Targeting stanniocalcin ‐1‐expressing tumor cells elicits efficient antitumor effects in a mouse model of human lung cancer
This study elucidated that STC‐1 could be useful as both a molecular target and as a marker for lung cancer. AbstractLung cancer is the most common cause of cancer ‐related death in developed countries; therefore, the generation of effective targeted therapeutic regimens is essential. Recently, gene therapy approaches toward malignant cells have emerged as attractive molecular therapeutics. Previous studies have indicated that stanniocalcin‐1 (STC‐1), a hormone involved in calcium and phosphate homeostasis, positively regulates proliferation, apoptosis resistance, and glucose metabolism in lung cancer cell lines. In this study, we investigated if targeting STC‐1 in tumor cells could be a promising strategy for lung cancer gene therapy. We confirm ed that STC‐1 levels in peripheral blood were higher in lung cancer patients than in healthy donors and that STC‐1 expression was observed in five out of eight lung cancer cell lines. A vector expressing a suicide gene, uracil phosphoribosyltransferase (UPRT), under the control of the STC‐1 pr omoter, was constructed (pPSTC ‐1‐UPRT) and transfected into three STC‐1‐positive cell lines, PC‐9, A549, and H1299. When stably transfected, we observed significant cell growth inhibition using 5‐fluorouracil (5‐FU) treatment. Furthermore, growth of the STC‐1‐negative lung cancer cell line, LK‐2 was significantly arrested when combined with STC‐1‐positive cells transfected with pPSTC ‐1‐UPRT.We belie...
Source: Cancer Medicine - Category: Cancer & Oncology Authors: Kotaro Abe,
Masahiko Kanehira,
Shinya Ohkouchi,
Sakiko Kumata,
Yamato Suzuki,
Hisashi Oishi,
Masafumi Noda,
Akira Sakurada,
Eisaku Miyauchi,
Tohru Fujiwara,
Hideo Harigae,
Yoshinori Okada Tags: ORIGINAL RESEARCH Source Type: research
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