Characterizing a subtropical hypereutrophic lake: From physicochemical variables to shotgun metagenomic data
In this study, shotgun metagenomic sequencing was used to examine the taxonomic and functional structure of microbial communities in Lake Cajititlán during the rainy season. Several water quality features and their interactions with microbial communities were also assessed to identify the major factors affecting the water quality and biota, specifically fish species. According to current water quality regulations, most of the physicochemical variables analyzed (dissolved oxygen, pH, Secchi disk, NH4+, NO3−, blue-green algae, total phosphorus, and chlorophyll-a) were outside of the permissible limits. Planktothrix agardh...
Source: Frontiers in Microbiology - December 2, 2022 Category: Microbiology Source Type: research

GSE211697 Effect of conditioned media from Aeromonas caviae on the transcriptomic changes of the porcine isolates of Pasteurella multocida
Contributors : Teerasak E-kobon ; Nonzee Hanchanachai ; Pramote ChumnanpuenSeries Type : Expression profiling by high throughput sequencingOrganism : Pasteurella multocidaTo investigate the interaction between two porcine isolates of P. multocida (PM2 for type D and PM7 for type A) with Aeromonas caviae by co-culturing P. multocida in the conditioned media prepared from A. caviae (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - December 1, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Pasteurella multocida Source Type: research

The early function of cortisol in liver during Aeromonas hydrophila infection: Dynamics of the transcriptome and accessible chromatin landscapes
In this study, liver transcriptome (RNA-seq) and chromatin accessibility (ATAC-seq) analyses were employed to investigate the early functional role of cortisol in Aeromonas hydrophila-stimulated responses. Our experiments confirmed that A. hydrophila infection can initially significantly increase serum cortisol levels at 1 h after infection. At this time point, the increased serum cortisol levels can significantly regulate A. hydrophila-regulated genes by affecting both transcriptome and chromatin accessibility. Cross-analysis of RNA-seq and ATAC-seq revealed that a certain gene group (92 target_DEGs) was regulated at an ...
Source: Frontiers in Immunology - December 1, 2022 Category: Allergy & Immunology Source Type: research

Characterization of virulence and antimicrobial resistance genes of Aeromonas media strain SD/21 –15 from marine sediments in comparison with other Aeromonas spp.
In this study, we used whole genome sequencing to profile all antimicrobial resistance (AMR) and virulence genes found in A. media strain SD/21–15 isolated from marine sediments in Denmark. To gain a better understanding of virulence and AMR genes found in several A. media strains, we included 24 whole genomes retrieved from the public databanks whose isolates originate from different host species and environmental samples from Asia, Europe, and North America. We also compared the virulence genes of strain SD/21–15 with A. hydrophila, A. veronii, and A. salmonicida reference strains. We detected Msh pili, tap IV pili, ...
Source: Frontiers in Microbiology - December 1, 2022 Category: Microbiology Source Type: research

Aeromonas species isolated from aquatic organisms, insects, chicken, and humans in India show similar antimicrobial resistance profiles
In this study, we used whole genome sequencing (WGS) to identify and compare the antimicrobial resistance (AMR) genes, integrons, transposases and plasmids found in Aeromonas hydrophila, Aeromonas caviae and Aeromonas veronii isolated from Indian major carp (Catla catla), Indian carp (Labeo rohita), catfish (Clarias batrachus) and Nile tilapia (Oreochromis niloticus) sampled in India. To gain a wider comparison, we included 11 whole genome sequences of Aeromonas spp. from different host species in India deposited in the National Center for Biotechnology Information (NCBI). Our findings show that all 15 Aeromonas sequences ...
Source: Frontiers in Microbiology - December 1, 2022 Category: Microbiology Source Type: research

A systematic review and meta-analysis on antimicrobial resistance in marine bivalves
Bivalves are filter-feeding animals able to accumulate contaminants and microorganisms, either of marine or terrestrial origin. The aim of this study was to describe the prevalence of antimicrobial resistance (AMR) in bacterial isolates from bivalves using a systematic review of the literature. Comprehensive searches of MEDLINE, EMBASE, and Web of Science were carried out, based upon a registered protocol (PROSPERO), and following the preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) guidelines. The methodological quality of the included studies was assessed using a modified Hoy checklist. Meta-an...
Source: Frontiers in Microbiology - December 1, 2022 Category: Microbiology Source Type: research

