Zeb2 regulates the balance between retinal interneurons and muller glia by inhibition of BMP-Smad signaling.

Zeb2 regulates the balance between retinal interneurons and muller glia by inhibition of BMP-Smad signaling. Dev Biol. 2020 Sep 17;: Authors: Menuchin-Lasowski Y, Dagan B, Conidi A, Cohen-Gulkar M, David A, Ehrlich M, Giladi PO, Clark BS, Blackshaw S, Shapira K, Huylebroeck D, Henis Y, Ashery-Padan R Abstract The interplay between signaling molecules and transcription factors during retinal development is key to controlling the correct number of retinal cell types. Zeb2 (Sip1) is a zinc-finger multidomain transcription factor that plays multiple roles in central and peripheral nervous system development. Haploinsufficiency of ZEB2 causes Mowat-Wilson syndrome, a congenital disease characterized by intellectual disability, epilepsy and Hirschsprung disease. In the developing retina, Zeb2 is required for generation of horizontal cells and the correct number of interneurons; however, its potential function in controlling gliogenic versus neurogenic decisions remains unresolved. Here we present cellular and molecular evidence of the inhibition of Muller glia cell fate by Zeb2 in late stages of retinogenesis. Unbiased transcriptomic profiling of control and Zeb2-deficient early-postnatal retina revealed that Zeb2 functions in inhibiting Id1/2/4 and Hes1 gene expression. These neural progenitor factors normally inhibit neural differentiation and promote Muller glia cell fate. Chromatin immunoprecipitation (ChIP) supported direct regulation...
Source: Developmental Biology - Category: Biology Authors: Tags: Dev Biol Source Type: research