Upregulation of Bromodomain PHD ‑finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival.

Upregulation of Bromodomain PHD‑finger transcription factor in ovarian cancer and its critical role for cancer cell proliferation and survival. Biochem Cell Biol. 2020 Sep 27;: Authors: Miao J, Zhang M, Huang X, Xu L, Tang R, Wang H, Han S Abstract Bromodomain PHD finger transcription factor (BPTF) is a core subunit of nucleosome-remodeling factor (NURF) complex, which plays an important role in the development of several cancers. However, it is unknown whether BPTF regulates ovarian cancer (OC) progression. Relative expression of BPTF in cell lines and tissues of OC were measured by Western blot and immunohistochemistry respectively. Clinical significance of BPTF in OC was analyzed by Chisquare test. BPTF knockdown effects on the proliferation, migration, invasion and apoptosis of OC cells were examined. Mechanism studies revealed that these effects were achieved through simultaneous modulation of multiple signaling pathways. We found that BPTF was highly expressed in OC cell lines and tissues compared with normal human ovarian epithelial cell and non-cancerous tissues (p<0.05). These results are also supported by the public RNA-seq data. BPTF overexpression was correlated with poor ovarian cancer patient survival (p<0.05). Vitro experiments revealed that the down-regulation of BPTF inhibited OC cell proliferation, colony formation, migration, invasion and induced its apoptosis. BPTF knockdown also regulated the epithelial-m...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research