Removal of bacterial pathogens and antibiotic resistance bacteria by anaerobic sludge digestion with thermal hydrolysis pre-treatment and alkaline stabilization post-treatment
Chemosphere. 2022 Nov 24:137383. doi: 10.1016/j.chemosphere.2022.137383. Online ahead of print.ABSTRACTPrimary sludge (PS) is associated with public health and environmental risks, so regulations focus on reducing the pathogenic and heavy metal contents of the treated material (biosolids), intended for soil amendments and land reclamation. The regulations set limits for Escherichia coli (or fecal coliforms), Salmonella spp., helminth eggs and enterovirus. However, the potential risk due to antibiotic resistant bacteria (ARB) and other human potential pathogenic bacteria (HPB) are not considered. In this work, three sludge ...
Source: Chemosphere - November 27, 2022 Category: Chemistry Authors: Yovany Cuetero-Mart ínez Aar ón Flores-Ramírez Daniel De Los Cobos-Vasconcelos Jos é Félix Aguirre-Garrido Yolanda L ópez-Vidal Adalberto Noyola Source Type: research

Removal of bacterial pathogens and antibiotic resistance bacteria by anaerobic sludge digestion with thermal hydrolysis pre-treatment and alkaline stabilization post-treatment
Chemosphere. 2022 Nov 24:137383. doi: 10.1016/j.chemosphere.2022.137383. Online ahead of print.ABSTRACTPrimary sludge (PS) is associated with public health and environmental risks, so regulations focus on reducing the pathogenic and heavy metal contents of the treated material (biosolids), intended for soil amendments and land reclamation. The regulations set limits for Escherichia coli (or fecal coliforms), Salmonella spp., helminth eggs and enterovirus. However, the potential risk due to antibiotic resistant bacteria (ARB) and other human potential pathogenic bacteria (HPB) are not considered. In this work, three sludge ...
Source: Chemosphere - November 27, 2022 Category: Chemistry Authors: Yovany Cuetero-Mart ínez Aar ón Flores-Ramírez Daniel De Los Cobos-Vasconcelos Jos é Félix Aguirre-Garrido Yolanda L ópez-Vidal Adalberto Noyola Source Type: research

Removal of bacterial pathogens and antibiotic resistance bacteria by anaerobic sludge digestion with thermal hydrolysis pre-treatment and alkaline stabilization post-treatment
Chemosphere. 2022 Nov 24:137383. doi: 10.1016/j.chemosphere.2022.137383. Online ahead of print.ABSTRACTPrimary sludge (PS) is associated with public health and environmental risks, so regulations focus on reducing the pathogenic and heavy metal contents of the treated material (biosolids), intended for soil amendments and land reclamation. The regulations set limits for Escherichia coli (or fecal coliforms), Salmonella spp., helminth eggs and enterovirus. However, the potential risk due to antibiotic resistant bacteria (ARB) and other human potential pathogenic bacteria (HPB) are not considered. In this work, three sludge ...
Source: Chemosphere - November 27, 2022 Category: Chemistry Authors: Yovany Cuetero-Mart ínez Aar ón Flores-Ramírez Daniel De Los Cobos-Vasconcelos Jos é Félix Aguirre-Garrido Yolanda L ópez-Vidal Adalberto Noyola Source Type: research

Removal of bacterial pathogens and antibiotic resistance bacteria by anaerobic sludge digestion with thermal hydrolysis pre-treatment and alkaline stabilization post-treatment
Chemosphere. 2022 Nov 24:137383. doi: 10.1016/j.chemosphere.2022.137383. Online ahead of print.ABSTRACTPrimary sludge (PS) is associated with public health and environmental risks, so regulations focus on reducing the pathogenic and heavy metal contents of the treated material (biosolids), intended for soil amendments and land reclamation. The regulations set limits for Escherichia coli (or fecal coliforms), Salmonella spp., helminth eggs and enterovirus. However, the potential risk due to antibiotic resistant bacteria (ARB) and other human potential pathogenic bacteria (HPB) are not considered. In this work, three sludge ...
Source: Chemosphere - November 27, 2022 Category: Chemistry Authors: Yovany Cuetero-Mart ínez Aar ón Flores-Ramírez Daniel De Los Cobos-Vasconcelos Jos é Félix Aguirre-Garrido Yolanda L ópez-Vidal Adalberto Noyola Source Type: research

