IJERPH, Vol. 17, Pages 5551: Method for Malaria Diagnosis Based on Extractions of Samples Using Non-Invasive Techniques: An Opportunity for the Nursing Clinical Practice
IJERPH, Vol. 17, Pages 5551: Method for Malaria Diagnosis Based on Extractions of Samples Using Non-Invasive Techniques: An Opportunity for the Nursing Clinical Practice
International Journal of Environmental Research and Public Health doi: 10.3390/ijerph17155551
Authors:
Adela Gómez-Luque
Juan Carlos Parejo
Maria Zoraida Clavijo-Chamorro
Fidel López-Espuela
Faustin Munyaruguru
Silvia Belinchón Lorenzo
Isabel Monroy
Luis Carlos Gómez-Nieto
Malaria has been for millennia one of the best known and most destructive diseases affecting humans. Its high impact has aroused great interest for the development of new effective and reliable diagnostic techniques. Recently it has been recently published that hairs from mammal hosts are able to capture, hold and finally remove foreign DNA sequences of Leishmania parasites. The aim of this study was to check if Plasmodium falciparum (P. falciparum) DNA remains stable in blood samples deposited in Whatman paper after suffering different transport and storage conditions, and to compare the sensitivity of these results with those offered by thick a smear and Rapid Diagnostic Test, and besides to examine whether P. falciparum DNA would be detected and quantified by Real time quantitative PCR (qPCR) from hairs of people with different types of malaria. P. falciparum Histidine Repeat Protein II (pHRP-II) antigen detection and P. falciparum DNA were detected in 18 of 19 dry blood samples adhered to Whatman paper (94.74%)...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Adela G ómez-Luque Juan Carlos Parejo Maria Zoraida Clavijo-Chamorro Fidel L ópez-Espuela Faustin Munyaruguru Silvia Belinch ón Lorenzo Isabel Monroy Luis Carlos G ómez-Nieto Tags: Article Source Type: research