DAP1 negatively regulates autophagy induced by cardamonin in SKOV3 cells.

This study aimed to investigate whether it is relevant for DAP1 in proliferation inhibition and autophagy induced by cardamonin in tumor cells. Using online bioinformatics tools, we found that DAP1 expression is closely related to the survival of patients with ovarian cancer. Our study showed that autophagy induced by cardamonin was associated with mTOR inhibition, and DAP1 was involved in this process. Silence of DAP1 decreased cell proliferation but enhanced antiproliferative effect of cardamonin in SKOV3 cells. The level of autophagy was elevated by DAP1 silencing in SKOV3 cells. Notably, cardamonin showed higher autophagy flux in DAP1 siRNA group. Taken together, our results implied that DAP1 negatively regulates autophagy induced by cardamonin, and it may be a potential target for ovarian cancer therapy. This article is protected by copyright. All rights reserved.
PMID: 32706448 [PubMed - as supplied by publisher]

Source: Cell Biology International - July 23, 2020 Category: Cytology Authors: Nie X, Chen H, Niu P, Zhu Y, Zhou J, Jiang L, Li D, Lin M, Chen Z, Shi D Tags: Cell Biol Int Source Type: research