The Effect of miR-361-3p Targeting TRAF6 on Apoptosis of Multiple Myeloma Cells.

The Effect of miR-361-3p Targeting TRAF6 on Apoptosis of Multiple Myeloma Cells. J Microbiol Biotechnol. 2020 Dec 14;: Authors: Fan Z, Wu Z, Yang B Abstract microRNA-361-3p (miR-361-3p) is involved in carcinogenesis of oral cancer and pancreatic catheter adenocarcinoma, and has anti-carcinogenic effects on non-small cell lung cancer (NSCLC), however, its effect on multiple myeloma (MM) is less reported. The current study found that up-regulating the expression of miR-361-3p inhibited MM cell viability and promoted MM apoptosis. Expressions of tumour necrosis factor receptor-associated factor 6 (TRAF6) and miR-361-3p of MM cells were measured. In addition, the viability, colony formation rate, apoptosis of MM cells were detected, and expressions of apoptosis-related genes Bcl-2, Bax, Cleaved caspase-3 (C caspase-3) were measured. Furthermore, the binding site between miR-361-3p and TRAF6 was predicted by TargetScan. The results showed that miR-361-3p was low expressed in plasma of MM patients and cell lines, while its overexpression inhibited viability and colony formation of MM cells and increased the cell apoptosis. Furthermore, TRAF6, which was predicted to be a target gene of miR-361-3p, was high-expressed in plasma of patients and cell lines with MM. Rescue experiments demonstrated that effect of TRAF6 on MM cells was opposite to that of miR-361-3p. Up-regulation of miR-361-3p induced apoptosis and inhibited cell proliferation of...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research