IL-23 Enhances C-Fiber-Mediated and Blue Light-Induced Spontaneous Pain in Female Mice

The incidence of chronic pain is especially high in women, but the underlying mechanisms remain poorly understood. Interleukin-23 (IL-23) is a pro-inflammatory cytokine and contributes to inflammatory diseases (e.g., arthritis and psoriasis) through dendritic/T cell signaling. Here we examined the IL-23 involvement in sexual dimorphism of pain, using an optogenetic approach in transgenic mice expressing channelrhodopsin-2 (ChR2) in TRPV1-positive nociceptive neurons. In situ hybridization revealed that compared to males, females had a significantly larger portion of small-sized (100-200 μm2) Trpv1+ neurons in dorsal root ganglion (DRG). Blue light stimulation of a hindpaw of transgenic mice induced intensity-dependent spontaneous pain. At the highest intensity, females showed more intense spontaneous pain than males. Intraplantar injection of IL-23 (100 ng) induced mechanical allodynia in females only but had no effects on paw edema. Furthermore, intraplantar IL-23 only potentiated blue light-induced pain in females, and intrathecal injection of IL-23 also potentiated low-dose capsaicin (500 ng) induced spontaneous pain in females but not males. IL-23 expresses in DRG macrophages of both sexes. Intrathecal injection of IL-23 induced significantly greater p38 phosphorylation (p-p38), a marker of nociceptor activation, in DRGs of female mice than male mice. In THP-1 human macrophages estrogen and chemotherapy co-application increased IL-23 secretion, and furthermore, estroge...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research

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CONCLUSION: In our sample, percentages of variation in PLR and PD were found to be directly representative of TBI patients’ fentanyl blood concentration. Considering information about blood drug concentration is generally not available at bedside, PLR could be used as a proxy to assess analgesia requirements before a nociceptive procedure in critically ill sedated TBI patients who are vulnerable to stress.
Source: Journal of Neuroscience Nursing - Category: Neuroscience Tags: Articles Source Type: research
People who experience debilitating back pain come to their doctors with one pressing question: Do I need back surgery? Often, they are surprised to hear me, a neurosurgeon, answer, “No.” In many cases, physical therapy, pain management, weight loss management, and physiatry can help people to delay – and in some cases avoid – backRead more …Expertise is the backbone of successful spine treatment originally appeared inKevinMD.com.
Source: Kevin, M.D. - Medical Weblog - Category: General Medicine Authors: Tags: Conditions Neurosurgery Orthopedics Source Type: blogs
DURING the pandemic, the public has become used to hearing about the effects of COVID-19 and how it effects the lungs and the heart. It isn't the only non-cancerous condition that can cause severe damage to these organs. Rheumatoid Arthritis can cause serious lung complications.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
CONCLUSION: Overall, another AML family with CEBPA gene mutation is added to the literature, presenting with yet unreported FAB subtype M5 and absence of CD7 expression in some family members.PMID:35032366 | DOI:10.1002/mgg3.1854
Source: Molecular Medicine - Category: Molecular Biology Authors: Source Type: research
CONCLUSION: With 73.2% recurrence, corticosteroid injections do not appear to be effective in treating dorsal ganglion cysts of the wrist. They lead to an improvement in the functional score of the wrist, but not significantly compared to treatment abstinence.LEVEL OF EVIDENCE: IV.PMID:35031514 | DOI:10.1016/j.otsr.2022.103198
Source: Hand Surgery - Category: Surgery Authors: Source Type: research
CONCLUSIONS: The free antegrade palmaris longus tendocutaneous venous flap enabled the repair of compound tissue defects of the dorsal digit, resulting in acceptable aesthetics, repaired tendon defects, and wound coverage. This surgical method is an option for the reconstruction of small compound defects of digits with extensor defects.TYPE OF STUDY/LEVEL OF EVIDENCE: Therapeutic IV.PMID:35031123 | DOI:10.1016/j.jhsa.2021.11.023
Source: Hand Surgery - Category: Surgery Authors: Source Type: research
Comp Biochem Physiol C Toxicol Pharmacol. 2022 Jan 11:109266. doi: 10.1016/j.cbpc.2022.109266. Online ahead of print.ABSTRACTA large number of people worldwide are affected by chronic metal exposure, which is known to be associated with different type of malignancies. The mechanisms of metal carcinogenicity are complex in nature, and excessive reactive oxygen species (ROS) generation induced by chronic metal exposure, among the other factors, has been proposed as one of the major mechanisms involved in that process. In tumor cells, ROS buildup may lead to cell death through intrinsic and extrinsic signaling pathways. Furth...
Source: Comparative biochemistry and physiology. Toxicology and pharmacology : CBP - Category: Biochemistry Authors: Source Type: research
Pflugers Arch. 2022 Jan 15. doi: 10.1007/s00424-021-02656-6. Online ahead of print.ABSTRACTSensory neurons are responsible for the generation and transmission of nociceptive signals from the periphery to the central nervous system. They encompass a broadly heterogeneous population of highly specialized neurons. The understanding of the molecular choreography of individual subpopulations is essential to understand physiological and pathological pain states. Recently, it became evident that species differences limit transferability of research findings between human and rodents in pain research. Thus, it is necessary to syst...
Source: Pflugers Archiv : European Journal of Physiology - Category: Physiology Authors: Source Type: research
Queen Elizabeth II’s decision to strip Prince Andrew of his military titles and patronages was a brutal and humiliating exercise in damage-limitation, royal experts said. #elizabethii
Source: Reuters: Health - Category: Consumer Health News Source Type: news
ConclusionsChildhood cancer survivors have more missed opportunities for concomitant HPV vaccination than a population sample. As flu shots should be administered annually, providers have a regular opportunity to recommend and deliver the HPV vaccine to survivors.
Source: Cancer Medicine - Category: Cancer & Oncology Authors: Tags: RESEARCH ARTICLE Source Type: research
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