GSE152422 Visualizing programmed lineage trajectories at cancer initiation

Contributors : Joshua D Ginzel ; Chaitanya R Acharya ; Veronica Lubkov ; Hidetoshi Mori ; Peter G Boone ; Lauren K Rochelle ; Wendy Roberts ; Zachary C Hartman ; Erika Crosby ; Jeffrey I Everitt ; Lawrence S Barak ; Marc G Caron ; Jane Q Chen ; Neil E Hubbard ; Robert D Cardiff ; Alexander D Borowsky ; H K Lyerly ; Joshua C SnyderSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe sequential accumulation of survival advantage traits – the cancer evolution hypothesis – implies that early detection and treatment of precancers can prevent cancer mortality. In practice, detection and treatment of breast precancer ductal  carcinoma in situ (DCIS) has had minimal impact on reducing breast cancer mortality. The evolution of preca ncer is not inevitable, suggesting that inciting neoplastic events lead to different trajectories of progression. Here we visualize distinct tumor driver gene programs of lineage specification occurring at cancer initiation. Crainbow fluorescent barcoding of wild-type HER2, d16-HER2, and p95-HER2 en ables whole-gland lineage tracing and single-cell reconstruction of the origin of HER2+ breast cancer. Molecular pathology and Crainbow lineage tracing provide genotype:phenotype modules and gene signatures predictive of cancer progression. WT-HER2 neoplasms were rare and proliferative d16-HER2 neop lasms eventually progressed to metastasis, and p95-HER2 induced rapidly invasive and metastatic carcinomas. p95-HER2 ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research