Variations in antibiotic resistance genes and microbial community in sludges passing through biological nutrient removal and anaerobic digestion processes in municipal wastewater treatment plants
Chemosphere. 2022 Nov 22:137362. doi: 10.1016/j.chemosphere.2022.137362. Online ahead of print.ABSTRACTAntimicrobial resistance (AMR) represents a relentless, silent pandemic. Contributing to this are wastewater treatment plants (WWTPs), a potential source of antibiotic resistance genes' (ARGs) transmission to the environment, threatening public health. The presence of ARGs in pathogenic bacteria and their release into the environment by WWTPs threatens the public health. The current study investigated changes in ARGs' abundance in biological nutrient removal (BNR) processes and anaerobic digestion (AD) reactors of two WWT...
Source: Chemosphere - November 25, 2022 Category: Chemistry Authors: Shahbaz Raza Kyeong Hwan Kang Juhee Shin Seung Gu Shin Jihyun Chun Hyun Uk Cho Jingyeong Shin Young Mo Kim Source Type: research

Variations in antibiotic resistance genes and microbial community in sludges passing through biological nutrient removal and anaerobic digestion processes in municipal wastewater treatment plants
Chemosphere. 2022 Nov 22:137362. doi: 10.1016/j.chemosphere.2022.137362. Online ahead of print.ABSTRACTAntimicrobial resistance (AMR) represents a relentless, silent pandemic. Contributing to this are wastewater treatment plants (WWTPs), a potential source of antibiotic resistance genes' (ARGs) transmission to the environment, threatening public health. The presence of ARGs in pathogenic bacteria and their release into the environment by WWTPs threatens the public health. The current study investigated changes in ARGs' abundance in biological nutrient removal (BNR) processes and anaerobic digestion (AD) reactors of two WWT...
Source: Chemosphere - November 25, 2022 Category: Chemistry Authors: Shahbaz Raza Kyeong Hwan Kang Juhee Shin Seung Gu Shin Jihyun Chun Hyun Uk Cho Jingyeong Shin Young Mo Kim Source Type: research

Immunoprotective role of aloin and disease resistance in Labeo rohita, infected with bacterial fish pathogen, Aeromonas hydrophila
Environ Sci Pollut Res Int. 2022 Nov 24. doi: 10.1007/s11356-022-24253-5. Online ahead of print.ABSTRACTThe effect of aloin on mucosal immune response and disease resistance was elucidated in Labeo rohita infected with the bacterial fish pathogen, Aeromonas hydrophila. Fishes were divided into four groups: (i) control, (ii) vehicle control, (iii) bacteria infected and (iv) bacteria infected and aloin treated. Fish were intraperitoneally injected with A. hydrophila suspension at the dose of 2 × 106 CFU/fish at 0 day (d). Following bacterial injection at 0 d, fish were treated with aloin at a dose of 1 mg/kg body weight int...
Source: Environmental Science and Pollution Research International - November 25, 2022 Category: Environmental Health Authors: Ayan Srivastava Usha Kumari Swati Mittal Ajay Kumar Mittal Source Type: research

Variations in antibiotic resistance genes and microbial community in sludges passing through biological nutrient removal and anaerobic digestion processes in municipal wastewater treatment plants
Chemosphere. 2022 Nov 22:137362. doi: 10.1016/j.chemosphere.2022.137362. Online ahead of print.ABSTRACTAntimicrobial resistance (AMR) represents a relentless, silent pandemic. Contributing to this are wastewater treatment plants (WWTPs), a potential source of antibiotic resistance genes' (ARGs) transmission to the environment, threatening public health. The presence of ARGs in pathogenic bacteria and their release into the environment by WWTPs threatens the public health. The current study investigated changes in ARGs' abundance in biological nutrient removal (BNR) processes and anaerobic digestion (AD) reactors of two WWT...
Source: Chemosphere - November 25, 2022 Category: Chemistry Authors: Shahbaz Raza Kyeong Hwan Kang Juhee Shin Seung Gu Shin Jihyun Chun Hyun Uk Cho Jingyeong Shin Young Mo Kim Source Type: research

Variations in antibiotic resistance genes and microbial community in sludges passing through biological nutrient removal and anaerobic digestion processes in municipal wastewater treatment plants
Chemosphere. 2022 Nov 22:137362. doi: 10.1016/j.chemosphere.2022.137362. Online ahead of print.ABSTRACTAntimicrobial resistance (AMR) represents a relentless, silent pandemic. Contributing to this are wastewater treatment plants (WWTPs), a potential source of antibiotic resistance genes' (ARGs) transmission to the environment, threatening public health. The presence of ARGs in pathogenic bacteria and their release into the environment by WWTPs threatens the public health. The current study investigated changes in ARGs' abundance in biological nutrient removal (BNR) processes and anaerobic digestion (AD) reactors of two WWT...
Source: Chemosphere - November 25, 2022 Category: Chemistry Authors: Shahbaz Raza Kyeong Hwan Kang Juhee Shin Seung Gu Shin Jihyun Chun Hyun Uk Cho Jingyeong Shin Young Mo Kim Source Type: research