An obesogenic maternal environment impairs mouse growth patterns, satellite cell activation and markers of post-natal myogenesis.

In this study, we investigated the effect of an obesogenic maternal environment on growth and post-natal myogenesis in the offspring. Male C57BL/6J mice born to chow or high-fat fed mothers were allocated to four different groups at the end of weaning. For the following ten weeks, half of the pups were maintained on the same diet as their mother and half of the pups were switched to the other diet (chow or high-fat). At 12 weeks of age, muscle injury was induced using an intramuscular injection of barium chloride. Seven days later, mice were humanely killed and muscle tissue was harvested. A high-fat maternal diet impaired offspring growth patterns and downregulated satellite cell activation and markers of post-natal myogenesis seven days after injury without altering the number of newly synthetized fibres over the whole seven-day period. Importantly, a healthy post-natal diet could not reverse any of these effects. In addition, we demonstrated that post-natal myogenesis was associated with a diet-independent upregulation of three miRNAs, mmu-miR-31-5p, mmu-miR-136-5p and mmu-miR-296-5p. Further in vitro analysis confirmed the role of these miRNAs in myocyte proliferation. Our findings are the first to demonstrate that maternal over-nutrition impairs markers of post-natal myogenesis in the offspring, and are particularly relevant to today's society where the incidence of overweight/obesity in women of child-bearing age is increasing. PMID: 32954829 [PubMed - as suppli...
Source: Am J Physiol Endocri... - Category: Endocrinology Authors: Tags: Am J Physiol Endocrinol Metab Source Type: research