would pave the way for a...
Source: Archives of Microbiology - July 21, 2022 Category: Microbiology Authors: Meghali Bharti Shekhar Nagar Himani Khurana Ram Krishan Negi Source Type: research

High-fat diet blunts T-cell responsiveness in Nile tilapia
In this study, using Nile tilapia (Oreochromis niloticus) as a model, we investigated the effects of HFD on the primordial T-cell response in fish. Tilapia fed with an HFD for 8 weeks showed impaired lymphocyte homeostasis in the spleen, as indicated by the decreased number of both T and B lymphocytes and increased transcription of proinflammatory cytokines interferon-γ and interleukin-6. Moreover, lymphocytes isolated from HFD-fed fish or cultured in lipid-supplemented medium exhibited diminished T-cell activation in response to CD3ε monoclonal antibody stimulation. Moreover, HFD-fed tilapia infected by Aeromonas hydrop...
Source: Developmental and Comparative Immunology - July 21, 2022 Category: Allergy & Immunology Authors: Kunming Li Jiahua Zhu Kang Li Wei Liang Jiansong Zhang Qian Zhang Xinying Jiao Xiaodan Wang Xiumei Wei Jialong Yang Source Type: research

High-fat diet blunts T-cell responsiveness in Nile tilapia
In this study, using Nile tilapia (Oreochromis niloticus) as a model, we investigated the effects of HFD on the primordial T-cell response in fish. Tilapia fed with an HFD for 8 weeks showed impaired lymphocyte homeostasis in the spleen, as indicated by the decreased number of both T and B lymphocytes and increased transcription of proinflammatory cytokines interferon-γ and interleukin-6. Moreover, lymphocytes isolated from HFD-fed fish or cultured in lipid-supplemented medium exhibited diminished T-cell activation in response to CD3ε monoclonal antibody stimulation. Moreover, HFD-fed tilapia infected by Aeromonas hydrop...
Source: Developmental and Comparative Immunology - July 21, 2022 Category: Allergy & Immunology Authors: Kunming Li Jiahua Zhu Kang Li Wei Liang Jiansong Zhang Qian Zhang Xinying Jiao Xiaodan Wang Xiumei Wei Jialong Yang Source Type: research

Influence of polyamine production and proteolytic activities of co-cultivated bacteria on histamine production by Morganiella morganii
In this study, the histamine forming potential of M. morganii (BSS142) is evaluated when it is co-incubated with proteolytic and polyamine forming bacteria. This experiment was designed to examine whether biotic factors such as proteolysis and the presence of other amines influenced histamine forming ability of BSS142. The study showed that the proteolytic activity of Aeromonas hydrophila as well as Pseudomonas aeruginosa greatly enhanced the histamine forming ability of M. morganii. Psychrobacter sangunis, a non proteolytic polyamine producer, negatively influenced histamine production by M. morganii.PMID:35858814 | DOI:1...
Source: Journal of General and Applied Microbiology - July 20, 2022 Category: Microbiology Authors: Suma Devivilla Manjusha Lekshmi Fathima Salam Sanath Kumar H Rajendran Kooloth Valappil Sibnarayan Dam Roy Binaya Bhusan Nayak Source Type: research